Homogeneous Line Research Articles

Stretch-Induced Ordering of Prochiral Dimethyl Sulfoxide in Anisotropic Hydrogels Analysed by 1H and 2H Nuclear Magnetic Resonance.

Nuclear spins in small molecules dissolved in stretched hydrogels typically have population-averaged residual interactions. The nuclear magnetic resonance (NMR) spectra of these systems often show additional peaks and splittings compared with free solutions. Residual dipolar couplings (RDCs) and quadrupolar couplings (RQCs) are observed for guest1H or2H nuclear spins, respectively. Dimethyl sulfoxide (DMSO) is an exquisitely sensitive probe of such biologically relevant environments since it is prochiral and becomes effectively chiral when embedded in anisotropic gelatin-based hydrogels. Measured1H RDCs and2H RQCs were used to estimate bond order parametersover a wide range of stretching extents.At the largest extent of stretching, the2H splittings were-73.0 and-9.4 Hz, similar to those found for guest molecules in liquid crystals. Inhomogeneous line broadening of the2H resonances was related to the size of the RQC due to a spatial distribution of RQCs, which was revealed using a one-dimensional slice selective imaging experiment along the stretching direction.1H NMR spectra exhibited homogeneous line broadening, with resonance integrals that indicated concealed multiplet structure. Understanding molecular bond ordering in mechanically oriented environments provides a conceptual framework for investigating more complex systems including zeolites and those foundin vivo.

Exciton-Phonon Coupling in Single ZnCdSe-Dot/CdS-Rod Nanocrystals with Engineered Band Gaps from Type-II to Type-I.

Exciton-phonon coupling limits the homogeneous emission line width of nanocrystals. Hence, a full understanding of this is crucial. In this work, we statistically investigate exciton-phonon coupling by performing single-particle spectroscopy on colloidal Zn1-x Cd x Se/CdS and CdSe/CdS dot-in-rod nanocrystals at cryogenic temperatures (T ≈ 10 K). In situ cation exchange enables us to analyze different band alignments and, thereby, different charge-carrier distributions. We find that the relative intensities of the longitudinal optical S- and Se-type phonon replicas correlate with the charge-carrier distribution. Our experimental findings are complemented with quantum mechanical calculations within the effective mass approximation that hint at the relevance of surface charges.

Hua operators on homogeneous line bundles over non-tube type bounded symmetric domains

Let Ω=G/K be a bounded symmetric domain of non-compact type. In this paper the image of the Poisson transform on the degenerate principal series representations of G attached to the Shilov boundary of Ω is considered. We characterize the images in terms of the third-order Hua operators Uν and Wν. When Ω is the exceptional domain of type V, we give the explicit formulas for the operators Uν and Wν.

Superradiation in a Medium Consisting of Two Ultra-Thin Layers, Considering the Influence of Homogeneous and Inhomogeneous Spectral Line Broadening

Superradiation in a Medium Consisting of Two Ultra-Thin Layers, Considering the Influence of Homogeneous and Inhomogeneous Spectral Line Broadening

Bulge-Free and Homogeneous Metal Line Jet Printing with StarJet Technology.

The technology to jet print metal lines with precise shape fidelity on diverse substrates is gaining higher interest across multiple research fields. It finds applications in additively manufactured flexible electronics, environmentally friendly and sustainable electronics, sensor devices for medical applications, and fabricating electrodes for solar cells. This paper provides an experimental investigation to deepen insights into the non-contact printing of solder lines using StarJet technology, eliminating the need for surface activation, substrate heating, curing, or post-processing. Moreover, it employs bulk metal instead of conventional inks or pastes, leading to cost-effective production and enhanced conductivity. The effect of molten metal temperature, substrate temperature, standoff distance, and printing velocity was investigated on polymer foils (i.e., PET sheets). Robust printing parameters were derived to print uniform, bulge-free, bulk metal lines suitable for additive manufacturing applications. The applicability of the derived parameters was extended to 3D-printed PLA, TPU, PA-GF, and PETG substrates having a much higher surface roughness. Additionally, a high aspect ratio of approx. 16:1 wall structure has been demonstrated by printing multiple metal lines on top of each other. While challenges persist, this study contributes to advancing additively manufactured electronic devices, highlighting the capabilities of StarJet metal jet-printing technology.

