Aim: This study aimed to assess attitudes and knowledge regarding infertility among adults attending a primary healthcare facility in Turkey, along with identifying influencing factors. Methods: A descriptive cross-sectional survey was conducted among individuals aged 18-49 who admitted to a Hamidiye tranining and research hospital Family Medicine Outpatient Clinic. Participants completed the attitudes towards infertility scale (ATIS) and the Infertility Knowledge Test (IKT). Demographic data including age, gender, marital status, educational level, and reproductive history were collected. Results: A total of 237 individuals participated in the study. The median ATIS score of the participants in the study was 50, and the median IKT score was 19. ATIS scores were lower among male (p:0,023), those who were married, had children, had experienced pregnancy, had lower education level compared to other groups (p