High Response Research Articles

Unlocking (re)purchase potential through corporate responsiveness on social networks: The role of perceived customer orientation

Social media marketing offers companies a new opportunity to connect with consumers through customer interactions. However, resource constraints may prevent companies from responding to every consumer comment. In light of this, the “reply to random comments strategy” emerges as an innovative approach where companies choose to respond to a mix of positive and negative comments. Yet, there is a lack of research and no standard practice for this strategy. This research used a mixed-method approach and signaling theory as a theoretical framework to provide extensive insights into how consumers react to various corporate response rates to random comments on social networks. Qualitative insights were gained through focus group discussions, while an empirical investigation was conducted using least squares modeling. The findings highlight the advantages of a high corporate response rate and the importance of signaling a strong customer orientation to enhance (re)purchase intentions of commenting and observing social network users. They also reveal the crucial mediating role of perceived customer orientation as a novel variable in this context. This research provides social media managers with guidelines on enhancing social network users' (re)purchase intentions by implementing a customer-oriented response strategy. The insights help practitioners build and maintain customer relationships, contributing to a company’s long-term success.

Three odorant-binding proteins of small hive beetles, Aethina tumida, participate in the response of bee colony volatiles

Aethina tumida (small hive beetle, SHB) is a rapidly spreading invasive parasite of bee colonies. The olfactory system plays a key role in insect behavior, and odorant-binding proteins (OBPs) are involved in the first step of the olfactory signal transduction pathway and the detection of host volatiles. However, the olfactory mechanism of OBPs in SHB-localized bee colonies is unclear. In this study, electroantennogram (EAG) and behavioral bioassay showed that only three compounds (2-heptanone, ocimene, and ethyl palmitate) from bee colonies triggered high electrophysiological and behavioral responses. Three antenna-specific OBP genes (OBP6, OBP11, and OBP19) were identified, and they were significantly expressed on adult days 6–7. Furthermore, by combining RNA interference (RNAi) with EAG, olfactometer bioassay, competitive fluorescence binding assays, and molecular docking, we found that these three OBP genes were involved in the recognition of 2-heptanone and ethyl palmitate, and AtumOBP6 is also involved in the recognition of ocimene. These data indicate that AtumOBP6, AtumOBP11, and AtumOBP19 play an important role in the olfactory response to bee colony volatiles. Our results provide new insights into the functions of the OBP families in A. tumida and help to explore more potential target genes for environmentally friendly pest control strategies.

MOF-derived Mo-doped Co3O4: A hierarchical yeast-like structure for superior carbon monoxide sensing

Carbon monoxide (CO) is one of the most dangerous gases owing to its dual threat, role in global warming, and severe impact on human health. Metal-organic framework (MOF) gas-sensing materials have drawn the interest of many different sectors due to their unique structural characteristics. This study used a solvothermal technique and subsequent annealing to create a hierarchical, yeast-shaped Mo-Co3O4 material from a Co-MOF precursor. The prepared materials have been employed in the development of a CO-detection gas sensor. Gas-sensing experiments revealed that Mo-Co3O4 exhibited significantly improved sensing capabilities compared to pure Co3O4. Notably, at 200 °C, 2 mol% Mo-Co3O4 showed high response levels of about 136 at 100 ppm CO concentrations, approximately 50.4 times higher than pure Co3O4. Furthermore, the Mo-doped material exhibited a low detection threshold, excellent reproducibility, long-term stability, good selectivity, and rapid response and recovery times (78.5/55.3 s). Introducing Mo dopants into the Co3O4 material and the hierarchical yeast-like nanostructure are responsible for these improvements in gas-sensing capability.

Development of a Fast Positioning Platform with a Large Stroke Based on a Piezoelectric Actuator for Precision Machining.

