Compost Research Articles

BIMBINGAN TEKNIS PEMBUATAN PUPUK KOMPOS PADA MASYARAKATPERI-URBAN DI DESA ABEKO KECAMATAN RANOMEETO BARAT KABUPATEN KONAWE SELATAN

Thematic KKN activities carried out in Abeko Village, Ranomeeto Barat District, South Konawe Regency, focused on technical guidance on making compost for peri-urban communities. This program aims to improve community skills and knowledge in processing organic waste into environmentally friendly and economical compost. This activity is in line with efforts to support government programs in realizing sustainable agriculture and reducing dependence on chemical fertilizers that can damage the soil in the long term. The evaluation results showed that this training was able to improve the understanding and skills of participants, as evidenced by their success in making compost piles that met standards. In addition, participants were also able to identify organic materials available in the surrounding environment, such as plant residues and livestock manure, and understand how to maintain temperature and humidity during the fermentation process.

Treating Tropical Soils with Composted Sewage Sludge Reduces the Mineral Fertilizer Requirements in Sugarcane Production

Conventional mineral fertilization (CMF) is a common practice in infertile sugarcane-cultivated tropical soils, increasing production costs and environmental concerns. Combining CMF with composted sewage sludge (CSS) could be a sustainable strategy. We aim to evaluate changes in soil chemical properties, macro- and micronutrient concentrations in the soil surface (Ap1; 0–25 cm) and subsurface (Ap2; 25–50 cm) horizons, after CSS application with or without CMF in sugarcane cultivation (first and second ratoon cane). Eleven treatments, featured by CSS increase rates and mixed with CMF at different concentrations, were tested in the first ratoon; during the second, the CSS residual effect was evaluated. Applying CSS in sugarcane-cultivated soils, improved the following: (i) soil organic matter, pH, the sum of bases, cation-exchange capacity, and base saturation; (ii) overall nutrient concentrations (P, K, Ca, Mg, B, Cu, and Zn). The treatments showing the best performances were those with 5.0 Mg ha−1 of CSS. Composted sewage sludge has the potential for use as an organic natural fertilizer reducing the need for CMF. When applied in infertile tropical soils, additional positive effects can be achieved, such as decreasing production costs and providing socio-economic benefits.

Variations in the level of available phosphorus with changes in the status of water-soluble organic matter derived from different organic materials in soil

The combined application of organic material and phosphorus fertilizer is an effective method to enhance phosphorus use efficiency for plant growth. This is partly because the presence of water-soluble organic matter (WSOM) derived from different organic materials can enhance the level of available phosphorus in the soil; however, it is poorly understood how this level varies with changes in the WSOM status (i.e., decomposed, dissolved, and retained) in the soil depending on WSOM types. This study aimed to (i) understand how changes in the WSOM status enhances the available phosphorus level in the soil, and (ii) determine the WSOM type that contributes to such enhancement. The incubation test showed that fractions of 73%–92% and 8%–27% of WSOM-derived organic carbon were retained and dissolved, respectively, at the beginning of incubation, while 31%–45% was decomposed during the incubation period. The WSOM derived from cattle manure compost (CM) and sewage sludge compost (SSC) that was initially retained was maintained until the late stage of the incubation test, whereas that derived from hydrothermal decomposed liquid fertilizer (HDLF) was rapidly desorbed during the first 14 days of the incubation period. The available phosphorus level was higher under the combined application of CM- and SSC-derived WSOM than under the single phosphorus application throughout the incubation period, while it was high only during the first 3 days of incubation under the application of HDLF-derived WSOM. The amounts of retained organic carbon at each sampling point during the incubation period compared to those at the beginning were positively and linearly correlated to the available phosphorus levels that were enhanced by the WSOM present in the soil. This study for the first time provides quantitative experimental evidence that 1) the longer the WSOM continues to be retained, the higher the amount of available phosphorus remaining in the soil, and 2) the available phosphorus level decreases with WSOM sorption or decomposition. Furthermore, it was shown that highly humified WSOM has a great potential for the maintenance of higher available phosphorus levels. This study provides the insight that a combined application of highly humified organic materials with a chemical fertilizer is necessary for not only cost effective but also sustainable fertilization design.