Nano(bio)Materials Do Not Affect Macrophage Phenotype—A Study Conducted by the REFINE Project

Macrophages are well known for their involvement in the biocompatibility, as well as biodistribution, of nano(bio)materials. Although there are a number of rodent cell lines, they may not fully recapitulate primary cell responses, particularly those of human cells. Isolation of tissue-resident macrophages from humans is difficult and may result in insufficient cells with which to determine the possible interaction with nano(bio)materials. Isolation of primary human monocytes and differentiation to monocyte-derived macrophages may provide a useful tool with which to further study these interactions. To that end, we developed a standard operating procedure for this differentiation, as part of the Regulatory Science Framework for Nano(bio)material-based Medical Products and Devices (REFINE) project, and used it to measure the secretion of bioactive molecules from M1 and M2 differentiated monocytes in response to model nano(bio)materials, following an initial assessment of pyrogenic contamination, which may confound potential observations. The SOP was deployed in two partner institutions with broadly similar results. The work presented here shows the utility of this assay but highlights the relevance of donor variability in responses to nano(bio)materials. Whilst donor variability can provide some logistical challenges to the application of such assays, this variability is much closer to the heterogeneous cells that are present in vivo, compared to homogeneous non-human cell lines.

STUDY OF ACUTE AND SUB-ACUTE TOXICITY OF A MEDICATION BASED ON SPIDER VENOM EXTRACT

Homeopathy is a regulatory therapy. The effectiveness of homeopathic therapy does not depend on the dose of the drug, but on the frequency of its repetitions. The composition of homeopathic preparations includes a wide range of natural and synthetic active substances: minerals; chemical substances; substances of plant origin; microorganisms, including fungi, bacteria, viruses, and plant parasites; substances of animal origin; and human materials, which may include tissues, secretions, hormones, and genetically homogeneous cell lines. One such substance is an extract of spider venom (Tarantul cubensis D6), which remains active in pharmaceutical preparation for a long time. The drug is selectively absorbed in inflamed tissues, differentiating them from healthy cells and suppressing inflammation. The drug is also effective in necrotic and proliferative processes. It is used for the treatment of panaritium in the initial stage, skin ulcers, necrobacillosis, and necrosis of the soft tissues of the animal. Any materials of animal or human origin are potentially dangerous for human and animal health, as they may contain toxic or pathogenic substances. Therefore, the study of the acute and subacute toxicity of spider venom extract is one of the stages of establishing the toxicological parameters of the drug. The use of the drug in a therapeutic dose caused an increase in animal body weight, hemoglobin concentration, number of leukocytes, erythrocytes, hematocrit value, average erythrocyte volume (MCV), average hemoglobin content in erythrocytes (МСН), average concentration of hemoglobin in erythrocytes (МСНС), number of platelets, and AST activity against the background of a slight decrease in total protein content, creatinine, urea, and ALT activity. The use of the drug in a 10-fold therapeutic dose caused an increase in animal body weight, creatinine, urea, ALT, and AST activity against the background of a decrease in the concentration of hemoglobin, the number of leukocytes and erythrocytes, the value of hematocrit, the average volume of erythrocytes (MCV), the average content of hemoglobin in erythrocytes (МСН), the average concentration of hemoglobin in erythrocytes (МСНС), the number of platelets, and the content of total protein.

The Perspective of Metallic Access Networks Based on MELT Parameter Measurements on Homogeneous Symmetric Lines

In this paper, attention is focused on the perspective of metallic access networks utilizing homogeneous symmetric lines as a transmission medium. This miscellaneous environment has a strong influence on transmitted Digital Subscriber Line (DSL) signals and hence it is necessary to know its specific parameters in the given case. Fast and precise measurements on homogeneous symmetric lines are still essential, meaningful and imperative for many reasons: solving interference problems, designing advanced signal processing techniques and realizing appropriate simulation models. There are various ways of testing measurements with meaningful differences. In this contribution, we are focusing on the importance of measurements on homogeneous symmetric lines by means of metallic ended line testing. Subsequently, possibilities for new evaluation techniques for Metallic Ended Line Testing (MELT) parameters and for analyzing of DSL service provision are presented. Additionally, the realization of a mobile application functionality for facilitating the jobs of technicians is introduced, together with the possible availability of homogeneous symmetric lines for DSL service provision.

