Horn Diameter Research Articles

Can Shunt Response in Patients with Idiopathic Normal Pressure Hydrocephalus Be Predicted from Preoperative Brain Imaging? A Retrospective Study of the Diagnostic Use of the Normal Pressure Hydrocephalus Radscale in 119 Patients.

The Normal Pressure Hydrocephalus Radscale is a combined scoring of 7 different structural imaging markers on preoperative brain CT or MR imaging in patients with idiopathic normal pressure hydrocephalus: callosal angle, Evans Index, Sylvian fissure dilation, apical sulcal narrowing, mean temporal horn diameter, periventricular WM lesions, and focal sulcal dilation. The purpose of this retrospective study was to assess the performance of the Normal Pressure Hydrocephalus Radscale in distinguishing idiopathic normal pressure hydrocephalus shunt responders from nonresponders. The preoperative MR imaging and CT scans of 119 patients with idiopathic normal pressure hydrocephalus were scored using the Normal Pressure Hydrocephalus Radscale. A summary shunt-response score assessed within 6 months from ventriculoperitoneal shunt surgery, combining the effect on cognition, gait, and urinary incontinence, was used as a reference. The difference between the mean Normal Pressure Hydrocephalus Radscale for responders and nonresponders was tested using the Student t test. The area under the curve was calculated for the Normal Pressure Hydrocephalus Radscale to assess shunt response. To ascertain reproducibility, we assessed the interobserver agreement between the 2 independent observers as intraclass correlation coefficients for the Normal Pressure Hydrocephalus Radscale for 74 MR imaging scans and 19 CT scans. Ninety-four (79%) of 119 patients were shunt responders. The mean Normal Pressure Hydrocephalus Radscale score for shunt responders was 8.35 (SD, 1.53), and for nonresponders, 7.48 (SD, 1.53) (P = .02). The area under the curve for the Normal Pressure Hydrocephalus Radscale was 0.66 (range, 0.54-0.78). The intraclass correlation coefficient for the Normal Pressure Hydrocephalus Radscale was 0.86 for MR imaging and 0.82 for CT. The Normal Pressure Hydrocephalus Radscale showed moderate discrimination for shunt response but cannot, on its own, be used for selecting patients with idiopathic normal pressure hydrocephalus for shunt surgery.

Evaluación del protocolo corto y largo de sincronización de celo en borregas inseminadas con semen congelado

The objective of the study was to evaluate the effect of short (5 days) and long (9 days) SC protocols in ewes inseminated with frozen semen on the diameter of the uterine horn and pregnancy rate. The experiment was carried out at the Carolina Experimental Center, belonging to the National University of the Altiplano, and 40 ewes be-tween Corriedale and Criollas were used; in groups of 20 for each treatment, which were synchronized with in-travaginal sponges based on Medroxyprogesterone acetate, for 9 days (long protocol) and 5 days (short protocol) and at the time of sponge removal, 350 IU of eCG were applied. To measure the diameter of the uterine horn, ultrasonography was performed at 36 h post sponge removal and at 56 h after cervical insemination with thawed semen and a subsequent ultrasonography at 35 days to confirm gestation. Quantitative data were analyzed using the statistical t-test for uterine horn diameter and Chi-square for gestation rate. The results for uterine horn diameter were 11.11±1.52 and 12.12±2.12 mm for the short and long synchronization protocol, respectively. Regarding the pregnancy rate, 21.05 % and 25 % were obtained for the short and long synchronization protocol, respectively. Therefore, we can conclude that the long progesterone treatment had better results than the short treatment both in uterine diameter and pregnancy rate, but this difference was not significant (p>0.05).

Potential Benefits of Boswellia sacra Resin on Immunity, Metabolic Status, Udder and Uterus Health, and Milk Production in Transitioning Goats

