Homogenization Speed Research Articles

Preparation of chitosan microspheres by emulsion crosslinking method for biomedical application

The emulsion crosslinking method using glutaraldehyde as a crosslinking agent was exploited to prepare chitosan microspheres. Effects of preparation factors such as homogenization speed, oil/emulsifier ratio (O/E), oil/water ratio (O/W), crosslinker concentration on the structure of products were investigated. Structure and morphology of the obtained products were determined by Fourier transform infrared spectroscopy (FT-IR), Trasmission Optical Microscope systems (TOM), and Dynamic Laser Scattering analysis (DLS). The results indicated that the obtained products were spherical in shape. The average diameter of chitosan microspheres decreased with the increase of homogenization speed, O/W ratio, crosslinker concentration or decrease of O/E ratio. When homogenization speed is 6000 rpm, O/E =1.5, O/W=15, and molar ratio of chitosan to glutaraldehyde R=1, the average diameter of the chitosan microspheres was about 2.40 μm with a narrow size distribution. Chitosan microspheres are able to be used as a polymer matrix for targeted drug delivery systems due to their pH-responsive property.

Extraction of Ethylparaben by emulsion liquid membrane: Statistical analysis of operating parameters

Ethylparaben, alkyl esters of p-hydroxybenzoic acid, is an endocrine disruptor that interfere glandular activity and hormone production and even with considerable elimination of them using conventional treatment methods, it is still recognized in river water samples at high concentrations. In this study, Emulsion Liquid Membrane (ELM) consisted of trioctylamine (TOA) as carrier, n-heptane as diluent, Span 80 as surfactant and Na2CO3 as the stripping solution was used for the removal of ethylparaben (EP) from aqueous solution. Studies were conducted to analyse the effect of various operating parameters. Effect of factors like carrier concentration (C), stripping phase concentration (N), speed of homogenizer (H), surfactant concentration (S) on the extraction of EP were evaluated with Taguchi's method. Statistical analysis of variance (ANOVA) was applied to analyze the effect of each parameter and stripping phase concentration (0.5 N Na2CO3) followed by speed of homogenizer (13500 rpm) were found to be the most influencing parameters. Photomicrographs of emulsions and droplet size analyzer were used to study the impact of speed of homogenizer on emulsion droplet size and size distribution. Dispersion destabilization of the emulsion was detected by Turbiscan. Atomic force microscopy and Fourier transform infrared spectroscopy were used to analyse dimensions, shape of emulsion droplet and also to confirm bonding and the presence of different functional groups. Experimental design for multivariable optimization by Taguchi had also been implemented. Using a stable emulsion at optimum condition ∼90% extraction of EP was achieved.

Homogenization Effect on Nanostructure and Conductivity of Polyaniline Nanofibre Synthesis by Mini-Emulsion Polymerization Technique

Nanofibre polyaniline (n-PANI) was synthesized by mini-emulsion polymerization technique between aniline monomer and ammonium persulfate as an oxidant using homogenizer. The synthesis was performed by optimizing mixing speed from 10,000 to 30,000 rpm and time reaction between 0.5 to 24 hours at fixed monomer to oxidant molar ratio 4:1. An attempt has been made to investigate on how the speed of homogenizer affects the size and conductivity of n-PANI. The formation of n-PANI chain was confirmed by Fourier transform infrared spectroscopy (FTIR). The X-ray diffraction (XRD) spectra revealed PANI crystalline nature. Hall effect measurement used indicated that the electrical conductivity of n-PANI is increased with homogenizer speed from 5.2 to 17.5 Scm-1. The morphological properties of n-PANI performed by scanning electron microscopy (SEM) show the decreasing size of n-PANI from 50-60 nm to 20-30 nm with the increment homogenizer speed. This study indicated the optimum speed parameter of homogenizer play a role in reducing the nanostructured size and thus, increasing the electrical conductivity of n-PANI.

Demulsification of Liquid Membrane Emulsion using High Voltage Glass Coated Electrode in Batch System

Demulsification is one of the key processes in emulsion liquid membrane application. This study involved the effect of electrical field on demulsification of water in oil using batch high voltage demulsifier system. This technique widely used because of its advantages of easy manipulation of applied field direction and strength, offers cost–effective separation and minimal environmental impact combined with mechanical simplicity. Influence of various values of frequency (400–1500 Hz) and voltage was studied experimentally using Alternate Current (AC) High Voltage Demulsifier with insulated electrode. The emulsion consists of kerosene as organic phase, sulfuric acid as internal phase and span 80 (3 and 5 w/v %) as a surfactant. The effect of emulsion preparation such as homogenizer speed, internal phase and surfactant concentrations on the stability of water–in–oil emulsion was also investigated. The results showed that the attractive forces between the water droplets under an electrical field increase by raising the applied electrical field strength. Meanwhile, the time required for the emulsion to separate and coalescence under electrical field increased when the stability of emulsion increased.