Straw additive enhances manure compost quality by promoting diverse aerobic bacteria and unitary thermophilic fungi

Shortening the initial activation time and extending the duration of the thermophilic phase are key to improving compost product quality in cold-climate regions. This study set up three treatments that cattle manure (CM), manure with rice straw (MR), and manure with maize straw (MM) in the field with ambient temperature ranging from –6 to 7 ℃. Compared with traditional manure composting, composting with straw performed more effectively, and the effect of the addition of maize straw surpassed that of the addition of rice straw. Straw additives markedly increased the compost pile temperature and extended the thermophilic phase duration. In addition, straw addition improved compost product maturity, as indicated by the humic-like substance content, absorbance ratio, and germination index. To further illustrate this result, the microbial community structure during the composting process was studied. During the thermophilic phase, straw additives, especially maize straw, improved the formation of a diverse aerobic bacterial community and a unitary thermophilic fungal community, and promoted a stronger relationship between the bacterial and fungal communities, as revealed by co-inertia analysis. The abundance of functional genes indicated that straw addition increased the activities of organic carbon degradation and transformation. This study demonstrated the necessity of enhancing the interaction between thermophilic–aerobic bacteria and thermophilic fungi to improve compost product quality.

Impact of biogas adoption on household energy use and livelihood improvement in Kenya: an overview on a roadmap toward sustainability

Purpose This study assesses the impact of biogas adoption on household energy use and livelihood improvement. Also, this study aims to clarify the benefits of biogas adoption as a pathway to sustainable household energy. Design/methodology/approach The study explored the benefits of adopting biogas uptake. Fourteen sustainability indicators were identified, validated and categorized. Findings Adopting biogas technology provides numerous benefits, including better air quality, reduced deforestation and lower greenhouse gas emissions. Households can replace up to 4.5 tons of wood fuel, cutting CO2 emissions by around 6.75 tons annually. This shift saves approximately US$25 (Ksh.3223) monthly and frees up 45.5 h per week, enabling families to pursue additional income-generating activities. Biogas also produces digestate, a natural fertilizer that improves soil fertility, water retention and reduces erosion while minimizing the need for chemical fertilizers. Integrating biogas in livestock housing reduces odors, pathogens and methane emissions. Research limitations/implications The findings demonstrate numerous social, economic and environmental implications of biogas use. Social implications Health benefits include lower exposure to smoke and particulate matter, particularly benefiting women and children by reducing respiratory issues, improving lighting and enhancing educational opportunities. Biogas further improves hygiene, promotes cleanliness, strengthens energy security and alleviates energy poverty. In addition, the construction, operation and maintenance of biogas systems create jobs, and the use of digestate enhances agricultural productivity. Originality/value This study provides a unique and thorough analysis of the benefits of biogas, offering valuable insights and outlining a sustainable approach.

Hydroponic: A Study of Space Farming

This paper demonstrates the possibility of supporting human life in space, by growing fresh vegetables and producing them through simulated growing conditions. Supplying fresh vegetables to space stations and manned space missions is complicated and prohibitively expensive. Growing plants can be tough in space because of zero gravity, unavailability of soil, fertilizers, etc. Hydroponic is an advanced technology for growing plants without using natural resources (i.e. soil, air, natural fertilizers, etc.). It is a combination with greenhouses, it is advanced technology and highly intensive. The hydroponic technology takes place inside the enclosures to control air, temperature, and humidity using a vapor pressure deficit (vpd) controller, nutrient flow, and water flow controller. A major concern is the micro-gravity, which causes roots to grow differently than those from earth farming. In microgravity and hypo-gravity conditions, space farming utilizes a variety of methods like hydroponics, aeroponics, etc. This paper focuses on the hydroponics design, structure, operations, technique, substrate suitable for the plants, pH values, water level, and controllers required for hydroponic technology. The main objective of the paper is to build a preliminary design that is completely automatic that is robust and foolproof and find the definitive solution to the problems in the substrate and multi-spectrum lighting aroused due to gravity and vacuum conditions. The fully automated systems help to the reduction of labor and provide good healthy food to astronauts.