In this paper, a fast positioning platform (FPP) is proposed, able to meet simultaneously the requirements of large stroke and high frequency response, developed based on a PZT (piezoelectric actuator) and a quad-parallel flexible mechanism, for application in precision machining. The FPP is driven by a high-stiffness PZT and guided by a flexible hinge-based mechanism with a quad-parallel flexible hinge. The proposed quad-parallel flexible hinge mechanism can provide excellent planar motion capability with high stiffness and good guiding performance, thus guaranteeing outstanding dynamics characteristics. The mechanical model was established, the input and output characteristics of the FPP were analyzed, and the working range (output displacement and frequency) of the FPP was determined. Based on the mechanical model and the input and output characteristics of the FPP, the design method is described for of the proposed FPP, which is capable of achieving a large stroke while responding at a high frequency. The characteristics of the FPP were investigated using finite element analysis (FEA). Experiments were conducted to examine the performance of the FPP; the natural frequency of the FPP was 1315.6 Hz, while the maximum output displacement and the motion resolution of the FPP in a static state were 53.13 μm and 5 nm, respectively. Step response testing showed that under a step magnitude of 50 μm, the stabilization times for the falling and rising edges of the moving platform were 37 ms and 26 ms, respectively. The tracking errors were about ±1.96 μm and ±0.59 μm when the amplitude and frequency of the signal were 50 μm, 50 Hz and 10 μm, 200 Hz, respectively. The FPP showed excellent performance in terms of fast response and output displacement. The cutting test results indicated that compared with the uncontrolled condition, the values of surface roughness under controlled conditions decreased by 23.9% and 12.7% when the cutting depths were 5 μm and 10 μm, respectively. The developed FPP device has excellent precision machining performance.

Synthesis of porous SnO2@Fe2O3 core-shell triple-shelled boxes with selective and rapid response towards HCHO

Heterogenous SnO2@Fe2O3 core-shell triple-layer boxes (TBs) are successfully prepared through using a combination of room-temperature hydrolyzation and annealing treatment on SnO2 double-layer boxes (DBs). The crystallinity, morphology, and microstructure of as-prepared SnO2@Fe2O3 TBs are detailedly characterized. Compared with those of referenced pristine SnO2 or Fe2O3 nanostructures, the overall cubic configuration of heterogeneous SnO2@Fe2O3 with hollow interiors retains largely unaltered. Meanwhile, sensor based on SnO2@Fe2O3 samples synthesized with Fe2O3/SnO2 mole ratio of 1 exhibit superior response, excellent selectivity, shortened response-recovery time, and good long-time stability for formaldehyde (HCHO). It has the highest response (11.72) with the response/recovery time of 4 s/19 s to 50 ppm HCHO, respectively, and a low detection limit (1.07 to 200 ppb) for targeted HCHO under the optimal operating temperature (220 °C). The high absorption ability based on unique structural merits of hierarchically SnO2/Fe2O3 subunit-assembled porous multilayers and synergistic effect of SnO2/Fe2O3 heterojunction may contribute to their excellent HCHO sensing performance.

Biobased, degradable and directional porous carboxymethyl chitosan/lignosulfonate sodium aerogel-based piezoresistive pressure sensor with dual-conductive network for human motion detection

The rapid growth of wearable electronics, healthcare monitoring, and human–computer interaction has sparked growing interest in flexible piezoresistive pressure sensors, which in turn raised considerable concerns about the increasingly serious environmental pollution caused by electronic waste. Herein, a radial inward directional freezing-drying method was proposed to prepare biobased, degradable and directional porous carboxymethyl chitosan/lignosulfonate sodium aerogel with dual-conductive network constructed by carboxylated multiwalled carbon nanotubes (C-CNTs) and MXene for piezoresistive pressure sensor. Owing to the synergistic conductive network of C-CNTs and MXene as well as directional porous structure, the sensor exhibited high sensitivity (2.33 kPa−1 in 0–10 kPa, 1.04 kPa−1 in 10–31 kPa, 0.53 kPa−1 in 31–53 kPa), fast response (response/recovery time of 140/60 ms), and excellent repeatability (2,000 loading–unloading cycles). Moreover, the sensor was successfully applied for human motion detection, healthy monitoring, micro-expression identification, and speech recognition. In addition, the aerogel for sensor was able to be completely degraded within 70 h in 1 M hydrochloric acid solution or partially degraded in boiling water within 2.5 h for the recycling of conductive materials, which was beneficial for not only environment protection but also saving resource. Our findings conceivably stand out as a new strategy to prepare environmentally friendly and high-performance piezoresistive pressure sensor and will promote the further development and application of flexible electronics.

