High Chlorogenic Acid Content Research Articles

Enzyme inhibitory and antioxidant activities and HPLC quantification of chlorogenic acid in Helichrysum stoechas (L.) Moench and H. stoechas subsp. barrelieri (Ten) Nyman

The inhibitory effects of ethanol (80%) and aqueous extracts of Helichrysum stoechas (L.) Moench and H. stoechas subsp. barrelieri (Ten) Nyman on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), two sister enzymes associated with pathogenesis of Alzheimer's disease (AD), as well as on elastase and collagenase, linked to inflammation and skin aging, were investigated. Simultaneously, antioxidant activity of the extracts was assessed through DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), and metal-chelating activity assays, since oxidative damage plays a critical role in both AD pathophysiology and skin aging. Total phenol and flavonoid contents in the extracts were spectrophotometrically determined. The highest AChE inhibitory activity (44.60 ± 4.4% at 2000 µg/mL) was found in the ethanol extract of H. stoechas subsp. barrelieri collected from Hatay, and the uppermost BChE inhibitory activity at same concentration was found in the aqueous extract of H. stoechas subsp. barrelieri collected from Izmir (80.24 ± 2.63%, IC50: 38.52 ± 1.41 µg/mL). Both of them inhibited AChE and BChE in a concentration-dependent manner. Nevertheless, none of the extracts from the two plants inhibited the elastase and collagenase. Although both ethanolic and aqueous extracts had significant antioxidant activity in DPPH radical scavenging and FRAP assays, they demonstrated inadequate antioxidant activity in metal-chelating assay. Chlorogenic acid was quantified in the extracts using HPLC. The mentioned two extracts having strong cholinesterase (ChE) inhibition had also the highest chlorogenic acid content. The ethanol extract of H. stoechas (Hatay sample) and the aqueous extract of H. stoechas (Izmir sample) seem to contain promising ChE inhibitors, which deserve further investigation.

Technological and sensory properties of plant-based meat analogues containing fermented sunflower protein concentrate

Sunflower press cake is a protein-rich sidestream from oil production, that is potentially suitable for human consumption, but its utilisation is hindered by the high content of chlorogenic acid (CGA), an abundant anti-nutritional factor. Here we show that processing sunflower protein concentrate (SP) by fermentation (fSP) reduces the CGA content while increasing the level of free amino acids, but impairs the formation of fibrillar structures and generates a sour taste. After fermentation of SP pH was shifted to neutral (fSP7). Meat analogues based on SP, fSP, fSP7, and pea protein isolate were prepared by high-moisture extrusion processing, which reduced CGA levels further. The texture and colour of the extrudates were both improved by the neutral pH shift. Finally, the odour, flavour and structure were assessed by a trained sensory panel. We found that fermentation followed by a neutral pH shift encouraged the formation of fibrillar structures and prevented sour taste attributes in the meat analogue. Our data will facilitate the development of sunflower protein products that are more palatable and nutritionally beneficial for humans.

Comparison of different pretreatments on drying characteristics, physical properties, nutritional compounds and antioxidant activity of heat pump‐dried longan pulp

SummaryDrying pretreatments are usually considered as effective way to improve the quality of dried fruit products. The results of this research showed that the blanching (BL), ultrasonic (US) and freeze–thaw (FT) pretreatments could significantly shorten the drying time, and improve the rehydration capacity of the dried longan pulp. Compared with the control group (CK, without pretreatment), more irregular inner micropores were formed in the dried longan pulp treated with BL, US and FT. Besides, the brightness and yellowness of dried longan pulp with pretreatments were higher than those of the CK. The higher retention rates of total phenolic content (292.54 mg/100 g d.b.) and ascorbic acid (2.38 mg g−1 d.b.) were observed in the dried longan pulp treated with BL. Meanwhile, a higher chlorogenic acid content was found in the samples treated with FT (3.68 mg/100 g d.b.) and radio frequency (RF) (3.10 mg/100 g d.b.). Furthermore, the highest retention of a total free amino acid (1843.13 mg/100 g d.b) was exhibited by RF‐treated longan pulp. This work guides how pretreatments are applied prior to longan pulp drying.

Variation in Phenolic Compounds, Antioxidant and Antibacterial Activities of Extracts from Different Plant Organs of Meadowsweet (Filipendula ulmaria (L.) Maxim.).