Assessment of formulation parameters needed for successful vitamin C entrapped polycaprolactone nanoparticles

ABSTRACTVitamin C (Vit.C)-entrapped polycaprolactone (PCL) nanoparticles (Vit.C–PCNs) were prepared by encapsulation of Vit.C into PCL-based nanoparticles (PCNs) which were prepared using double emulsion method with two steps. First, the inner aqueous phase (W1) was added to dichloromethane solution containing PCL with homogenization to form primary emulsion (W1/O) which was emulsified with the outer aqueous phase (W2) containing polyvinyl alcohol as stabilizer to attain the double emulsion (W1/O/W2). Versatile parameters were investigated to reach to the most successful formulation for Vit.C–PCNs, such as time, effect of speed of homogenization on drug encapsulation efficiency, etc.

Stability of Palm Oil-based Emulsion Liquid Membrane for Succinic Acid Extraction from Aqueous Solution

Emulsion liquid membrane (ELM) process has high potential in the separation of succinic from the fermentation broth. However, the major drawback of this technology is the stability of emulsion globules during the extraction process and the chemical involved in the liquid membrane formulation. This study investigate the stability of ELM using a greener formulation containing Amberlite LA-2 as a carrier, Span 80 and Tween 80 as a surfactant, palm oil as a diluent and sodium carbonate (Na2CO3) as an aqueous stripping agent. The emulsion stability was evaluated by observing the water-oil separation of the emulsion and microscopic image of emulsion droplets count and size. Several operating parameters including the organic to internal ratio, homogenizer speed, homogenizing time, and surfactant concentration, and surfactant blend were investigated. The results show the most stable water-in-oil emulsion was observed at 3:1 organic to internal ratio; 7000rpm homogenizer speed; 5 minute emulsification time; 3% (w/v) surfactant at HLB 8. Besides, the extraction study shows 70% of the succinic acid was extracted at 0.01M Na2CO3, 1:3 treat ratio, and 0.7M Amberlite in palm oil at optimum primary emulsion stability conditions. This indicates the potential of using palm oil based ELM for the extraction of succinic acid.

Stabilizing O/W Emulsions by Soy Protein Concentrate + Maltodextrin and Optimizing the Process by Using Response Surface Methodology

Stability is one of the key quality parameters of emulsion systems, which goes a long way in predicting the shelf life of emulsion products. In this study, the effect of emulsifier (soy protein concentrate (SPC) + maltodextrin (MDX)), dispersed phase (lycopene in oil solution) and homogenizer speed on emulsion stability were investigated and optimized using response surface methodology (RSM). Independent variables were lycopene content (10-20%, w/w), SPC+ MDX as emulsifier and surfactant (30-40%, w/w) and the homogenizer speed (14000-18000 rpm). Responses were droplet size, viscosity and creaming index as stability indicators of the emulsions. According to RSM analysis and models, optimized variables showed a good fit to quadratic equations for droplet size and viscosity with correlation coefficients (R2) of 0.9571 and 0.9826, respectively. After model simplification with backward stepwise solution, the R2 values for droplet size and viscosity decreased slightly to 0.9504 and 0.9826, respectively. Creaming values were fitted properly with linear model, and R2 was 0.8030. Graphical optimization methods were adapted for preparing the best lycopene emulsifying conditions and were predicted to be: homogenizer speed of 18000 rpm; lycopene content of 20% w/w, and SPC+MDX concentration of 36.95% w/w.

