Horsfall Glycoprotein Research Articles

Tamm-Horsfall urinary glycoprotein. The subunit structure.

1. Subunit molecular weights of 76000-82000 were obtained for native and alkylated Tamm-Horsfall glycoprotein by gel filtration on Sephadex G-200 in the presence of sodium dodecyl sulphate. 2. A further estimate of the subunit molecular weight of 79000+/-4000 was obtained by disc gel electrophoresis in sodium dodecyl sulphate. 3. A minimum value of the chemical molecular weight of 79000+/-6000 was obtained from the number of N-terminal amino acids released by cyanogen bromide cleavage of the glycoprotein. 4. Similar values were obtained for the subunit molecular weight of Tamm-Horsfall glycoprotein from patients with cystic fibrosis. 5. On ultracentrifugation both in 1.0% sodium dodecyl sulphate and in 70% formic acid, Tamm-Horsfall glycoprotein sedimented as a single component, slightly faster than serum albumin. 6. On reduction of the disulphide bonds the same subunit molecular weight was obtained, which suggested that these bonds are intrachain.

The chemical composition and electron microscopic appearance of a protein derived from urinary casts

1. 1. The amino acid and carbohydrate analysis of a protein extracted from urinary casts has been performed. The protein is associated with a small amount of lipid and would appear to be indistinguishable from the Tamm and Horsfall glycoprotein of normal human urine. 2. 2. Electron microscopy of the protein extracted from casts before and after treatment with sodium dodecyl sulphate has been performed. The appearances indicate an organised linear array of subunits that is disrupted by sodium dodecyl sulphate.

Ion-Binding Properties of Electrophoretically Homogeneous Mucoproteins of Urine in Normal Subjects and in Patients with Renal Calculus Disease

No AccessJournal of Urology1 Dec 1954Ion-Binding Properties of Electrophoretically Homogeneous Mucoproteins of Urine in Normal Subjects and in Patients with Renal Calculus Disease William H. Boyce, Fred K. Garvey, and Charles M. Norfleet William H. BoyceWilliam H. Boyce More articles by this author , Fred K. GarveyFred K. Garvey More articles by this author , and Charles M. NorfleetCharles M. Norfleet More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)67711-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1954 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByYAMAGUCHI S, YACHIKU S, OKUYAMA M, TOKUMITSU M, KANEKO S and TSURUKAWA H (2018) EARLY STAGE OF UROLITHIASIS FORMATION IN EXPERIMENTAL HYPERPARATHYROIDISMJournal of Urology, VOL. 165, NO. 4, (1268-1273), Online publication date: 1-Apr-2001.Grover P and Resnick M (2018) Evidence for the Presence of Abnormal Proteins in the Urine of Recurrent Stone FormersJournal of Urology, VOL. 153, NO. 5, (1716-1721), Online publication date: 1-May-1995.Kitamura T and Pak C (2018) Tamm and Horsfall Glycoprotein Does Not Promote Spontaneous Precipitation and Crystal Growth Of Calcium Oxalate in VitroJournal of Urology, VOL. 127, NO. 5, (1024-1026), Online publication date: 1-May-1982.Grimes W (2018) A Phase Contrast Study of the Mechanisms of Renal CalcificationJournal of Urology, VOL. 78, NO. 5, (553-574), Online publication date: 1-Nov-1957.Boyce W and Garvey F (2018) The Amount and Nature of the Organic Matrix in Urinary Calculi: A ReviewJournal of Urology, VOL. 76, NO. 3, (213-227), Online publication date: 1-Sep-1956.Prince C, Scardino P and Wolan C (2018) The Effect of Temperature, Humidity and Dehydration on the Formation of Renal CalculiJournal of Urology, VOL. 75, NO. 2, (209-215), Online publication date: 1-Feb-1956.Prien E (2018) Studies in Urolithiasis: III. Physicochemical Principles in Stone Formation and PreventionJournal of Urology, VOL. 73, NO. 4, (627-652), Online publication date: 1-Apr-1955. Volume 72Issue 6December 1954Page: 1019-1031 Advertisement Copyright & Permissions© 1954 by The American Urological Association Education and Research, Inc.MetricsAuthor Information William H. Boyce More articles by this author Fred K. Garvey More articles by this author Charles M. Norfleet More articles by this author Expand All Advertisement PDF DownloadLoading ...