Meadowsweet (Filipendula ulmaria (L.) Maxim.) has been widely used in the treatment of various diseases. The pharmacological properties of meadowsweet are derived from the presence of phenolic compounds of a diverse structure in sufficiently large quantities. The purpose of this study was to examine the vertical distribution of individual groups of phenolic compounds (total phenolics, flavonoids, hydroxycinnamic acids, catechins, proanthocyanidins, and tannins) and individual phenolic compounds in meadowsweet and to determine the antioxidant and antibacterial activity of extracts from various meadowsweet organs. It was found that the leaves, flowers, fruits, and roots of meadowsweet are characterized by a high total phenolics content (up to 65 mg g-1). A high content of flavonoids was determined in the upper leaves and flowers (117-167 mg g-1), with high contents of hydroxycinnamic acids in the upper leaves, flowers, and fruits (6.4-7.8 mg g-1); high contents of catechins and proanthocyanidins in the roots (45.1 and 3.4 mg g-1, respectively); and high tannin content in the fruits (38.3 mg g-1). Analysis of extracts by high-performance liquid chromatography (HPLC) showed that the qualitative and quantitative composition of individual phenolic compounds in various parts of the meadowsweet varied greatly. Among the flavonoids identified in meadowsweet, quercetin derivatives dominate, namely quercetin 3-O-rutinoside, quercetin 3-β-d-glucoside, and quercetin 4'-O-glucoside. Quercetin 4'-O-glucoside (spiraeoside) was found only in the flowers and fruits. Catechin was identified in the leaves and roots of meadowsweet. The distribution of phenolic acids across the plant was also uneven. In the upper leaves, a higher content of chlorogenic acid was determined, and in the lower leaves, a higher content of ellagic acid determined. In flowers and fruits, a higher contents of gallic, caftaric, ellagic, and salicylic acids were noted. Ellagic and salicylic acids were also dominant among phenolic acids in the roots. Based on the results of the analysis of antioxidant activity in terms of the ability to utilize the radicals of 2,2-diphenyl-1-picrylhydrazine (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazolino-6-sulfonic acid) (ABTS) and in terms of iron-reducing ability (FRAP), the upper leaves, flowers, and fruits of meadowsweet can be considered plant raw materials suitable to obtain extracts with high antioxidant activity. Extracts of plant fruits and flowers also showed high antibacterial activity against the bacteria Bacillus subtilis and Pseudomonas aeruginosa.

LncRNA-mediated ceRNA regulatory network provides new insight into chlorogenic acid synthesis in sweet potato.

Sweet potato (Ipomoea batatas L.) is considered a highly nutritional and economical crop due to its high contents of bioactive substances, such as anthocyanin and chlorogenic acid (CGA), especially in leaves and stems. The roles of noncoding RNAs (ncRNA), including long noncoding RNA (lncRNA) and microRNA (miRNA), in CGA synthesis, are still unknown. In this study, the differentially expressed (DE) mRNAs, miRNAs, and lncRNAs in two leafy vegetable genotypes "FS7-6-14-7" (high CGA content) and "FS7-6" (low CGA content) were identified. The cis-regulation between lncRNA and mRNA was analyzed. Then, the CGA synthesis-related modules MEBlue and MEYellow were identified to detect trans-regulation mRNA-lncRNA pairs. The GO and KEGG annotations suggested that mRNA in these two modules was significantly enriched in the secondary metabolite synthesis biosynthesis category. A competing endogenous RNAs (ceRNA) network, including 8730 miRNA-mRNA and 444 miRNA-lncRNA pairs, was constructed by DEmiRNA target prediction. Then, a CGA synthesis-related ceRNA network was obtained with lncRNA and mRNA from MEBlue and MEYellow. Finally, one relational pair, MSTRG.47662.1/mes-miR398/itb04g00990, was selected for functional validation. Overexpression of lncRNA MSTRG.47662.1 and mRNA itb04g00990 increased CGA content in both tobacco and sweet potato callus, while overexpression of miRNA mes-miR398 decreased CGA content. Meanwhile, regression analysis of the expression patterns demonstrated that MSTRG.47662.1, acting as a ceRNA, promoted itb04g00990 expression by competitively binding mes-miR398 in CGA synthesis in sweet potato. Our results provide insights into how ncRNA-mediated ceRNA regulatory networks likely contribute to CGA synthesis in leafy sweet potato.