Multi-Terminal Fault Locator for Homogeneous Transmission Lines Using Extended Proportions

Multi-Terminal Fault Locator for Homogeneous Transmission Lines Using Extended Proportions

Влияние липополисахаридов грамотрицательных бактерий различных таксономических групп на морфогенетическую активность каллусов мягкой пшеницы

In vitro tissue culture is actively used in modern plant breeding for genetic transformation, effective selection, rapid production and propagation of genetically homogeneous lines. For many crops, the low yield of regenerated plants is limiting for the use of calluses in the breeding process. Some bacterial lipopolysaccharides are able to increase the morphogenetic activity of plant calluses, increasing the efficiency of in vitro technology. In this work, we investigated the effect of lipopolysaccharides (LPS) from the rhizobacterial strains Ensifer adhaerens T1Ks14, Ochrobactrum quorumnocens T1Kr02, and Pseudomonas chlororaphis K3 on the processes of callusogenesis, the formation of morphogenic calluses and regenerant plants of common wheat (Triticum aestivum L.) of a low-morphogenic line with the Rht-B1a dwarfing gene, obtained on the basis of the cultivar Saratovskaya 29. It was found that LPS preparations at a concentration of 10 μg/ml had different effects on wheat tissue cultures. The yield of calluses from explants obtained from immature embryos significantly increased by 2.5% when exposed to LPS of the O. quorumnocens strain T1Kr02 and decreased by 4% in the variant with LPS from P. chlororaphis K3. The yield of morphogenic calluses increased under the influence of LPS from E. adhaerens T1Ks14 (+57%) and P. chlororaphis K3 (+41%). LPS strain O. quorumnocens T1Kr02 had a negative effect on the yield of regenerated plants, while the other two LPS preparations did not differ from the control in this parameter. As a result, the effectiveness of using the LPS preparations from explants to regenerants was significantly higher only in the variant of the E. adhaerens strain T1Ks14 (+64%). The data obtained indicate significant differences in the response of plant tissue cultures to LPS of various rhizosphere bacteria.

Transient Response of Homogenous and Nonhomogenous Bernoulli Production Lines

The transient response of production systems is of significant importance especially if present advancements in Digital Twinning technology are taken into account. While the steady-state response enables long-term strategic decision making, the transient response enables more detailed simulation concerning aspects like production losses and preventive maintenance. This is especially relevant if nonhomogenous aspects of production systems are taken into account. An analytical and approximative solution to the problem of the transient response of homogenous and nonhomogenous Bernoulli production systems is developed in this paper based on the eigendecomposition of transition matrices, the eigenvalue problem, and the finite-state method. In particular, sub-resonant and resonant nonhomogeneous production lines are introduced for the first time. Also, the most significant key performance indicators are developed as functions of the time elapsed from the first cycle. Finally, the relationship between the number of eigenvalues and the accuracy of the results is inspected by employing a sensitivity analysis. The presented theoretical framework was employed in the case of a wood processing facility to present the potential application of the theory in the case of long- and short-term management of production systems.

Polarization-dependent intensity ratios in double resonance spectroscopy.

Double Resonance is a powerful spectroscopic method that unambiguously assigns the rigorous quantum numbers of one state of a transition. However, there is often ambiguity as to the branch (ΔJ) of that transition. Spectroscopists have resolved this ambiguity by using the dependence of the double resonance intensity on the relative polarization directions of pump and probe radiation. However, published theoretical predictions for this ratio are based upon a weak (i.e., non-saturating) field approximation. This paper presents theoretical predictions for these intensity ratios for cases where the pump field is strongly saturating in the two limits of transitions dominated by homogeneous or of inhomogeneous broadening. Saturation reduces but does not eliminate the magnitude of the polarization effect (driving the intensity ratio closer to unity) even with strong pump saturation. For the case of an inhomogeneously broadened line, such as when Doppler broadened linewidth dominates over the power-broadened homogeneous line width, a large fraction of the low pump power polarization anisotropy remains. This paper reports predicted polarization ratios for both linear and circular pump and probe field polarizations. The present predictions are compared with experimental measurements on CH4 ground state → ν3 → 3ν3 transitions recently reported by de Oliveira et al.63 and these are in better agreement than with the weak field predictions.

