High Content Of Unsaturated Fatty Acids Research Articles

Transcriptome analysis of golden pompano (Trachinotus ovatus) liver indicates a potential regulatory target involved in HUFA uptake and deposition

Promoting highly unsaturated fatty acid (HUFA) uptake and deposition can improve nutritional value of farmed fish and reduce dietary fish oil addition. Previously, we found that the golden pompano Trachinotus ovatus liver HUFA content increased with the increasing of dietary HUFA. Therefore, we examined the common genes and pathways responsible for HUFA uptake and deposition in T. ovatus liver using transcriptome sequencing technology after feeding with either 1.0% or 2.1% HUFA for 8 weeks. Results showed that a total of 140 and 147 genes were significantly upregulated and downregulated, respectively. Five bile acid synthesis-related genes (CYP7A1, CYP8B1, AKR1D1, SCP2 and ACOT8), which are related to dietary fat emulsification were downregulated in 2.1% HUFA group, implying that the cholate synthesized through the classical pathway might be the main bile acid form in fat emulsification. Moreover, fatty acid transport protein (FATP)-6, fatty acid binding protein (FABP)-1, -4, and -6 increased with HUFA deposition, especially FATP6 and FABP4, suggesting that the two genes may be important mediators involved in HUFA uptake and deposition. KEGG analysis showed that most of the differential genes described above were involved in peroxisome proliferator activator receptor (PPAR) signaling pathway, and PPARγ increased with HUFA deposition, indicating that PPARγ might be a key regulator of HUFA uptake and deposition by regulating the genes involved in fatty acid emulsification and transport. This study focused on the liver, which is the center of intermediary metabolism, providing a comprehensive understanding of the molecular regulation of HUFA uptake and deposition in T. ovatus, which should be further investigated to develop potential measures to improve HUFA content.

Perspectives and safety of horsemeat consumption

SummaryIn some countries, use of horsemeat as a food is inhibited by ethical and cultural concerns. However, horsemeat has potential health benefits, such as low fat and high unsaturated fatty acid content compared with other meats, as well as attractive sensory properties. Although there are many contradictory data on the dietary value in relation to human health, the effects of horsemeat have not been studied to a large extent. In this paper, we summarise existing knowledge on horsemeat quality and effects on human health. Our conclusion is that horsemeat may be consumed as a healthy alternative to other types of meat, provided that risks associated with microbial contamination during storage and possible presence of contaminants in horsemeat are taken into consideration. Future studies should evaluate the health‐promoting properties and safety of horsemeat and associated byproducts.

Physico-chemical properties and fatty acid composition of <em>Chrozophora tinctoria</em> seeds as a new oil source

Chrozophora tinctoria L., usually known as dyer’s Croton, Turnsole or Giradol, has been used in various medicinal and food products for many years. However, no comprehensive research has been undertaken to assess its potential as a new seed oil crop. Therefore, the current study examined the fatty acid composition, physico-chemical properties and antioxidant activity of C. tinctoria seeds, grown in the southwest of Iran. The seed oil content was found to be 26.40%. The extracted oil was analyzed for fatty acid composition using gas chromatography (GC). The results showed that unsaturated fatty acids accounted for almost 91% of the total fatty acids. Linoleic acid was the dominant fatty acid (76.68%), followed by oleic acid (13.99%) and palmitic acid (5.32%). δ-tocopherol was the major tocopherol in the oil, representing 70 mg/100 g oil. The total phenolic content (151.70 mg GAE per 100 g oil) and total flavonoid content (1.17 mg QE oil) were also determined in the extracted oil. The antioxidant activity was measured by a DPPH assay and expressed as 45% of the seed oil. Due to its high oil yield and high unsaturated fatty acid content, C. tinctoria could be regarded as a new source of edible oil.

Development of a culture protocol for Rhodomonas sp. Hf-1 strain through laboratory trials

Rhodomonas sp. Hf-1 is a marine microalgal strain with a high nutritional value. In order to produce this microalga effectively and inexpensively in a hatchery, its production protocol was reexamined through several culture trials. Firstly, it was found that the Hf-1 strain required nitrogen, phosphorus, iron, manganese and vitamin B12. The optimal nitrogen source was urea. Based on these results, a new medium, MU–SW, was formulated from agricultural fertilizers. Secondly, MU–SW medium was verified at production scale, in which satisfactory growth of the Hf-1 strain was obtained. The cell density reached approximately 3.0 × 106 cells ml−1, and five harvest cycles at 4-day intervals could be achieved. The high unsaturated fatty acid content of the harvested cells fluctuated in the following ranges: C18:2n-6, 19.8–25.2%; C18:3n-6, 8.7–15.2%; C20:4n-6, 2.0–4.6%; C20:5n-3, 8.2–11.3% and C22:6n-3, 5.6–7.3%. This study provides the baseline conditions for introducing the Hf-1 strain into an aquaculture food chain.