Influence of year, genotype and cultivation system on nutritional values and bioactive compounds in tomato (Solanum lycopersicum L.)

Two tomato genotypes were grown in open field by three cultivation systems (one conventional and two distinct organic for mulching) in three years, 2015, 2016 and 2017. Yields, sugars, organic acids, amino acids, ascorbic acid, biothiols, carotenoids and phenols were measured.Weather conditions largely differed among harvest years, with summer 2016 rainier and less warm, and an opposite summer 2017.Organic systems had lower yields than conventional one but also, interestingly, lower waste percentages. Furthermore, tilled and no-tilled organic systems provided comparable yields.With respect to 3-year average, sugars were higher in 2017, acids in 2016 and in organic fruits, and amino acids increased in 2015 and in conventional samples.A higher glutathione content was found in organic samples, and higher carotenoids in 2017. Phenols increased in 2016, with a higher chlorogenic acid content in organic tomatoes.Some differences between genotypes were observed, highlighting their different adaptability to growing systems.

Topical gel containing phenolic-rich extract from Ipomoea pes-capre leaf (Convolvulaceae) has anti-inflammatory, wound healing, and antiophidic properties

The growing use of phytotherapy in clinical practice arouses interest in studies using medicinal plants as active ingredients for new medicines. Ipomoea pes-caprae has a wide medicinal use in the treatment of inflammatory disorders, skin wounds, stings, and painful rheumatic processes. Assayed in this study are the physicochemical characterization of a gel developed with this extract and the evaluation of its anti-inflammatory and healing efficacy, in addition to its antiedematogenic action on Bothrops snake envenoming in mice. The qualitative and quantitative analyses of the hydroethanolic extract by mass spectrometry showed 18 phenolic compounds, highlighting a high content of chlorogenic acid (0.92 µg/g), neochlorogenic acid (6.07 µg/g), and isochlorogenic acid (0.80 µg/g) compounds. The formulation was stable in relation to the physical-chemical characteristics during the time of analysis and was considered safe for topical treatment in animals, causing no skin irritation. Although the results have shown an absence of activity in the model of ear edema induced by croton oil (acute inflammation), the herbal gel efficiently inhibited carrageenan paw edema and chronic ear edema induced by multiple applications of croton oil, which may indicate the possible performance under the kinin pathway such as bradykinin, histamine, and serotonin. Wound healing in the group treated with the I. pes-caprae gel was accelerated compared with the placebo group, also confirmed through histological data. Edema induced by Bothrops erythromelas snake venom was efficiently reduced in the treatment with I. pes-caprae gel associated with the antibothropic-crotalic serum, whereas the antivenom alone was not effective. This approach presents a promising formulation based on I. pes-caprae with potential therapeutic use for inflammatory disorders.

Phytochemical Profile, Antioxidant and Wound Healing Potential of Three Artemisia Species: In Vitro and In Ovo Evaluation

Skin injuries, and especially wounds of chronic nature, can cause a major negative impact on the quality of life. New efficient alternatives are needed for wound healing therapy and herbal products are being investigated due to a high content of natural compounds with promising healing activity. For this purpose, we investigated three Artemisia species, Artemisia absinthium L. (AAb), Artemisia dracunculus L. (ADr) and Artemisia annua L. (AAn). Ethanolic extracts, containing different polyphenolic compounds, elicited strong antioxidant activities in the DPPH assay, comparable to ascorbic acid. Human ketratinocyte proliferation was stimulated and wound closure was enhanced by all three extracts at concentrations of 100 µg/mL. The Artemisia extracts modulated angiogenesis by increasing vessel formation, especially following treatment with A. annua and A. dracunculus, extracts with a significantly higher content of chlorogenic acid. Good tolerability and anti-irritative effects were also registered in ovo, on the chorioallantoic membrane (CAM). The three Artemisia species represent promising low-cost, polyphenol-rich, antioxidant, safe alternatives for wound care treatment.

Comparative analysis of apocarotenoids and phenolic constituents of Crocus sativus stigmas from 11 countries: Ecological impact.