A case study of consecutive pregnancies in a woman with liver cirrhosis and portal hypertension

Pregnancy in women with liver cirrhosis is a high-risk one that requires multidisciplinary care. Variceal bleeding occurs in up to half of women having associated portal hypertension and confers the greatest risk. Despite advances made in the management of such cases, maternal morbidity remains high. Pregnancy is rare in women with liver cirrhosis due to reduced natural fertility rates. We report a case of two consecutive pregnancies in a woman with liver cirrhosis. A 29-year-old primipara with hepatitis B-related liver cirrhosis with portal hypertension who had been on tenofovir for the past seven years. The first pregnancy was spontaneously conceived but the second was by assisted conception. Both pregnancies were complicated by recurrent episodes of variceal bleeding that necessitated multiple endoscopic variceal band ligation (EVBL) during pregnancy. Medical advancement has enabled women with cirrhosis to have more pregnancies with reduced maternal and perinatal morbidities. Multidisciplinary management is vital to achieving favourable pregnancy outcomes.

Sanitary management and the presence of Salmonella spp. in a small-scale aviary and poultry carcasses

Salmonella spp. is one of the primary foodborne pathogens and is a problem for public health and poultry farming. This study aimed to evaluate the sanitary management of a small poultry farm of a family farming company under female management in Jataí-GO and how such management affects the presence of Salmonella spp. Samples were collected for microbiological analysis from the environment of two batches of chicks at the entrance (D0), on the 28th (D28) and the 60th (D60) day of the chicks; from the carcass, from the evisceration table and the chicken plucker. The presence of Salmonella spp. was not detected in any of the samples, which were taken from the bottoms of the transport boxes, from eggshell residues, from the growth and fattening rations, from shoe covers, from chicken litter, from the reception sheds, from the feeders and water fountains, from the walls and screens, from the external area of ​​the shed, from the compost pile and the carcasses. The pH, water chlorine, ambient temperature, and carcass temperature were assessed using a good production practices monitoring form and were considered adequate. The descriptive analysis of the data considered the current legislation. Therefore, strict monitoring of the standards underlying the Good Manufacturing Practices (GMP) and Standard Hygiene Operating Procedures (SHOP) programs controlled the spread of Salmonella spp. in the poultry meat rearing and processing process and also ensured the quality of the final product for consumption.

Glacial interglacial variations in the natural iron fertilization during the low sea ice periods along the eastern continental margin of Antarctica

The Southern Ocean sequesters atmospheric CO2 through biological pumps, though its driving factors are debated. Modern productivity is regulated by natural iron fertilization from micronutrient influx through dust, regeneration, and Antarctic glaciers and sea ice melting (ice melt). The productivity along the eastern Antarctic continental margin was low during the last glacial period and gradually increased through the deglacial to Late Holocene, marked by distinct productivity peaks. The micronutrients also varied similarly, with reduced glacial influx and an increase in the Holocene, which may cause productivity peaks. Therefore, the coherence of enhanced productivity and micronutrient influx is considered as enhanced iron fertilization period. The productivity peaks declined within ∼1.5 kyr, mostly due to the Ekman transport and sea ice formation. Along with ice melt, the independent weathering pattern that responds with glacial- interglacial ice volume changes suggest the source of micronutrients is not terrigenous. Shelf interaction of oceanic currents can influence the water column nutrient stock significantly, while its utilization occurs during reduced ice periods (low sea ice and high glacier melting) for productivity. Therefore, enhanced natural iron fertilization periods are considered as ice low periods that occurred in a periodicity of 2 kyrs, at ∼7.5 kyr BP, ∼5.5 kyr BP, ∼4 kyr BP, ∼ 2.5 kyr BP, and ∼0.5 kyr BP, likely due to the combined effects of atmospheric and oceanographic factors driven by the high amplitude regional temperature variability during the mid to late Holocene.

