Hibiscus Sabdariffa Extract Research Articles

Staining Property of Alcoholic and Aqueous Hibiscus sabdariffa Extract in Demonstration of Selected Bacteria in Tissue Sections of Wistar Rats

Introduction: Dye is used for the artificial colouration of a substance to facilitate its examination by the use of the coloured organic molecule, a process called staining. Over the past many years, it has been observed that synthetic dyes have many disadvantages associated with them like toxicity and allergenicity. This study aimed to determine the staining effect of Hibiscus sabdariffa extracts of various concentration, various pH and duration in bacteria staining compared with Gram’s staining.
 Methods: Standard Gram stains as control and both Hibiscus sabdariffa extracts (alcohol and boiled water) were used to stain inflammatory appendix tissue and lung tissue inoculated with bacteria using various concentration (5% and 10%), at various duration (30 seconds and 1 hour) and with change of pH, achieved by treating the extracts with ammonium hydroxide and glacial acetic acid. Each was used as a counterstain in bacterial stain (to replace Safranin/Neutral red) Safranin/Neutral red.
 Results: All extracts after treatment were acidic but the change of pH was indirectly proportional to the staining ability of the extracts. The Hibiscus solutions gave the background, a brown colouration. And inflammatory cells were demonstrated better than the bacteria with aqueous Hibiscus solution strongly when compared with the alcoholic Hibiscus solution.
 Conclusion: The results obtained indicate that Hibiscus solution has the potential for use in diagnostic bacteriology in formalin-fixed paraffin-embedded tissue section.

Microbial evaluation and some proposed good manufacturing practices of locally prepared malted corn drink (“asaana”) and Hibiscus sabdarifa calyxes extract (“sobolo”) beverages sold at a university cafeteria in Ghana

In most developing countries street food vending remains a growing industry providing daily foods for most urban dwellers. Sorrel drink (Sobolo) is a drink made from Hibiscus sabdariffa extract, while ‘‘Asaana’’ is a refreshing drink made from maize. These drinks are mostly produced on a fairly small scale and usually for local consumption at home or vended on the street. These beverages are now widely patronized drinks sold on the streets of Ghana. Despite their high patronage, microbial contamination from the preparation to packaging raises concerns. This study evaluated the microbial quality of these locally prepared beverages ‘‘Asaana’’ and “Sobolo” sold at the cafeterias of two different campuses of a university in Ghana. For sobolo, total plate count of range 7.60 × 106–1.58 × 108 CFU/ml was recorded while a range of 0–4.8 × 107 CFU/ml was recorded for E.coli. A similar trend was observed for asana. Fungal counts also ranged 2.29–4.86 log10 CFU/ml and 2.098–4.23 log10 CFU/ml for sobolo and asana respectively. All samples collected exceeded tolerable limits of 106 for microorganisms in ready to eat foods. samples analyzed were acidic in nature, most bacteria cannot thrive under this condition with the exception of aciduric bacteria such as lactobacillus species and temperatures of these samples ranged from 9.5 °C to 14.5 °C which is conducive for the survival and growth of microorganisms hence, resulting in high counts of microorganisms in these beverages.

ANTIMICROBIAL AND ANTIRADICAL PROPERTIES OF HAMMADA SCOPARIA (POMEL) ILJIN

Background: Hammada scoparia (Pomel) Iljin (HS), is commonly used by traditional healers in Morocco against microbial and fungal infections. We studied antimicrobial, antifungal and antiradical effects of organic extracts in vitro in order to confirm traditional utilization after phytochemical screening.
 Materials and methods: Aerial parts of HS have been extracted by hydro-distillation using Clevenger-type apparatus, and the chemical composition was realized by Gas Chromatography coupled with Mass Spectroscopy (GC/MS). The antioxidant activity has been evaluated using DPPH test, while the antimicrobial tests of HS extract were conducted on twenty-eight bacterial strains and antifungal on twelve fungal strains.
 Results: Chemical characterization of HS essential oils (EO) confirmed the presence of carvacrol (82,28%), p-cymene (2,52%), γ-terpinene (2,18%) and Z-caryophyllene (2,04%). Antimicrobial tests of HS extract showed a moderate antibacterial activity without antifungal effect. In addition, HS exhibited a very powerful antiradical activity with IC50 = 1,2 mg/ml compared to that of ascorbic acid (IC50 = 0,5 mg/mL) and butylated hydroxyanisole (0,8 mg/mL).
 Conclusion: To our knowledge, this is the first demonstration that HS directly inhibits the growth of microorganisms in vitro, and further validates its traditional use as an antiseptic by traditional Moroccan healers.

