Hippocamelus Bisulcus Research Articles

Mycobacterium avium subsp. paratuberculosis (MAP) infection in the endangered huemul deer (Hippocamelus bisulcus) in Patagonia

In a huemul (Hippocamelus bisulcus) population sympatric with cattle, we found evidence of Mycobacterium avium subsp. paratuberculosis (MAP) infection. Three huemul faecal pellet samples and two cows pats were collected and cultured for MAP presence. DNA was then extracted for PCR analysis of all signal-positive cultures. To assess whether MAP isolates obtained from huemul faeces were associated with typical MAP isolated from livestock, positive confirmed culture samples were sub-typed using a combination of five Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat Analysis and one Short Sequence Repeat analysis markers. All faecal samples from both species were MAP positive. One huemul presented a different bacteria profile genotype not described before, suggesting that huemul and cattle in Patagonia could carry a unique MAP strain.

The next frontier for recovering endangered huemul (Hippocamelus bisulcus): how to avoid recurrent misdiagnoses of health status and risks

Abstract Context The currently remaining 350–500 huemuls in Argentina are not recovering. We evaluated live huemuls, along with animals that died soon after confinement, or those that had died recently. Although information on the health status is highly valuable, repeated misdiagnoses of the health status indicate a need for other strategies. Aims . Discrepancies between clinical and postmortem diagnoses are critical for improving subsequent management decisions. Methods Initial clinical interpretations and risk assessments were reinterpreted on the basis of necropsies and other data. Results Two debilitated huemul individuals examined by veterinarians died soon afterwards, supposedly one being intoxicated and one being without lesions. Necropsies showed osteopathology and fluorosis (fluorine concentrations of 2209 and 2979 mg/kg). Another male was tied up, with authorities and veterinarians arriving the next day. After being sedated, and judged healthy, the animal was translocated. Because there was no reversal, this animal died 22 h post-capture. Exhumation showed severe osteopathology. Elsewhere, huemuls were considered adequate in selenium because values below the detection limit were excluded. However, when all values were included, 75% of the animals were selenium-deficient; this population had numerous cases of osteopathology. Recently, specialists went to Torres del Paine Park suspecting caseous lymphadenitis, reporting of which has been obligatory since 1937. However, many cases documented in 1999–2007 have not elicited responses since that time by health professionals. Selenium deficiency negatively affects antibody responses against caseous lymphadenitis. One province had denied huemul capture (2012 and 2013) on recommendation of scientific advisors. Because of the right for transparency, it was found out in 2016 that authorities had requested advice from only one veterinarian who assessed that darting was too risky. Another 2016 project proposed to dart the first huemul in Argentina. Two weeks earlier, that same team was called to rescue a tied-up huemul; the team opted not to involve a laboratory with drugs and radios that was only 1 h away. This huemul died and was left in the woods. Finally, the first huemul enclosure in Argentina was proposed (1995), but the permission was denied. Again, in 2000, the first huemul centre with private funding secured for 30 years was proposed. However, the Regional Delegation for Patagonian National Parks prevented aerial surveys, and advised not to provide a permit for the centre. Conclusions Future assessments should consider osteopathology. Risk assessments should be transparent and based on assessment by multiple qualified professionals. Implications Clinical misdiagnoses may reduce life expectancy, in contrast to taking individuals to enclosures, which would also allow valuable reintroductions. Not permitting captures, censusses and enclosures has resulted in unwarranted delays in conservation progress.

Diagnóstico de parásitos en heces del huemul (Hippocamelus bisulcus) en el centro y sur de Chile

Objetivo. Identificar parásitos en heces del huemul (Hippocamelus bisulcus) presente en áreas naturales protegidas del centro y sur de Chile y evaluar diferencias en la prevalencia de parásitos entre regiones y estaciones del año. Materiales y métodos. 200 muestras de heces de huemul fueron colectadas de distintas localidades de las regiones de Ñuble, Aysén y Magallanes a lo largo de todas las estaciones entre los años 2006 y 2008. Las muestras fueron analizadas utilizando las técnicas de flotación y sedimentación, para ser posteriormente observadas en el microscopio con el objetivo de detectar parásitos. Adicionalmente, se analizaron 124 de estas muestras para determinar la presencia de Fasciola hepatica. Resultados. El 38.0%(n=76/200) de las muestras analizadas fueron positivas a algún parásito, observándose una prevalencia de 11.8%(n=2/17) en Ñuble, 22.8%(n=28/123) en Aysén y un 76.7%(n=46/60) en Magallanes. En Ñuble se encontraron huevos de Moniezia sp., mientras que en Aysén y Magallanes se identificaron huevos tipo Estrongílido, Nematodirus sp. y ooquistes de Eimeria spp. No se evidenció F. hepatica en las muestras analizadas. Se detectan diferencias de parasitismo entre las estaciones (p≤0.05) en Aysén y Magallanes. Conclusiones. La diferencia en la prevalencia y diversidad parasitaria entre regiones incluidas en este estudio pueden estar relacionadas con la disimilitud en la densidad poblacional de huemules y la masa ganadera de cada región. Es necesario continuar con monitoreos parasitológicos en poblaciones de huemul en Chile sur y central y estudiar la relación de esta especie con el ganado y la posible transmisión inter- e intraespecífica de parásitos.