As an attempt to prevent transition-period-associated metabolic disorders and/or diseases, the potential benefits of Boswellia sacra (Bs) resin on hematology, immunity, metabolic status, udder and uterus health biomarkers, and milk production were examined in transitioning goats. From 2 weeks prepartum to 7 weeks postpartum, goats (n = 7/treatment) orally received 0 (control, nBS), 2 (BsL), or 4 (BsH) g of Bs resin/goat/day. The results showed that both Bs treatments significantly improved the activity of phagocytes. Both Bs treatments significantly decreased the concentrations of blood plasma urea, non-esterified fatty acids, hydroxybutyric acid, and interleukin-1β, whereas they increased the total antioxidant capacity compared with the nBS treatment. Both Bs treatments significantly decreased the uterine horn diameter and tended to decrease the intrauterine fluid content, indicating improved uterine involution compared with no treatment. Compared with the nBS treatment, both Bs treatments significantly decreased the milk somatic cell count. The lowest milk interleukin-1β content was observed in goats that received the BsL treatment, followed by those that received the BsH and nBS treatments. The BsL treatment significantly increased the concentration of immunoglobulin M (IgM) in the colostrum compared with the other treatments. Both Bs treatments significantly increased the daily milk yield compared with the nBS treatment but did not affect milk composition or the fat-to-protein ratio. In conclusion, supplementing lactating goats in the transition period with Bs resin at a dose of 2 g/goat/day positively affected the body lipid metabolism, udder and uterus health, colostrum IgM content, and milk yield due to its antioxidant and anti-inflammatory effects.

Variability of Normal Pressure Hydrocephalus Imaging Biomarkers with Respect to Section Plane Angulation: How Wrong a Radiologist Can Be?

Systematic analysis of angulation-related variability of idiopathic normal pressure hydrocephalus imaging biomarkers has not been published yet. Our aim was to evaluate the variability of these radiologic biomarkers with respect to imaging plane angulation. Eighty subjects (35 with clinically confirmed idiopathic normal pressure hydrocephalus and 45 age- and sex-matched healthy controls) were prospectively enrolled in a 3T brain MR imaging study. Two independent readers assessed 12 radiologic idiopathic normal pressure hydrocephalus biomarkers on sections aligned parallel or perpendicular to the bicallosal, bicommissural, hypophysis-fastigium, and brain stem vertical lines, respectively. Disproportionately enlarged subarachnoid space hydrocephalus, simplified callosal angle, frontal horn diameter, z-Evans Index, and cella media vertical width did not show significant systematic differences in any of 6 section plane combinations studied. The remaining 7 biomarkers (including the Evans Index and callosal angle) showed significant differences in up to 4 of 6 mutually compared section plane combinations. The values obtained from sections aligned with the brain stem vertical line (parallel to the posterior brain stem margin) showed the most deviating results from other section angulations. Seven of 12 idiopathic normal pressure hydrocephalus biomarkers including the frequently used Evans Index and callosal angle showed statistically significant deviations when measured on sections whose angulations differed or did not comply with the proper section definition published in the original literature. Strict adherence to the methodology of idiopathic normal pressure hydrocephalus biomarker assessment is, therefore, essential to avoid an incorrect diagnosis. Increased radiologic and clinical attention should be paid to the biomarkers showing low angulation-related variability yet high specificity for idiopathic normal pressure hydrocephalus-related morphologic changes such as the z-Evans Index, frontal horn diameter, or disproportionately enlarged subarachnoid space hydrocephalus.

Postpartum Uterine Involution and Embryonic Development Pattern in Chinese Holstein Dairy Cows.

Understanding the postpartum uterine involution pattern and embryonic development could facilitate bovine reproduction management, improve reproductive efficiency, and diagnosis of the reproductive disorder, which would contribute to the success of the dairy business. This study aimed to investigate postpartum uterine involution and embryonic developmental patterns or postconceptional marks of embryonic fetal development in Chinese Holstein dairy cows using B-mode ultrasonography. The results revealed a significant decline in the involution period with an increase of parity and age. The uterine involution period was shorter in multiparous cows when compared with cows with lower parities. Consistently, cows over 4 years old recovered faster than younger cows (2 or 3 years). Besides, the elder cows (over 4 years) had a relatively larger size of resumed cervix uteri and horns. Postpartum uterine involution pattern analysis revealed that the reproductive tract recovered very fast during the first 16 days postpartum for all the parity. Results of postconceptional marks of embryo development revealed a slow increase in diameter of the gravid uterine horn and crown-rump length (CRL) before day 60. In contrast, this increase was dramatic and rapid after the 60th day. We also established two models to estimate gestational age based on gravid uterine horn diameter or CRL. A formula was established to determine the gravid uterine horn size during postconceptional on day 30th–day 90th (r = 0.8714, P < 0.01). In addition, a significant positive correlation between CRL and gestational age (r = 0.98151, P < 0.01) was built. In conclusion, these results illustrated that parity and calving age had significant effects on uterine involution in Chinese Holstein cows. Crown-rump length and gravid uterine horn diameter are both efficient for evaluating the embryo growth. These current findings broaden the understanding of basic reproductive pattern in Chinese Holstein cows and could benefit bovine reproductive management primarily in postpartum and early pregnant cows to reduce the calving interval and avoid periparturient metabolic diseases.