The chemical compositions of 15 saffron samples from 11 countries (Morocco, India, Italy, Spain, Germany, Switzerland, Iran, Lithuania, Ukraine, Australia, and Azerbaijan) were evaluated. The samples were analyzed regarding the impact of environmental factors on the composition of apocarotenoids and phenolic constituents. Quantification of saffron metabolites was carried out using high-performance liquid chromatography. It was found that the high content of chlorogenic acid (0.2 mg/g, Ukraine) and ferulic acid (0.28 mg/g, India) was controlled by the duration of solar radiation during plant development. The accumulation of caffeic acid (the higher content 4.88 mg/g, Ukraine) in stigmas depended on the average air temperature. In contrast, the total crocins content according to the correlation analysis depended on the duration of solar radiation, the solar UV index, and the soil type. Rutin was found in all samples (0.83-8.74 mg/g). The highest amount of crocins (average 382.45 mg/g) accumulated in saffron from Italy and Ukraine. Crocins, picrocrocin, safranal, and rutin can further serve as saffron quality markers. All validation parameters were satisfactory and high-performance liquid chromatography methods could be successfully applied for the composition assessment of saffron metabolites. Saffron extracts showed the highest antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli (MICs 62.5-125 µg/ml).

Evaluasi pasca panen, cacat mutu dan atribut kimia (kafein, asam klorogenat) kopi robusta Lampung Barat (studi kasus gapoktan di Lampung Barat)[Identification of post-harvest, quality defects, and chemical characteristics (caffeine, chlorogenic acids) of West Lampung robusta coffee beans (Case Study of Gapoktan in West Lampung

Post-harvest Robusta coffee conducted by local farmers in West Lampung consisted of two post-harvest processes: dry processing (natural, honey) and wet processing (full wash). The study aimed to identify the post-harvest process stages, the value of quality defects and to characterize the chemical composition (chlorogenic acid, caffeine) of coffee beans from different post-harvest processes produced by Gapoktan Triguna Tribudi Syukur, West Lampung. The research used survey and interview methods equipped with laboratory analysis. The research sample was determined by purposive sampling, a coffee producer (Gapoktan Triguna) who have been fostering coffee in West Lampung for 35 years. Coffee quality analysis refered to SNI 01-2907-2020. The results showed that the post-harvest process carried out by Gapoktan Triguna on honey, natural and full wash processing produced premium quality of of coffee beans (1-2 quality) with the highest number of quality defects produced were broken beans. The roasting process with semi-mechanical and mechanical machines with natural processing produced high chlorogenic acid content (>3%) and the caffeine content according to the SNI caffeine content (2%). Keywords: caffeine, chlorogenic acid, defects, post-harvest, Robusta

Sustainable Mulberry (Morus nigra L., Morus alba L. and Morus rubra L.) Production in Eastern Turkey

In this study, a total of 55 wild-grown mulberry landraces belonging to Morus alba L., Morus rubra L., and Morus nigra L. species (Rosales order, Moraceae family, Morus L. genus) were sampled around the Van Lake basin, and some fruit characteristics were determined. All landraces are naturally grown in the Lake Van basin under pesticide-free conditions. As fruit character, phenolic compounds (gallic acid, catechin, quercetin, protocatechuic acid, vanillic acid, rutin, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, and phlorizin) and organic acids (malic acid, citric acid, tartaric acid, succinic acid, fumaric acid and ascorbic acid) were determined. Principal component analysis (PCA) was performed to determine the correlation between mulberry species in terms of biochemical compounds. As a result of PCA-biplot analysis, two variations were sufficient to explain the correlation between phenolic compounds and organic acids. This ratio reveals that mulberry species are separated with sharp boundaries in terms of biochemical compounds. Chlorogenic acid and rutin content were high in all mulberry landraces. The highest chlorogenic acid content was detected in landrace 65VN03 belonging to M. rubra (3.778 mg/g), 65GV12 belonging to M. nigra (3.526 mg/g), 13AD08 belonging to M. rubra (2.461 mg/g), and 13AH02 belonging to M. rubra (6.246 mg/g) landraces. In terms of organic acid content, malic acid was the dominant organic acid for genotypes. The rich bioactive compounds make M. alba, M. rubra, and M. nigra landraces as cultivar candidates for breeding purposes. It is a valuable source of bioactive agents that may have prevented humans from oxidative-stress-related diseases.