Natural Red Dyes From Hibiscuss sabdariffa L. Calyxes Extract For Gamma-Rays Detector

Detection of absorbed dose in materials were irradiated is one method for monitoring irradiation processes. Dyes that are sensitive to ionizing radiation has the potential to used as radiation detectors. This article will report on the fabrication of gamma-rays detector using natural red dyes obtained from Hibiscus sabdariffa L. (HS) calyxes extract. The HS extract solution was made using a simple extraction method which was carried out by soaking the dried HS calyxes with double distilled water as a solvent for 24 hours. Gammacell irradiator with Cobalt-60 radiation source was used to test the sensitivity of HS extract solution to gamma radiation with a dose of 1-10 kGy. UV-Vis spectrophotometer was used to the characterization of optical properties of HS extract solution. After gamma irradiation, it was observed the color of the HS extract solution faded slightly from dark red to more transparent red color along with increasing doses. The results of UV-Vis characterization showed maximum absorbance of HS extract solution at 523 nm and a decrease in absorbance of 95% after irradiation at a dose of 10 kGy. The HS extract solution is stable in the conditions of storage is not exposed light and at the refrigerator up to 30 days.

Hibiscus sabdariffa Extract Inhibits Adhesion, Biofilm Initiation and Formation in Candida albicans.

Microbial biofilms act as reservoirs for pathogenic sessile microbes which reside inside the three dimensional matrix of the biofilm, and are thus protected against anti-microbial drugs. Most of the anti-microbial drugs fail to completely abolish the biofilm associated infections. In the present study, we provide evidence of Hibiscus sabdariffa (Hs) extract having possible anti-microbial activity, with emphasis on Candida albicans biofilm. The Hs extract was shown to be effective against C. albicans pre-formed biofilm at 3.125mg/ml and was able to inhibit the hyphae initiation and adherence of cells. Furthermore, Hs extract was able to reduce the C. albicans load in C. elegans by effectively killing the Candida cells thereby reducing the viable colony count and effectively increasing the lifespan of worms. The percentage of viable hatched progeny of worms exposed to Hs extract (both at conc. 1.5mg/ml and 6.25mg/ml), was also comparable to that of the control untreated eggs. The Hs extract was also found to be significantly effective against fluconazole resistant C. albicans isolated from patients. Thus, we, for the first time, propose Hs extract as a prospective drug candidate and substitute for eradicating pre-formed biofilm and inhibiting the growth of C. albicans.

The protective effect of Hibiscus sabdariffa calyxes extract against cypermethrin induced oxidative stress in mice

Cypermethrin (Cyp) is a kind of pyrethroids compound that is broadly used against different species of insects and pests. Cyp can also elicit a range of neurotoxic, immunotoxic, genotoxic and reproductive toxic effects on various experimental organisms. The aim of this study was to evaluate the protective effects of Hibiscus sabdariffa against the toxicity damage induced by Cyp exposure. The Hibiscus sabdariffa calyxes extract was given to mice (200–500 mg/kg bw). The mice, which were treated with Cyp and Hibiscus sabdariffa, were divided into six groups of six mice each. Groups I, IV and VI were used as control and groups II CYP control (20 mg/kg body weight)., groups III and V were treated with Hibiscus sabdariffa extract (200 and 500 mg/kg body weight) plus (20 mg/kg body weight) for 21 days Furthermore, HPLC was used to identify the compound fraction. This result showed Cyp -induced biochemical changes in all organs of mice. Cyp caused decreased CAT activity, inhibition of AChE activity and increased the levels of H2O2 and MDA in brain, heart, liver and kidney. Hibiscus sabdariffa exhibited antioxidant effect and significantly attenuated the neurotoxicity of Cyp. Hibiscus sabdariffa exhibits neuroprotective effects and can be an effective and novel alternative approach to reduce the risk caused by pyrethroid compound.