Highly sensitive volatile organic compounds monitoring enabling by silver-nanowire@metal–organic frameworks core-shell heterostructure

Metal–organic frameworks (MOFs) hold great promise as advanced chemical sensing materials due to their high surface area and tunable surface chemistry. However, due to the inherent conductivity, building a highly sensitive MOFs-based gas sensor for real-time monitoring hazardous gas operated at room temperature (RT) is still a huge challenge. Herein, an in-situ anchoring strategy is proposed to construct a 1D-0D core-shell heterostructure by integrating silver nanowires (AgNWs) with highly conductivity and Zn-MOF with high specific surface area. The incorporation of AgNWs establishes a highly conductive network architecture to facilitate rapid charge transport while preventing the Zn-MOF nanoparticles from agglomeration, ensuring an effective transmission highway for target gas molecules. Meanwhile, the Zn-MOF nanoparticles induce remarkable absorption capacity and contribute high gas response. By strategically amalgamating the inherent distinctive virtues of the individual components and capitalizing on the synergistic benefits arising from the composite, the sensors hinged upon the refined AgNWs@Zn-MOF (A@Z) heterostructure unveiled remarkable response value of 27 to 20 ppm ethanol at RT, accompanied by a low detection limit of 1 ppm. Moreover, the A@Z sensor further showcases superior selectivity and repeatability. This work offers a fresh standpoint for the fabrication of MOF-based heterostructures, fostering advancements in diverse applications.

Morphotropic phase boundary evolution with synergistic effect of sintering temperature to improve electrocaloric and energy storage performances of lead-free Ba0.95Ca0.05Sn0.09Ti0.91O3 (BCST) ceramic

The designing of lead-free ferroelectric ceramics for application in environment-friendly efficient electrocaloric cooling and energy storage devices is still challenging and need to be considered. A major challenge, however, is how to simultaneously achieve higher polarization and low hysteresis loss at low applied electric field. In this perspective, we comprehensively investigated sol-gel derived Ba0.95Ca0.05Sn0.09Ti0.91O3 (BCST) ceramics by varying the sintering temperature to explore its electrocaloric and energy storage capabilities. The excellent ∆T ∼ 1.03 K, ∆S ∼ 1.32 J/kg.K and high electrocaloric responsivity ΔT/ΔE ∼ 0.34 K.mm/kV at very low applied electric field of 30 kV/cm are obtained for pristine BCST sample sintered at 1400 °C. Additionally, we observed Wrec ∼ 136.18 mJ/cm3 and giant energy conversion efficiency η ∼ 94.29 % under very low applied electric field of 30 kV/cm for the same BCST sample. Our results reveal that control of sintering temperature contributes to a favorable and stable microstructure, including R-O-T phase coexistence and substitutional heterogeneity-induced nano-polar regions (PNRs) which strengthens the Polarization and supports the thin hysteresis loop. Thus all these factors simultaneously contributing to make BCST ceramic as a potential material for applications in advanced electrocaloric cooling and energy storage capacitors.

NLRP3 Inflammasomes: Dual Function in Infectious Diseases.

The Nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasome has been the most distinctive polymer protein complex. After recognizing the endogenous and exogenous danger signals, NLRP3 can cause inflammation by pyroptosis and secretion of mature, bioactive forms of IL-1β and IL-18. The NLRP3 inflammasome is essential in the genesis and progression of infectious illnesses. Herein, we provide a comprehensive review of the NLRP3 inflammasome in infectious diseases, focusing on its two-sided effects. As an essential part of host defense with a protective impact, abnormal NLRP3 inflammasome activation, however, result in a systemic high inflammatory response, leading to subsequent damage. In addition, scientific evidence of small molecules, biologics, and phytochemicals acting on the NLRP3 inflammasome has been reviewed. We believe that the NLRP3 inflammasome helps us understand the pathological mechanism of different stages of infectious diseases and that inhibitors targeting the NLRP3 inflammasome will become a new and valuable research direction for the treatment of infectious diseases.

Efficacy and Safety of Immune Checkpoint Inhibitor Combination Therapy for Dysphagia in Patients with Advanced Esophageal Cancer.