Putative parapoxvirus-associated foot disease in the endangered huemul deer (Hippocamelus bisulcus) in Bernardo O'Higgins National Park, Chile.

The huemul (Hippocamelus bisulcus) is an endangered cervid endemic to southern Argentina and Chile. Here we report foot lesions in 24 huemul from Bernardo O’Higgins National Park, Chile, between 2005 and 2010. Affected deer displayed variably severe clinical signs, including lameness and soft tissue swelling of the limbs proximal to the hoof or in the interdigital space, ulceration of the swollen tissues, and some developed severe proliferative tissue changes that caused various types of abnormal wear, entrapment, and/or displacement of the hooves and/or dewclaws. Animals showed signs of intense pain and reduced mobility followed by loss of body condition and recumbency, which often preceded death. The disease affected both genders and all age categories. Morbidity and mortality reached 80% and 40%, respectively. Diagnostics were restricted to a limited number of cases from which samples were available. Histology revealed severe papillomatous epidermal hyperplasia and superficial dermatitis. Electron microscopy identified viral particles consistent with viruses in the Chordopoxvirinae subfamily. The presence of parapoxvirus DNA was confirmed by a pan-poxvirus PCR assay, showing high identity (98%) with bovine papular stomatitis virus and pseudocowpoxvirus. This is the first report of foot disease in huemul deer in Chile, putatively attributed to poxvirus. Given the high morbidity and mortality observed, this virus might pose a considerable conservation threat to huemul deer in Chilean Patagonia. Moreover, this report highlights a need for improved monitoring of huemul populations and synergistic, rapid response efforts to adequately address disease events that threaten the species.

Echinococcus Granulosus in the Endangered Patagonian Huemul (Hippocamelus bisulcus)

Cystic echinococcosis (CE) is a zoonotic parasitic disease associated with Echinococcus granulosus. The parasite is maintained by domestic and wild canids as definitive hosts with several ungulate species as intermediate hosts in domestic and peridomestic transmission cycles. In Chile, CE is endemic, and the role of livestock and dogs (Canis lupus familiaris) in the cycle and the accidental infection of humans are widely documented at rural sites. However, the role of wild herbivores in wild cycles or the potential transmission of CE from livestock is still unknown in Chile and the rest of South America. We used molecular techniques to describe CE infecting a Patagonian huemul (Hippocamelus bisulcus) in Cerro Castillo National Reserve (Aysén region, Chile). We make inferences about the risk of disease spillover from sympatric domestic and wild species. The DNA-based molecular analysis revealed that the huemul was infected with E. granulosus G1 genotype, sharing haplotypes with other G1 samples collected from sheep (Ovis aries) and cattle (Bos taurus) worldwide. Geographic overlap between sheep and huemul populations in the reserve likely facilitates parasite spillover into wild deer populations, with shepherd or stray dogs and wild foxes (Lycalopex culpaeus) potentially acting as bridging hosts between livestock and the endangered huemul. Further studies are warranted to understand the implications of E. granulosus for huemul conservation throughout the Chilean Patagonia.

Protected areas' effectiveness under climate change: a latitudinal distribution projection of an endangered mountain ungulate along the Andes Range.