A process evaluation of a home garden intervention

BackgroundMost reviews of nutrition-sensitive programs assess the evidence base for nutrition outcomes without considering how programs were delivered. Process evaluations can fill this void by exploring how or why impacts were or were not achieved. This mid-term process evaluation examines a home garden intervention implemented in a large-scale, livelihoods improvement program in Odisha, India. The objectives are to understand whether the intervention was operating as planned (fidelity), investigate potential pathways to achieve greater impact, and provide insights to help design future home garden programs.MethodologyData collection and analysis for this theory-driven process evaluation are based on a program impact pathway that shows the flow of inputs, processes, outputs, outcomes, and impacts. Quantitative and qualitative data from focus group discussions, semi-structured interviews, and a Process Net-Mapping exercise with beneficiaries, frontline workers, and program management staff.ResultsDespite a mismatch between the design and implementation (low fidelity), the process evaluation identified positive outputs, outcomes, and impacts on home garden production, consumption, income, health and nutritional outcomes, and women’s empowerment. Flexibility led to greater positive outcomes on nutrition, the adoption of sustainable agricultural practices and easy-to-understand nutrition models, and the likelihood of the intervention being sustained after the program ends.ConclusionsTo help food systems in rural settings reduce food insecurity by utilizing more sustainable agricultural practices, we recommend that home garden interventions include instruction on easy-to-understand nutrition models and on how to make natural fertilizer. Finding local solutions like home gardens to help address critical supply issues and food insecurity is paramount.

Drivers of carbon stabilization and sequestration in Brazil’s black soils

Climate and land use are recognized as two of the main drivers of changes in soil organic carbon (SOC) on a global scale. Both factors play an important role in understanding SOC sequestration and mitigation of climate change. Particularly important, black soils are mineral soils with high SOC contents and high natural fertility and play an important role in national and global food and climate security. Here, we used a database of 90 black soils in Brazil − under different climate and land use conditions across the country − to test the hypothesis that C stock is richer in wetter climate conditions and that agricultural land use reduces C stock and the percentage of carbon saturation (PCS%). Climate data were obtained from the National Oceanic Atmospheric Administration (NOAA) and used to classify Thornthwaite’s climate. The land use information was obtained in the MapBiomas platform and was grouped into three major types: cropland, pasture, and native vegetation. The nonparametric Kruskal-Wallis test showed no differences for C stock, C/N ratio, and PCS% for both land use and climate. The low C/N ratio and the strong correlation between Ca2+, CEC, clay, and SOC suggest that organo-mineral interactions − which are stronger in soils with high-activity clays (e.g., Chernozems, Kastanozems, and Phaeozems) − promotes greater stabilization of the SOC and its long-term persistence and, thus being less sensitive to variations in climate and land use. Considering the total area of approximately 3.7 × 106 ha and the average value of C stock of 93.2 Mg/ha, the total SOC stored in Brazil’s black soils is in the order of 0.35 Gt, and the carbon stock stabilization potential is 0.25 Gt. Our results highlight the potential of Brazil’s black soils to promote carbon sequestration and climate change mitigation.