Background/Objectives: Recently, pembrolizumab plus 5-fluorouracil and cisplatin (FP), nivolumab plus FP, and nivolumab plus ipilimumab have become the first-line treatments for patients with advanced esophageal cancer. However, the treatment efficacy in primary tumors has not been reported. We assessed the outcomes of these treatments in advanced esophageal cancer, specifically focusing on esophageal dysphagia improvements and the primary tumor response. Methods: This retrospective study was conducted between October 2021 and November 2023. We investigated 23 patients with esophageal cancer and dysphagia who received an immune checkpoint inhibitor (ICI) plus FP or nivolumab plus ipilimumab. Results: The median progression-free survival (PFS) was 10.6 months (95% confidence interval [CI]: 9.0-12.5), and the median overall survival was not reached (95%CI: 13.0-NA). Improvement in dysphagia was observed in 19/23 (82.6%) patients, with a median time to improvement of 26 days (range: 15-77 days) and a median dysphagia PFS of 12.6 months (range: 8.1-NA months). Ten patients experienced immune-related adverse events (irAEs): seven had interstitial pneumonia, and three had thyroid dysfunction, pituitary dysfunction, and rash, respectively. Conclusions: Although there was a high frequency of irAEs, ICI for esophageal cancer achieved high response rates and prolonged survival. The observed improvement in dysphagia suggests the potential efficacy of the treatment against primary tumors.

Baseline Gut Microbiota Was Associated with Long-Term Immune Response at One Year Following Three Doses of BNT162b2.

This study explored neutralizing IgG antibody levels against COVID-19 decline over time post-vaccination. We conducted this prospective cohort study to investigate the function of gut microbiota in the host immune response following three doses of BNT162b2. Subjects who received three doses of BNT162b2 were recruited from three centers in Hong Kong. Blood samples were obtained before the first dose and at the one-year timepoint for IgG ELISA to determine the level of neutralizing antibody (NAb). The primary outcome was a high immune response (NAb > 600 AU/mL). We performed shotgun DNA metagenomic sequencing on baseline fecal samples to identify bacterial species and metabolic pathways associated with high immune response using linear discriminant analysis effect size analysis. A total of 125 subjects were recruited (median age: 52 years [IQR: 46.2-59.0]; male: 43 [34.4%]), and 20 were regarded as low responders at the one-year timepoint. Streptococcus parasanguinis (log10LDA score = 2.38, p = 0.003; relative abundance of 2.97 × 10-5 vs. 0.03%, p = 0.001), Bacteroides stercoris (log10LDA score = 4.29, p = 0.024; relative abundance of 0.14% vs. 2.40%, p = 0.014) and Haemophilus parainfluenzae (log10LDA score = 2.15, p = 0.022; relative abundance of 0.01% vs. 0, p = 0.010) were enriched in low responders. Bifidobacterium pseudocatenulatum (log10LDA score = 2.99, p = 0.048; relative abundance of 0.09% vs. 0.36%, p = 0.049) and Clostridium leptum (log10LDA score = 2.38, p = 0.014; relative abundance of 1.2 × 10-5% vs. 0, p = 0.044) were enriched in high responders. S. parasanguinis was negatively correlated with the superpathway of pyrimidine ribonucleotides de novo biosynthesis (log10LDA score = 2.63), which contributes to inflammation and antibody production. H. parainfluenzae was positively correlated with pathways related to anti-inflammatory processes, including the superpathway of histidine, purine, and pyrimidine biosynthesis (log10LDA score = 2.14). Among three-dose BNT162b2 recipients, S. parasanguinis, B. stercoris and H. parainfluenzae were associated with poorer immunogenicity at one year, while B. pseudocatenulatum and C. leptum was associated with a better response.

Predicting network members from partial contact records on social media: A machine learning approach

Surveys conducted on social groups often generate incomplete information due to imperfect response rates. Drawing on Facebook data from a nationally representative sample of graduating college students in Taiwan, we examined the extent to which partial contact records predict which Facebook users belong to a specific class. We first used data from classes with low to middle response rates to train a model for classmate prediction. Based on data from classes with high or perfect response rates, we simulated data by using four different sampling methods with various response rates, and applied the trained model on simulated data to classmate prediction. With a minimal response rate of 40 percent, we achieved an accuracy rate of 90 percent and a true positive rate of 86 percent. Chronological order sampling had the best prediction performance, followed closely by popularity sampling, then by random sampling, and lastly by unpopularity sampling.