BackgroundClimate change is one of the greatest threats to biodiversity, pushing species to shift their distribution ranges and making existing protected areas inadequate. Estimating species distribution and potential modifications under climate change are then necessary for adjusting conservation and management plans; this is especially true for endangered species. An example of this issue is the huemul (Hippocamelus bisulcus), an endemic endangered deer from the southern Andes Range, with less than 2,000 individuals. It is distributed in fragmented populations along a 2,000 km latitudinal gradient, in Chile and Argentina. Several threats have reduced its distribution to <50% of its former range.MethodsTo estimate its potential distribution and protected areas effectiveness, we constructed a species distribution model using 2,813 huemul presence points throughout its whole distribution range, together with 19 bioclimatic layers and altitude information from Worldclim. Its current distribution was projected for years 2050 and 2070 using five different Global Climate Models estimated for scenarios representing two carbon Representative Concentration Routes (RCP)—RCP4.5 and RCP6.0.ResultsBased on current huemul habitat variables, we estimated 91,617 km2 of suitable habitat. In future scenarios of climate change, there was a loss of suitable habitat due to altitudinal and latitudinal variation. Future projections showed a decrease of 59.86–60.26% for the year 2050 and 58.57–64.34% for the year 2070 according to RCP4.5 and RCP6.0, respectively. Protected areas only covered only 36.18% of the present distribution, 38.57–34.94% for the year 2050 and 30.79–31.94% for 2070 under climate change scenarios.DiscussionModeling current and future huemul distributions should allow the establishment of priority conservation areas in which to focus efforts and funds, especially areas without official protection. In this way, we can improve management in areas heavily affected by climate change to help ensure the persistence of this deer and other species under similar circumstances worldwide.

Modeling of potential habitat suitability of Hippocamelus bisulcus: effectiveness of a protected areas network in Southern Patagonia

IntroductionHuemul (Hippocamelus bisulcus Molina) is the most threatened flag species of Southern Patagonia, where conservation efforts were not effective to avoid the retraction of its distribution area. Habitat quality modeling can assist to design better management strategies for regional conservation planning. The objective was to elaborate one habitat suitability map for huemul, defining the environmental characteristics at landscape level, and determining the distribution of the suitable habitat inside the current natural reserve network.MethodsWe used a database of 453 records and explored 40 potential explanatory variables (climate, topographic, and landscape variables including human-related ones) to develop one habitat suitability map using the Environmental Niche Factor Analysis (ENFA) for Santa Cruz province (Argentina). We combined the outputs in a GIS project using different shapes, including the current natural reserve network.ResultsWe defined the potential habitat for huemul, where forest edges and ecotone zones (e.g., mainly alpine environments) were the most important environmental variables, as well as some forest types (e.g., Nothofagus pumilio). Habitat losses were found in the extreme potential distribution areas (northern and southern areas), probably related to the increasing ranch activities. The current natural reserve network maintains approximately half of the huemul potential habitat in Santa Cruz province, where National Parks presented the similar conservation importance than the Provincial natural reserves.ConclusionsHabitat suitability model for huemul can be used as a decision support system for new management strategies at different landscape levels to improve the current conservation efforts.

Ancient parasites from endemic deer from "CUEVA PARQUE DIANA" archeological site, Patagonia, Argentina.

The narrow Andean-Patagonian temperate rainforest strip in the west of southern South America is inhabited by two endemic species of cervids, the southern pudu (Pudu puda) and the huemul (Hippocamelus bisulcus), both cataloged as near threatened and threatened species, respectively. One of the possible causes of their declined number is the susceptibility to livestock diseases. Significant zooarchaeological records of both deer have been found throughout the Holocene from Patagonia. The present contribution reports the first paleoparasitological results obtained from coprolites of endemic deer from the archeological site "Cueva Parque Diana," Neuquén Province, Argentina, and discusses the possible diseases found in ancient times. Thirty-four coprolites were fully processed, rehydrated, homogenized, sieved, subjected to spontaneous sedimentation, and examined by light microscopy. Thirty samples contained parasite remains. The presence of diverse parasitic diseases such as trematodioses, metastrongylosis, trichuriosis, strongylida gastroenteritis, dioctophymosis, and coccidiosis which could cause diseases in deer previous to the arrival of European livestock and the presence of zoonotic diseases in the hunters-gatherers and fishermen are discussed.

Corynebacterium pseudotuberculosis Infection in Patagonian Huemul (Hippocamelus bisulcus)

Corynebacterium pseudotuberculosis is an intracellular bacteria and the etiologic agent of caseous lymphadenitis in domestic and wildlife species. We report C. pseudotuberculosis infection in Patagonian huemul ( Hippocamelus bisulcus ) from the Cerro Castillo National Reserve, Region of Aysen, Chile. Subcutaneous abscesses in the abdominal and pectoral regions from two animals were sampled and bacteriologic isolation was performed. In both cases, we isolated a C. pseudotuberculosis strain belonging to the ovine genotype. In addition, one isolate was resistant to ciprofloxacin and streptomycin. We report that H. bisulcus is a susceptible species to this bacterium, which is transmitted by direct or indirect contact with domestic sheep ( Ovis aries ) and which represents a potential conservation threat to populations of H. bisulcus . Additional research and prevention efforts should be addressed.