Oxidative Stability and Quality Characteristics of Duck, Chicken, Swine and Bovine Skin Fats Extracted by Pressurized Hot Water Extraction.

The aim of this study was to investigate the oxidative status and quality characteristics of four animal skin-derived fats extracted using an identical extraction method. Pressurized hot water extraction, a green extraction method, was used to extract animal skin fats (duck, chicken, swine, and bovine skin). Multiple experiments were performed during accelerated storage at 60°C for 90 days. Quality characteristics, such as extraction yield, iodine value (IV), fatty acid composition, and fat viscosity were determined. In addition, indicators for oxidative status, including acid value (AV), peroxide value (PV), p-anisidine value (p-AV), thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), and total oxidation (totox) values were evaluated. The fat extraction yield was highest in bovine fat, followed by duck, swine, and chicken fats. The IV was higher in duck and chicken fats. Duck fats contained the most unsaturated fats and the least saturated fats. Fat oxidation indicators, such as PV, TBARS, and totox values, were relatively higher in duck fats during storage compared to the other fats. Other indicators, including AV, p-AV, and CD, were similar in duck, chicken, and swine fats. Viscosity was similar in all the tested fats but markedly increased after 70 days of storage in duck fats. Our data indicate that duck skin fat was more vulnerable to oxidative changes in accelerated storage conditions and this may be due to its higher unsaturated fatty acid content. Supplementation with antioxidants might be a reasonable way to solve the oxidation issue in duck skin fats.

Effect of oil enrichment on Brachionus plicatilis rotifer and first feeding red sea bream (Pagrus major) and Japanese flounder (Paralichthys olivaceus)

Euryhaline rotifer belonging to the genera Brachionus are often used as live feed for first feeding marine finfish larviculture. Two common rotifer enrichments, a single usage of docosahexaenoic acid (DHA)-enriched Chlorella vulgaris and a combination of DHA-enriched C. vulgaris and salmon roe emulsion oil were used to assess: (i) nutritional status of rotifers and, (ii) performance when rotifers were fed to Pagrus major and Paralichthys olivaceus larvae from 2 to 15 days after hatching (DAH). Both rotifer enrichment treatments were found to be nutritionally effective with high unsaturated fatty acid (HUFA) content, especially in the combined treatment with significantly higher eicosapentaenoic acid (EPA). Despite deficient levels of EPA, DHA-enriched C. vulgaris was found to be a suitable enrichment for rotifer and also had positive response in survival, growth and trypsin activity in both P. major and P. olivaceus. DHA-enriched C. vulgaris only treatment had higher rotifer population growth (13% higher), egg bearing capacity (7% higher), almost double the soluble protein content and generally higher free amino acid content. Oxidation of lipids in the combined diet due to its high-saturated fatty acid content could be a possible cause to the inferior performance of the combined treatment. Both P. major (20.1 ± 10.1%) and P. olivaceus (12.3 ± 3.87%) suffered low survival and lower gut content in the combined treatment especially during periods of significant growth in both species (7–11 DAH). Despite high HUFA content in the combined treatment, this study revealed that consideration of free amino acids and soluble protein content as parameters of nutritional indicators for first feeding P. major and P. olivaceus should not be discounted.

Effect of sedimentary organic matter on species richness of deposit feeders in enclosed bay ecosystems: Insight from fatty acid nutritional indicators

How nutritional quality of dietary resources affects species richness of consumer communities is poorly understood. We used fatty acids as indicators of nutritional quality of sedimentary organic matter to evaluate the effects of highly unsaturated fatty acid (HUFA) content in sediments and fatty acid diversity in sedimentary organic matter on species richness of deposit feeders. We sampled benthic animals and sedimentary organic matter, a potential dietary source for deposit feeders, at 54 locations in two bay ecosystems. The species richness of deposit feeders ranged between 1 and 29 and had a parabolic relationship with the organic carbon content of sediments. At intermediate range of sedimentary organic carbon content, the species richness of deposit feeders was positively related to HUFA content and fatty acid diversity. These findings indicate that nutritional quality is one of the important factors determining species richness. In particular, HUFA content and fatty acid diversity are useful indicators of the nutritional quality of potential diets and good predictors of the occurrences of benthic invertebrates in marine habitats.