A nucleoside-modified mRNA vaccine forming rabies virus-like particle elicits strong cellular and humoral immune responses against rabies virus infection in mice

ABSTRACT Rabies is a lethal zoonotic disease that threatens human health. As the only viral surface protein, the rabies virus (RABV) glycoprotein (G) induces main neutralizing antibody (Nab) responses; however, Nab titre is closely correlated with the conformation of G. Virus-like particles (VLP) formed by the co-expression of RABV G and matrix protein (M) improve retention and antigen presentation, inducing broad, durable immune responses. RABV nucleoprotein (N) can elicit humoral and cellular immune responses. Hence, we developed a series of nucleoside-modified RABV mRNA vaccines encoding wild-type G, soluble trimeric RABV G formed by an artificial trimer motif (tG-MTQ), membrane-anchored prefusion-stabilized G (preG). Furthermore, we also developed RABV VLP mRNA vaccine co-expressing preG and M to generate VLPs, and VLP/N mRNA vaccine co-expressing preG, M, and N. The RABV mRNA vaccines induced higher humoral and cellular responses than inactivated rabies vaccine, and completely protected mice against intracerebral challenge. Additionally, the IgG and Nab titres in RABV preG, VLP and VLP/N mRNA groups were significantly higher than those in G and tG-MTQ groups. A single administration of VLP or VLP/N mRNA vaccines elicited protective Nab responses, the Nab titres were significantly higher than that in inactivated rabies vaccine group at day 7. Moreover, RABV VLP and VLP/N mRNA vaccines showed superior capacities to elicit potent germinal centre, long-lived plasma cell and memory B cell responses, which linked to high titre and durable Nab responses. In summary, our data demonstrated that RABV VLP and VLP/N mRNA vaccines could be promising candidates against rabies.

SPR humidity dynamic monitoring method via PVA sensing membrane thickness variation and image processing techniques

Humidity monitoring is paramount in diverse applications, industrial, and medical applications. Surface Plasmon Resonance (SPR) is an optical detection technique capable of sensing various environmental parameters through changes in reflected optical spectra and has garnered significant attention. Typically, SPR sensing employs a single-point detection strategy with the sample at a fixed concentration to achieve optimal sensitivity, limiting its application in dynamic environmental testing. This study proposes an image-based SPR humidity monitoring method, integrating SPR with image processing, enabling dynamic parameter reconstruction, and achieving high responsiveness. Au-PVA is used as a sensing film. To attain the best sensing film thickness, sensing film thicknesses ranging from 94.0 nm to 243.3 nm were tested. Through optimizing film thickness and image data processing, high precision and dynamic responsiveness were achieved. Experimental results demonstrate a response time of 84 ms and an average relative prediction error of 1.57 % for the sensor. Our research holds significant promise for dynamic and accurate humidity detection.

Soybean protein-reinforced highly extensible and self-adhesive hydrogels with ultra-sensitive strain sensing for flexible electronic devices

There is a growing interest in utilizing sustainable and renewable biomass resources to construct high-performance hydrogels for flexible electronics. In this study, soybean protein isolate (SPI) with abundant functional groups was introduced into the hydrogel matrix, while the connection between SPI, MXene and the polymer network was strengthened by hydrogen bonding. The sensible strategy overcame the problems of easy oxidation, agglomeration and weak interactions that existed in the binding of conductive filler MXene nanosheets with polymer chains. The prepared composite hydrogel exhibited integrated properties of high stretchability (>800 %), excellent self-adhesive property, plasticity, injectability, cyclic stability and durability in extreme environments (-20–60°C). Noteworthily, this hydrogel demonstrated high sensitivity and stable electrical resistance response to minor changes in tension, compression, bending, and temperature. In addition, this hydrogel exhibited excellent UV isolation and perspiration properties, making it ideal for practical applications of flexible devices in sports environments. The preparation of green, multipurpose, flexible electronics and human motion monitoring devices is made easier by the practical approach in this study.

