In this study, the effects of piston shapes on in-cylinder flow characteristics and mixture formation in the lean condition was analyzed using the PIV method and CFD (CONVERGE v2.4). The four-piston geometries were concave, flat, convex, and base pistons, and the pistons were mounted on a GDI engine with transparent quartz. To quantify in-cylinder flow characteristics, the mean velocity, tumble ratio, turbulent kinetic energy, and tumble center were calculated. In addition, to analyze the effects of the difference in piston shapes in detail, the velocity fraction was calculated by dividing the cylinder area into three. In-cylinder flow was compared for the case without injection and the case with injection. There were four timed injections at C.A. 420°, 480°, 540°, and 600°, and the mixture characteristics were investigated in the cases of 480° and 600° injection. In the intake process and early compression process, there was no significant difference in the quantitative values for the shape of the piston regardless of the presence or absence of injection. However, the effects of the piston crown designs increased in the later compression process. As a result, the highest turbulent kinetic energy was found using a flat piston with a relatively high tumble ratio and swirl ratio in the absence of injection. The mixture formation was similar for all piston crown designs when injected at the crank angle of 480°. However, when the crank angle of 600° was injected, a stagnant fuel area was formed using the base-type piston, so it was difficult to secure sufficient fuel near the center of the cylinder.