Egg Yolk Phosphatidylethanolamine: Extraction Optimization, Antioxidative Activity, and Molecular Structure Profiling.

Although phosphatidylethanolamine (PE) is an important functional phospholipid, there have been very few reports on its antioxidant activity and corresponding molecular composition. Crude PE was extracted from egg yolk with various solvents, and response surface modeling was carried out to determine the optimum extraction conditions for PE, under which the PE content in extracts reached 58.94 µg/mL. The crude PE was purified using silica gel-based column chromatography. High-purity PE (98%), identified by high-performance liquid chromatography-evaporative light-scattering detector, was obtained using isocratic elution with a mixed solvent (chloroform: methanol: acetic acid=18:5:1) eluent. PE purified from egg yolk exhibited high radical-scavenging activity, determined by electron paramagnetic resonance (EPR). The result was attributed to the high unsaturated fatty acids (83.10%) content in egg yolk PE, and the unsaturated fatty acids were identified as PE-16:0/18:1Δ 9 , PE-16:0/18:2Δ 9,12 , PE-16:0/20:4Δ 5,8,11,14 , PE-18:0/18:1Δ 9 , PE-18:0/18:2Δ 9,12 , and PE-18:0/20:4Δ 5,8,11,14 by MALDI-TOF MS combined with gas chromatography mass spectrometry. PRACTICAL APPLICATION: In this work, an attempt has been made to explore the antioxidant activity of PE that extracted and purified from egg yolk and its corresponding molecular composition. Owing to its plentiful unsaturated fatty acids (83.10%), purified PE from egg yolk exhibited a high radical-scavenging activity that indicated that egg-yolk PE had a strong antioxidantactivity, and it might exert possible beneficial effects on the human health.

Supercritical CO₂ Fluid Extraction of Elaeagnus mollis Diels Seed Oil and Its Antioxidant Ability.

Supercritical fluid carbon dioxide (SF-CO2) was used to extract oil from Elaeagnus mollis Diels (E. mollis Diels) seed and its antioxidant ability was also investigated. The effect of extraction pressure (20–35 MPa), extraction temperature (35–65 °C), extraction time (90–180 min) and seed particle size (40–100 mesh) on the oil yield were studied. An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield. Based on the optimum conditions, the maximum yield reached 29.35% at 30 MPa, 50 °C, 150 min, 80 mesh seed particle size and 40 g/min SF-CO2 flow rate. The E. mollis Diels seed (EDS) oil obtained under optimal SF-CO2 extraction conditions had higher unsaturated fatty acid content (91.89%), higher vitamin E content (96.24 ± 3.01 mg/100 g) and higher total phytosterols content (364.34 ± 4.86 mg/100 g) than that extracted by Soxhlet extraction (SE) and cold pressing (CP) methods. The antioxidant activity of the EDS oil was measured by DPPH and hydroxyl radical scavenging test. EDS oil extracted by different methods exhibited a dose-dependent antioxidant ability, with IC50 values of no significant differences. Based on the results of correlation between bioactive compounds, lupeol and γ-tocopherol was the most important antioxidant in EDS oil.

Feasibility of unsaturated fatty acid feedstocks as green alternatives in bio‐oil refinery

AbstractBio‐oil contains plant, animal, fish, and micro‐algae oil, and more than 200 million tons are produced annually. However, many edible and non‐edible bio‐oils are still under development, and their value and feasibility have yet to be determined. The vast majority of bio‐oils produced in the world have a very high unsaturated fatty acid content (70–98%), which could be used as a renewable, eco‐friendly alternative to petroleum. Hydrolysis (or transesterification) with ethylene metathesis technology converts unsaturated fatty acids to C4–C30 oil fragments. These oil fragments are a sustainable chemical technology used in chemical industry platform technology and carbon dioxide abatement green chemical technology, and as a petrochemical substitute. These platform chemicals also have their own market but are used as raw materials for other products (polymers, surfactants, synthetic lubricants, plasticizers, other specialty chemicals, general purpose chemicals, biofuels, etc.). The biodegradability and biocompatibility of the polymeric materials made from the oil mean that it is also possible to develop diverse functional products with high added value such as toothpaste, dandruff treatment shampoo, and melanin pigment removal cosmetics, medical and shape memory polymer materials. According to the cradle‐to‐gate analysis for environmental impact and economics, the total carbon dioxide emission reduction per ton of soybean oil was estimated to be 1.5 kg kg−1 products. Moreover, the metathesis of soybean oil ($680/ton) with additional raw materials ($237.4) will generate a value that is 7.5 times higher at $6857. Due to the biodegradability and biocompatibility of the products made from bio‐oils, it is expected that various functional products with high added value will be developed and that industrial applicability will be greatly expanded in the near future. In addition, for the development of various derivatives, fusion and cooperative research in chemical fields such as catalytic engineering, organic chemistry, polymer chemistry, maintenance chemistry, and reaction engineering are essential. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

