Hormonal Profile Assessment Research Articles

Gremlin-1 Level In Polycystic Ovary Syndrome And Its Clinical Correlations; A Case Control Study.

To evaluate the involvement of the level of Gremlin-1 in serum and follicular fluid in the diagnosis of polycystic ovary syndrome. The case-controlstudy was conducted at the Kafrelsheikh University Hospital, Egypt, from September 2021 to February 2022, and comprised women with polycystic ovary syndrome and healthy controls. All participants were subjected to a detailed clinical assessment, complete clinical examination and hormonal profile assessment. Gremlin1 concentrationsin plasma and follicular fluid samples were assessed by a double-antibody sandwich enzyme-linked immunosorbent assay kit. Data was analysed using SPSS 20. Of the 90 subjects, 45(50%) were patients with a mean age of 29.53±4.82 years, and 45(50%) were controls with a mean age of 30.89±6.08 (p>0.05). Mean weight, body massindex, waist circumference and waist-hip ratio were significantly higher in patients compared to controls (p<0.05). Serum and follicular fluid Gremlin-1 levels were significantly higher in the patient group (p<0.05). The best cutoff of serum Gremlin-1 in the diagnosis of polycystic ovary syndrome was ≥1.325ng/ml with area under curve 0.857,sensitivity 93.3%,specificity 68.9%, positive predictive value 75%, negative predictive value 91.2% and overall accuracy 81.1%. The best cutoff of follicular fluid Gremlin-1 in the diagnosis of polycystic ovary syndrome was ≥1.725ng/ml with area under curve 0.789,sensitivity 73.3%,specificity 68.9%, positive predictive value 70.2%, negative predictive value 72.1% and overall accuracy 71.1%. There was a strong correlation between serum and follicular Gremlin-1 levelsin polycystic ovary syndrome patients.

MP79-15 ERECTILE FUNCTION RECOVERY AMONG YOUNG MEN WITH ERECTILE DYSFUNCTION ON OaD TADALAFIL 5 MG: FINDINGS FROM A REAL-LIFE STUDY

