Highest Radical Scavenging Activity Research Articles

L-glycine and l-glutamic acid protect Pediococcus pentosaceus R1 against oxidative damage induced by hydrogen peroxide

The effects of l-glycine (Gly) and l-glutamic acid (Glu) on oxidative damage induced by hydrogen peroxide (H2O2) in Pediococcus pentosaceus R1 were investigated. Gly and Glu significantly reduce the production of intracellular reactive oxygen species and the levels of malondialdehyde and carbonylated proteins and concomitantly increase ATP levels in P. pentosaceus R1 under H2O2-induced stress (P < 0.05). Transmission electron microscopy and atomic force microscopy of bacteria under H2O2-induced stress revealed that Gly and Glu suppress bacterial membrane deformation and cell damage. Gly exhibited stronger ferrous ion-chelating ability, whereas Glu has higher radical scavenging activities and reducing power (P < 0.05). The abilities of Gly and Glu to inhibit lipid peroxidation are comparable. Gly and Glu significantly enhance the activities of superoxide dismutase and glutathione peroxidase, respectively, and increase the total antioxidant capacity of bacteria (P < 0.05). These findings indicate that Gly and Glu alleviate H2O2-induced oxidative stress via direct antioxidant effects and increase the activities of bacterial antioxidant enzyme.

Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties

Impact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.

Green synthesis of magnesium nanoparticles mediated from Rosa floribunda charisma extract and its antioxidant, antiaging and antibiofilm activities

Flower based nanoparticles has gained a special attention as a new sustainable eco-friendly avenue. Rosa floribunda charisma belongs to modern roses with bright yellow, red flowers with marvellous rose scent. Different methods were used for the extraction of its floral scent such as hexane, microwave, and solid-phase micro-extraction. The latter was the most efficient method for the extraction of phenyl ethyl alcohol, the unique scent of roses. In the current study, magnesium nanoparticles (RcNps) have been synthesized using Rosa floribunda charisma petals that have privileges beyond chemical and physical routs. RcNps formation was confirmed using UV–Visible (UV–Vis) Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), High Resolution-Transmission Electron Microscope (HR-TEM), Field Emission-Scanning Electron Microscope (FE-SEM), Energy dispersive X-ray (EDX), X-ray Diffractometer (XRD), and X-ray photoelectron spectroscopy (XPS). HR-TEM images detected the polyhedral shape of RcNps with a diverse size ranged within 35.25–55.14 nm. The resulting RcNps exhibited a high radical scavenging activity illustrated by inhibition of superoxide, nitric oxide, hydroxyl radical and xanthine oxidase by by IC50 values 26.2, 52.9, 31.9 and 15.9 µg/ml respectively as compared to ascorbic acid. Furthermore, RcNps at concentration of 100 µg/ml significantly reduced xanthine oxidase activity (15.9 ± 0.61 µg/ml) compared with ascorbic acid (12.80 ± 0.32 µg/ml) with p < 0.05. Moreover, RcNps showed an excellent antiaging activity demonstrated by inhibition of collagenase, elastase, hyaluronidase and tyrosinase enzymes in a dose-dependent manner with IC50 values of 58.7 ± 1.66 µg/ml, 82.5 ± 2.93 µg/ml, 191.4 ± 5.68 µg/ml and 158.6 ± 5.20 µg/ml as compared to EGCG respectively. RcNps also, exhibited a promising antibacterial activity against three skin pathogens delineate a significant threat to a public health, as Staphylococcus epidermidis, Streptococcus pyogenes, and Pseudomonas aeruginosa with MIC of 15.63, 7.81, 31.25 µg/ml as compared to ciprofloxacin (7.81, 3.9 and 15.63 µg/ml). Moreover, RcNps suppressed the formation of biofilms with minimum biofilm inhibitory concentrations 1.95, 1.95, 7.81 µg/ml against the fore mentioned strains, respectively. Overall, our findings indicate that Rosa floribunda nanoparticles could be used as a leading natural source in skin care cosmetic industry.