Comparative biochemical profiles, utero-ovarian function, and fertility of the postpartum buffalo with and without subclinical endometritis.

In postpartum buffaloes, the process of uterine involution and changes in blood metabolic profile has not been studied in relation to development of subclinical endometritis (SCE). In this study, buffaloes (n = 100) approaching calving were identified. Weekly blood samples were collected on the day of calving up to 6weeks post-calving. The diameter of uterine horns and onset of ovarian cyclicity (corpus luteum) were recorded through ultrasonography. On the basis of polymorphonuclear cell (PMN) cell count in endometrial cytology at days 45-50 postpartum, buffaloes were divided into two groups, viz., with SCE (> 5% PMN; n = 38) and without SCE (≤ 5% PMN; n = 62). Buffaloes with SCE took longer (P < 0.05) time to complete uterine involution and had larger (P < 0.05) uterine horn diameter between 3rd and 6th weeks postpartum and lower prostaglandin F2α metabolite (PGFM) concentration on the day of calving (P < 0.05) and 1week (P < 0.001) post-calving than without SCE group. Buffaloes with SCE had lower (P < 0.001) concentration of glucose at weeks 2 and 3, higher (P < 0.001) β-hydroxybutyric acid (BHBA) at week 3, and lower serum albumin concentration throughout the sampling period (P < 0.05 to 0.001) except at 1week post-calving as compared to without SCE group. The urea concentration was significantly lower (P < 0.05 to 0.001) in buffaloes with SCE from 4weeks post-calving onwards than without SCE group. The calcium concentration was lower in buffaloes with SCE at weeks 5 (P < 0.001) and 6 (P < 0.05) postpartum, whereas the concentration of magnesium and phosphorus was uniform between the two groups. No significant (P > 0.05) difference in onset of ovarian cyclicity between the 2 groups was observed, whereas buffaloes with SCE had longer (P = 0.001) median days open (141days) than their counterpart (117days). The first service conception rate, cumulative pregnancy rate, and pregnancy rate at 150days postpartum were lower (P < 0.05) in buffaloes with SCE than without SCE group. In summary, higher BHBA and lower serum concentrations of glucose, albumin, urea, and calcium control onset of subclinical endometritis which in turn has negative impact on fertility of buffaloes.

PSIV-12 Evaluation of reproductive performance and herd longevity when heifers grazing native range are vaccinated with a lifetime regimen of a killed viral or modified live viral vaccine

Abstract Fifty-eight Angus × Hereford cross heifers born in the spring of 2015 were randomly assigned to treatments of receiving a modified-live viral (MLV) or killed viral (KV) vaccination against bovine viral diarrhea virus (BVDV) at 60-d of age to determine the effects of vaccination on fertility and herd longevity. Heifers received vaccine treatments again at weaning (205d) at re-vaccination, and a yearly booster for the duration of their time in the herd. Blood samples were collected every two weeks prior to breeding to assess puberty attainment and were subsequently subjected to ovarian ultrasonography to measure antral follicle counts (AFC) and reproductive tract score (RTS). Heifers were synchronized utilizing the 7-d CIDR-PG protocol with AI following estrus detection. Heifer AFC, uterine horn diameter, RTS, and proportion of females attaining puberty before the breeding season were similar among treatments (P ≥ 0.38). Artificial insemination pregnancy rates, final pregnancy rates, and percentage of heifers that calved in the first 21 days were similar between vaccination treatment groups (P ≥ 0.19). Body condition score through the third parity at calving and weaning were not different between vaccination types (P ≥ 0.59). Females receiving a MLV vaccination remain in the herd in a similar proportion to those receiving a KV vaccination (P = 0.86). These results indicate that different vaccination strategies can be successfully used in beef cattle grazing native range without impacting fertility, or herd longevity.