Hibiscus ameliorates salt-induced carotid intima-media thickness in albino rats

Objectives: The carotid intima-media thickness (CIMT) is a known biomarker of clinical and subclinical cardiovascular events and evaluation of therapeutic action. Excessive salt directly causes changes in the common carotid intima and media layers and has been linked to hypertensive disease resulting to changes on vascular structure. Hibiscus sabdariffa is a traditional herbal drink with antihypertensive effects. The anatomical aspects of its effects however, are largely unknown. This study therefore, investigated the effects of hibiscus extract on CIMT in rats fed with a high-salt diet. Methods: Young albino rats (Rattus norvegicus) were divided into three groups: (A) high-salt diet alone, (B) high-salt diet and Hibiscus sabdariffa extract and (C) control group fed a normal diet for 8 weeks. Specimens from carotid arteries of rats were fixated and processed for paraffin embedding. Seven-micrometer thick sections were stained with Hematoxylin and Eosin stain and examined under light microscopy. Morphometric measurements were taken to determine the CIMT. Results: High-salt diet increased CIMT from 497.86 µm to 697.85 µm in 8 weeks. In Hibiscus sabdariffa extract fed rats, the CIMT decreased to 542.85 µm (p<0.05) by week 8. Conclusion: Hibiscus sabdariffa extract ameliorates salt-induced increase in CIMT in rats in a time-dependent manner. This implies that Hibiscus sabdariffa products may have therapeutic value in salt-induced vascular morbidity.

Synthesis of ZnO-gold nanoparticles composite using Hibiscus sabdariffa extract as reductor

One of the synthesis methods currently being carried out is green synthesis. One of the efforts to implement the green method is to use plant extracts that can be renewed. Hibiscus sabdariffa is one type of plant that is able to produce anthocyanin pigments. In this study, the synthesis of ZnO composites with gold nanoparticles will be carried out using Hibiscus sabdariffa flower extract. The composites of gold nanoparticles and ZnO were determined using XRD instrumentation where there were characteristic peaks of ZnO and gold nanoparticles.

The change of tissue of inhibitor metalloproteinase 1 (timp-1) after rosella (hibiscus sabdariffa) extract gel application on artificial crown patients with gingivitis

The change of tissue of inhibitor metalloproteinase 1 (timp-1) after rosella (hibiscus sabdariffa) extract gel application on artificial crown patients with gingivitis

Development of a Mucoadhesive and an in Situ Gelling Formulation Based on κ-Carrageenan for Application on Oral Mucosa and Esophagus Walls. II. Loading of a Bioactive Hydroalcoholic Extract.

The aim of the present work was to load a Hibiscus sabdariffa (HS) hydroalcoholic extract into in situ gelling formulations for the treatment of oral mucositis and esophagitis. Such formulations, selected as the most promising options in a previous work of ours, were composed by κ-carrageenan (κ-CG), a sulfated marine polymer able to gelify in presence of saliva ions, hydroxypropyl cellulose (HPC), used as mucoadhesive agent, and CaCl2, salt able to enhance the interaction κ-CG/saliva ions. HS extract, which is rich in phytochemicals such as polyphenols, polysaccharides and organic acids, was selected due to its antioxidant and anti-inflammatory properties. For HS extraction, three different methodologies (maceration, Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE)) were compared in terms of extraction yield and extract antioxidant activity, revealing that MAE was the best procedure. Rheological and mucoadhesive properties of HS-loaded formulations were investigated. Such formulations were characterized by a low viscosity at 25 °C, guaranteeing an easy administration, a proper in situ gelation behavior and marked elastic and mucoadhesive properties at 37 °C, functional to a protective action towards the damaged mucosa. Finally, the biocompatibility and the proliferative effect of HS-loaded formulations, as well as their antioxidant and anti-inflammatory properties, were proved in vitro on human dermal fibroblasts.