Influence of light quality on growth and secondary metabolite in tissue cultures of Gardenia jasmonides, Variegata

The influence of white, blue and red Light-Emitting Diodes (LED) on the growth and the secondary metabolites production in calli and shoots cultures of Gardenia jasmonides, Variegata was evaluated. The white light generally has a powerful effect on both fresh and dry weights of the two types of cultures. White light treatment recorded (11.88 and 0.63 g) for the fresh and dry weight (in case of calli) followed by the blue light which recorded (11.78 and 0.60 g ), respectively. The extracts of shoots exposed to white LED light recorded the highest reducing efficiency (74.40 %). It could be justified as the shoots that exposed to the white light recorded the highest cholorogenic acid (CGA) compared to all the other treatments. Among different phenolic and flavonoids compounds which were tested in the calli and shoots cultures exposed to the different LED light quality, only the chlorogenic acid was detected in the different treatments in both cultures with varying quantities. The white LED light recorded the highest content of chlorogenic acid with both cultures, since it scored 52.43 µg / gdw with calli cultures versus 8783.53 µg / gdw with shoots cultures.

Resistance of genotypes and defence compounds against bacterial halo blight of coffee

AbstractBacterial halo blight of coffee (BHB), caused by Pseudomonas syringae pv. garcae, is one of the main coffee diseases and is economically important mainly in regions of higher altitudes and mild temperatures. The use of resistant cultivars in the management of diseases is the most efficient and least costly technology for producers. The Coffee Germplasm Bank of Minas Gerais State has genotypes with great genetic variability, some of which are sources of resistance to several diseases and pests. Thus, the objective of this study was to evaluate the potential of 34 genotypes from the Coffee Germplasm Bank of Minas Gerais State and five cultivars with resistance to BHB. We evaluated the expression of three plant defence genes (phenylalanine ammonia liase‐PAL, peroxidase‐POX, and cinnamyl alcohol dehydrogenase‐CAD) and quantified the content of total soluble phenolic compounds, lignin, caffeine, trigonelline, and chlorogenic acid in coffee leaves showing different levels of resistance to BHB. The cultivar IPR 102 (resistant control to BHB), the Catimor UFV 7158, Caturra Vermelho × CIFC H 79/1 UFV 339‐34, Catiguá MG2, Volutifolia Vermelho, Caturra Vermelho, Híbrido de Timor UFV 432‐02, Caturra Vermelho × CIFC H 288/14 UFV 319‐04, and Caturra Vermelho × CIFC H 79/1 UFV 339‐02 genotypes showed low disease severity. The UFV 7158 genotype showed higher PAL and POX expression levels than Mundo Novo 376/4 cultivar. The IPR 102 cultivar and UFV 7158 genotype showed high chlorogenic acid content and low caffeine and trigonelline contents.

Shifting of Physicochemical and Biological Characteristics of Coffee Roasting Under Ultrasound-Assisted Extraction.

Ultrasound-assisted extraction (UAE) is an effective tool for the extraction of natural antioxidants. Thus, differentially roasted Arabica-coffee beans known as light (LC), medium (MC), and dark coffee (DC) were prepared and extracted under the influence of UAE. Following that, they were examined specifically on theirs physicochemical and biological characteristics: nutritional values, pH, °Brix, antioxidant activities, polyphenol content, caffeine, and chlorogenic-acid levels. Various parameters, such as extraction temperatures (20, 40, and 80°C) and extraction time periods (5, 10, and 20 min), were examined. DC extract was less acidic than those on MC and LC extracts. LC showed higher moisture content than the MC and DC (1.56, 1.3, and 0.92%, respectively). MC displayed the highest polyphenol content and potent antioxidant activity. Caffeine and chlorogenic acid contents trend to decrease during roasting. The maximum caffeine level was found in MC at 80°C for 5 min (27.65 mg/g extract). The highest chlorogenic acid content was in LC at 80°C for 10 min (16.67 mg/g extract). The caffeine and chlorogenic acid contents were related to the polyphenol content and depended on the roasting and extraction conditions. These results suggest that the UAE at various temperature and extraction time period may alter the physicochemical and biological characteristics of different coffee roasts.