Peyer'e patch targeting of Isradipine loaded Solid Lipid Nanoparticles: It's cellular uptake study

Isradipine (ISR), BCS Class II antihypertensive drug undergoes extensive first pass metabolism (FPM) with low bioavailability (15–24%). FPM may be avoided by formulating ISR loaded Solid Lipid Nanoparticles (SLN). Lipidic ISR-SLN get absorbed through M-Cells of peyer's patches. Preformulation study was performed. Dynasan 114 was selected as lipid. Poloxamer 407 was selected as surfactant. ISR-SLN was prepared using High Speed Homogenization-Ultrasonication method, optimized by 32 full factorial design. ISR-SLN was lyophilized. (1:5 = solid: cryoprotactant). Process parameters like homogenization speed, time and sonication cycle were also optimized. In-Vitro % Cummulative drug release(%CDR) study of ISR-SLN (dispersion & lyophilized) was performed using dialysis bag method in 0.1 N HCl+2% tween 80 for 2 h (obtained 10% drug release) than in phosphate buffer Ph 7.4 + 2% tween 80 (Aprx. 100% drug release after 130hrs, followed higuchi model). Ex-Vivo %CDR was performed using chicken duodenum in same condition as In-Vitro. Characterization was done using zeta potential (−21.5 mv), TEM(Spherical shape and smooth surface), MPS(204 nm), %EE (90.85 ± 3.55%), XRD, DSC, FT-IR. Confocal Laser Scanning Microscopy of Rodamine B labeled ISR-SLN was performed to study uptake of ISR-SLN through M-Cells, Peyer's Patches. Stability study of 45 days shows the ISR-SLN is stable. ISR-SLN give controlled release, permeates M-Cell of Peyer's patch and are stable.

Optimization of processing parameters for the development of Ficus religiosa L. extract loaded solid lipid nanoparticles using central composite design and evaluation of antidiabetic efficacy

The aim of the present work was to optimize processing parameters for the preparation of Ficus religiosa L. extract loaded solid lipid nanoparticles (SLN) using central composite design. Optimization was carried out using four factors such as homogenization speed, homogenization time, sonication time and sonication intensity and three responses studied were particle size, polydispersity index (PDI) and zeta potential to obtain a SLN batch with lesser particle size, optimum PDI and higher zeta potential. Further, optimized batch was characterized for entrapment efficiency, surface morphology, in-vitro release and kinetics, fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), powder Xray diffraction (PXRD) and stability over 6 months. From the results, it was observed that increase in homogenization speed and time decreased particle size and PDI with increase in zeta potential value. Increase in sonication time up to 5 min decreased particle size and further increase had no effect on particle size. Sonication intensity had very little effect. DSC and PXRD showed reduced crystallinity of extract in SLN form. Also, the optimized batch had stability over 6 months. Further, SLN significantly reduced diabetes induced higher levels of blood glucose and increased diabetes induced lower level of plasma insulin.

Loteprednol Etabonate Nanoparticles: Optimization via Box-Behnken Design Response Surface Methodology and Physicochemical Characterization

Abstract: The objective of the present work was to prepare and optimize the loteprednoletabonate (LE) loaded poly (D,L-lactide co-glycolide) (PLGA) polymer based nanoparticle carrier. The review on recent patents (US9006241, US20130224302A1, US2012/0028947A1) assisted in the selection of drug and polymer for designing nanoparticles for ocular delivery applications. The nanoparticles were prepared by solvent evaporation followed by high speed homogenization. Biodegradable polymer PLGA (50:50) grade was utilized to develop various formulations with different drug:polymer ratio. A Box-Behnken design with 33 factorial design was selected for the present study and 17 runs were carried out in totality. The influence of various process variables (viz., polymer concentration, homogenization speed and sonication time) on the characteristics of nanoparticles including the in vitro drug release profile were studied. The nanoparticulate formulations were evaluated for mean spherical diameter, polydispersity index (PDI), zeta potential, surface morphology, drug entrapment and in-vitro drug release profile. The entrapment efficiency, drug loading and mean particle size were found to be 96.31±1.68 %, 35.46±0.35 % and 167.6±2.1 nm respectively. The investigated process and formulation variables were found to have significant effect on the particle size, drug loading (DL), entrapment efficiency (EE), and in vitro drug release profile. A biphasic in vitro drug release profile was apparent from the optimized nanoparticles (NPs) for 24 hours.