Beneficial Effects of Natural Bioactive Compounds from Hibiscus sabdariffa L. on Obesity.

Obesity is a condition associated with the accumulation of excess fat in the body, energy imbalance, lipogenesis, etc., which increases adipose tissue mass through adipogenesis and poses a health risk. Its prevalence has become an economic burden to the health care system and the world at large. One of the alternatives to tackling obesity involves the use of bioactive compounds. We critically examined the effects of Hibiscus sabdariffa extract (HSE) on various parameters associated with the development of obesity such as; the effect of HSE on body weight, the effect of HSE on lipid accumulation, cholesterol metabolism and plasma parameters, the inhibitory effect of HSE on pancreatic lipase, and the effect of HSE on adipocyte differentiation/adipogenesis. This review has gathered reports on the various anti-obesity effects of H. sabdariffa bioactive compounds in cell and animal models, as well as in humans. Available toxicology information on the consumption of H. sabdariffa revealed that its toxicity is dose-dependent and may cause an adverse effect when administered over a long period of time. Reports have shown that H. sabdariffa derived bioactive compounds are potent in the treatment of obesity with an evident reduction in body weight, inhibition of lipid accumulation and suppression of adipogenesis through the PPARγ pathway and other transcriptional factors.

Polyphenols-enriched Hibiscus sabdariffa extract-loaded nanostructured lipid carriers (NLC): Optimization by multi-response surface methodology

Nanoparticles are commonly used to improve the bioavailability of several bioactive compounds. In this work, nanostructured lipid carriers (NLC) have been optimized by response-surface methodology to protect phenolic compounds from Hibiscus sabdariffa. The influence of lipid and surfactant concentration on the mean particle size, polydispersity index and zeta potential of NLC was analysed. The results showed that the effect of both independent variables was statistically significant on the selected responses. The optimized formulation obtained from the predicted model was composed of 2.21% (w/w) of lipid phase and 1.93% (w/w) of surfactant, with a desirability function of 0.77. The loading of polyphenol-enriched Hibiscus sabdariffa extract - obtained by microwave assisted extraction or by pressurized liquid extraction - within the NLC resulted in particles with a mean size of 470 ± 8 nm (encapsulation efficiency of 52.9 ± 0.9% for quercetin and 60 ± 2% for anthocyanins) and 344 ± 12 nm (encapsulation efficiency of 93 ± 3% for quercetin and 84 ± 4% for anthocyanins), respectively. Transmission electron microscopy images and Fourier transformed infra-red analysis of extracts-loaded NLC confirmed that interaction between lipids and polyphenols improved the physicochemical stability of the particles. Our findings highlight the added value of NLC as a delivery system for phenolic compounds extracted from Hibiscus sabdariffa.

412 Synergism between exogenous lactoferrin and dietary Hibiscus sabdariffa extract in prevention of mastitis in dairy goats during the dry period.

412 Synergism between exogenous lactoferrin and dietary Hibiscus sabdariffa extract in prevention of mastitis in dairy goats during the dry period.

Hydrogen-bonding interactions and compostability of bionanocomposite films prepared from corn starch and nano-fillers with and without added Jamaica flower extract

Bionanocomposite films processed by twin screw extrusion followed by thermo molding were prepared from corn starch (Zea mays) and pH-sensitive nano-clays packaged with Jamaica flower (Hibiscus sabdariffa) extract (JFE). The hydrogen (H)-bonding interactions of the materials obtained were evaluated by ATR/FTIR spectroscopy, and their influence on the physicochemical and surface properties of the materials was analyzed. The degree of biodegradability and compostability of the films was also recorded. This latter was analyzed in terms of the ecotoxicity of the films using the variations in the growth of the primary root of lettuce (Lactuca sativa) seedlings exposed to three concentrations (1, 10 and 100 μg/mL) of the powdered films as a biomarker. The addition of the JFE-containing nano-fillers strengthened the H-bonding interactions with the thermoplastic starch (TPS) matrix, and these interactions were more efficient when there were fewer steric impediments between the JFE and the TPS. Additionally, stronger H-bonding interactions produced more hydrophilic surfaces, with greater surface energy and rougher surface morphology. All the films tested were biodegradable. Our research group had previously encountered high cytotoxicity in one of the evaluated nano-clay systems, and in this study, we confirmed that this same nano-clay system produced a non-compostable material at high concentrations (100 μg/mL), as measured by its effect on lettuce seedlings. This confirms that biodegradable materials are not necessarily compostable.

