Methyl alcohol is an alcohol derivative that is widely used in the industry and is easy to obtain. Methyl alcohol intoxication is a serious condition. Especially in recent days, the cases of methyl alcohol intoxication, which has caused the death of many people, have again focused attention on this intoxication in Turkey. Use of homemade drinks containing methyl alcohol and fake alcohol can be shown as the reason for this. Forensic cases (mostly postmortem) with positive methyl alcohol results were analyzed. The presence of psychoactive, legal, and illegal abuse potential substances was also evaluated in the samples of the positive cases. The toxicological analysis results of biological samples (postmortem and antemortem) belonging to forensic cases that came to the Forensic Medicine Institute Izmir Group Presidency, Chemistry Department between 2017–2020 were evaluated. Methyl alcohol and its metabolites are routinely analyzed by headspace-gas chromatography (HS-GC) and headspace-gas chromatography/mass spectrometry (HS-GC/MS) in biological samples (postmortem-ante mortem). In addition to methyl alcohol, the analysis of illegal, legal, and medical substances in blood and urine samples is performed by liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). The analysis results were obtained from the National Judicial Network Project (UYAP) and then analyzed statistically. In the samples of the positive cases, the presence of psychoactive, legal, and illegal abuse potential substances was also evaluated. In total, 42,564 cases were screened and methyl alcohol was detected in 0.6% ( n = 247) of these cases. The mean age of methyl alcohol-positive cases was 53.43 ± 10.66 (min: 25; max: 76), 95.1% of which were male. In total, there are 205 postmortem, 4 antemortem, and 38 unreachable cases with methyl alcohol detected. Although there was a significant increase between the years, the positive rate increased significantly in 2020 compared to the previous year (22 times more). The most common cause of death was methyl alcohol intoxication. In the majority of cases (16.6%), the methyl alcohol level was in the range of 150–300 mg/dL, followed by the concentration range of 301–499 mg/dL (12.6%). In the evaluated cases, besides the results of methyl alcohol, the presence of medical, medically abused legal, abused legal, and abused illegal substances in blood and urine were also examined. The medical and medically abused legal substance has mostly been detected in blood and urine. The high taxes on alcoholic beverages and the prohibitions that came into effect during the pandemic period caused an increase in illegal alcohol production. In our country, it has been prohibited to sell ethyl alcohol and methanol online since 2020. Despite this ban, the increase in the detection of methyl alcohol in 2020 is remarkable to our findings. As can be seen from the results of our study, increasing methyl alcohol use increases the responsibility of the people working in forensic and clinical laboratories. Especially during the pandemic period considering the increase in individual news, reveals the necessity of scientific data instead of popular data on the use of methyl alcohol. With this study, it is predicted that providing data to the field of forensic toxicology will both shed light on toxicological analyzes and benefit lawmakers for strategies developed to prevent the use of methyl alcohol.