Distributional patterns of living ungulates (Mammalia: Cetartiodactyla and Perissodactyla) of the Neotropical region, the South American transition zone and Andean region

To recognize the distributional patterns of living ungulates in the Neotropical region, the South American transition zone, and Andean region using the panbiogeographical method of track analysis, and to attempt to correlate these patterns with geological history. The distribution of 24 species of living ungulates (in the families Camelidae, Cervidae, Tapiridae and Tayassuidae) was studied by the panbiogeographical method of track analysis. It was performed using distributional data acquired from literature and databases of scientific institutions. Individual tracks were obtained for each species by plotting locality records on maps and connecting them by minimum-spanning trees. Generalized tracks were determined from the spatial overlap between individual tracks, indicating a common history. The intersection between generalized tracks defined a biogeographic node, implying that these locations are biogeographic composites resulting from different ancestral biotas coming into spatial contact, possibly at different geologic times. The superposition of the 24 individual tracks resulted in five generalized tracks (GTs): GT1, Mesoamerican/Choco (composed of Mazama pandora, Mazama temama, Odoicoileus virginianus and Tapirus bairdii); GT2, Northern Andes (Mazama rufina, Pudu mephistophiles and Tapirus pinchaque); GT3, Central Andes (Hippocamelus antisensis, Lama guanicoe, Mazama chunyi and Vicugna vicugna); GT4, Chilean Patagonia (Hippocamelus bisulcus and Pudu puda); and GT5, Chaco/Central west Brazil (Blastocerus dichotomus, Catagonus wagneri and Ozotocerus bezoarticus). The biogeographic node was found in the Northwestern Colombia. The geological events such as tectonism and volcanism that occurred through the Neogene and mainly in the Pleistocene caused fragmentation, diversification and endemism of biota. The biogeographic node in Colombia occurred within a zone of convergence. This node emphasized the complexity of the area and it contains biotic elements with different origins, which represent a special condition for the establishment of priority conservation areas.

Predicting the potential distribution of the Endangered huemul deer Hippocamelus bisulcus in North Patagonia

AbstractHabitat loss is one of the main threats to wildlife, particularly large mammals. Estimating the potential distribution of threatened species to guide surveys and conservation is crucial, primarily because such species tend to exist in small fragmented populations. The Endangered huemul deer Hippocamelus bisulcus is endemic to the southern Andes of Chile and Argentina. Although the species occurs in the Valdivian Ecoregion, a hotspot for biodiversity, we have no information on its occupancy and potential distribution in this region. We built and compared species distribution models for huemul using the maximum entropy approach, using 258 presence records and sets of bioclimatic and geographical variables as predictors, with the objective of assessing the potential distribution of the species in the Valdivian Ecoregion. Annual temperature range and summer precipitation were the predictive variables with the greatest influence in the best-fitting model. Approximately 12,360 km2 of the study area was identified as suitable habitat for the huemul, of which 30% is included in the national protected area systems of Chile and Argentina. The map of potential distribution produced by our model will facilitate prioritization of future survey efforts in other remote and unexplored areas in which huemul have not been recorded since the 1980s but where there is a high probability of their occurrence.

Assessing productive lands as viable habitat for huemul in patagonia

The creation of protected areas has been the main strategy to counter loss and fragmentation of habitats for large mammals, but these areas by themselves cannot guarantee species’ conservation. Forestry plantations can provide habitat for a large range of species. However, studies assessing the impact of forestry plantations on large mammals are scarce. Our objective was to identify the environmental variables that explained the presence of the endangered huemul deer (Hippocamelus bisulcus) in an area with mixed pine plantation and native forest in the Chilean Patagonia. Occupancy models using data collected from camera traps indicated higher occupancy in plantation areas (0.70) than in native forest (0.50). The main variable explaining the presence of the huemul in both forest types was the understory cover between 21 cm and 50 cm in height. By maintaining understory cover, plantations provide a suitable complementary habitat for the forest-dwelling huemul. © 2016 The Wildlife Society.

