The microstructures and fatigue properties of the TNTZ added with Y or Y2O3 have been investigated. The results indicate that TNTZ added with Y or Y2O3 are all found to be composed of β phase and the small amount of Y2O3. The grain size of TNTZ added with Y or Y2O3 is smaller than that of TNTZ. The Young’s modulus of TNTZ added with Y or Y2O3 are maintained at a low level, and Young’s modulus of TNTZ added with Y is smaller than that of TNTZ added with Y2O3. The mechanical properties are both improved by adding Y or Y2O3, while the tensile strength of TNTZ added with Y2O3 is slightly higher than that added with Y. The high cycle fatigue limit of the alloys added with Y or Y2O3 are similar, while the low cycle fatigue strength of TNTZ added with Y is higher than that added with Y2O3. The improvement in fatigue properties ascribes to the microstructure refinement and the pining effect of Y2O3 particles. On the other hand, Y elements form Y2O3 with the Oxygen elements in the matrix, thus lead to the weakening of the Oxygen solution effect.