Assessment of the Efficacy of Olive Leaf (Olea europaea L.) Extracts in the Treatment of Colorectal Cancer and Prostate Cancer Using In Vitro Cell Models.

Cancer is one of the most serious public health issues worldwide, ranking second only to cardiovascular diseases as a cause of death. Numerous plant extracts have extraordinary health benefits and have been used for centuries to treat a variety of ailments with few side effects. Olive leaves have a long history of medicinal and therapeutic use. In this study, the anti-cancer properties of an olive leaf extract were investigated in vitro using colorectal and prostate cancer cell lines (HT29 and PC3, respectively). A high-performance liquid chromatography analysis showed that the olive leaf extract contained a high chlorogenic acid content. Accordingly, chlorogenic acid may be related to the observed effects of the aqueous extract on cancer cells, including increased inhibition of cancer cell growth, migration, DNA fragmentation, cell cycle arrest at the S phase, reactive oxygen species (ROS) production, and altered gene expression. The effects of the extracts were greater in HT29 than in PC3 cells. These results suggest that chlorogenic acid, the main constituent in the olive extract, is a promising new anti-cancer agent. Further analyses should focus on its in vivo effects on colorectal tumor models, both alone and in combination with established agents.

Optimization and modeling of the chlorogenic acid extraction from a residue of yerba mate processing

Chlorogenic acid (CGA) and its hydrolysis products, quinic and caffeic acids are considered as fine chemicals and have a high commercial value. Yerba mate stems, useless residue from yerba mate processing, contains significant amounts of CGA. The goal of this study was to determine the best conditions for maximizing the CGA aqueous extraction from residues of yerba mate processing and to fit the extraction kinetics to empirical models. The effect of single factors such as solid-liquid ratio, numbers of extraction, temperature and time were studied for two particle sizes, using one factor at a time method and Response Surface Methodology (RSM).The highest and almost instantaneous CGA extraction (1.051 ± 0.015 gCGA L−1) was obtained using Ø particle size < 500 μm, at 65 °C, solid-liquid ratio of 1:20 and double stage extraction. For the largest particle size (2.5 × 5 mm > particle size >1 × 5 mm) the optimized conditions were 85 ± 5 °C and 25 ± 5 min, solid-liquid ratio of 1:20 and double stage extraction. For these last particles, the applied models of Pilosof et al. and Spiro and Jago showed a good agreement with the experimental and model calculated data.In the present study, it was possible to optimize the CGA extraction conditions for two particle sizes of yerba mate stems, in order to obtain extracts with high content of CGA that can be used in the production of others fine chemicals of interest in pharmaceutical industries.

Use of Sunflower Protein in Snack Bars

The production of new healthy low-carbohydrate, high-protein plant-based snacks is one of the food industry’s major trends. Alternative plant protein source, such as sunflower protein isolate, appears to be a promising ingredient for protein bars. Sunflower protein isolate is a secondary product with a high added value obtained from sunflower meal after the extraction of sunflower oil from seeds. One of the advantages of the sunflower protein compared to other plant proteins (such as soy, pea, and wheat) is the high content of helianthinin, which makes it highly digestible (up to 95%) and low allergenic, thus, helping avoid bloating, heartburn and upset stomach. It also has a high content of chlorogenic acid, making it an excellent antioxidant. This work aimed to determine the effect on nutritional, physicochemical, and organoleptic properties of snack bars if enriched with sunflower protein powder. Crude protein, fat, fiber, and moisture were assessed. Snack bars containing sunflower protein had low-moisture, were low in lipids, free of antinutrients, and were a good source of proteins. The results demonstrated that using isolate and concentrate, obtained from sunflower meal, in snack bars is a practical alternative to other plant proteins, which are often allergens. Not only does it help maintain the physicochemical characteristics of the product, but it helps improve the nutritional value as well.

