This study describes a method to micropropagate Ariocarpus kotschoubeyanus, using coconut water and darkness conditions. The longitudinal explants from seedlings germinated in vitro were cultivated on Murashige and Skoog medium (MS) with 2 mg/l zeatin. The calli generated were transferred to medium containing 5 ml, 10 ml or 15 ml of coconut water per litre. After 84 days, five green shoots per callus were generated under photoperiod but 14 well defined shoots were produced in darkness. The shoots were cultivated on media with activated charcoal and polyethyleneglycol to reduce callus and hyperhydricity, respectively. To induce rooting the effect of the auxins indolacetic acid and indolbutyric acid (1 mg/l) were tested. The roots type II (20 to 30 mm) and type III ( > 30 mm) were observed after 120 days onto media containing auxins but not in control medium. The regenerated plants were transferred to horticultural earth, peat moss and sand (30:30:40) and the survival of the plants in soil was 80%. The plant adaptation was complete and the first flowering was observed after 1 year in greenhouse conditions. The procedure developed is an alternative to the successful propagation of A. kotschoubeyanus and reduces the risk of extinction of this species.
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