Dietary protein level and corn processing method: Lactation performance, milk fatty acid composition, rumen and blood parameters of lactation dairy cows

The objective of this experiment was to evaluate the interactive effects of corn processing and dietary protein level on milk production and composition, as well as rumen and plasma metabolites in lactation dairy cows. Eight multiparous mid lactating Holstein cows, averaging 105 ± 9 d in milk and producing 47.2 ± 3 kg milk/d were randomly assigned in a replicated 4 × 4 Latin square with a 2 × 2 factorial arrangement of treatments. Experimental diets contained either finely ground corn (FGC) or steam flaked corn (SFC) each with either low protein (LP, 14.8%) or high protein (HP, 16.2%) content. Data were analyzed using PROC MIXED of SAS with significance declared at P ≤ 0.05. Cows fed HP diets had greater milk production and lower milk fat percentage than those for cows fed LP diets. Cows on LP diets however had greater medium-chain fatty acid (FA) and saturated FA but lower monounsaturated FA, polyunsaturated FA and unsaturated FA compared with cows on HP diets. Reduction of milk fat for cows on HP was associated with lower amount of short and medium chain FA and higher unsaturated FA content in milk fat. Blood glucose (74.7 vs 69.3 mg/dL) and cholesterol (275.2 vs 262.9 mg/dL) were greater for cows fed SFC than cows fed FGC. Corn processing had no detectable effect on milk production; however, cows on FGC had greater milk fat percentage and production and lower milk: feed ratio than cows fed SFC. Cows fed SFC had greater amount of short chain FA and polyunsaturated FA and lower C16:0 (30.5 vs 29.3) and C18:0 (9.1 vs 8.2) in milk fat than cows fed FGC. Treatments had no effect on ruminal VFA concentration and pH. Milk and blood urea, and rumen nitrogen (N) were lower for cows when fed SFC or LP diets. Cows fed SFC or LP had greater N efficiency than cows fed FGC or HP diets. These findings showed that cows supplemented with SFC or fed LP diets had a more efficient use of dietary N. Results indicate that employing a 14.8% CP diet is insufficient for maximum milk production even with corn flaking. Cows fed LP diets had similar milk: feed ratio and greater N efficiency. Compared with FGC diets, SFC diets improved feed and N efficiency while reducing N loss and increasing the glucose concentration.

Characterization, antibacterial activity and antibiotic modifying action of the Caryocar coriaceum Wittm. pulp and almond fixed oil

In this study the physicochemical characterization of the pulp and almond fixed oil was carried out; their antibacterial activity and aminoglycoside antibiotic modifying action against standard and multiresistant Gram-positive and -negative bacteria were investigated using the broth microdilution assay. Physical properties such as moisture, pH, acidity, peroxide index, relative density and refractive index indicate stability and chemical quality of the oils. In the GC/MS chemical composition analysis, a high unsaturated fatty acid content and the presence of oleic and palmitic acids were observed in the oils. In the antibacterial assay, more significant results were obtained for Escherichia coli, while other standard and multi-resistant strains presented MIC values ≥ 1024 μg/mL. Furthermore, the fixed oils in association with antibiotics were able to significantly improve antibacterial activity against S. aureus with a reduction in MICs.

Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds.