PSVII-1 A preliminary phenotypic characterization of Raramuri Criollo cattle

Abstract Raramuri Criollo (RC) are one of 33 known biotypes of heritage Criollo cattle that exist throughout the Americas today. They have been raised by the Tarahumara peoples of the Copper Canyon in Chihuahua, Mexico, for over 400 years and exhibit remarkable rusticity and adaptation to harsh grazing environments. To date, no quantitative phenotypic description of this biotype exists. Our objective was to characterize RC cattle via 26 phenotype traits to provide a preliminary biotype standard. Twenty-eight multiparous RC cows, 4 primiparous heifers, and 4 bulls were selected from a purebred herd of approximately 200 animals at the USDA-ARS Jornada Experimental Range. These 36 animals were selected by a Criollo cattle expert based on body conformation correctness. SAS 9.4 was used to determine means, SEs or frequency of measured traits. Multiparous pregnant and cycling cows averaged 366.8 ± 9.8 kg live body weight, 121.8 ± 0.9 cm withers height, horn widths of 60.3 ± 1.6 cm, horn diameters of 5.7 ± 0.1 cm, chest girths of 183.6 ± 12.0 cm, hip widths of 44.5 ± 0.5 cm, flank girths of 52.2 ± 0.9 cm, body length of 90.0 ± 1.7 cm, neck lengths of 52.0 ± 0.9 cm, and tail lengths of 83.3 ± 1.4 cm. Bulls consistently averaged higher values for all traits and weighed 618.2 ± 9.8 kg with scrotal circumferences of 36.8 ± 0.7 cm. RC cattle exhibited mostly convex nose bridges (89%), open-back horns (49%), black muzzles, hooves, and eyes (62, 69, and 81%, respectively), small ears (78%), oblique eyes (70%), high-set tail heads (86%), short hooves (95%), and short hair (78%). Our preliminary characterization of RC phenotypes will be useful in selecting RC individuals for genotypic evaluation to eventually maintain a distinct purebred registry of this Criollo biotype.

A SIW Horn Antenna without Broad Wall Loaded with Trapezoidal Air Slot

In this paper, a SIW horn antenna without broad wall loaded with trapezoidal air slot is proposed, processed, and tested. Based on the SIW horn antenna, this antenna strips off the wide wall at the horn diameter, introduces surface waves on the surface of the dielectric substrate, and improves the impedance matching between the antenna and free space. Further, trapezoidal air slots are loaded on the exposed dielectric substrate, gradually changes the dielectric constant of the dielectric substrate, so that the dielectric constant of the dielectric substrate can get closer to which of air, and the working bandwidth can be further expanded. The actual measurement results show that the working bandwidth of the antenna is 15.2-38GHz, the relative bandwidth reaches to 85.7%, and the maximum gain in the band is 9.1dBi.

Neuronavigation-assisted bedside placement of bolt external ventricular drains in the intensive care setting: a technical note.

The insertion of bolt external ventricular drains (EVD) on the intensive care unit (ICU) has enabled rapid cranial cerebrospinal fluid (CSF) diversion. However, bolt EVDs tend to be perceived as a more challenging technique, particularly when dealing with small ventricles or when there is midline shift distorting the ventricular morphology. Furthermore, if neuronavigation guidance is felt to be necessary, this usually assumes a transfer to an operating theatre. In this technical note, we describe the use of electromagnetic neuronavigation for bolt EVD insertion on the ICU and assess the protocol's feasibility and accuracy. Case series of neuronavigation-assisted bolt EVD insertion in ICU setting, using Medtronic Flat Emitter for StealthStation EM. Neuronavigation-guided bolt EVDs were placed at the bedside in n = 5 patients on ICU. Their widest frontal ventricular horn diameter in the coronal plane ranged from 11 to 20 mm. No procedural complications were encountered. Post-procedural CT confirmed the optimal placement of the EVDs. Electromagnetic neuronavigation is feasible at the ICU bedside and can assist the insertion of bolt EVDs in this setting. The preference for a bolt EVD to be inserted in ICU-as is standard practice at this unit-should not prohibit patients from benefitting from image guidance if required.

CPD article: Pre-breeding heifer examination and selection in UK beef herds

The objective of this review is to evaluate examination tools and how they can be used to assess the breeding potential of beef heifers prior to service. Reproductive tract examination techniques, pelvic measurement, and antral follicle counts (AFC) are methods that are used and could be of value to farmers where they can either identify pubescent heifers and/or those with the best reproductive potential. Reproductive tract scoring (RTS) to assess the pubertal state and therefore reproductive status of replacement heifers is explored alongside further adaptions of this method. The use of pelvic measurement is considered: first, where a small pelvic area or an abnormal shaped pelvic inlet is identified, which may result in elimination from the breeding cohort to reduce the potential risk of dystocia and poor performance; and second, since it may be advocated for use in conjunction with other methods. AFC are included since they too may help identify the reproductive potential of heifers. Evaluation of these examination tools demonstrates that the use of modified RTS methods, to include ultrasound examination to detect the absence of a corpus luteum (CL), absence of a follicle ≥ 13 mm and the uterine horn diameter as well as pelvic measurements, could add value to heifer breeding soundness examinations (HBSE) when carried out.