You have accessJournal of UrologyCME1 Apr 2023MP79-15 ERECTILE FUNCTION RECOVERY AMONG YOUNG MEN WITH ERECTILE DYSFUNCTION ON OaD TADALAFIL 5 MG: FINDINGS FROM A REAL-LIFE STUDY Edoardo Pozzi, Federico Belladelli, Giuseppe Fallara, Simone Cilio, Christian Corsini, Alessandro Bertini, Massimiliano Raffo, Fausto Negri, Ludovica Cella, Margherita Fantin, Francesco Lanzaro, Luigi Candela, Paolo Capogrosso, Luca Boeri, Alessia d'Arma, Francesco Montorsi, and Andrea Salonia Edoardo PozziEdoardo Pozzi More articles by this author , Federico BelladelliFederico Belladelli More articles by this author , Giuseppe FallaraGiuseppe Fallara More articles by this author , Simone CilioSimone Cilio More articles by this author , Christian CorsiniChristian Corsini More articles by this author , Alessandro BertiniAlessandro Bertini More articles by this author , Massimiliano RaffoMassimiliano Raffo More articles by this author , Fausto NegriFausto Negri More articles by this author , Ludovica CellaLudovica Cella More articles by this author , Margherita FantinMargherita Fantin More articles by this author , Francesco LanzaroFrancesco Lanzaro More articles by this author , Luigi CandelaLuigi Candela More articles by this author , Paolo CapogrossoPaolo Capogrosso More articles by this author , Luca BoeriLuca Boeri More articles by this author , Alessia d'Arma Alessia d'Arma More articles by this author , Francesco MontorsiFrancesco Montorsi More articles by this author , and Andrea SaloniaAndrea Salonia More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003356.15AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Daily tadalafil 5 mg provides a valuable alternative to on-demand tadalafil for men who prefer spontaneous to scheduled sexual attempts. We investigated the rate of and the clinical factors associated with erectile function (EF) recovery after discontinuation of 5 mg tadalafil OaD in a cohort of young men seeking medical help for ED as primary complaint. METHODS: Data from 96 consecutive young patients (<50 yr) with ED and prescribed with 5 mg tadalafil OaD were analysed. All patients were diagnosed with psychogenic ED after a comprehensive clinical evaluation including penile duplex ultrasound and hormonal profile assessment. All completed the International IIEF at baseline. Length of treatment varied from 1 to 12 months according to clinical judgement. EF recovery was assessed after 2 weeks from treatment discontinuation and defined as IIEF-EF >21 after daily 5 mg tadalafil discontinuation. Patients without EF recovery were classified as tadalafil OaD non-responders. Cox-regression models tested the association between patients' baseline characteristics and the probability of EF recovery after treatment discontinuation. Kaplan-Meier analyses estimated the probability of EF recovery over time. RESULTS: Median (IQR) age was 39 (32-45) yr. Of all, 82 (85.4%) achieved EF recovery at treatment discontinuation, whilst 14 (14.6%) were identified as non-responders. Median treatment length was 3 (2-11) months. Most common treatment-related adverse effect was headache in 9 (9.4%) patients. Non-responders were older (43 vs. 38, p=0.03), with higher BMI (25.54 vs. 23.6, p=0.04) and depicted lower IIEF-EF scores at baseline (12 vs. 15, p=0.02) than responders. No differences in terms of comorbidities (CC≥1), smoking, alcohol consumption and regular physical exercise were detected. Kaplan Meier estimates of EF recovery at 3, 6 and 12 months, were 43% (95%CI: 41-62), 60% (95%CI: 49-69) and 75% (95%CI: 64-83), respectively (Figure 1). Younger age (HR: 0.95; 95% CI: 0.92-0.99, p=0.01) was associated with EF recovery after adjusting for baseline EF, BMI, smoking and CCI≥1. CONCLUSIONS: Almost one out of two young ED patients prescribed with tadalafil OaD achieved full EF recovery within 3 months of treatment. Younger patients have higher probability of EF recovery with daily 5 mg tadalafil therapy. Source of Funding: NA © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e1147 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Edoardo Pozzi More articles by this author Federico Belladelli More articles by this author Giuseppe Fallara More articles by this author Simone Cilio More articles by this author Christian Corsini More articles by this author Alessandro Bertini More articles by this author Massimiliano Raffo More articles by this author Fausto Negri More articles by this author Ludovica Cella More articles by this author Margherita Fantin More articles by this author Francesco Lanzaro More articles by this author Luigi Candela More articles by this author Paolo Capogrosso More articles by this author Luca Boeri More articles by this author Alessia d'Arma More articles by this author Francesco Montorsi More articles by this author Andrea Salonia More articles by this author Expand All Advertisement PDF downloadLoading ...

Assessment of hormonal profile with the genetic variation in different breeds of falcon in Iraq

This study was conducted to assessment the hormonal assay in the reproductive season of falcon in Iraq in the period duration between January to April, the animal used different breeds Saker falcon _ Falco peregrinus –and Falco tinnunculus), due to selection of breeding used in the reproduction .The objective of study was to characterize the level of testosterone hormone related with the androgen receptor gene polymorphism between deferent breeds of falcon. Blood samples were collected from metatarsal vein of males. Testosterone level recorded 9.15 ±0.74 ng, 9.23 ±0.58 ng, and 8.75 ±0.37 ng in the Falco peregrinus, gives at the result revealed significant differences between the breeds in the periods of season, initial ,middle ,and later subsequently. The molecular results revealed the polymorphism in the Falco tinnunculus. The genotype and allele frequency in the Falco peregrines and Flco Saker significantly at* (P≤0.05) in all genotypes the allele gives 4 (100%) to 0 (0%) in another one, while deferent read between genotypes in the Falco tinnunculus (P≤0.05), NS: Non-Significant, in the GG to TT, GG to AA, CC to GG, GG to AA, 2 (50%) and TT to GG at 1 (25%).