Optimization of Cilnidipine Nanosuspension Using a Center Composite Design

The purpose of this investigation was to increase the solubility and dissolution rate of Cilnidipine by the preparation of nanosuspensions with solvent antisolvent method at the laboratory scale. Drug solution of Cilnidipine in acetone this mixture was added to stabilizer solution under continuous homogenization. Central composite design was employed to study the effect of the independent variables on the dependent The relationship between the dependent and independent variables was further Elucidated using multiple liner regression analysis (MLRA) and contour plots. Various process and formulation parameters were screened like homogenization speed, solvent to antisolvent ratio, homogenization time, type of stabilizer, concentration of drug and concentration of stabilizer. Physical stability can be enhancing physical stability of this colloidal system; nanosuspensions were freeze dried using D-mannitol. Seven different stabilizers were tried. Among them Poloxamer 407, Poloxamer 188, PVA and Tween 80 yielded nanosuspension in range of 90 to 350 nm. Freeze dried nanosuspensions were filled in capsules to make a deliverable dosage form and almost 100% drug dissolved in 5 minutes. The outcome of this study reveals the immense potential of nanosuspensions for delivery of Cilnidipine by improving its solubility and dissolution rate. These results show that the preparation of Cilnidipine-loaded nanosuspensions significantly improved the in vitro dissolution rate, thus possibly enhancing the fast onset of therapeutic drug effect.

The impact of homogeniser speed, dispersing aggregate size and centrifugation on particle size analyses of pork as a measure of myofibrillar fragmentation

Particle size analysis has been proposed as a measure of myofibrillar fragmentation resulting from post-mortem proteolysis in meat. The aim of this study was to examine the effect of homogenisation speed, dispersing aggregate size and centrifugation on particle size characteristics of pork loin. Particle size characteristics were significantly (P≤0.023) greater for samples aged 2 than 8d for all but the 80 and 90% quantiles. Differentiation with ageing was only achieved when homogenised at 11,000rpm using the smaller dispersing aggregate (9 vs 13mm rotor diameters). Centrifugation had no effect on particle size characteristics. Significant correlations with MFI (r=−0.40 to −0.81, P<0.001) and shear force in meat aged at 3.7°C (r=0.36–0.47; P<0.001) were observed. Weak or inconsistent correlations with shear force suggest adoption of particle size analyses as a method of tenderness classification unlikely. Rather, value lies in the detailed profiles of particle size distributions with meat ageing.

Development of mask-cream technology with herbal substances for the androgenic alopecia treatment

Aim. The aim of the study was to develop a rational technology of mask-cream containing Sabal palm extract and Sophora japonica tincture applied for prevention and tratment of androgenic alopecia. Methods. Pharmaco-technological, physico-chemical, rheological and microscopy methods were used. Results. Using microscopy studies aimed to determine the dispersion of the oil phase particles of the mask-cream it was found that the largest percentage of emulsion drops of up to 4 μm, in particular 74.4 %, was in the sample produced by reverse emulsion method. The given sample was characterized by the structural viscosity value at 20 oС and 20 rpm – 9060 mPa·Sec, which was close to the carrier base viscosity. The increase of homogenization speed and period sufficiently reduced the average size of the dispersed phase only to a certain point, therefore, optimal parameters for the cream-mask homogenization were chosen – 2000 rpm, within 20 minutes. The active substances (Sabal palm extract, Sophora japonica tincture) and the excipients (preservatives, thickener, flavor) were put into the base gradually, concerning their physical and chemical properties. Conclusion. Technological parameters of the mask-cream preparation using emulsion base were determined: reverse emulsion method, homogenization parameters – 20 minutes at 2000 rpm. Technological scheme for production of the mask-cream containing Sabal palm extract and Sophora japonica tincture was developed

Development of chitosan based extended-release antioxidant films by control of fabrication variables

In this study, mechanical, optical and permeability to water vapor of chitosan containing α-tocopherol film as the function of preparation conditions including concentration of emulsifier and speed of homogenization have investigated. In addition, the effect of above mentioned variables and presence of ethanol as co-surfactant on the release rate of α-tocopherol from chitosan film to fatty food simulant (ethanol 95%) were investigated. Fourier transform infrared spectroscopy and differential scanning calorimetry were employed to analyze the structural and thermal properties of the films. Results showed that the incorporation of α-tocopherol and preparation conditions affected the physical and mechanical properties of the chitosan films. Obtained results indicated that increasing the concentration of Tween 80 increased the release rate of α-tocopherol in the most studied films. Increasing the stirring speed of homogenization and the presence of ethanol considerably decreased the release rate of α-tocopherol at the most film samples. The lowest amount of released antioxidant was 8.6–10% of total incorporated α-tocopherol at the first stages and is obtained when ethanol used during preparation of film forming solution. Our results indicated that the release rate of α-tocopherol could be controlled by changing the stirring speed of homogenization and especially ethanol presence, considerably.

