A ceiling cooling system integrated with a mechanical ventilation system has been widely used in modern buildings with large sensible cooling loads due to the high thermal comfort level and large energy efficiency. However, there is a lack of systematic research on the influence factors such as ceiling surface temperature and cooling load on the indoor air distribution and thermal environment, and the impact of ventilation system type in the ceiling cooling room is still unclear. Therefore, this paper presented an experimental study of indoor air distribution and thermal environment in a ceiling cooling (CC) room with mixing ventilation (MV), underfloor air distribution (UFAD) and stratum ventilation (SV); the ceiling surface temperature was 17 °C–26 °C and the internal or external cooling load was 41.5 W/m2–69.5 W/m2. The results showed that the vertical air temperature difference and contaminant removal effectiveness were 0.2 °C–0.4 °C and 0.53–0.85 with CC + MV, 0 °C–1.2 °C and 0.68–1.25 with CC + UFAD and 0.3 °C–0.9 °C and 0.50–0.83 with CC + SV, and the corresponding heat removal effectiveness and air diffusion performance index were 0.96–1.11 and 96–100%, 0.9–1.5 and 57–100% and 1.11–1.34 and 71–100%, respectively. Moreover, the difference between mean radiant temperature and air temperature and the predicted mean vote of thermal sensation were from 0 °C to 0.9 °C and between 0 and 0.26 with CC + MV, from −0.1 °C to 2.2 °C and between −0.1 and 0.42 with CC + UFAD and from −0.1 °C to 0.9 °C and between −0.2 and 0.13 with CC + SV. Hence, the ventilation system type clearly affected the indoor air distribution and thermal environment in the ceiling cooling room, and the experimental results would be beneficial for the design and control of a ceiling cooling system combined with a mechanical ventilation system in practice.