The pollutant concentration and hygiene standards in mountain tunnels are key factors determining the ventilation effectiveness of tunnel operation. Previous studies have mainly focused on the spatiotemporal distribution patterns of in-tunnel pollutants, while research on ventilation control indexes for pollutants in long mountain tunnels are relatively limited. Therefore, this paper aims to discussing the hygiene control indexes for operational ventilation of mountain tunnels. Field tests were conducted in the No.1 Qinling Tunnel of Xi'an to Han zhong Highway in China, and the distribution laws of traffic flow, wind speed, and concentrations of particulate matter (PM), carbon monoxide (CO), and nitrogen oxide (NOX) were on-site measured. The spatiotemporal distribution characteristics of pollutants in the long mountain tunnel were revealed, and the ventilation hygiene control indexes for harmful gases were calculated and compared to those of urban highway tunnels. The results show that the traffic volume of operational highway tunnels in mountainous areas of China is increasing rapidly, and the particulate matter emitted from diesel vehicle exhaust will pose a threat to the normal environmental quality in the tunnel. The average value of CO base emission factor in 2000 for the upline of No.1 Qinling tunnel was 0.0019 m3/(veh·km) by back-calculation, and the annual decline rate of CO in the ventilation design for mountain highway tunnels should be 6 %–8 % and revised every 8 years. It is recommended to consider the geographical location and traffic conditions of mountain tunnels when calculating the air demand required for diluting NO2. NOx has basically replaced CO as the primary pollutant with the most significant impact on human health in mountain tunnels. It is recommended that NOX be considered as the primary hygiene control indicator in the ventilation design of mountain tunnels in China. The pollutant distribution and ventilation control indexes of mountain and urban highway tunnels are quite different, which should be treated differently in engineering applications. The research results can provide a significant reference for ventilation design and environmental evaluation of highway tunnels in mountainous areas.