Life style modification practices and associated factors among hypertensive patients at public hospitals in Ambo Town, Ethiopia, 2023

BackgroundModifiable lifestyle factors can increase or decrease the risk of hypertension. Despite emerging empirical evidence about efficacy of lifestyle modification in blood pressure control, little known and failed to appropriately counsel, hypertensive patients by health professionals on health lifestyle. General objectiveThe aim of this study is to assess magnitude of lifestyle modification practices and its associated factors among hypertensive patients. MethodsInstitution-based cross-sectional study was conducted among 344 hypertensive using systematic random sampling at public hospitals in ambo town from March 1–30, 2023. Data was collected using adapted structured questionnaire by interview. Logistic regression analysis model was fitted the data, no Multicollinearity detected among independent variables, Adjusted Odds Ratio at 95 % confidence interval was used, P-values less than or equal to 0.05 were considered statistically significant. Descriptive statistic (frequency, mean) used to explain the data. ResultsThe study has high response rate of 96.9 %. About 56.1 %, (95 % CI: [51.3–61.0]) had a good lifestyle modification practice. In this study, being employed (AOR=3.59, 95 % CI: [1.22–10.54]), Good self-efficacy (AOR=2.92, 95 % CI: [1.06–8.02]), Good knowledge (AOR=3.77 95 % CI: [1.81–7.85]), Normal body mass index (AOR=0.22, 95 % CI: [0.064–0.78]), being underweight (AOR=0.12, 95 % CI: [0.022–0.63]) were factors associated with life style modification practice. Conclusion and recommendationLife style modification practice is low in this study. Employment, knowledge, self-efficacy, and obesity were significantly associated. Recommendations include, promoting lifestyle modifications, and encouraging healthy behaviors.

MBE-grown ZnTe epitaxial layer based broadband photodetector with high response and excellent switching characteristics

Zinc telluride (ZnTe) epitaxial layers were grown on gallium arsenide (GaAs) (211) substrate at different growth temperatures by molecular beam epitaxy. The fabricated interdigitated metal semiconductor metal configuration-based photodetector (PD) on ZnTe epitaxial layers exhibited a stable and excellent photo response in a broad spectral range (250–550 nm) up to 125 °C. The room temperature and higher temperature (125 °C) values of maximum current, spectral responsivity and detectivity at an applied bias of 5 V and 550 nm wavelength were 3.5 × 10−8 A, 0.1 A W−1 and 1 × 1011 Jones and 1.7 × 10−6 A, 2.5 A W−1 and 1.5 × 1011 Jones, respectively. The maximum photo-to-dark-current ratio (PDCR) value at zero bias and 100 °C was obtained for the ZnTe layer grown at an optimum growth temperature of 380 °C. The high PDCR value exhibits the self-powered capability of the detector. Furthermore, the detector exhibits good on–off switching to the illuminating light with rise and decay times less than 0.29 s and 0.4 s, respectively, at room temperature. The dependence of the photo response on material quality was analysed by varying the substrate growth temperature. The broadband responsivity of the ZnTe-based PD shows its capability as a multicolour detector in the UV and visible region with the use of suitable blocking filters.

A magnetic carboxyl-functionalized covalent organic framework for the efficient enrichment of foodborne heterocyclic aromatic amines prior to UPLC-MS analysis

Foodborne heterocyclic aromatic amines (HAAs) are potent mutagens and carcinogens, posing significant health risks. Existing enrichment methods for HAAs need better adsorption selectivity and capacity for daily exposure assessment. This study hypothesized that introducing carboxylic groups into magnetic covalent organic frameworks (m-COFs) would improve HAAs adsorption by providing additional binding sites. Hence, we prepared a novel magnetic adsorbent, termed as Fe3O4@DOPA-TpPa-(COOH)2 capable of enhancing the HAAs detection through magnetic solid-phase extraction (MSPE) coupled with UPLC-MS. This sorbent demonstrated a large specific surface area (130.7 m2/g), high magnetic responsivity (21.05 emu/g), and robust stability, with an adsorption capacity (Qm[cal]: 81.82 mg/g) driven by electrostatic, LP − π/C-H − π interactions, and hydrogen bonding. Optimal MSPE conditions provided sensitive detection with a broad linear range (5–500 ng/mL), low limits of detection (0.01–7.01 ng/g), and excellent repeatability. Application to Cantonese mooncake samples showed satisfactory recoveries (62.12%–126.86%). This method offers a more accurate tool for detecting HAAs.