Endocrine disruption: Reproductive toxicity of glyceryl trinitrate and isosorbide mononitrate in male Wistar rats

Glyceryl trinitrate (GTN) and isosorbide mononitrate (IM) are organic nitrates which release nitric oxide upon metabolism with potential to adversely affect male reproductive function. Therefore, this study was designed to evaluate the sub-chronic effect of these organic nitrates on reproductive system in male rats. Wistar rats were separately treated with GTN and IM at 2.5, 5 and 7.5mg/kg/day by oral gavage for 45 days. At the end of treatment, serum blood samples were taken from anaesthetized rats for assessment of hormonal profile. Epididymis was removed to analyse sperm parameters. Rat testes were dissected to perform histopathological evaluation and oxidative stress biomarkers. The GTN and IM treated groups showed a significant decrease in sperm parameters (count, motility and viability) and serum testosterone in comparison to normal control group. The GTN and IM treatment also altered sperm morphology such as bent tail and head deformities as compared to control. A significant decrease in catalase activity and, increase in nitric oxide and malondialdehyde were observed in high dose drug treated groups. Moreover, a significant increase in follicle stimulating hormone and decrease in testosterone levels were evident in all drug treated groups. The level of luteinizing hormone was raised in rats treated with medium doses of drugs while it decreased at the highest dose of both drugs. Histological study showed vacuolization and degeneration of seminiferous tubules. It is concluded that GTN and IM treatment adversely affected the male reproductive function by altering sperm parameters and disrupting the reproductive hormone profile which may be attributed to the increased level of nitric oxide and oxidative stress.

WITHDRAWN: Assessment of hormonal profile and diet in relation with primary infertile females in Baghdad City − 2019

Abstract Diet, stress, exercise and smoking affect female fertility, Mediterranean healthy diet has a success rate among woman is attempting fertility. Assessment of hormonal balance and dietary pattern among primary infertile women in Baghdad City- 2019 In this study, 200 of early primary infertile women (12–18) months of marriage were interviewed and hormonal assessment was done at (4) and (21) days of the cycle, in Baghdad City-2019The study revealed that most of females were between 18 and 30 years, 30% were over 30 years, half of them were housewives, from urban areas but less than half were graduated. Mean of body mass index was (32 ± 3) that 40% was overweight and 20% was obese. Half of them had anxiety and irregular intake of supplements. About 20% of them were smokers and all of them had no exercise. Forty percent of them had increased in the level of FSH, LH; within mean average was (15 ± 2.7), (10 ± 2.8) respectively and strong correlation according to age, education, BMI while there was average correlation according to diet and supplements for FSH hormone and poor correlation for LH hormone. Fifty percent of females had increased in the level of prolactin with mean average was (24 ± 2.50 while 40% had increased in the serum level of AMH with mean average was (5 ± 2) ml/dl that both hormones had strong correlation with age, education, BMI, diet and supplements. 70% of them had decreased in the level of DHEA and D3 with mean average was (40 ± 5 ml/dl), (28 ± 5 ml/dl) respectively, with poor correlation with age and strong correlation with education, diet and supplements while according to BMI, there was strong correlation with D3 and moderate correlation with DHEA . There was normal level of testosterone and TSH with average mean was 35 ± 0.5 ml/dl and 1.7 ± 0.5 ml/dl respectively.

The impact of interferon therapy on the sexual function of hepatitis C male patients