El uso de la fauna en Laguna Cóndor, Provincia de Santa Cruz, Argentina

a ABSTRACT This paper presents the results of the analysis of faunal assemblages recovered at the archaeological locality of Laguna Condor, on the upper basin of the Gallegos River at the Morros area, Santa Cruz, Argentina. Despite the low rate of bone deposition, high densities of highly fragmented bones were recorded. A high diversity of species was identified, although only one taxon was intensively exploited. The focus was on the exploitation of Lama guanicoe and, occasionally, forest resources (Hippocamelus bisulcus), and European fauna (Equus caballus). The evidence reinforces the interpretation of Laguna Condor as logistically, redundantly and intensively exploited place during short periods ca. 200 years AP.

Osteopathology and selenium deficiency co-occurring in a population of endangered Patagonian huemul (Hippocamelus bisulcus).

BackgroundAbout 1,000 endangered Patagonian huemul deer (Hippocamelus bisulcus) remain in Chile and 350–500 in Argentina. Most groups (>100) are not recovering, and prevalence of osteopathology in Argentina was at least 57%. Here I describe relevant cases of osteopathology from a Chilean population which, however, recently also provided data on trace mineral status, supporting the initial hypothesis that nutrition may be a primary etiologic factor. Additionally, recent data on bone chemical composition of Argentine cases and soil analyses are discussed.ResultsFluoride levels in Argentine cases with osteopathology were low and fluorosis was discarded as an etiological factor. Selenium deficiency occurred in 73% of huemul from the Chilean population which exhibited several cases with osteopathology. The pathophysiognomy included extensive erosion; tooth loss; porosification; perforations of palate, maxillar and mandibular bone with frequent exposure of tooth roots; and fractured mandibula. Areas currently used by remaining huemul have mainly acidic volcanic soils, which reduces selenium bioavailability: mean soil selenium levels from areas typically used by extant huemul were very deficient (0.19 ppm), corroborating documented overt selenium deficiency in local livestock and plants. The area of extant huemul is known to result in primary iodine deficiency in livestock which is aggravated by selenium deficiency.ConclusionsCurrently the most parsimonious explanation for frequent osteopathology and lack of numerical recovery are the combined effects of selenium and iodine deficiencies based on: osteopathology in a population of selenium deficient huemul; selenium deficient livestock, plants and soils; acidic soils; and regional primary iodine deficiency. The nexus between mineral nutrition and population dynamics of huemul may be due to constraints on their movements to fertile lowlands, including the elimination of historic migratory traditions, and concomitant elimination of source populations.

Relationships Between Value Orientations and Wildlife Conservation Policy Preferences in Chilean Patagonia

Conflicts over wildlife conservation in protected areas can occur because stakeholders hold divergent values and value orientations. In this exploratory study, differences in value orientations among visitors to Chile’s Tamango National Reserve (TNR) were examined. Questionnaires were completed by visitors (n = 97) during the Chilean summer of 2012. Respondents were grouped into strong protection (63%) and mixed protection–use (37%) value orientation groups using cluster analysis. Mixed protection–use group members were more likely to be local residents, less formally educated, less likely to pay the reserve entry fee, and less supportive of huemul (Hippocamelus bisulcus) conservation policies compared to the strong protection group. Most TNR visitors would support policies that protect wildlife in the reserve, and development with deleterious effects on the huemul would be viewed less favorably. It is suggested that a post-material shift and place relations moderate relationships between value orientations and Chilean wildlife conservation policy preferences.

Incorporating preferential prey selection and stochastic predation into population viability analysis for rare prey species

There is increasing evidence that predation can cause the decline and extinction of small populations of prey, and that stochastic predation resulting from variation in prey selection by individual predators can have significant consequences for population persistence. Modelling approaches that ignore variation in prey selection exhibited by individual predators may inaccurately predict the effect of predation on prey populations, especially over longer time scales. We assess the impacts of variation in prey selection by building PVA models for endangered huemul deer Hippocamelus bisulcus that sequentially include and exclude observed stochasticity in predation among individual pumas Puma concolor. Our results indicated that huemul are at risk of extinction in all scenarios modelled, although the immediacy of this risk differed based on model structure and time period considered. Specifically, modelling predation as a random effect based on an interrupted Poisson process rather than as a directional and continuous change in survival rates, resulted in significantly longer estimates of time to extinction independent of the assumed intensity of predation. Our results highlight the importance of determining whether specialist predators are driving predation on rare prey, and when they are, incorporating said stochastic predation when attempting to predict persistence probabilities of rare prey using PVA models. Since results of PVA models are commonly used to develop conservation strategies, we advocate for the inclusion of stochastic predation in future PVA models where warranted to more accurately inform strategies for the conservation of rare prey and their predators.