REVIEW: MANFAAT ASAM KLOROGENAT DARI BIJI KOPI (Coffea) SEBAGAI BAHAN BAKU KOSMETIK

Chlorogenic acid(Chlorogenicacid,CGA) is one of the secondary metabolites produced from coffee beans. Chlorogenic acid is widely used in cosmetic preparations as an exfoliant (exfoliate). Apart from exfoliating, chlorogenic acid can also be used as an antioxidant, natural cosmetic coloring, antibacterial (acne), cellulite and slimming. In review this, first look at the comparison of the concentration of chlorogenic acid in the two types of coffee, namely Arabica coffee and Robusta coffee. Next, determine several cosmetic preparations that use coffee beans as a natural ingredient which contains chlorogenic acid. The purpose of review thisis to determine the comparison of the concentration of chlorogenic acid from Arabica coffee beans and robusta coffee which can be used in various cosmetic preparations. Based on the data obtained, the highest chlorogenic acid content was found in robusta green coffee beans with a concentration of 6.1-11.3 (g / 100g), while in Arabica green coffee beans with a concentration of 4.1-7.9 (g / 100g). So it can be concluded that the chlorogenic acid content in robusta coffee has a higher content compared to arabica coffee so that robusta coffee is more recommended to be used as a cosmetic raw material.

Phenolic profiles and antioxidant activities of exocarp, endocarp, and hypanthium of three pear cultivars grown in China

The phenolic composition of the exocarp, hypanthium, and endocarp from three pear cultivars: Jingbai, Korla and Crystal were measured by high-performance liquid chromatography method with diode array detection (HPLC-DAD), while antioxidant activities were investigated using DPPH radical andABTS radical cation scavenging activity, and FRAP. Ferulic acid, chlorogenic acid, and caffeic acid were detected as major components. The exocarp of the Jingbai pear had a relatively high chlorogenic acid content (0.691 mg kg-1) while the hypanthium and endocarp had the high chlorogenic acid content of the three pear varieties. The exocarp of the Korla pear had the highest ferulic acid and rutin content. The exocarp, hypanthium, and endocarp of the Crystal pear had the highest content of chlorogenic acid of the three pear varieties. The Crystal pear presented the highest total phenolic and flavonoid contents and had the highest antioxidant activities in terms of FRAP. The Jingbai pear exocarp presented the highest ABTS value, 83.62 ± 0.08%. The endocarp of the Crystal pear had the highest DPPH value, 87.30 ± 0.03%. Positive correlations were detected among chlorogenic acid, TPC, TFC, and FRAP. The results reveal that the different parts of Pyrus communis L. have effective antioxidant activity for the pharmaceutical application.

Dynamics of Phloridzin and Related Compounds in Four Cultivars of Apple Trees during the Vegetation Period

Apple trees (Malus domestica Borgh) are a rich source of dihydrochalcones, phenolic acids and flavonoids. Considering the increasing demand for these phytochemicals with health-benefitting properties, the objective of this study was to evaluate the profile of the main bioactive compounds—phloridzin, phloretin, chlorogenic acid and rutin—in apple tree bark, leaves, flower buds and twigs. The variety in the phenolic profiles of four apple tree cultivars was monitored during the vegetation period from March to September using chromatography analysis. Phloridzin, the major glycoside of interest, reached the highest values in the bark of all the tested cultivars in May (up to 91.7 ± 4.4 mg g−1 of the dried weight (DW), cv. ‘Opal’). In the leaves, the highest levels of phloridzin were found in cv. ‘Opal’ in May (82.5 ± 22.0 mg g−1 of DW); in twigs, the highest levels were found in cv. ‘Rozela’ in September (52.4 ± 12.1 mg g−1 of DW). In the flower buds, the content of phloridzin was similar to that in the twigs. Aglycone phloretin was found only in the leaves in relatively low concentrations (max. value 2.8 ± 1.4 mg g−1 of DW). The highest values of rutin were found in the leaves of all the tested cultivars (10.5 ± 2.9 mg g−1 of DW, cv. ‘Opal’ in September); the concentrations in the bark and twigs were much lower. The highest content of chlorogenic acid was found in flower buds (3.3 ± 1.0 mg g−1 of DW, cv. ‘Rozela’). Whole apple fruits harvested in September were rich in chlorogenic acid and phloridzin. The statistical evaluation by Scheffe’s test confirmed the significant difference of cv. ‘Rozela’ from the other tested cultivars. In conclusion, apple tree bark, twigs, and leaves were found to be important renewable resources of bioactive phenolics, especially phloridzin and rutin. The simple availability of waste plant material can therefore be used as a rich source of phenolic compounds for cosmetics, nutraceuticals, and food supplement preparation.