Evaluation of cervical and uterine size, at 4weeks postpartum, as a predictor of subsequent fertility in Jersey cattle.

Uterine and cervical size of Holstein dairy cows is reported among reasons for a decline in dairy cow fertility. Therefore, the objectives of this study were to (a) determine whether size of the cervix and uterus at 4weeks postpartum impacted subsequent fertility at first service in Jersey cattle, (b) determine whether progesterone level at 4weeks postpartum impacted cyclicity and (c) the association of the presence of corpus luteum and uterus and cervix size. Body condition scores at calving, presence of postpartum diseases, parity number and milk weights were taken from lactating Jersey dairy cows (N=147) for 28days postpartum. During the fourth week postpartum, a blood sample was obtained for progesterone concentration, and transrectal ultrasonography was performed by a high-resolution ultrasound machine to determine cervical and uterine horn diameter, as well as ovarian structures measurements. Correcting for parity number, BCS at calving, presence of diseases and milk yield, cows with a cervix >2.54±0.63cm and uterine horn >2.25±0.59cm were less likely to become pregnant at first service (p=.04 and p=.003, respectively). The cows with larger cervix had a trend to be less likely to have a corpus luteum present at the 4th week of lactation (p=.067). Cows with larger uterine horn size were less likely to have a corpus luteum present at the 4th week of lactation (p=.015). It is concluded that a larger cervix and/or uterus during the postpartum was associated negatively with fertility and cyclicity in Jersey cows.

Relationship between postpartum uterine involution and biomarkers of inflammation and oxidative stress in clinically healthy mares (Equus caballus)

To test the hypothesis that delayed/impaired uterine involution could be associated with oxinflammation, we studied the progression of the uterine involution in association with some biomarkers of inflammation and oxidative stress in clinically healthy mares (N = 26) during early postpartum. The examination of the reproductive tract was performed on Days 7 and 21 after foaling. Uterine involution was assessed considering: a) the increase of the gravid uterine horn diameter (GUHD) compared with diameter recorded before pregnancy during the previous breeding season; b) the level of endometrial edema (EE); c) the degree of accumulation of intrauterine fluid (IUFA); d) the status of the cervix (CS). Inflammation and oxidative stress were studied by measuring serum amyloid A (SAA), cortisol, DHEA, AOPP, protein carbonyl groups, malondialdheyde (MDA) and thiols in plasma on Days 7 and 21. By Day 21 after parturition, a significant improvement (P < 0.01) was observed for GUHD and EE; while IUFA increased in six animals. Plasma SAA and DHEA concentrations were higher when the clinical parameters indicated a lower degree of uterine involution. On Day 7, the cortisol/DHEA ratio was lower in animals with higher degree of EE. Plasma AOPP and MDA concentrations were significantly lower (P < 0.05) in animals with the lower GUHD. On Day 21, plasma MDA concentrations were significantly lower (P < 0.05) in animals with the lower IUFA. Our data suggest that a mild condition of inflammation and oxidative stress occur in mares with delayed/impaired uterine involution.

Effects of intra-uterine infusion of proteolytic enzymes on selected cytokine concentrations, uterine inflammation, and fertility in postpartum water buffalo cows with subclinical endometritis

The study was conducted to evaluate effects of intrauterine administration of proteolytic enzymes on endometrial inflammation and reproductive performance in postpartum water buffalo cows with subclinical endometritis (SCE). Cows (n = 38) with SCE (≥ 18 % PMN i.e.; polymorphonuclear cells) on day 21 postpartum (21 dpp), were allocated into treatment (TR; n = 19; intrauterine infusion of trypsin, chymotrypsin and papain in 20 ml normal saline on 21 dpp) and control (PC; n = 19; intrauterine administration 20 ml saline) groups. Cows without SCE (< 18 % PMN) were not treated and served as the negative control (NC; n = 30). Ultrasonography and sampling (endometrial cytology, uterine flushing, blood) were conducted on day 21 (before treatment) and 28 postpartum (28 dpp). The PMN % and uterine horn diameter were less on 28 dpp (compared with 21 dpp) in NC and TR group only. Cows with SCE had greater uterine concentrations of interleukin (IL)-1β, IL-8 and tumor necrosis factor (TNF)-α; but lesser IL-10 than NC group on 21 and 28 dpp. There were greater serum IL-1β and TNF-α concentrations on 28 dpp in cows with SCE than NC group. Uterine concentrations of IL-1β were less, whereas IL-6 was greater following enzymatic treatment. Proteolytic enzyme treatment did not result in improvement in pregnancy rate compared with the PC group; however, days to conception were less in TR compared with the other two groups. In conclusion, results indicated a reduction in endometrial inflammation and days nonpregnant after proteolytic enzyme treatment in buffalo cows with SCE.