Formulation design, optimization and in vivo evaluation of oral co-encapsulated resveratrol-humic acid colloidal polymeric nanocarriers

The study aims at formulation and optimization of resveratrol and humic acid co-encapsulated colloidal polymeric nanocarriers to improve stability, oral bioavailability, and antiradical activity of water-insoluble, resveratrol. The eudragit E100 polymeric material was used to fabricate resveratrol and humic acid co-encapsulated oral colloidal polymeric nanocarriers (Res-HA-co-CPNs) using emulsification-diffusion-evaporation method. Taguchi orthogonal array design was employed to check the effect of formulation factors on in vitro physicochemical characteristics. The optimized formulation was further evaluated for oral bioavailability as well as for antiradical potential. Optimized Res-HA-co-CPNs demonstrated spherical and smooth surface including mean particle size, 120.56 ± 18.8 nm; polydispersity index, 0.122; zeta potential, +38.25 mV; and entrapment efficiency, 82.37 ± 1.49%. Solid-state characterization confirmed the amorphous characteristic of optimized Res-HA-co-CPNs. In vitro release profile of Res-HA-co-CPNs showed sustained release behavior up to 48 h and CPNs were found to remain stable at the refrigerated condition for 6 months. In vivo pharmacokinetic studies revealed significant (p < 0.05) improvement of ∼62.76-fold in oral bioavailability. The radical-scavenging activity was found to be increased with time and after 72 h, it was analogous to pure Res. IC50 values were reported to be decreased with time. Henceforth, developed Res-HA-co-CPNs was proven to be a proficient dosage form to increase stability, oral bioavailability, and antiradical activity of resveratrol. Highlights Resveratrol-humic acid co-encapsulated colloidal polymeric nanocarriers (Res-HA-co-CPNs) were fabricated by emulsification-diffusion-evaporation method and optimized by Taguchi orthogonal array design. The Res-HA-co-CPNs revealed favorable mean particle size and percent encapsulation efficiency with a spherical and smooth surface. The Res-HA-co-CPNs showed diffusion-controlled release of Res and were found to be stable at the refrigerated condition for 6 months. The optimized Res-HA-co-CPNs demonstrated significantly (p < 0.05) higher oral bioavailability with respect to pure Res and PM. The optimized Res-HA-co-CPNs demonstrated higher radical-scavenging activity with respect to time.

Fullerenol@nano-montmorillonite nanocomposite as an efficient radioprotective agent for ameliorating radioactive duodenal injury

Duodenal injury caused by radiotherapy of upper tumor is a serious and inescapable complication due to the special anatomical structure and complex intestinal microenvironment of duodenum. However, the prevention and treatment of radiation-induced damage of duodenum are still a challenge in clinic. In this study, fullerenol@nano-montmorillonite (FNMT) nanocomposite was obtained by mechanical synthesis which processes good chemical stability, long retention time in duodenum and high free radical scavenging activity. And for the first time we explore its role in protecting duodenum damage caused by X-ray irradiation. On the one hand, fullerenol, with good water solubility, chemical stability, and extensive free radical scavenging ability, can effectively alleviate mitochondrial and DNA damage caused by radiation-generated reactive oxygen species (ROS). On the other hand, nano-montmorillonite (NMMT) is used as a drug carrier, which enables the long residence time of fullerenol in duodenum because of its strong intestinal adhesion ability. By taking advantage of both fullerenol and NMMT, the FNMT nanocomposite significantly reduces radiation-induced diarrhea, weight loss, and duodenum tissue pathological damage of mice. This study not only establishes a new platform for duodenal radioprotective agent based on nanomaterials but also has a potential for clinical transformation.

Extracts from Euphorbia heterophylla naturally grown in Brazil – Chemical constitution and bioactivities

Euphorbia heterophylla L. is a herb native of South America and used in traditional African medicine. Although Brazil is considered a center of E. heterophylla dispersion, studies on the medicinal properties of specimens collected in Brazil are lacking. Besides, knowledge about the chemical constituents responsible for the bioactivities of E. heterophylla is limited. Here, we provide the first report on the medicinal properties and chemical composition of bioactive extracts from E. heterophylla naturally grown in Brazil. For this, extracts were obtained by maceration, and hexane extract from leaves was fractionated by gas chromatography. Methanol extracts were partitioned to produce an acid-rich fraction. Extracts and fractions were subjected to spectrometric and spectroscopic analysis, and their antibacterial, radical scavenging, and anti-acetylcholinesterase activities were evaluated. The acid-rich fraction from leaves inhibited Enterococus faecalis and Staphylococcus aureus at 70 and 80 mg mL−1, respectively, and presented high radical scavenging activity (higher than at all the tested concentrations of BHT). The acid-rich fraction from roots (400 μg mL−1) reduced 32.5% of the acetylcholinesterase activity, a key enzyme for the pathogenesis of Alzheimer's disease. Pentacyclic triterpenes were identified as major constituents of the hexane extracts. The acid-rich fraction contained a series of phenolics and fatty acids. These findings corroborate the popular use of extracts from E. heterophylla in traditional medicine and contribute to the understanding of the chemical constituents underlying the therapeutic activities of E. heterophylla.

