In this study, the lubrication and cooling properties of eco-friendly graphite nanofluids in MQL grinding were investigated. Grinding forces, subsurface temperature of workpiece, surface roughness, micro-hardness and metallographic observations of ground surfaces were employed to evaluate the performance of synthesized nanofluids as lubricant under different grinding parameters. The results were also compared with grinding in dry, pure MQL and flood cooling conditions. The results showed that the tangential forces and force ratios in grinding using graphite nanofluid MQL are lower than that of other lubricating methods especially at extreme cutting parameters. Also, application of graphite nanofluid MQL reduced the grinding temperature at high velocities of workpiece. These reductions could be attributed to the formation of a tribofilm on the ground surface by the present of graphite nanoparticles in the wheel-workpiece interface. Additionally, the presence of this tribofilm in the contact area generated a smooth surface even at high depth of cut and velocity of workpiece. Furthermore, the micro-hardness of ground surfaces increased in graphite nanofluid MQL grinding because of infiltration of graphite nanoparticles in the grinding surface and the plastic deformation of subsurface of workpiece.