B-mode and Doppler Ultrasonographic Assessment of Uterine Involution in Ewes Treated with Two Different Doses of ProstaglandinF2α

Background: Several studies consisted of postmortem evaluations and B-mode ultrasonography have been performed on ovine uterine involution. However, researches with Doppler ultrasonography are very limited in ewes. Doppler ultrasonography is a non-invasive method which provides information about vascular dynamics of the tissues which cannot be examined by B-mode ultrasonography. The aim of the study was to evaluate the effects of two different PGF2α doses on uterine measurements by real time B-mode ultrasonography; and on uterine artery hemodynamics by pulsed-wave Doppler analysis during postpartum uterine involution in ewes.Materials, Methods &amp; Results: The study was conducted with 30 primiparous Kiwircik ewes which lambed singleton without any complication. The ewes were randomly divided into three groups (n= 10 for each group). A single i.m. injection of 125 μg/sheep PGF2α, 75 μg/sheep PGF2α, and 1.0 mL/sheep sterile saline solution were administered to Group 1, 2, and 3, respectively. The day of parturition was considered as the first day of the study. Examinations were performed on days 1, 2, 3, 7, 14, 21 and 28. Diameters of previously gravid horn and caruncles were measured by real time B-mod ultrasonography. Presence of lochia was also noted. Uterine artery pulsatility index (PI), resistance index (RI), systolic/diastolic ratio (S/D) values were measured by pulsed-wave Doppler ultrasonography. The one-way analysis of variance and Duncan’s test were used for statistical analysis. The mean previously gravid horn diameters of ewes were 8.30 ± 0.16 cm and 1.53 ± 0.07 cm on day 1 and day 28, respectively. Previously gravid horn and caruncle diameters had a similar declining pattern in all groups as involution period proceeded. Involution was mostly completed by day 21. More than 50% reduction in uterine size was achieved in prostaglandin administered groups by day 7. Caruncles were not able to be identified after the second week postpartum. Lochia was observed for a longer period in control group. Uterine artery PI and RI showed fluctuations throughout involution period with a similar pattern among groups. S/D values progressively increased until day 14, then showed a decreasing pattern.Discussion: The effects of different PGF2α doses on uterine measurements and uterine artery hemodynamics during postpartum period were compared in ewes for the first time. The uterine size reduction in prostaglandin administered groups suggested that both PGF2α doses were effective in uterine involution. The last observation of lochia in uterine cavity were achieved by day 7 in prostaglandin administered groups, suggesting both PGF2α doses trigger uterine contractions which lead to the removal of uterine content. Automatic measurements by Doppler device were taken in order to provide uniformity and to prevent operator-based bias. The significantly higher uterine artery PI value found in Group 1 on day 1 might suggest the stimulatory effect of PGF2α administration on uterine contractility synergitically with already released endogenous PGF2α. Uterine artery PI and RI showed fluctuations throughout involution period and reached their peak values on day 14. The increasing S/D values up to day 14 indicated increasing diastolic flow, increasing resistance and decreasing blood perfusion in prostaglandin administered groups. Prostaglandin administered groups tended to show higher hemodynamic parameters throughout the study which suggested a vasoconstrictor effect of PGF2α. In conclusion, PGF2α administration on the day of parturition might have constrictor effects both on uterine artery and myometrium which leads to a reduction in uterine blood flow and a rapid decline in uterine size especially during early puerperium even with a reduced dose.