Total phenolic content and antioxidant activity of limestone endemic Araceae species, Alocasia farisii

The changing environments are giving a rise to free radical, causing development of degenerative disease. A search for natural antioxidant is required as the synthetic antioxidant reported has carcinogenic effects on living organisms. Therefore, the aim of this study is to determine the total phenolic content and antioxidant activity of Alocasia farisii leaves and petioles using three different polarity solvent which are methanol, ethanol and ethyl acetate. The total phenolic content was evaluated using the Folin-Cioucalteu reagent with some modification and the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay. The methanolic extract attained the highest total phenolic content and antioxidant activity at 46.615 μg GAE/g and 66.43 %, respectively. Ethyl acetate with the lowest polarity had the lowest value, 34.769 μg GAE/g total phenolic content and 58.274 % in antioxidant activity. The IC50 value shows methanol recorded the lowest value at 339.905 μg/mL, indicates high radical scavenging activity whereas ethyl acetate has highest IC50 value (400 μg/mL) indicates low radical scavenging. These finding provide useful information on the total phenolic content and antioxidant activity of A. farisii that can be a reference for further research on this species of Araceae family. The leaves and petiole extracts of A. farisii may be exploited as natural sources of antioxidant.

Chemical composition and biological activities of aqueous extracts and their sulfated derivatives of pea peel (Pisum sativum L.)

Agro-industrial wastes are an important natural reservoir of bioactive compounds that could be used to obtain useful by-products. The objective of our study was to evaluate the anticoagulation, fibrinolytic, antioxidant, antiproliferative and prebiotic activity of six aqueous extracts of P. sativum waste and their sulfated derivatives, with particular attention to the content of certain bioactive compounds. Three sulfated extracts exhibited significant anticoagulation activity at 2 mg/ml. Alkaline insoluble sulfated extract showed the highest anticoagulation activity at a concentration of 800 μg/ml, which had a clotting time of 50.11 min. All aqueous extracts exhibited high free radical scavenging activity using DPPH (ranging from 91 to 98.6%) than sulfated ones. They had also good prebiotic properties as they promoted the growth of the two tested probiotics up to 260%. All extracts exhibited also relevant in vitro antiproliferative activity using MTT assay. Sulfated alkaline soluble and insoluble extracts had potent activities against HepG-2 cancer cell lines (55.8 and 52.8%, respectively) compared to Doxorubicin® (52.6%), while sulfated acidic insoluble, neutral soluble and alkaline soluble extracts had potent activities against MCF-7 cancer cell lines (73.4, 72.4 and 72.8%, respectively) compared to Doxorubicin® (72%) with low IC50 values. To author's knowledge, this is the first report investigating the anticoagulation activity of isolated pea peel extracts.

Towards a Bioactive Food Packaging: Poly(Lactic Acid) Surface Functionalized by Chitosan Coating Embedding Clove and Argan Oils.

Here we introduce a new method aiming the immobilization of bioactive principles onto polymeric substrates, combining a surface activation and emulsion entrapment approach. Natural products with antimicrobial/antioxidant properties (essential oil from Syzygium aromaticum—clove and vegetal oil from Argania spinosa L—argan) were stabilized in emulsions with chitosan, a natural biodegradable polymer that has antimicrobial activity. The emulsions were laid on poly(lactic acid) (PLA), a synthetic biodegradable plastic from renewable resources, which was previously activated by plasma treatment. Bioactive materials were obtained, with low permeability for oxygen, high radical scavenging activity and strong inhibition of growth for Listeria monocytogenes, Salmonella Typhimurium and Escherichia coli bacteria. Clove oil was better dispersed in a more stable emulsion (no separation after six months) compared with argan oil. This leads to a compact and finely structured coating, with better overall properties. While both clove and argan oils are highly hydrophobic, the coatings showed increased hydrophilicity, especially for argan, due to preferential interactions with different functional groups in chitosan. The PLA films coated with oil-loaded chitosan showed promising results in retarding the food spoilage of meat, and especially cheese. Argan, and in particular, clove oil offered good UV protection, suitable for sterilization purposes. Therefore, using the emulsion stabilization of bioactive principles and immobilization onto plasma activated polymeric surfaces we obtained a bioactive material that combines the physical properties and the biodegradability of PLA with the antibacterial activity of chitosan and the antioxidant function of vegetal oils. This prevents microbial growth and food oxidation and could open new perspectives in the field of food packaging materials.

