Soil organic carbon pools have an important role in the maintenance of ecosystems as a source of energy for soil microorganisms. Soil biological and biochemical properties are essential for the decomposition of organic matter. These soil properties can be affected by thinning, which is considered sustainable when the soil properties are maintained or improved. We studied the effects of selective thinning and shrub clearing, performed with an ecohydrological approach, in a marginal Holm oak forest in a semiarid area on soil properties. The effects of thinning (T) were compared with an untreated area (control, C). Fine woody debris was ground into mulch onto the thinned area. Forest floor and mineral soil properties were analyzed between five months and seven years after the thinning. In the forest floor, gravimetric water content (GWCff) and water soluble organic carbon (WSOCff) were analyzed and compared between T and C. In mineral soil, GWCms, soil organic carbon (SOC), WSOCms, soil basal respiration (BR), soil microbial biomass carbon (MBC) and soil enzymes (acid phosphatase (Acid PA) and urease (URE)) were analyzed. In the early stage, the results showed slightly higher SOC and WSOCms in T likely due to fine woody debris left on the forest floor. However, seven years after the thinning the effects of the thinning on all the studied variables were negligible. All variables showed high spatial-temporal variability. Our results suggest that selective thinning and shrub clearing in the studied site do not affect negatively soil properties when woody debris is left on the forest floor.