Radiological markers of idiopathic normal pressure hydrocephalus: Relative comparison of their diagnostic performance

PurposeNumerous radiological measures have been proposed as imaging biomarkers of idiopathic normal pressure hydrocephalus (iNPH), however, the number of studies systematically comparing their diagnostic values remains limited. The study objective was to compare the diagnostic performance of fifteen cross-sectional imaging iNPH biomarkers. Materials and methodsEighty subjects were prospectively enrolled in the study: 35 subjects with clinically confirmed iNPH and 45 matched healthy controls (HC). Values of linear, angular and index measurements including three newly proposed biomarkers were obtained from 3T brain MRI studies by two independent readers. Diagnostic performance of biomarkers was studied by using receiver operating characteristic (ROC) analysis and t-statistic. ResultsAll biomarkers studied were able to reliably differentiate iNPH subjects from HC (p < .001) except for cella media-to-temporal horn ratio. Z-Evans index, vertical cella media and vertical frontal horn diameters showed the highest discriminatory power between iNPH and HC groups (area under curve >0.99). Simple linear measurements of vertical (0.99) or horizontal (0.95) frontal horn diameters showed results comparable to calculated ratios, i.e. z-Evans (0.99) and Evans (0.96) indexes, respectively. ConclusionThe best diagnostic performance among fifteen radiological iNPH biomarkers was found in linear measurements referring to caudocranial alterations of the ventricular geometry, outweighing those referring to laterolateral ventricular enlargement (as e.g. commonly used Evans index). Simple linear measurements of vertical or horizontal frontal horn diameters showed comparable results to calculated, more time-consuming z-Evans or Evans indexes, respectively.

Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts.

Simple SummaryPoor sow retention is a common issue amongst piggeries that creates excessive wastage. Premature culling is largely attributed to reproductive inadequacy. Thus, it is clear the traditional methods for selecting breeding females are inefficient and early markers of reproductive success are required. The aim of this study was to examine whether circulating levels of anti-Müllerian hormone and oestradiol could be useful indicators of ovarian and uterine traits in young gilts. The findings suggest that at a young age, anti-Müllerian hormone could be used to mark the ovarian reserve. Further research into whether the two hormones could be used to mark uterine capacity is needed.Poor sow retention due to reproductive failure is a common reproductive inefficiency amongst piggeries. This shows that traditional methods of gilt selection are inadequate and a marker of reproductive success is needed. The aim of this study was to determine whether circulating levels of AMH and E2 at D80 and D160 are associated with uterine and ovarian traits at D160. Uterine weight, horn length and horn diameter were measured, and ovarian follicle counts were determined histologically. There was a negative relationship between both D80 and D160 AMH levels and D160 ovarian follicle populations. There was also a positive relationship between D80 E2 levels and uterine capacity in gilts that were pubertal at D160. The findings indicate that D80 and D160 AMH could be used to predict ovarian reserve and that D80 E2 levels may be indicative of uterine capacity in precocial gilts.

Ultrasonography of the normal reproductive tract of the female domestic cat

The objective of this study was (1) to describe the US appearance and obtain reference values for the uterus and ovaries in nongravid and gravid queens with histologically confirmed reproductive tracts without disorders, (2) to provide US measurements of the reproductive tract compared to gross macroscopic and water-bath post-OVH US measurements in nongravid queens, and (3) to describe the sonographic appearance of the female reproductive tract during the different histopathologic phases of the reproductive cycle in nongravid and gravid queens. Ninety-three queens from a “trap, neuter, return” program were included in this study. Sonographic evaluation of the reproductive tract was performed in all queens, and measurements of the corpus uteri, uterine horns, and ovaries were recorded. Following OVH, macroscopic measurements were obtained, and a water-bath US evaluation of these tissues and measurements was recorded. Samples from the corpus uteri and both the uterine horns and ovaries were collected for histopathologic examination after all measurements had been recorded. Seventy-two reproductive tracts met the inclusion criteria by having a histopathologically confirmed normal reproductive tract. Sixty-three queens were nonpregnant and 9 were pregnant. The ovaries and uterus were sonographically visible in all queens regardless of reproductive status. The ovaries were ovoid in shape, and the uterus appeared as a tubular structure with distinct wall layers (serosa and indistinct myometrium and myometrium, or serosa, myometrium, and endometrium), with variable echogenicity of the inner layers. The layering of the uterine wall, observed during the second half of pregnancy, was described. Ovarian follicles were visible in 66/72 (92%) cats. However, the CL was only visible in 40/72 (55%) cats. The reference values of the left ovarian length, right ovarian length, uterine horn diameter, and uterine body are 7.1–13.9, 7.3–13.6, 1–5.8, and 1.5–5.3 mm, respectively, in a nongravid uterus. The uterine wall thickness during pregnancy varied from 2.4 to 6.8 mm. There was a significant positive correlation between US measurements obtained in vivo and those obtained macroscopically and in a water bath post-OVH. The body weight, follicular size, sonographic visibility of the uterine wall layering, the histopathologic luteal phase, and the active/inactive status on histopathology had a significant effect on the uterine measurements (p < 0.05). It was not possible to describe the exact US features of the reproductive tract during the different histopathologic phases.In conclusion, ultrasonographic reference values for the normal female reproductive tract in cats were determined. The results of this study indicated that the ovaries and uterus were visible in cats regardless of reproductive status.