The first case of gynogenesis induced by cold- and heat-shock treatment in Thamnaconus modestus

Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. The objective of this study was to induce meiotic gynogenesis in Thamnaconus modestus through the application of cold- or heat-shock treatment, with the ultimate goal of establishing a foundational population to enhance breeding outcomes. Firstly, we obtained the optimal dosage of ultraviolet irradiation (55 mJ/cm2) required to render the sperm genome inactive while still allowing egg activation without any contribution of genetic material to the progeny. After fertilization with the inactivated sperm, diploidization was achieved through either cold-shock (12 °C for 45 min) or heat-shock (38 °C for 1 min), which suppressed the extrusion of the second polar body. Gynogenetic offspring were confirmed through genotyping using 12 microsatellite markers with parental specificity. The heterozygosity of meiotic gynogenesis group decreased by 35.14% when analyzed using SNP markers through whole genome resequencing technique, compared with the wild populations. Overall, the meiotic gynogenetic families were successfully induced, and this is the first protocol described for the induction of gynogenesis in T. modestus, which establishing a complete method of meiotic gynogenesis induction. The procedure described herein may be used to produce a clonal line of fish or for the breeding of improved varieties of T. modestus and other related species.

Plant regeneration through somatic embryogenesis in cell suspensions of Cenchrus ciliaris L.

BackgroundThis study aims to establish cell suspension culture, its maintenance and induction of somatic embryogenesis, and in vitro plant regeneration in Cenchrus ciliaris L. Suspension cultures are relatively homogenous cell lines facilitating uniform access to nutrition. These are ideal sources of competent cells for genetic transformation.ResultsCallus was initiated by culturing immature inflorescences of Cenchrus ciliaris cv. IGFRI-3108 on Murashige and Skoog (MS) medium containing 3 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg l−1 6-benzylaminopurine (BAP) with 30 g l−1 sucrose. Cell suspension cultures were established in liquid MS medium using an inoculum size of 10 g l−1. These were maintained to achieve embryogenic cell/regeneration competent cultures. Growth curve analysis and a subculture interval of 20 days were determined to harvest cells at the end of the exponential phase. The cell doubling time was found to be 11 days. Somatic embryogenesis was accomplished in MS medium containing 1 mg l−1 2,4-D, 1 mg l−1 BAP along with growth adjuvants, 300 mg l−1 casein hydrolysate, 400 mg l−1 glutamine and 300 mg l−1 proline. The highest number (16 ± 3.78/per inoculum) of shoots regenerated on this medium. The elongation and rooting of shoots were recorded on basal MS and ½ MS media, respectively. Rooted plants were successfully transferred to pots containing a Soilrite and cocopeat mixture in a 3:1 proportion for 3–4 weeks and later successfully acclimatized in the greenhouse with a 60% survival rate. The genetic fidelity of 12 regenerated plants was analysed using RAPD primers that were genetically identical to the mother plant.ConclusionCell suspension culture-based in vitro plant regeneration of C. ciliaris involved the establishment, maintenance and progression of somatic embryogenesis during shoot and root development. The inherent limitation of callus-mediated in vitro plant regeneration reducing the regeneration potential due to the aging of the calli has been overcome.

Functional and molecular characterization of the Atlantic salmon gill epithelium cell line ASG-10; a tool for in vitro gill research.

Fish gills are not only the respiratory organ, but also essential for ion-regulation, acid-base control, detoxification, waste excretion and host defense. Multifactorial gill diseases are common in farmed Atlantic salmon, and still poorly understood. Understanding gill pathophysiology is of paramount importance, but the sacrifice of large numbers of experimental animals for this purpose should be avoided. Therefore, in vitro models, such as cell lines, are urgently required to replace fish trials. An Atlantic salmon gill epithelial cell line, ASG-10, was established at the Norwegian Veterinary institute in 2018. This cell line forms a monolayer expressing cytokeratin, e-cadherin and desmosomes, hallmarks of a functional epithelial barrier. To determine the value of ASG-10 for comparative studies of gill functions, the characterization of ASG-10 was taken one step further by performing functional assays and comparing the cell proteome and transcriptome with those of gills from juvenile freshwater Atlantic salmon. The ASG-10 cell line appear to be a homogenous cell line consisting of epithelial cells, which express tight junction proteins. We demonstrated that ASG-10 forms a barrier, both alone and in co-culture with the Atlantic salmon gill fibroblast cell line ASG-13. ASG-10 cells can phagocytose and express several ATP-binding cassette transport proteins. Additionally, ASG-10 expresses genes involved in biotransformation of xenobiotics and immune responses. Taken together, this study provides an overview of functions that can be studied using ASG-10, which will be an important contribution to in vitro gill epithelial research of Atlantic salmon.