VALORIZATION OF FOOD WASTE THROUGH COMPOSTING PROCESSES

The food sector is one of the main sources of waste generation, and restaurants stand out for the abundance of food waste generated. It is estimated that 931 million tons of food waste (FW) will be generated in 2022, 26% of which will come from the food service sector. When improperly disposed of in the environment, FW causes problems such as nutrient leaching, pollutant gas emissions, and contamination, and is considered a public health problem. The objective of this study was to evaluate the effect of different stabilization bioprocesses on foodservice FW in terms of its physical and chemical characteristics, as well as the agronomic value of the final product. To this end, aerobic composting treatments were carried out for 100 days in closed compost bins. Three treatments were carried out, all in triplicate, with different proportions by volume of FW and grass clippings as a structuring agent. The performance of the process was evaluated in terms of temperature and mass and volume reductions, and the organic compounds were evaluated in terms of their physicochemical and spectroscopic characteristics. The composting results showed that the percentage of FW interfered with some parameters. Treatment T2, made with the highest percentage of FW, had the the lower values of pH, EC and EC:TOC ratio. The results led to the conclusion that the processes carried out are viable alternatives for recycling the nutrients contained in FSW.

The epidemiological declining in the human fertility rate in the arab world for 10 years period 2011–2021

Recently, there has been worldwide growing interest on profiling the human fertility of populations because there has been a noticeable global decline in fertility rate, leading to increased attention toward reproductive health and fertility.The decline in fertility of population of the Arab World was investigated for the 10 years period between 2011–2021. The Arab World was classified into three regional blocks; Block-1 Arabian Peninsula countries: Bahrain, Kuwait, Saudi Arabia, Oman, Qatar, United Arab Emirates (UAE), Yemen. Block-2 Fertile Crescent Arab countries: Iraq, Jordan, Lebanon, Syria, West Bank and Gaza. Block-3 African Arab countries: Algeria, Comoros, Djibouti, Egypt, Libya, Mauritania, Morocco, Somalia, Sudan, Tunisia. Data on fertility rates for the 10 years period between 2011–2021 were collected from the World Bank for Arab countries. Statistical analysis along with decline in the fertility rates were determined. Results: Fertility rates varied across Arab countries in 2011 and 2021, with notable decline ranging from 24.3% to 3.8%, except for Algeria, with zero decline. Countries that exhibited significant decline were Jordan (24.3%) followed by Iraq (22.2%) then Yemen (19.1%); Whereas, countries that exhibited slight fertility decline were Libya (3.8%), followed by Tunisia (4.5%), Lebanon (4.5%) and Kuwait (4.5%). On another note, lowest fertility rate was observed in UAE as maintained between 1.7% and 1.5% and the highest fertility rate was observed in Somalia as maintained between 7.3% and 6.3% for 2011 and 2021 respectively. Conclusion: The present study reveals the declining-trend in fertility rate across Arab countries, influenced by variable factors. Therefore, we recommend to the Council of the Health Ministries in the Arab-League to focus on investigating the fertility decline as an important parameter for public health in the Arab world to maintain natural balanced fertility rate.As some non-biological factors surrounding the Arabian region, such as instability, war, migration, the present study did not aim to include the influence of war and migration on fertility because both war and migration are non-biological external factors and both are not among the WHO criteria for fertility determination which based of the population growth rate of population under normal living conditions.