Traditional and non-traditional methods used for discrimination among Ossimi rams with different field fertility

This study aimed to differentiate among rams of different field fertility by using traditional (semen characteristics, sexual traits and testosterone level) and non-traditional [seminal plasma (SP) proteins profile, and ultrasound imaging of the testes and the accessory genital glands (AGGs)] methods. Also, the relationship between sexual traits and semen characteristics in these rams was investigated. A total of 300 ejaculates from 30 Ossimi rams [19 fertile (F), 8 subfertile (SF), and 3 infertile (IF)] were collected by electroejaculation and examined for sperm motility, viability, morphology and plasma membrane integrity. As well, sperm cell concentration and seminal index of these ejaculates were calculated. Ovine SP was recovered by centrifugation and its total protein concentration was determined by hand-held Refractometer; then SP proteins were separated on 15% (w/v) polyacrylamide gel. Horn length, horn diameter (HD), poll distance, face length, scrotal circumference (SC) and body weight of all rams were determined. All semen characteristics rather than the ejaculate volume (EV) of F rams were greater (P < 0.001) than those of SF and IF rams. On contrary, SF and IF rams had extremely (P < 0.001) prolonged stimulation time (ST) and higher % abnormal morphology (AM) compared with F rams. Moreover, F rams had greater (P < 0.01) HD and SC than SF and IF rams and these two sexual traits were correlated with semen characteristics. Ultrasound imaging of testes and AGGs revealed clear differences among the three groups of rams. Additionally, F rams had greater (P < 0.001) serum (6.51 ± 0.14 ng/mL) and SP (3.27 ± 0.69 ng/mL) testosterone concentrations than SF and IF rams. In total, 9 protein bands with molecular weight range of 6.5–175 kDa were detected in ovine SP. Protein band of 40 kDa was detected in the breeding season only and was prominent in F rams in comparison with SF and IF rams but, those of 175 and 116 kDa were obvious in IF and SF rams and faint to absent in F rams either in breeding or non-breeding season. In conclusion, however, HD and SC discriminate among rams of different field fertility but it is recommended to combine both traditional and non-traditional methods to get precise discrimination. Moreover, SP proteins were closely correlated with semen characteristics and ram fertility and that of 40 kDa might be new candidate of fertility-associated proteins in Ossimi rams.

Spontaneous ovulation in cats—Uterine findings and correlations with animal weight and age

Cats are considered induced ovulating animals but ovulations occur in the absence of mating (i.e., spontaneous ovulations). Factors that stimulate such ovulations remain largely unknown. In this study, ovaries and uterine horn segments from 89 post-pubertal queens presented for ovariectomy were evaluated morphologically and histologically. It was hypothesized that corpora lutea (CL) are present in non-pregnant cats and can be associated with cystic endometrial hyperplasia (CEH). Cats were assigned to three age groups (7–12 months, n = 32; 13–24 months, n = 26 and ≥ 24 months, n = 31) and three weight groups (2.1–2.8 kg, n = 28; > 2.8–3.3 kg, n = 32 and > 3.3 kg, n = 29). Uterine horn diameter and thickness of the endometrium and myometrium were determined. Corpora lutea were detected in 39.3% of the cats and presence did not differ between age groups. The percentage of queens with CL increased with bodyweight (2.1–2.8 kg: 14.3%; > 2.8–3.3 kg: 37.5%, > 3.3 kg: 65.5%; P < 0.01). In cats with CL, the thickness of all layers of the uterus were greater than in cats without CL (P < 0.05). Of the cats, 22.5% had CEH but there was no difference between cats without and with CL. The percentage of CEH increased with age (P < 0.001) but did not differ between weight groups. In conclusion, ovulations occurred in the absence of mating in approximately one third of all queens.