Morphometric analysis of modern faeces as a tool to identify artiodactyls' coprolites

The identification of the zoological origin of coprolites is important to infer paleoecological characteristics of ancient wildlife. The aim of this study was to design a reliable tool to distinguish coprolites of artiodactyls collected from archaeological sites of Patagonia, Argentina. A reference collection of modern droppings of large size artiodactyls of Patagonia, Lama guanicoe (guanaco) and Hippocamelus bisulcus (huemul), was also studied. Generalized discriminant analysis using shape and size attributes was applied to 538 pellets (nguanaco = 240, nhuemul = 298). Faecal material was photographed and multiple predictor variables were calculated by the Image J (Java 1.6.0_12) Program. Coprolites attributable to camelids by paleoparasitological analyses were also classified by the discriminant classification function obtained from the reference collection. Results indicated that pellet size and shape were able to discriminate droppings of guanaco from those of huemul (Pillai's trace = 0.93, p = 0.0001). The accuracy of the classification function was 100%, with all the modern faeces of guanaco and huemul correctly classified. The accuracy for the identification of camelid coprolites was 100%. This is the first study that used a discriminant classification function of modern dung to identify coprolites. This research also provides information for its potential application to other archaeological deposits of dung in order to confirm the zoological origin of samples.

Cross-amplification of nonspecific microsatellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer.

Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully in noninvasive samples from these species. We also compared these nonspecific loci against eight polymorphic loci that were recently developed for huemul to determine whether the nonspecific markers could reflect the huemul's genetic variation that was observed with the specific loci. We identified 10 suitable loci, six of which constitute a standardized set for the two species and can be used to identify them in the absence of phenotypic data. The expected heterozygosity per locus for the panel of six loci ranged from 0.461 to 0.889 (average 0.665), and the maximum probability of identity value was 6.9x10(-6) and 3.2x10(-4) in pudu and huemul, respectively. This set of loci has potential applications in evolutionary, ecological, forensic, and conservation studies in pudu and huemul.

Blood Mineral Concentrations in the Endangered Huemul Deer (Hippocamelus bisulcus) from Chilean Patagonia

Concentrations of calcium, magnesium, copper, zinc, and selenium were measured in plasma from 11 huemul (Hippocamelus bisulcus) from Chilean Patagonia. Except for zinc and copper, concentrations of these minerals were similar to those of other deer species.

δ13C and δ15N Characterization of Modern Huemul (Hippocamelus bisulcus) from the Patagonian Andean Forest. Scope and Limitations of Their Use as a Geographical Marker

ABSTRACTThis paper presents an isotopic characterization (δ13C and δ15N) of modern huemul from Patagonian Andean forest. This deer is considered an endemic species, now inhabiting the sub‐Antarctic forest of Chile and Argentina. We analyse if the isotopic signals of the modern huemuls can be used as geographic markers on two distinct spatial scales. Firstly, on the intra‐forest level, we analyse the relation between the δ13C and δ15N of the huemul and the annual precipitation in the place in which the sample was collected. Secondly, on the inter‐environmental level, we evaluate to what extent the huemul's isotopic signal differs from that of the guanaco (Lama guanicoe), an herbivore that occupies Patagonia's continental steppe. The results reveal that, on the one hand, there is no relation between the isotopic values of modern huemules and precipitation levels, whereas on the other hand, the modern huemul is different from the guanaco in both isotopes. We believe that this difference between the herbivores is associated with the isotopic signals at the base trophic chain, which is influenced by the precipitation gradient. In this way, even though for now, these isotopic markers turn out not to be useful for differentiating between huemuls coming from sectors of the forest with marked differences in precipitation, it is possible to distinguish herbivores coming from the forest and steppe of continental Patagonia, enabling the use of δ13C and δ15N as geographic markers. The isotopic characterization of δ13C and δ15N of the modern huemuls and its relation to the Patagonian Andean forest has strong implications for paleoecological and archaeological aspects of this environment, including the biogeographical history of the huemul, and in order to the test the models of use and exploitation of the forest by hunter‐gatherers in the past. Copyright © 2013 John Wiley &amp; Sons, Ltd.