Tree peony (Paeonia section Moutan DC.) species are woody oil crops with high unsaturated fatty acid content, including α-linolenic acid (ALA/18:3; >40% of the total fatty acid). Comparative transcriptome analyses were carried out to uncover the underlying mechanisms responsible for high and low ALA content in the developing seeds of P. rockii and P. lutea, respectively. Expression analysis of acyl lipid metabolism genes revealed upregulation of select genes involved in plastidial fatty acid synthesis, acyl editing, desaturation, and triacylglycerol assembly in seeds of P. rockii relative to P. lutea. Also, in association with ALA content in seeds, transcript levels for fatty acid desaturases (SAD, FAD2, and FAD3), which encode enzymes necessary for polyunsaturated fatty acid synthesis, were higher in P. rockii compared to P. lutea. Furthermore, the overexpression of PrFAD2 and PrFAD3 in Arabidopsis increased linoleic and ALA content, respectively, and modulated the final ratio 18:2/18:3 in the seed oil. In conclusion, we identified the key steps and validated the necessary desaturases that contribute to efficient ALA synthesis in a woody oil crop. Together, these results will aid to increase essential fatty acid content in seeds of tree peonies and other crops of agronomic interest.

Aurantiochytrium dietary supplements reduce intra-cohort cannibalism among orange-spotted groupers (Epinephelus coioides) by modulating brain 5-HT and serum cortisol

Aurantiochytrium strain BL10 is rich in highly unsaturated fatty acids (HUFAs), particularly docosahexaenoic acid (DHA). Previous research has shown that adding 2% of dry BL10 powder to increase the DHA content of feed for orange-spotted grouper (Epinephelus coioides) does not benefit growth but can significantly reduce size discrepancies between individuals and thereby potentially decrease the chance of cannibalism.To understand whether a 2% BL10 supplement can really reduce the cannibalism rates of E. coioides, whether increasing the BL10 dosage can reduce the cannibalism rates of E. coioides even further, and how the BL10 supplements affect cannibalistic behavior, we produced four isonitrogenous and isolipidic experimental diets dosed with different weight percentages (0, 2, 4, 6%) BL10 meal and fed it to E. coioides fingerlings (total length 3.8 ± 0.3 cm) for two weeks. During the feeding period, cannibalism in the experiment groups were observed. After the feeding period, we analyzed the brain fatty acid composition, brain 5-hydroxytryptamine (5-HT) levels, and serum cortisol levels in the experiment groups and used regression analysis to examine the relationships among these biochemical variants and cannibalism rates.The results indicate that 2–6% BL10 supplements, particularly 2% BL10 supplement, could significantly reduce the cannibalism rates of E. coioides. The BL10 supplements increased the proportions and contents of HUFAs in the brains of E. coioides, increased brain 5-HT levels, and reduced serum cortisol levels. The results of univariate linear regression indicated that the serum cortisol level exhibited a significant negative correlation with brain HUFA content, particularly arachidonic acid (ArA). Whereas, brain 5-HT was positively correlated with the ratio of n-3/n-6 fatty acids in the brain. Subsequent multivariate linear regression demonstrated that cannibalism is affected by serum cortisol, brain 5-HT levels and their interaction. Our results provide new insight into the physiological regulation mechanisms behind the cannibalistic behavior of teleost and provide a feasible solution at the same time.

Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle.

We have created a new, fast-growing strain of common carp with higher unsaturated fatty acid content in muscle. To better understand the impacts of gene regulation in intestinal tissue on growth and unsaturated fatty acid content, we conducted a comparative RNA-Seq transcriptome analysis between intestine samples of Selected and Control groups (and corroborated selected results by PCR). After eight weeks of cage culture, weight gain of the Selected group was 20.84% higher. In muscles of the control group, monounsaturated fatty acids (FAs) were more abundant, whereas polyunsaturated FAs were more abundant in muscles of the Selected group. In total, we found 106 differentially expressed genes (DEGs) between the two groups. Only the endocytosis pathway was significantly enriched in DEGs, with two upregulated genes: il2rb and ehd1. The latter is involved in the growth hormone/insulin-like growth factor (Gh/Igf) axis, which plays a key role in the regulation of growth in animals. tll2, which is known to be associated with intestinal regeneration, was extremely highly upregulated in both transcriptomic (infinite) and qPCR (610.70) analyses. Two of the upregulated genes are associated with the fatty acid metabolism, several genes are likely to be indicators of heightened transcription levels, several are associated with metabolic and developmental roles, several with neuronal functions (including two with vision), several with the immune system, and two downregulated genes with the development of vasculature. The higher growth rate of the Selected group is likely to be at least partially attributed to increased endocytosis efficiency and genetically-driven behavioural differences (higher aggression levels). There are some indications that this new strain might have slightly impaired immune responses, and a higher propensity for inherited diseases leading to sight impairment, as well for neurodegenerative diseases in general, but these indications still need to be confirmed.