Biostabilization of fecal sludge and tannery liming sludge: A novel approach

Fecal sludge and tannery liming sludge management is essential for humans and the environment. The emitted amount of waste from two industries is reduced in composting leading to value-added products. This research focused on the effectiveness and feasibility of co-composting fecal sludge and hair-burning liming sludge from tannery. Fecal sludge was intermittently mixed with hair-burning liming sludge which also consisted of chicken manure and sawdust. Five piles (Pile 1, Pile 2, Pil3, Pile 4, and Pile 5) indicated respectively as P#1, P#2, P#3, P#4, and P#5, contained various ratios of composting materials were mixed, piled onto a horizontal bamboo frame, and observed for 120 days. To ensure a suitable oxygen supply, the composting piles were turned on in the thermophilic stage so that pathogens could not survive. The highest temperatures in the thermophilic stage were 39.0°C, 49.2°C, 55.7°C, 41.7°C, and 51.3°C. These referred to the respective piles P#1, P#2, P#3, P#4, and P#5. The Dewar stability index confirmed the stability of each composting pile, and the maximum degradation was found for P#3. The metals chromium (Cr), zinc (Zn), lead (Pb), iron (Fe), and nickel (Ni) in the final compost were found to be 38.1, 144.7, 15.1, 450.5, and 22.7 mg/kg, respectively. TCLP results reveal only an insignificant amount of metal leaching. Fecal coliform of the compost was below the standard level; Helminth eggs and Salmonella spp. were absent. SEM micrographs reflect the decomposition of composting materials. The maximum germination index and germination capacity of compost pile P#3 for compost-soil ratio 1:0 and 1:1 were 90%-92.8% and 100%, respectively. The present approach produced nutrient-enriched compost from fecal sludge and hair-burning liming sludge from a tannery emerges as a suitable solution for reducing solid wastes.

Male and female human papilloma virus infection and assisted reproductive technology outcomes: A comprehensive assessment from prevalence in semen to obstetric outcomes.

Infertility, affecting approximately 16% of the global population, has led to increased reliance on reproductive medicine. The impact of human papillomavirus (HPV) infection in one or both partners on outcomes of Assisted Reproduction Technologies (ART) remains unclear. This prospective cohort study aimed to evaluate prevalence and effects of HPV infection in subjects and couples candidates to ART. A total of n = 510 men and n = 246 women were included and n = 145 couples (n = 290 individuals) had both partners enrolled in the study. The HPV semen infection rate was 17% (95% CI: 14-20) with HPV-42, HPV-16, HPV-53 and HPV-51 as the most frequently detected genotypes. In women, 26% (95% CI: 21-32) tested HPV-positive in cervical swabs. In 6% (95% CI: 3-11) of the couples, both partners were positive but only three couples shared the same genotypes (HPV-16; HPV-39, HPV-51, and HPV-42; HPV-31). Follicular fluids were positive in 20% (95% CI: 11-33) of samples, showing genotype discrepancies with cervical tests. Semen treatment could not completely eliminate the virus in positive samples but reduced the positivity to one-third. No significant differences in semen and embryological variables, clinical pregnancy and live birth rates, neonatal and obstetrics outcomes were observed in subjects with positivity in semen or cervix compared to respective negative groups. Cumulative live birth rates per oocyte retrieval in couples where both partners were negative or both were positive did not differ, being 37% (95% CI: 28%-47%) and 44% (95% CI: 19-73), respectively. In conclusion, HPV testing should not be considered a prerequisite for accessing ART treatments. Robust inferences for natural fertility cannot be made using our findings, as the ART setting does not fully reflect natural conditions.

Ovarian reserve does not influence natural conception: insights from infertile women.

There is several albeit not univocal evidence suggesting that ovarian reserve is not related to the chance of natural pregnancy, provided that the remnant follicular pool is sufficient to ensure regular menstrual cycles. Nevertheless, available studies have some methodological limitations, and the issue cannot be considered definitively ascertained. To further address this issue, we retrospectively selected infertile women whose infertility diagnostic work-up was unremarkable (unexplained infertility-cases) and matched them by age and study period to a group of infertile women who were diagnosed with severe male infertility (controls). If ovarian reserve impacts on natural fertility, one had to expect lower ovarian reserve among women with unexplained infertility. Tested biomarkers included AMH, AFC and day 2-3 serum FSH. The primary aim was the frequency of women with serum AMH < 0.7ng/ml. Two-hundred fifty-two women with unexplained infertility and 252 women with male infertility were included. All biomarkers of ovarian reserve did not differ between the study groups. AMH levels < 0.7ng/mL were observed in 26 (10%) women with unexplained infertility and 35 (14%) women with male infertility (p = 0.28). The adjusted OR was 0.76 (95% CI: 0.44-1.33). Significant differences did not also emerge when repeating this dichotomous analysis using other biomarkers and other thresholds for the definition of low-ovarian reserve. This study confirms that ovarian reserve is unremarkable to natural conception. Physicians and patients should be aware of this concept to avoid inappropriate counseling and undue clinical decisions.