Objective To evaluate the impact of interferon therapy on male sexual function. Patients and methods One hundred hepatitis C patients were included in the study. Fifty two patients were receiving interferon and 48 patients were not receiving interferon. All participants underwent history taking including International Index of Erectile Function (IIEF-5) score, general and local examination, assessment of hormonal profile, and pharmacopenile duplex Doppler ultrasonography. Results The IIEF-5 score of the noninterferon-treated group (18 ± 6.5) was significantly higher than the interferon-treated group (12 ± 4.5) (P = 0.022). Forty (76.9%) patients on interferon reported low sexual desire compared with 22 (45.8%) patients of noninterferon-treated group (P = 0.001). Total testosterone among interferon-treated patients (188 ± 0.32 ng/dl) was significantly lower than noninterferon-treated patients (270 ± 0.52 ng/dl) (P = 0.001). Similarly, free testosterone level among interferon-treated patients (3 ± 0.22 ng/dl) was significantly lower than noninterferon-treated group (7 ± 2.3 ng/dl) (P = 0.05). Estradiol levels among interferon-treated patients (80 ± 3.3 pg/ml) were significantly higher than noninterferon-treated patients (58 ± 2.3 pg/ml) (P = 0.01). However, prolactin level showed no significant difference between the interferon-treated (13 ± 1.3 ng/ml) and the noninterferon-treated groups (12 ± 1.5 ng/ml) (P = 0.59). Thirty eight (73%) patients on interferon showed vasculogenic erectile dysfunction compared with 32 (66.7%) patients not receiving interferon (P = 0.11). Twenty two (42.2%) patients on interferon showed veno-occlusive dysfunction which was significantly higher than noninterferon group (16.6%) (P = 0.03). Eighteen (37.5%) patients of the noninterferon group showed mixed vasculogenic erectile dysfunction compared with eight (15.4%) patients on interferon (P = 0.05). Conclusion Interferon had negative impact on libido, IIEF-5 score, and hormonal profile but did not affect penile hemodynamics.

Hormonal profile assessment in third generation inhabitants living near Semipalatinsk test site

Results of a study covering population of Maisk settlement near Semipalatinsk test site demonstrated possible thyroid diseases in 32.6% of the examinees. Predisposition to autoimmune diseases of thyroid gland (presence of antibodies to thyroid peroxidase) was revealed in 23% of the population. Increased production of stress hormone (cortisol) is characteristic for 39.4% of the females and 37.7% of the males — that is confirmed by changes in the body adaptation system of the third generation inhabitants, if ionizing radiation influence on the previous generations.

Penile Morphology among a Sample Size of Egyptian Males

Objective: To survey the different aspects of penile morphology among a sample of Egyptian males (normal, congenital curvature, etc.) and their relation to erectile dysfunction. Patients and Methods: A sample of 110 male subjects (age ranges from 20–36 years) was selected from our private centre to participate in our study. Although nearly half of them (54 subjects, 49%) had congenital penile angulation (because of physiological curvatures) of ≥ 25Eš, according to measurement on a graph, those patients were suffering from uncomfortable vaginal penetration. 14/54 of subjects (26%) were suffering from erectile dysfunction. Assessment of these patients has included; history (concentrating on the past history of trauma or surgical interventions in cases of curvatures), general and local objective examination, induction of erection using prostaglandin E1 (dosage 10–20 g), a graph was taken while the penis is erect, dynamic and basal Duplex ultrasonography examination of the penis, penile length measurement and hormonal profile assessment. Erectile dysfunction measurement was assessed using the International Index of Erectile Function-5 (IIEF-5). Results: A total of 54 subjects (49%) have suffered from congenital physiological penile curvatures of ≥ 25Eš, which made 14 of them to suffer from erectile dysfunction. Conclusion: Almost half of the selected sample had congenital penile angulation, hence, surgical correction of penile curvature correction is recommended, especially in cases suffering from erectile dysfunction or vaginal penetration discomfort.