Monitoring anthocyanin degradation in Hibiscus sabdariffa extracts with multi-curve resolution on spectral measurement during storage

This study aims to monitor composition changes in an anthocyanin-rich beverage during storage by the means of UV–VIS measurements associated with a multi-curve resolution procedure. Hibiscus sabdariffa extract was stored at 37 °C for 50 days and UV–VIS spectra were measured on the extract and the fractions of the extract every 5 days. MCR was carried out in two steps, first with the fraction and then the extract spectra. The results enabled the main polyphenols initially present to be identified, anthocyanins and chlorogenic acid, but also found the nature of the main degradation products: the polymers from anthocyanin condensation and scission products. In addition, comparison with HPLC analysis results showed that the MCR procedure recovered the correct shapes of the concentration profiles particularly of anthocyanin and polymer kinetics during storage. This work gives perspective for the use of a rapid and efficient technique to monitor pigment-rich beverage processing or storage.

Anti-obesity and haematological effects of Malaysia Hibiscus sabdariffa L. aqueous extract on obese Sprague Dawley rats

Background: Hibiscus sabdariffa L. (Hs; Malvaceae) is commonly known as roselle or red sorrel in English or karkadè in Arabic. It is a tropical plant native to India and Malaysia. Roselle extracts have been widely reported to have beneficial health effects. The aim of this study was to determine the effective dose of Hs aqueous extract, which is a possible reducing agent in diet-induced obese rats, and monitoring any toxicological effect.Methods: Male Sprague Dawley rats (n= 24) aged 6-7 weeks and weighing 210 ± 3.5 g (mean ± S.E.M) were used. The rats were fed a high fat diet (HFD) for 8 consecutive weeks to induce obesity. The animals were then randomly assigned to one of five groups based on the concentration of Hs aqueous extract. The normal group (NG) received a normal diet (commercial chow) throughout the study. The obese group (ObG) included obese rats administered with tap water ad libitum. Groups 1 (150 mg/kg), 2 (200 mg/kg), 3 (250 mg/kg), and 4 (300 mg/kg) were obese rats continuously fed with HFD in combination with the Hs extract for 10 weeks. The effect of Hs on some haematological and blood biochemical parameters were also evaluated.Results: Hs aqueous extract is a rich source of anthocyanins. The main compounds detected are delphinidin-3-O-sambubioside and delphinidin-3-O-sambubioside. The extract also possesses high antioxidant properties which may be caused by these anthocyanins. The anti-obesity effect of Hs aqueous extract was demonstrated by the significant reduction in the weight gain and abdominal weight (p &lt; 0.05) between treated and non-treated groups, which was dose-dependent. Overall, our study demonstrated that oral administration of Hs at doses of 150, 200, 250, and 300 mg/kg for ten weeks did not cause any toxicity effect within the obese rats.Conclusions: The results of the study implied Hs aqueous extract at 300 mg/kg is the dose which can the most weight reduction effect with no severe haematological and biochemical changes in all experimental animals.Keywords: Hibiscus sabdariffa, obese rats, roselle, aqueous extract, and body weight

Bio-friendly controllable synthesis of silver nanoparticles and their enhanced antibacterial property

Silver nanoparticles have attracted much attention in the medical field in recent years due to their excellent antibacterial properties. In this paper, size controllable silver nanoparticles were synthesized using Hibiscus sabdariffa extract (HSE) as a reductant in 10 min. The silver nanoparticles prepared using HSE as reductant have an average diameter of 15 nm, which is smaller than that of using citric acid as a reductant. Characterization results show that polyphenol compounds in HSE acts not only as reductant but also as dispersant and stabilizer. Moreover, the size of silver nanoparticles is mainly affected by pH value, reaction temperature, and kind of pH regulators. Finally, the silver nanoparticles as antibacterial component was added into cataplasm. The results showed that they have almost no cytotoxicity on 3T3 cells and excellent antimicrobial activities against Gram-positive and Gram-negative bacterium. The above research opens a feasible way for the application of silver nanoparticles in the biomedical field.