Effects of Stocking Density on Milk Fatty Acids Composition and Oxidative Stability of Mid- and Late-Lactating Dairy Cows.

Simple SummaryThe ratio of unsaturated fatty acids (UFAs)/saturated fatty acids (SFAs) of milk fat is known to play an important role in regulating milk oxidation capacity in lactating dairy cows. Recently, it was found that dietary antioxidant addition plays an important role in changing UFAs/SFAs ratio of milk fat and milk oxidation capacity in dairy cows, indicating the role of oxidative stress status in regulating milk oxidation capacity. Although stocking density can affect stress level in lactating dairy cows, whether stocking density may affect milk oxidation capacity remains to be answered. In the current study, we stocked the cows with different physiological stages (mid- and late-lactating) under different stocking density (100% vs. 75%), respectively. The plasma variables, milk fatty acids compositions, and oxidative index in the milk were further evaluated to clarify if stocking density can regulate the oxidation capacity of the milk. The results showed that, in mid-lactating cows, milk produced by low stocking density animals had lower oxidation stability due to the higher UFAs levels, compared with those of high stocking density cows. Milk oxidation capacity of late-lactating dairy cows did not differ when they were blocked under different stocking density. Our results suggested that stocking density did affect milk oxidation capacity but in a physiological-stage dependent manner. Nutritional strategies should be developed to improve the milk oxidation capacity of cows managed under a low stocking density.The objective of the current study was to investigate the effects of stocking density (SD) on the milk fatty acid profile and oxidation stability in mid- and late-lactating cows. Twenty-four multiparous mid-lactating Holstein dairy cows (milk yield = 34.5 kg/d (standard deviation 0.68), days in milk = 106 (standard deviation 2.2)) and 24 multiparous late-lactating animals (milk yield = 31.8 kg/d (standard deviation 0.98), DIM = 201 (standard deviation 3.5)) were allocated into 12 blocks based on parity, milk yield, and DIM and were randomly assigned to one of four treatments: mid-lactating cows with high SD (HSD, 100%), mid-lactating cows with low SD (LSD, 75%), late-lactating cows with HSD, and late-lactating cows with LSD. The experiment lasted for eight weeks. Lactation performance (milk yield and composition including fat, protein and lactose) was measured weekly. The milk fatty acids (FAs) profiles and oxidation stability indexes in milk were measured in the fourth day of the eighth week. Plasma variables were measured in the fourth day of fourth and eighth experimental weeks. Yield and composition (protein, fat and lactose) were not affected by SD in neither mid- nor late-lactating dairy cows. Among mid-lactating cows, LSD cows had higher contents of unsaturated FAs (total, C18:1 cis-9 and C18:2 cis-9 cis-12) and lower concentrations of saturated FAs (total, C14:0 and C16:0) in milk fat than those of the HSD animals. Moreover, LSD lowered SOD, GSH-px, and T-AOC activities and reduced the malonaldehyde content in the milk of mid-lactating cows compared with those of HSD cows. Mid-lactating cows under LSD had reduced cortisol and greater MDA contents in plasma than those of HSD cows. Our results suggested that the effect of SD on the milk FA profile and stability varied depending on lactation stages. In mid-lactating cows, although cows with LSD were less stressed, the milk they produced had lower oxidation stability due to the higher unsaturated FAs levels compared with that of HSD cows.

The effect of rosemary (Rosmarinus officinalis L.) extract on the oxidative stability of lipids in cow and soy milk enriched with fish oil

Lipid oxidation of fish oil enriched cow milk and soy milk supplemented with rosemary extract stored at 2 °C was studied. Both peroxide value and volatile secondary lipid oxidation products were determined to monitor the progress of lipid oxidation. Rosemary extract inhibited lipid oxidation in fish oil enriched cow milk. In contrast, soy milk samples having much higher unsaturated fatty acid content showed higher lipid oxidation stability compared to cow milk. Reduction in the content of chlorogenic acid during storage suggested that this compound may contribute to the lipid oxidation stability of fish oil enriched soy milk product. Total carnosic acid and carnosol concentration declined much faster in soy milk than in cow milk. It is suggested from the results that food components could have significant impact on the fate of bioactive antioxidant compounds in a specific food product during storage.