Exopolysaccharides produced by Pediococcus acidilactici MT41-11 isolated from camel milk: Structural characteristics and bioactive properties

In this study, the chemical structure and bioactive properties of the EPS of Pediococcus acidilactici MT41-11 isolated from camel milk were investigated. Two polysaccharide fractions (EPS-1, EPS-2) with molecular weights about 69.0 kDa were obtained, which were purified using DEAE-Sepharose and Sephadex G-100 chromatography. Based on monosaccharide composition, FT-IR, and 1D, 2D NMR spectra, concluded that EPS-1 had a backbone composed of →2)-α-d-Manp-(1→, →3)-α-d-Manp-(1→ and with branches containing α-d-Manp-(1→, EPS-2 had a backbone composed of →6)-β-d-Glcp-(1→, and with branches containing →2)-α-l-Fucp-(1→, →3)-α-d-Glcp-(1→, →2)-α-d-Glcp-(1→, β-d-Glcp-(1→, and α-d-Glcp-(1→. Remarkably, in vitro assays showed that EPS possessed multiple bioactive properties, including stimulating Lactobacillus growth and a high DPPH free radical scavenging activity. Also, it has a good ability to anti-biofilms. Overall, the analysis of all data showed EPS from P. acidilactici MT41–11 can be used as anti-oxidant, anti-biofilm agent, and also as a potential candidate prebiotic for health food or medicine industry.

Polyphenols profile and antioxidant capacity of selected medicinal plants of Bangladesh

Polyphenols in many plant foods have received a great deal of attention due to their biological potency and postulated protective role against certain non-communicable diseases like cancer, neurodegenerative disease, and cardiovascular disease. The current study focuses on 15 indigenous plants which are traditionally being used for medicinal and other purposes in Bangladesh. Specifically, this study aimed to determine the total phenolic content (TPC), profile the polyphenolic composition, and assess the antioxidant capacity (AC) of water and methanol extracts derived from these plants. The TPC was estimated using the Folin-Ciocalteu method, and DPPH inhibition was used to assess the AC. HPLC-centered simultaneous determination method of polyphenols was employed to identify and quantify individual polyphenolic compounds. TPC content (expressed as mg GAE/100 g FW) ranged from 15.5 (Zanthoxylum rhetsa) to 276.9 (Moringa oleifera). The lowest IC50 in the water and methanol extracts were shown by Moringa oleifera and Zanthoxylum rhetsa respectively and they also exhibited high free radical scavenging activity in the corresponding solvents (91.9% and 92.9% DPPH inhibition by Moringa oleifera and Zanthoxylum rhetsa respectively). The lowest DPPH inhibition was observed in Alternanthera philoxeroides (29.8%) and Spilanthes calva (24.5%) in the water and methanol extracts respectively. Ten polyphenols (coumaric acid, chlorogenic acid, caffeic acid, apigenin, apigenin-7-O-neohesperidoside, quercetin-3-β-D-glucoside, luteolin, quercetin-3-O-β-D-glucopyranoside, isorhamnetin and kaempferol) were initially identified and subsequently quantified in the plant extracts. Spilanthes calva, Moringa oleifera, Oxalis corniculata, and Piper retrofractum contained the highest amount of coumaric acid and chlorogenic acid, apigenin, caffeic acid and apigenin-7-O-neohesperidoside respectively. Senna tora had the greatest amount of kaempferol, quercetin-3-β-D-glucoside, and isorhamnetin whereas luteolin and quercetin-3-O-β-D-glucopyranoside were observed to be the maximum in Alternanthera sessilis. To conclude, Moringa oleifera and Senna tora with high percentage of DPPH inhibition and low IC50 were the most potent antioxidant sources among the analyzed samples.&#x0D; Bioresearch Commu. 7(1): 947-954, 2021 (January)

Optimization of ultrasonic-assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study.