ИНДИВИДУАЛИЗАЦИЯ ОЦЕНКИ ГОРМОНАЛЬНОГО ПРОФИЛЯ ПАЦИЕНТОК С ЭНДОКРИННЫМИ ФОРМАМИ БЕСПЛОДИЯ ПРИ ПОДГОТОВКЕ К ПРОВЕДЕНИЮ ЭКО

Questions of inspection and preparation of married couples with infertility before the application of auxiliary genesial technologies (AGT) remain extremely relevant taking into account the difficult obstetric and gynecologic status of patients belonging to programs of extracorporal fertilization, age, condition of their somatic and psychological health, and also the variety of etiological and pathogenetic factors of infertility. Objective: to determine individual algorithm of the hormonal profile assessment of genesial system for women with infertility, chronic anovulation and implications of hyper-androgyny. Materials and methods. the examined patients passed complex examination of the hormonal profile of genesial system including blood test at the 3-5th and 21-23rd day of the cycle, US monitoring of the follicular apparatus and endometrium. Conclusion. as a result of stage-by-stage inspection of patients with infertility and the adequate individually selected medicamental therapy for correction of disturbances of a menstrual cycle at the preparation for IVF, pregnancy comes in 67% of cases.

A Comparison of Bone Mineral Density in Normal Weight and Obese Adolescents with Polycystic Ovary Syndrome

Study ObjectiveTo evaluate whether there are any differences in bone mineral density (BMD) between normal weight and obese adolescents suffering from polycystic ovary syndrome (PCOS) with oligo/amenorrhea. DesignProspective cohort study. SettingAdolescent gynecology clinic in a general service hospital. ParticipantsSubjects consisted of adolescents between 16 to 18 years of age presenting with oligo/ amenorrhea with ultrasound morphology of polycystic ovaries ± evidence of hyperandrogenism over 24 months. Controls consisted of consecutive eumenorrheic patients within the same age group. InterventionsAll underwent full hormonal profile assessment, and dual energy X-ray absorptiometry and peripheral quantitative computed tomography scans. Main Outcome MeasuresAreal and volumetric BMD parameters. ResultsOf 37 adolescents with PCOS, 12 (32%) were obese with BMI ≥25, of which 9/12 (75%) were hyperandrogenic. The control group consisted of 40 normal weight eumenorrheic girls. The PCOS group overall had lower lumbar spine BMD values as compared to the controls (0.91 vs 0.97 g/ cm2, P = 0.033). The normal weight PCOS group had lower BMD at the spine (0.90 vs 0.97 g/ cm2, P = 0.027), trochanter (0.66 vs 0.71 g/ cm2, P = 0.039) as well as volumetric distal tibial core sites (268 vs 296 mg/ cm3) as compared to eumenorrheic controls, but there were no significant BMD differences between the obese PCOS group and the eumenorrheic controls. ConclusionsNormal weight PCOS adolescents with oligo/amenorrhea have marginally lower BMD values than controls, but obese PCOS adolescents have BMD values compatible with eumenorrheic adolescents.

Our Experience of Hormone Replacement Therapy To Optimize Aesthetic Surgery Results