New insights into the ovulatory process in the human ovary.

Successful ovulation is essential for natural conception and fertility. Defects in the ovulatory process are associated with various conditions of infertility or subfertility in women. However, our understanding of the intra-ovarian biochemical mechanisms underlying this process in women has lagged compared to our understanding of animal models. This has been largely due to the limited availability of human ovarian samples that can be used to examine changes across the ovulatory period and delineate the underlying cellular/molecular mechanisms in women. Despite this challenge, steady progress has been made to improve our knowledge of the ovulatory process in women by: (i) collecting granulosa cells across the IVF interval, (ii) creating a novel approach to collecting follicular cells and tissues across the periovulatory period from normally cycling women, and (iii) developing unique in vitro models to examine the LH surge or hCG administration-induced ovulatory changes in gene expression, the regulatory mechanisms underlying the ovulatory changes, and the specific functions of the ovulatory factors. The objective of this review is to summarize findings generated using in vivo and in vitro models of human ovulation, with the goal of providing new insights into the mechanisms underlying the ovulatory process in women. This review is based on the authors' own studies and a search of the relevant literature on human ovulation to date using PubMed search terms such as 'human ovulation EGF-signaling', 'human ovulation steroidogenesis', 'human ovulation transcription factor', 'human ovulation prostaglandin', 'human ovulation proteinase', 'human ovulation angiogenesis' 'human ovulation chemokine', 'human ovulatory disorder', 'human granulosa cell culture'. Our approach includes comparing the data from the authors' studies with the existing microarray or RNA-seq datasets generated using ovarian cells obtained throughout the ovulatory period from humans, monkeys, and mice. Current findings from studies using in vivo and in vitro models demonstrate that the LH surge or hCG administration increases the expression of ovulatory mediators, including EGF-like factors, steroids, transcription factors, prostaglandins, proteolytic systems, and other autocrine and paracrine factors, similar to those observed in other animal models such as rodents, ruminants, and monkeys. However, the specific ovulatory factors induced, their expression pattern, and their regulatory mechanisms vary among different species. These species-specific differences stress the necessity of utilizing human samples to delineate the mechanisms underlying the ovulatory process in women. The data from human ovulation in vivo and in vitro models have begun to fill the gaps in our understanding of the ovulatory process in women. Further efforts are needed to discover novel ovulatory factors. One approach to address these gaps is to improve existing in vitro models to more closely mimic in vivo ovulatory conditions in humans. This is critically important as the knowledge obtained from these human studies can be translated directly to aid in the diagnosis of ovulation-associated pathological conditions, for the development of more effective treatment to help women with anovulatory infertility or, conversely, to better manage ovulation for contraceptive purposes. N/A.

Isolation, characterization, and genetic manipulation of cold-tolerant, manganese-oxidizing Pseudomonas sp. strains.

