This study investigates the intricate interplay between higher education and environmental pollution in the MENA region from 2000 to 2018. Employing a comprehensive analytical approach, including cross-dependency tests alongside panel unit root tests, Kao cointegration tests, fixed effect GLS, DOLS (dynamic ordinary least squares), FMOLS (fully modified ordinary least squares) estimations, and Granger causality tests, the research focuses on three critical control variables—Unemployment, gross domestic product, and the globalization index. GLS analysis reveals a positive correlation between higher education, globalization, GDP per capita, and CO2 emissions in the MENA region. However, delving deeper using DOLS and FMOLS, a nuanced perspective emerges, indicating the role of higher education and globalization in mitigating CO2 emissions in the long run, challenging the Kuznets curve hypothesis. Additionally, causality tests highlight the significant influence of higher education and globalization on CO2 emissions. These findings address a critical gap in the understanding of environmental dynamics in the MENA region and offer valuable insights for policymakers and governments, informing targeted interventions and policies for sustainable development and reduced CO2 emissions.