Study Objective: To assess the efficacy of hormone replacement therapy (HRT) in comprehensive rehabilitation after aesthetic surgery and develop an algorithm of clinical and hormonal monitoring.Design: Examination and follow-up of 92 patients after face and neck lift.Setting: Gynecology Department.Patients: Ninety-two women (ages 37-69 yrs) examined before face and neck lift and followed up for 18 months postoperatively.Measurements and Main Results: Preoperative clinical laboratory examination were complemented by assessment of hormonal profile, aging markers, oncology markers, bone resorption markers, lipoproteid fractions, coagulogram and also pelvic ultrasonography, oncocolpocytology, x-ray densitometry and mammography. Postoperative assessment by rehabilitation doctor included four parameters: extent of tissue edema, complications, sensitivity recovery and subjective facial assessment by patients. Each parameter was scored 1 to 5; the first two parameters had negative scores. The total score of 4 parameters was recorded for each patient at 1, 3 and 9 months. Three groups of patients were followed up: Group 1 (n=63) receiving HRT perioperatively; Group 2 (n=21) without HRT due to contraindications; Group 3 (n=8) not requiring HRT according to preoperative findings (controls). At month 1 parameters of all groups had similar scores (–7.3 to –7.9). At month 3 Groups 1 and 3 had higher scores than Group 2. At month 9 the difference in score between Groups 1 and 2 was 2.8. Group 1 had fewer subjective complaints and rehabilitation measures between months 3 and 9, while Group 2 complained of skin tightness and dryness, paresthesia and loss of sensitivity.Conclusion: The use of HRT in conjunction with aesthetic surgery is pathogenetically grounded. It reduces the extent of rehabilitation measures postoperatively and elevates subjective assessment of the results. Hormones are chosen individually, which reduces side effects. HRT is not limited to estrogen only. Adequate HRT is an important component of rehabilitation that helps maintain the achieved aesthetic results. Study Objective: To assess the efficacy of hormone replacement therapy (HRT) in comprehensive rehabilitation after aesthetic surgery and develop an algorithm of clinical and hormonal monitoring. Design: Examination and follow-up of 92 patients after face and neck lift. Setting: Gynecology Department. Patients: Ninety-two women (ages 37-69 yrs) examined before face and neck lift and followed up for 18 months postoperatively. Measurements and Main Results: Preoperative clinical laboratory examination were complemented by assessment of hormonal profile, aging markers, oncology markers, bone resorption markers, lipoproteid fractions, coagulogram and also pelvic ultrasonography, oncocolpocytology, x-ray densitometry and mammography. Postoperative assessment by rehabilitation doctor included four parameters: extent of tissue edema, complications, sensitivity recovery and subjective facial assessment by patients. Each parameter was scored 1 to 5; the first two parameters had negative scores. The total score of 4 parameters was recorded for each patient at 1, 3 and 9 months. Three groups of patients were followed up: Group 1 (n=63) receiving HRT perioperatively; Group 2 (n=21) without HRT due to contraindications; Group 3 (n=8) not requiring HRT according to preoperative findings (controls). At month 1 parameters of all groups had similar scores (–7.3 to –7.9). At month 3 Groups 1 and 3 had higher scores than Group 2. At month 9 the difference in score between Groups 1 and 2 was 2.8. Group 1 had fewer subjective complaints and rehabilitation measures between months 3 and 9, while Group 2 complained of skin tightness and dryness, paresthesia and loss of sensitivity. Conclusion: The use of HRT in conjunction with aesthetic surgery is pathogenetically grounded. It reduces the extent of rehabilitation measures postoperatively and elevates subjective assessment of the results. Hormones are chosen individually, which reduces side effects. HRT is not limited to estrogen only. Adequate HRT is an important component of rehabilitation that helps maintain the achieved aesthetic results.

A comparison of bone mineral density in oligomenorrhoeic adolescents with polycystic ovaries and normal ovaries

We evaluated whether the presence of polycystic ovaries in adolescent girls as a cause of oligomenorrhoea and amenorrhoea would pose any protective effect against osteoporosis or low bone mineral density (BMD) compared with girls having similar menstrual dysfunction but normal ovaries. A cross-sectional observational study was done in consecutive girls, aged between 16 and 19 years, presenting to the adolescent gynaecology clinic with oligomenorrhoea or amenorrhoea. All patients underwent full hormonal profile assessment, pelvic ultrasound for ovarian morphology, bio-impedance estimation of body fat, and dual-energy X-ray absorptiometry and quantitative peripheral computed tomography scans to determine BMD in axial and appendicular skeletal sites. Polycystic ovaries were diagnosed according to ultrasound morphology. These were then compared with an age-matched eumenorrhoeic control group that had undergone the same evaluation. Of 45 patients with oligomenorrhoea or amenorrhoea, 14 (31%) were diagnosed to have polycystic ovaries, while the other 31 had normal ovaries. The control group consisted of 45 age-matched eumenorrhoeic girls. The group with normal ovaries had lower BMD at the lumbar spine and hip, as well as lower total tibial volumetric BMD, than the eumenorrhoeic controls, but there were no significant differences between the group with polycystic ovaries and eumenorrhoeic controls. We conclude that adolescents with oligomenorrhoea and amenorrhoea with normal ovaries had lower BMD than eumenorrhoeic ones, but those with polycystic ovaries had BMD values comparable to those of eumenorrhoeic controls despite their menstrual dysfunction.