Manganese-oxidizing bacteria (MnOB) produce Mn oxide minerals that can be used by humans for bioremediation, but the purpose for the bacterium is less clear. This study describes the isolation and characterization of cold-tolerant MnOB strains isolated from a compost pile in Morris, Minnesota, USA: Pseudomonas sp. MS-1 and DSV-1. The strains were preliminarily identified as members of species Pseudomonas psychrophila by 16S rRNA analysis and a multi-locus phylogenetic study using a database of 88 genomes from the Pseudomonas genus. However, the average nucleotide identity between these strains and the P. psychrophila sp. CF149 type strain was less than 93%. Thus, the two strains are members of a novel species that diverged from P. psychrophila. DSV-1 and MS-1 are cold tolerant; both grow at 4°C but faster at 24°C. Unlike the mesophilic MnOB P. putida GB-1, both strains are capable of robustly oxidizing Mn at low temperatures. Both DSV-1 and MS-1 genomes contain homologs of several Mn oxidation genes found in P. putida GB-1 (mnxG, mcoA, mnxS1, mnxS2, and mnxR). Random mutagenesis by transposon insertion was successfully performed in both strains and identified genes involved in Mn oxidation that were similar to those found in P. putida GB-1. Our results show that MnOB can be isolated from compost, supporting a role for Mn oxidation in plant waste degradation. The novel isolates Pseudomonas spp. DSV-1 and MS-1 both can oxidize Mn at low temperature and likely employ similar mechanisms and regulation as P. putida GB-1.IMPORTANCEBiogenic Mn oxides have high sorptive capacity and are strong oxidants. These two characteristics make these oxides and the microbes that make them attractive tools for the bioremediation of wastewater and contaminated environments. Identifying MnOB that can be used for bioremediation is an active area of research. As cold-tolerant MnOB, Pseudomonas sp. DSV-1 and MS-1 have the potential to expand the environmental conditions in which biogenic Mn oxide bioremediation can be performed. The similarity of these organisms to the well-characterized MnOB P. putida GB-1 and the ability to manipulate their genomes raise the possibility of modifying them to improve their bioremediation ability.

Biochar promotes compost humification by regulating bacterial and fungal communities.

Humus can be formed during composting through biological pathways, nonetheless, the mechanisms through which bacterial and fungal communities govern the development of humus in compost with the addition of biochar remain uncertain. In this study, compost with cow dung and maize stover as feedstock was employed as a control group, and compost with 10% biochar added on top of the feedstock was adopted as a treatment group to investigate the effect of bacterial and fungal communities on humus formation during biochar composting. The results demonstrated that the humic acid content increased by 24.82 and 25.10% at the cooling and maturation stages, respectively, after adding biochar. Besides, the degree of polymerization content in the maturation stage was elevated by 90.98%, which accelerated the humification process of the compost. During the thermophilic and maturity stages, there was a respective increase of 51.34 and 31.40% in reducing sugar content, suggesting that the inclusion of biochar could furnish ample reducing sugar substrate for the Maillard reaction. The addition of biochar reduced the number of humus precursor-associated genera by 35, increased the number of genera involved in humus synthesis by two, and enhanced the stability of the cross-domain network between bacteria and fungi, which confirms that microorganisms contribute to the humification process by decreasing humus precursor consumption as well as increasing humus synthesis with the addition of biochar. Additionally, adding biochar could enhance the humification capacity of the compost pile by dominating the Maillard reaction with reducing sugars as the substrate and strengthening the function of humus synthesis-associated genera. This study enhances our comprehension of the regulatory pathways of biochar in the humification process during composting.

New Records of Soil Galumnid Mites (Acarina: Cryptostigmata) from Dampa Tiger Reserve, Mizoram, India

Among all the soil micro- arthropods, mites are predominant and playing important role in increasing soil fertility through organic decomposition and they are inhabiting in all types of soil but predominantly found in soil litter, humus, and compost heaps. The present research work has been done to identify and prepare a record of the soil Galumnid mites from this region as very few works has been done on the taxonomy of this important group in this geographical area as well as in India. Only one major work has been found which was published by Sanyal (2009)[1] on the study of oribatid mites of Mizoram. Amongst all the States of India, Mizoram has the highest area under forest cover in terms of percentage of geographical area. Dampa Tiger Reserve of western Mizoram of India is located at Indo-Myanmar hotspot in the Lushai hills is one of the remotes Tiger Reserve of the country and immensely rich in floral and faunal biodiversity. 9 species including 5 genera of Galumnid mites are newly recorded for the first time from this area of study.