The present study discussed the optimization of the ultrasonic-assisted extraction of polysaccharides from daylily polysaccharides (DPs). The extracted crude polysaccharides were further separated and purified, and the antioxidant activities including 1,1-diphenyl-2-111 picrylhydrazyl (DPPH) radical scavenging, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical scavenging, hydroxyl radical scavenging, and ferric-reducing antioxidant power (FRAP) activities of the obtained fractions were also evaluated. The results showed that the optimal ultrasonic-assisted extraction parameters with DPs yield of 15.25 ± 1.13% were water to powder ratio of 25 ml/g, extraction power of 694 W, extraction temperature of 71°C, extraction time of 38 min, and three times extraction. By DEAE Sepharose Fast Flow column, four water-soluble polysaccharide fractions (DP-1, DP-2, DP-3, and DP-4) were successfully obtained. Monosaccharide component analysis showed that the four obtained fractions were all hetero-polysaccharides that mainly contained rhamnose, arabinose, fructose, galactose, glucose, galacturonic acid, and glucuronic acid in different molar ratios. All the four DP fractions did show obvious antioxidant activities in vitro, and the DP-3 component had relatively high ABTS free radical scavenging activity. Overall, our research showed that DPs could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics. PRACTICAL APPLICATION: This article deals with the optimization of the ultrasonic-assisted extraction of polysaccharides from daylily and its antioxidant activities. The results showed that the optimal ultrasonic-assisted extraction yield of DPs was 15.25 ± 1.13%. By DEAE Sepharose Fast Flow column, four water-soluble polysaccharide fractions were successfully obtained, and all the four DP fractions did show obvious antioxidant activities in vitro. Daylily polysaccharides could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics.

Evaluation of Functional Chemical Components and Radical Scavenging Activity in 11 Fermented Fish Products from Thailand

ABSTRACT Fermented foods are produced as a result of a preservation process that adds value to the final food product. This study aimed to evaluate the chemical components and radical scavenging activity of 11 fermented fish products from Thailand. Lipids extracts were used for the analysis of fatty acids, tocopherol, and radical scavenging activity, and water-soluble extracts were used for the analysis of free amino acids, inosine 5′-monophosphate (IMP), organic acids, and radical scavenging activity. The results showed that nam pla contained high levels of free amino acids and IMP; tai pla contained high levels of free amino acids and IMP; and kapi contained high levels of n – 3 fatty acids, α-tocopherol, free amino acids, and IMP. These three fermented foods also had higher radical scavenging activities than the other tested fermented foods. Furthermore, a strong correlation between chemical component and radical scavenging activity also occurs by principal component analysis.

Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line.

Euterpe oleracea Mart. (açai) is a native palm from the Amazon region. There are various chemical constituents of açai with bioactive properties. This study aimed to evaluate the chemical composition and cytotoxic effects of açai seed extract on breast cancer cell line (MCF-7). Global Natural Products Social Molecular Networking (GNPS) was applied to identify chemical compounds present in açai seed extract. LC-MS/MS and molecular networking were employed to detect the phenolic compounds of açai. The antioxidant activity of açai seed extract was measured by DPPH assay. MCF-7 breast cancer cell line viability was evaluated by MTT assay. Cell death was evaluated by flow cytometry and time-lapse microscopy. Autophagy was evaluated by orange acridin immunofluorescence assay. Reactive oxygen species (ROS) production was evaluated by DAF assay. From the molecular networking, fifteen compounds were identified, mainly phenolic compounds. The açai seed extract showed cytotoxic effects against MCF-7, induced morphologic changes in the cell line by autophagy and increased the ROS production pathway. The present study suggests that açai seed extract has a high cytotoxic capacity and may induce autophagy by increasing ROS production in breast cancer. Apart from its antioxidant activity, flavonoids with high radical scavenging activity present in açai also generated NO (nitric oxide), contributing to its cytotoxic effect and autophagy induction.