Development of long-circulating docetaxel loaded poly (3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles: Optimization, pharmacokinetic, cytotoxicity and in vivo assessments

Long-circulating nanoparticles (NPs) are promising drug delivery vehicles which target solid tumors via enhanced permeation and retention effect. Plackett–Burman (PBD) and Box–Behnken (BBD) designs were adopted to study the effects of factors viz. polymer concentration, surfactant concentration, homogenizer speed, homogenization time and ultrasonication time on responses. A graphical and numerical optimization technique was used to obtain predicted value of the response. The drug entrapment efficiency was approximately 39±0.85%. The particle size of the nanoparticles was found to be 260±2.85nm, while the zeta potential was −18±2.12mV, indicating more stable particles. SEM, TEM, and AFM were used for characterization of surface morphology and the physicochemical characters of NPs. A pharmacokinetic evaluation carried out intravenous administration in healthy Charles Foster rats displayed enhanced systemic bioavailability and plasma drug concentration. The in vivo-in silico assessment by GastroPlus™ showed good prediction accuracy and presented best-fit model. Nanoparticles were also studied for stability testing and were found to be stable concerning their drug content and physical characters. In vitro cytotoxicity was assessed using MCF-7 for percentage inhibition of human breast cancer cell line. Anticancer studies of optimized NPs showed a significant increase in efficacy as observed by relative tumor volume up to 30 days.

Novel polyglycerol-dioleate based cubosomal dispersion with tailored physical characteristics for controlled delivery of ondansetron

In this study different amphiphilic lipids viz. glycerylmonooleate (GMO), Glycerylmonolinoleate (GML), Plurol® (polyglycerol 3-dioleate), Soya phosphatidylcholine (Soya PC), Dipalmitoyl-phosphatidylcholine (DPPC) and Dipalmitoyl- phosphatidylglycerol (DPPG) were screened for their ability to form cubosomes and the formed particles were optimized for controlled delivery using ondansetron as a model drug. Polarized optical microscopy and high resolution transmission electron microscopy were performed to investigate the morphology of the prepared particles. A full factorial study was developed to optimize formulation composition and process conditions. Atomic force microscopy and in vitro drug release studies were done on selected formulae. Results showed that GMO, Plurol and GML were able to form cubosomes while DPPC was able to form spherical nanoparticles. There was a negative correlation between homogenization speed and PS, PDI and EE%. Furthermore, there was a significant positive correlation between Lipid: Polymer ratio and PS as well as PDI values, while there was a slight negative correlation between it and EE% in all nanoparticles except for those based on DPPC, which showed significant increase in EE% upon increase in the lipid:polymer ratio. Additionally, cumulative percent release of ondansetron from the particles shows that their design was efficiently tailored throughout this study to afford sustained drug release with diffusion pattern. T80 reached 19.3h in case of Plurol. Further confirmation studies on cubic structure formation were done on selected GMO and Plurol-based nanoparticles using scanning electron microscopy and x-ray powder diffraction. Results showed that Plurol was able to form cubic nanostrucures resembling those formed by GMO. Finally, Plurol (polyglyceroldioleate) showed to be a promising amphiphilic lipid that is capable of forming cubic nanostructures like GMO opening a new era for polyglycerols to take a step as a self assembling lipid resembling the unsaturated monoglycerols (UMGs).

Improved release of triamcinolone acetonide from medicated soft contact lenses loaded with drug nanosuspensions

Drug nanosuspensions (NSs) show a significant potential to improve loading and release properties of the poorly water soluble drug triamcinolone acetonide (TA) from poly(hydroxyethyl methacrylate) (pHEMA) soft contact lenses. In this work, TA NSs were developed by a controlled precipitation method using a fractional factorial Plackett–Burmann design. Poloxamer 407 (PL) and polyvinyl alcohol (PVA) as stabilizing agents were selected. NSs were characterized in terms of their drug content, particle size and morphology. Results indicate that all studied factors, except homogenization speed and sonication, have significant influence on the drug incorporation yield into NSs. Drug nanoparticles showed an interesting size that may be suitable for their incorporation into topical ocular drug delivery systems, as hydrogels.pHEMA hydrogels and daily-wear Hilafilcon B commercial contact lenses (SCLs) were employed to study TA loading capacity and drug release properties using NSs as loading system. Hydrogels have been synthesised by copolymerization of 2-hydroxyethyl methacrylate (HEMA) with methacrylic acid (MA) in accordance with a previous work (García-Millán et al., 2015). Both synthesised hydrogels and SCLs were characterized in terms of their mechanical and physical properties and TA loading and release properties. Selected TA NS was further characterized by studying its physical-chemical stability during the loading process.Results show that the use of TA NSs as loading medium significantly increases drug loading capacity and release of soft contact lenses in comparison with drug saturated solution. Synthesised pHEMA hydrogels and SCLs lenses have good properties as ophthalmic drug delivery systems, but SCLs load higher quantities of drug and release TA in shorter time periods than synthesised pHEMA hydrogel.