Polyunsaturated Fatty Acid Desaturase‐Mediated NAD+ Recycling Permits Ongoing Glycolysis and Cell Proliferation

The delta‐5 desaturase (D5D) and delta‐6 desaturase (D6D), encoded by FADS1 and FADS2 respectively, are required for the synthesis of highly unsaturated fatty acids (HUFAs). Genetic variants associated with FADS1 and FADS2 expression are associated with HUFA content in circulating lipids, as well as fasting glucose, height and weight, and risk of colon and laryngeal cancer. Why genetic variation in HUFA synthesis has such a broad impact on human metabolic and proliferative phenotypes is unknown. Here, we show that HUFA synthesis is a mechanism for cytosolic NAD+ recycling that permits ongoing glycolysis. Consistent with this, lowering the cytosolic NAD+/NADH ratio via inhibition of mitochondrial respiration increased D5D and D6D activity in vitro and in vivo. Conversely, increasing the cytosolic NAD+/NADH ratio via expression of an NADH oxidase or activation of lactate dehydrogenase decreased D5D and D6D activity. Increasing D5D and D6D expression increased cytosolic NAD+/NADH, whereas reduced expression or inhibition of D5D and D6D decreased cytosolic NAD+/NADH and increased lactate production. Inhibition of D5D and D6D also reduced cell proliferation, an effect that was reversed by boosting cytosolic NAD+ but not with HUFA end‐product supplementation. Finally, we show that the type 2 diabetes risk haplotype in SLC16A11, which reduces pyruvate transport, also increases D5D and D6D activity, demonstrating the relevance of our findings to human disease. These results outline a novel link between glycolysis and HUFA desaturation mediated by cytosolic NAD+, and provide new insight on how D5D and D6D activity may impact glycemic, growth, and cancer traits in humans.Support or Funding InformationThis work was supported by NIH U01 DK060990 and the Extramural Grant Program of Satellite Health Care, a not‐for‐profit renal care provider.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Synthesis and characterization of a potential bio-based dielectric fluid from neem oil seed

Vegetable oil with high percentage unsaturated fatty acid content are unstable to oxidation at elevated temperatures. However, epoxidation is an effective chemical reaction for chemical structural modification of such oil to eliminate unsaturated carbon–carbon double bond which is responsible for the instability of the oil. In this research, laboratory purified neem oil was epoxidized in situ in the presence of an acidic catalyst and the product was further transesterified. The purified (PNO), epoxidized (ENO) and transesterified (ENOE) oil samples produced were characterized by FTIR, Viscosity measurement using Brookfield Digital Viscometer, relative permittivity, loss tangent and ac conductivity measurements were made using a coaxial probe placed in a thermostatically controlled heat chamber and a frequency analyzer at varying frequency and temperature in the range 20 Hz to 20 kHz and 20 °C to 70 °C respectively. All three samples show Arrhenius type behavior for viscosity and ac conductivity. ENOE sample had least viscosity of 0.0254 Pa s at 40 °C. Both PNO and ENO had a.c conductivity of the order 10−10 S/m at 20 °C and power frequency and loss tangent of 0.0082 and 0.0191 respectively. The oil samples possess low loss in the temperature and frequency ranges considered and are suggested to be suitable candidate for potential natural ester insulating fluid in High Voltage equipment.

The effect of pyrogallol antioxidant addition and storage temperature to the change of biodiesel quality during storage period

Biodiesel is an alkyl ester compound of fatty acids prepared from a source of naturally renewable triglycerides and used as diesel engine fuel, commonly made through the process of esterification or transesterification. In its application and storage, biodiesel is potentially damaged by oxidation reaction due to internal factors (high unsaturated fatty acid content) and external factors (air, heat or light) resulting in changes of the characteristics and quality of biodiesel. In order to maintain the characteristics and quality of biodiesel to conform to established standards, it is necessary to add antioxidants that can inhibit oxidation reaction in biodiesel. In this study, pyrogallol antioxidant is added to the biodiesel of palm oil with various concentrations and storage temperatures. The observed biodiesel parameters during the storage period are those that can represent oxidation such as changes in kinematic viscosity, density, acid value, and iodine value. The results showed that the addition of pyogallol antioxidant can inhibit the oxidation reaction in biodiesel. The use of antioxidant with a concentration of 0.1% at storage temperatures of 30°C and 60°C is known to retain the characteristics and quality of biodiesel during storage from damage caused by oxidation reaction.