Evaluation of Phyto Chemical Biochemical and In Vitro Antioxidant Potential of Angelica Glauca Grown at High Altitude Areas of Western Himalayas

Angelica glauca Edgew is an important medicinal and aromatic herb (family Apiaceae). The roots of A. glauca commonly used as spices by local peoples. In the present study, the phytochemical constituents, biochemical parameters and, in-vitro antioxidant activity of A. glauca roots collected from the Himalayan region have been studied. For preliminary phytochemical analysis, the hydroalcoholic and aqueous root extract of Angelica glauca were screened for the presence of carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The biochemical parameter and in-vitro antioxidant potential of Angelica glauca were analysed by using standard methods. Phytochemical analysis of extract showed major classes of phytochemicals constituents such as carbohydrates, protein, alkaloids, glycosides, sterols, triterpenes, saponin, tannins, phenols, flavonoids, and coumarin. The results from the current study demonstrated that A.glauca roots contained carbohydrate (21±0.72 %), crude protein (12.7±0.31 %), total ash (3.86±0.034 %), dietary fiber (18.9±0.14 %), total fat (4.5±0.38 %) and ascorbic acid (68.5±0.19mg/100g). The hydroalcoholic extract showed the highest quantity of total phenol, total flavonoids, and total tannin content compared to aqueous extract. The hydroalcoholic extract exhibited high DPPH radical scavenging activity (IC50=68.1±0.34 µg/ml). The result showed that A.glauca roots have high nutritional and antioxidant potential. Hence the plant can be used as a nutraceutical and natural antioxidant.

Development of biscuits supplemented with papaya seed and peel: effects on physicochemical properties, bioactive compounds, in vitro absorption capacities and starch digestibility.

In this study, wheat biscuits were prepared by supplementing with papaya seed (PS) and papaya peel (PP) in range of 2 to 10%. As compared to control (un-supplemented) samples, PS and PP-supplemented biscuits were analyzed for their physicochemical properties, bioactive compounds, antioxidant activities, in vitro absorption capacities, starch digestibility and sensory attributes. As compared to PS, PP had lower energy value, higher bioactive compounds and antioxidant activity along with better in vitro adsorption capacities. Overall, PS and PP addition from 2 to 10% significantly improved nutritional components, polyphenol compounds, antioxidant activities, and caused marked rises in NO2 - ion, cholesterol and bile acid absorption capacities of supplemented biscuits. Moreover, PS and PP-addition showed significant concentration-dependent decreases in glucose release response during various in vitro starch digestion intervals. Based on sensory characteristics, biscuits supplemented up to 4% PS and PP showed excellent overall acceptability, however, in comparison with PS, 4% PP-supplemented biscuits exhibited the better sensory properties. Therefore, PP-supplemented biscuits up to 4% could be utilized as a convenience food. Moreover, PS and PP-supplementation of wheat biscuits led to improvement of functional properties as a valuable source of bioactive compounds and high radical-scavenging activities.

Identification and quantification of phenolic and volatile constituents in five different Anatolian thyme species using LC–MS/MS and GC-MS, with biological activities

Being widely used in the food industry, thyme is one of the most valuable plants in many aspects, and Turkey is one of the hotspots for thyme industry. Five thyme species, Thymus convolutus (endemic), Thymus fallax, Thymus haussknechtii Velen (endemic), Thymus kotschyanus var. kotschyanus, and Thymus sipyleus from Turkey were subjected in this study. Their essential oils and the extracts of aerial and root parts were obtained. GC-MS analyses showed that camphor was the major compound in T. convolutus (12.7%), and in T. sipyleus (13.1%) essential oils. The major volatile compound of T. fallax was bicyclogermacrene (21.5%), whereas the T. kotschyanus var. kotschyanus essential oil showed a predominance of carvacrol (48.5%), and thymol (22.5%). Carvacrol was also the major compound of T. haussknechtii with 28.2%. LC-MS/MS analyses showed that nicotiflorin (293.28–4201.15 μg/mL), caffeic (516.41–851.28 μg/mL), quinic (344.37–4410.94 μg/mL), rosmarinic (1800.18–9631.71 μg/mL), and fumaric acid (350.88–682.10 μg/mL) were detected as the main compounds in the extracts. All extracts exerted strong antioxidant activity, and the highest radical scavenging activity was demonstrated by T. haussknechtii root extract. T. convolutus and T. haussknechtii aerial parts extracts showed significant antimicrobial activity against C. tropicalis at MIC of 19.53 μg/mL. The extracts were found to be non-toxic against COLO205 and KM12 human colon, and UO-31, and A498 human renal cell lines. Their enzyme inhibitory activities were also evaluated against cholinesterase, tyrosinase and urease enzymes and T. fallax aerial part extract was the most effective with 34.48 ± 0.60% in antibutyrylcholinesterase activity. These species with strong antioxidant and antifungal properties and no toxicity could be beneficial to be used as preservatives in the food processing industry.