Updated efficacy of anti-TROP2 ADC ESG401 for first-line metastatic TNBC in phase 1b study.

2 Background: ESG401, a novel ADC comprising a humanized anti-TROP2 IgG1 monoclonal antibody conjugated to SN-38, with a Drug-Antibody Ratio of 8 and a stable cleavable linker, demonstrated preliminary efficacy and tolerability in the Phase Ia trial (Ma, ASCO 2023). This report presents updated results from the first-line mTNBC cohort of the Phase Ib trial and results from patients with brain metastases. Methods: Patients (pts) aged ≥18 years with confirmed local advanced/unresectable or metastatic TNBC, without prior metastatic treatment, received ESG401 (16 mg/kg IV on Day 1,8, and 15, with a 28-day cycle) until unacceptable toxicity, progressive disease, or consent withdrawal. Results: As of the cutoff date (Apr 7th, 2024), 24 pts in the cohort received ≥1 dose of ESG401. Median age was 53 years (range: 33-73), 33.3% were ECOG 0, 25.0% were de novo stage IV, 70.8% had visceral disease (8.3% brain, 33.3% liver, 50.0% lung). Updated efficacy results are shown in the Table. Thirteen pts (68.4% of 19 efficacy evaluable pts) remained on treatment, with the longest on-treatment duration being 10.3 mons. Median PFS has not been reached. Two pts in this cohort had brain metastases, with one achieving a complete intracranial response and an overall response of PR, and the other demonstrating overall PR with a -13.8% reduction in brain lesion size. Totally 16 pts with brain metastases were enrolled in the entire Phase Ia/1b trials, for these pts, the best overall intracranial response rate was 31%, with an intracranial disease control rate of 75%, while the corresponding overall response rate and disease control rate were 50% and 69%, respectively. The response of intracranial lesions to the treatment is consistent with that of extracranial lesions. The safety profile of ESG401 remained consistent with previous reports, showing no new or unexpected safety signals. Conclusions: ESG401 monotherapy demonstrates promising antitumor activity in 1L mTNBC pts, characterized by a high response rate and durable response. The ORR numerically exceeded that reported in the BEGONIA Study arm 7 in which pts with the same indication were treated with TROP-2 ADC and PD-L1 combination therapy. Additionally, ESG401 shows clear potential for treating HER2-negative breast cancer with brain metastases, including mTNBC and HR+/HER2- BC. These results support further clinical investigation of ESG401. Clinical trial information: NCT04892342 . [Table: see text]

Prospective Assessment of Serum Lipid Alterations in Chronic Hepatitis C Patients Treated with Direct Acting Antivirals: Insights Six Months Post Sustained Virological Response.

Background and Objectives: Chronic hepatitis C virus (HCV) infection is intricately linked with dysregulation of lipid metabolism. In particular, cholesterol plays a crucial role in HCV replication. Direct-acting antiviral agents (DAAs) therapy has revolutionized the hepatitis C treatment landscape, achieving high rates of sustained virological response (SVR). However, viral clearance comes with some alterations in lipid-related markers. This prospective study aimed to evaluate the impact of HCV clearance on lipid homeostasis and non-invasive liver fibrosis markers in hepatitis C patients treated with DAAs. Material and Methods: Fifty-two patients with varying degrees of fibrosis treated with DAAs therapy were evaluated at baseline and 24 weeks post-SVR. Lipid profiles and non-invasive liver fibrosis markers were assessed. Results: Our findings revealed an increase in total cholesterol, triglyceride, and LDLc (low-density lipoprotein cholesterol) levels at 24 weeks post-SVR, alongside an improvement in serum liver enzymes. Although improvements in liver stiffness were observed in non-invasive tests, there was an increase in lipid-related markers post-SVR. Conclusions: This suggests a potential increased cardiovascular risk despite improvements in liver function and fibrosis, highlighting the necessity for statin therapy in some cases and extended follow-ups for these patients. These findings underscore the importance of closely monitoring lipid profiles in chronic hepatitis C patients post-SVR, as well as the potential need for statin therapy to mitigate cardiovascular risk. Additionally, extended follow-up is essential to assess long-term outcomes and ensure the optimal management of these patients.