Influence of processing factors on the stability of model mayonnaise with whole egg during long-term storage.

Mayonnaise-like oil-in-water emulsions with different stabilities-evaluated from the degree of macroscopic defects, e.g., syneresis-were prepared by different formulations and processing conditions (egg yolk weight, homogenizer speed, and vegetable oil temperature). Emulsions prepared with lower egg yolk content were destabilized for shorter periods. The long-term stability of emulsions was weakly related to initial properties, e.g., oil droplet distribution and protein coverage at the interface. Protein aggregation between oil droplets was observed and would be responsible for the instability of emulsions exhibited by the appearance defects. SDS-PAGE results for adsorbed and unadsorbed proteins at the O/W interface suggested that predominant constituents adsorbed onto the interface were egg white proteins as compared with egg yolk components when the amount of added egg yolk was low. In present condition, egg white proteins adsorbed at the O/W interface could be a bridge of neighboring oil droplets thereby causing flocculation in emulsions.

Properties of gelatin-based films incorporated with chitosan-coated microparticles charged with rutin

The aim of this study was development an active film based on gelatin incorporated with antioxidant, rutin carried into microparticles. The complexation between oppositely charged lecithin and chitosan was applied to prepare the chitosan-coated microparticles. The generated microparticles had an average size of 520±4nm and a span of 0.3 were formulated by a rotor-stator homogenize at the homogenization speed 10,000rpm. Composite films were prepared by incorporating chitosan-coated microparticles, at various concentrations (0.05, 0.1, 0.5, or 1% (based on the weight of the gelatin powder)) in the gelatin-based films. For the prepared films, the results showed that obtained physicochemical, water vapor barrier, and mechanical were compared with native gelatin film with a slight decrease for chitosan concentration higher than 0.5%. The microstructure studies done by scanning electron microscopes, revealed different micropores embedded with oil resulting from the incorporation of the microparticles into the gelatin matrix. Moreover, the calorimetric results were comparable to those of gelatin control film with Tg value 45°C and increased crystallinity percentage with increasing incorporation of microparticles. This original concept of composite biodegradable films may thus be a good alternative to incorporate liposoluble active compounds to design an active packaging with good properties.

Optimization of Manufacturing Conditions for Improving Storage Stability of Coffee-Supplemented Milk Beverage Using Response Surface Methodology.

This study aimed at optimizing the manufacturing conditions of a milk beverage supplemented with coffee, and monitoring its physicochemical and sensory properties during storage. Raw milk, skim milk powder, coffee extract, and emulsifiers were used to manufacture the beverage. Two sucrose fatty acid esters, F110 and F160, were identified as suitable emulsifiers. The optimum conditions for the beverage manufacture, which can satisfy two conditions at the same time, determined by response surface methodology (RSM), were 5,000 rpm primary homogenization speed and 0.207% sucrose fatty acid emulsifier addition. The particle size and zeta-potential of the beverage under the optimum condition were 190.1 nm and - 25.94±0.06 mV, respectively. In comparison study between F110 added group (GF110) and F160 added group (GF160) during storage, all samples maintained its pH around 6.6 to 6.7, and there was no significant difference (p<0.05). In addition, GF110 showed significantly higher zeta-potential than GF160 (p<0.05). The particle size of GF110 and GF160 were approximately 190.1 and 223.1 nm, respectively at initial. However, size distribution of the GF160 tended to increase during storage. Moreover, increase of the particle size in GF160 was observed in microphotographs of it during storage. The L* values gradually decreased within all groups, whereas the a* and b* values did not show significant variations (p<0.05). Compared with GF160, bitterness, floating cream, and rancid flavor were more pronounced in the GF110. Based on the result obtained from the present study, it appears that the sucrose fatty acid ester F110 is more suitable emulsifier when it comes to manufacturing this beverage than the F160, and also contributes to extending product shelf-life.