Preliminary Investigation on the Physicochemical and Functional Properties of Commercial Salmorejo Found in Spanish Supermarkets.

Salmorejo is a traditional Spanish food made of raw tomatoes, bread, garlic, and virgin olive oil. The food industry aims to satisfy consumer demand for ready-to-eat salmorejo while trying to maintain characteristics of the homemade product. In this work, we have assessed physical-chemical and color parameters, radical scavenging activity, and total polyphenol, lycopene, and β-carotene contents of raw and pasteurized commercial salmorejo, as well as homemade (raw) salmorejo samples. Our results showed that heat treatment had a significant influence on color parameters of salmorejo, with pasteurized samples being less red and exhibiting an increase in the degree of browning. Pasteurized samples also showed the highest radical scavenging activity when expressing the results per dry weight. However, when water content was considered, radical scavenging activity was superior in homemade samples when analyzing non-polar molecules extracted with acetone. Results were similar for polyphenol content. It was also observed that heat treatment affected lycopene but not β-carotene content. When acquiring commercial salmorejo at the supermarket, consumers have the option to choose between already prepared pasteurized or raw salmorejo. According to the results obtained in this work, physicochemical and functional properties of commercial raw salmorejo were comparable to a larger extent than pasteurized salmorejo compared to those exhibited by homemade salmorejo samples.

Fingerprinting profile of flavonol glycosides from Bassia eriophora using negative electrospray ionization, computational studies and their antioxidant activities

A total of twenty-eight flavonol glycosides and one alkaloidal metabolite have been detected in 40% MeOH portion after eluting the crude extract from the aerial parts of Bassia eriophora on C-18 short column. These compounds were tentatively detected by using LC–ESI-MS/MS in negative ion mode [M–H]– and represented two major categories: acylated and non-acylated flavonol glycosides. Obtained data indicated that Bassia eriophora is a rich new source of flavonol glycosides. Selected compounds were studied by DFT and TD-DFT calculations to determine their physico-chemical properties and their antioxidant activity. B. eriophora exhibited high TAC, RP and DPPH radical scavenging activity with IC50 of 177.8 µg/mL. Moreover, reducing power at 5 µg/ml and TAC at 5 µg/ml of B. eriophora showed a positive correlation with total flavonoids. The present results may open an avenue to exploit B. eriophora in pharmaceutical applications.

Therapeutic Effects of Dipterocarpus tuberculatus with High Antioxidative Activity Against UV-Induced Photoaging of NHDF Cells and Nude Mice.

To investigate the therapeutic effects of methanol extracts of Dipterocarpus tuberculatus Roxb. (MED) against UV-induced photoaging, we assessed for alterations in the antioxidant activity, anti-apoptotic effects, ECM modulation, skin appearances, and anti-inflammatory response in normal human dermal fibroblast (NHDF) cells and nude mice orally treated with MED. High levels of tannin content and high free radical scavenging activity to DPPH were determined in MED, while seven active components, namely, gallic acid, bergenin, ellagic acid, ε-viniferin, asiatic acid, oleanolic acid, and 2α-hydroxyursolic acid, were identified using LC–MS analyses. UV-induced alterations in the NO concentration, SOD activity, and Nrf2 expression were remarkably recovered in MED-treated NHDF cells. Moreover, the decreased number of apoptotic cells and G2/M phase arrest were observed in the UV + MED-treated groups. Similar recoveries were detected for β-galactosidase, MMP-2/9 expression, and intracellular elastase activity. Furthermore, MED treatment induced suppression of the COX-2-induced iNOS mediated pathway, expression of inflammatory cytokines, and inflammasome activation in UV-radiated NHDF cells. The anti-photoaging effects observed in NHDF cells were subsequently evaluated and validated in UV + MED-treated nude mice through skin phenotypes and histopathological structure analyses. Taken together, these results indicate that MED exerts therapeutic effects against UV-induced photoaging and has the potential for future development as a treatment for photoaging.