A multi-scale assessment of shoreline changes at an undeveloped beach in southern Brazil

This research aims to investigate shoreline variability at an undeveloped beach in southern Brazil on interannual and millennial scales. The long-term coastal evolution was investigated by GPR, whereas the interannual scale shoreline changes were analyzed by the Digital Shoreline Analysis System (DSAS). Simultaneously, a supervised Maximum Likelihood image classification was applied to the dune systems to differentiate vegetation from sand. The results show that the coastline in the Praia das Cabras region is retreating at different scales. The stratigraphic record in GPR shows that the coast has a retrogradational stacking pattern behavior in geological time. At the same time, the results obtained from the digital shoreline analysis revealed that the coast has suffered short-term erosion in the analyzed period, and this is attributed to natural causes. Another point is the higher values of erosion in the extreme parts of the study area, which are related to the presence of washouts. There is also a differentiation in the stage of the dunes and vegetation between the northern and southern parts of the stretch. The northern area is more densely vegetated and has a continuous foredune ridge with incipient dunes forming a ramp, whereas in the southern part, the vegetation is more dispersed, with interrupted dunes forming nebkhas. Between 2019 and 2023 there was a recovery of the dune field, by filling in the blowouts and minimizing the indentations, forming a more homogeneous foredune line.

Parallel line identification for line-implicit-solvers

Line-implicit preconditioners are well known in computational fluid dynamics (CFD) solvers and are an essential component to handle meshes with cells of very high aspect ratio (> 1000:1). Such anisotropic cells are commonly used to resolve steep gradients in the boundary layer of a turbulent flow with high Reynolds number. To date, this technique has rarely been used to solve other partial differential equations. We show that the advantages of such preconditioners do not depend on the partial differential equation or discretization used, but also apply to other problems like a node-based mesh deformation with linear elasticity on such meshes. We show the influence of the selection of these lines, and present a new algorithm for identifying lines for line-implicit preconditioners. This new algorithm makes better use of parallel processors and leads to more homogeneous lines. Finally, we see that using the same line-implicit preconditioner, but the new line identification algorithm, even leads to faster convergence for the mesh deformation problem based on linear elasticity.

Plasmon Energy Transfer Driven by Electrochemical Tuning of Methylene Blue on Single Gold Nanorods.

Plasmonic photocatalysis has attracted interest for its potential to generate energy-efficient reactions, but ultrafast internal conversion limits efficient plasmon-based chemistry. Resonance energy transfer (RET) to surface adsorbates offers a way to outcompete internal conversion pathways and also eliminate the need for sacrificial counter-reactions. Herein, we demonstrate RET between methylene blue (MB) and gold nanorods (AuNRs) using in situ single-particle spectroelectrochemistry. During electrochemically driven reversible redox reactions between MB and leucomethylene blue (LMB), we show that the homogeneous line width is broadened when spectral overlap between AuNR scattering and absorption of MB is maximized, indicating RET. Additionally, electrochemical oxidative oligomerization of MB allowed additional dipole coupling to generate RET at lower energies. Time-dependent density functional theory-based simulated absorption provided theoretical insight into the optical properties, as MB molecules were electrochemically oligomerized. Our findings show a mechanism for driving efficient plasmon-assisted processes by RET through the change in the chemical states of surface adsorbates.

Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (Pisum sativum)

We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (PIC) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding histories. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over the years and accessions, respectively. The mean thousand seed weight was 152.05 g. More than 10 accessions had a protein content above 28%, showing that the collection has potential as breeding nursery for high-protein pea cultivars.

Label-free tumor cells classification using deep learning and high-content imaging

Many studies have shown that cellular morphology can be used to distinguish spiked-in tumor cells in blood sample background. However, most validation experiments included only homogeneous cell lines and inadequately captured the broad morphological heterogeneity of cancer cells. Furthermore, normal, non-blood cells could be erroneously classified as cancer because their morphology differ from blood cells. Here, we constructed a dataset of microscopic images of organoid-derived cancer and normal cell with diverse morphology and developed a proof-of-concept deep learning model that can distinguish cancer cells from normal cells within an unlabeled microscopy image. In total, more than 75,000 organoid-drived cells from 3 cholangiocarcinoma patients were collected. The model achieved an area under the receiver operating characteristics curve (AUROC) of 0.78 and can generalize to cell images from an unseen patient. These resources serve as a foundation for an automated, robust platform for circulating tumor cell detection.