Abrogation of Hepatic Damage Induced by Anticancer Drug Methotrexate by Zobo (Hibiscus sabdariffa extract) Supplementation via Targeting Oxidative Hepatotoxicity in Rats

ABSTRACTClinical use of methotrexate (MTX) in cancer chemotherapy is limited due to its side effects, notably associated with increased oxidative stress and hepatotoxicity. Zobo is an aqueous extract of Hibiscus sabdariffa known to contain natural antioxidants. The present study investigated the hepatoprotective effect of zobo drink (ZD) on MTX-induced oxidative stress and hepatotoxicity in rats. Rats randomized to control group received distilled water orally; MTX group received intraperitoneal (ip) injection of MTX (20 mg/kg) on day 11; ZD + MTX group was administered ZD (10 ml/kg) for 14 days and was injected with MTX on day 11. Three days after MTX injection, enzyme markers of liver injury, protein profile, and bilirubin were assessed in serum. Hepatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and level of lipid peroxidation were estimated. Histopathological changes were carried out on the liver tissue. MTX induced prominent oxidative hepatotoxicity demonstrated by significant (p < .01) increases in serum liver enzymes and bilirubin, while protein profile was markedly reduced (p < .05). Hepatic activities of SOD, CAT, and GPx considerably decreased, whereas lipid peroxidation increased significantly in the MTX group compared to control. By contrast, ZD administration attenuated and restored the markers of liver injury, hepatic antioxidant enzymes, and lipid peroxidation near to control, while histopathological alterations were ameliorated compared to the MTX group. ZD affords superior protection against MTX-induced oxidative hepatotoxicity via improvement in antioxidant defense systems in rats. ZD could be a potent natural product against hepatotoxicity associated with MTX chemotherapy in cancer patients.

Fabrics dying with Hibiscus sabdariffa and Curcuma longa extracts using different mordants and mordanting methods

Extraction of plant secondary metabolites were prepared and subjected as non-toxic, biodegradable and eco-friendly natural dyes. Two groups of cotton textiles were dyed with crude extracts of Curcuma longa and Hibiscus sabdariffa separately to examine and compare the staining ability of these extracts. Fabrics where treated with three different mordanting techniques using three different mordants which were copper, ferrous sulfates and potassium dichromate. Dyed fabrics were exposed to different conditions including washing with water and detergent as well as exposing to sun light in order to test the stability of the color. As a result Curcuma longa extract had higher potentially dying ability than Hibiscus sabdariffa extract, otherwise using of Potassium dichromate as a mordant gave best color stability compared with other used mordants, while dying of fabrics by pre-mordanting method as exposing fabric samples to the mordants before dying with plant extracts showed the darker shade http://dx.doi.org/10.25130/tjps.23.2018.010

Fabrics dying with Hibiscus sabdariffa and Curcuma longa extracts using different mordants and mordanting methods

Extraction of plant secondary metabolites were prepared and subjected as non-toxic, biodegradable and eco-friendly natural dyes. Two groups of cotton textiles were dyed with crude extracts of Curcuma longa and Hibiscus sabdariffa separately to examine and compare the staining ability of these extracts. Fabrics where treated with three different mordanting techniques using three different mordants which were copper, ferrous sulfates and potassium dichromate.&#x0D; Dyed fabrics were exposed to different conditions including washing with water and detergent as well as exposing to sun light in order to test the stability of the color. As a result Curcuma longa extract had higher potentially dying ability than Hibiscus sabdariffa extract, otherwise using of Potassium dichromate as a mordant gave best color stability compared with other used mordants, while dying of fabrics by pre-mordanting method as exposing fabric samples to the mordants before dying with plant extracts showed the darker shade.