High Swelling Properties Research Articles

Preparation and Characterization of Chitosan-Wrapped Silver Nanoparticles Incorporated Poly (Vinyl Alcohol) Membranes for Purification of Biogas

A solution technique was adopted to develop membranes in which chitosan-wrapped silver nanoparticles (CS-wrapped Ag nanoparticles) were incorporated in poly(vinyl alcohol) (PVA). The morphological studies and the chemical compositions of the prepared membranes were investigated using the characterization techniques like Infrared Spectroscopy, Differential scanning colorimetry, Wide Angle X-ray Diffraction, Thermogravimetric analysis and scanning electron microscopy. The effects of CS-wrapped Ag nanoparticles on membrane swelling were systematically studied to know the gas separation performance of the membranes. TGA showed increased thermal stability of Ag-nanoparticles incorporated PVA membranes nearly twice that of plane PVA membranes. Further, SEM revealed that CS-wrapped Ag nanoparticles incorporation into the PVA membrane matrix showed good compatibility and high swelling properties.

Discussion of parameters used to distinguish suitable from less suitable HLRW bentonites

Abstract. Bentonites will be used in the construction of some high-level radioactive waste (HLRW) repositories mostly in combination with crystalline host rocks. They will be used both as a geotechnical barrier (compacted bentonite blocks) around the canisters and for backfilling. The bentonite should be stable in contact with cement pore water, minimize metal corrosion, be stable against erosion and various salt solutions, retard radionuclides, prevent canister displacement, possess high thermal conductivity, be stable against radioactive radiation, keep its swelling capacity even when dried, and, most importantly, should have a low hydraulic conductivity. Bentonites are natural materials (clays) which are dominated by swelling clay minerals called smectites. All bentonites, therefore, possess high water uptake capacity, swelling, and cation exchange properties. Different bentonites from different deposits worldwide differ with respect to their chemical and mineralogical composition, composition and charge distribution of the smectites, particle size and morphology, microstructure (arrangement of particles relative to each other), and interlayer population. All these parameters affect the performances of bentonites in different applications. The bentonite industry, therefore, compares different bentonites based on empirical investigations to produce superior products. Specifications which could be used to select a suitable HLRW bentonite were discussed by Kaufhold and Dohrmann (2016). Additional information has been published later (Kaufhold et al., 2020a, b). First of all, some of the above listed desired bentonite properties depend more on the degree to which it is compacted compared to the natural variability. High compaction decreases the hydraulic conductivity and increases thermal conductivity. In order to prevent canister displacement only a small swelling pressure is needed which is easily achieved by compaction with all bentonites. Generally, the type of exchangeable cation is the most important parameter determining bentonite properties such as swelling and rheology. Large scale deposition tests, however, proved that the cation population will readily equilibrate with the surrounding water. The initial type of exchangeable cation is, therefore, less relevant. More important is the Fe content which negatively affects the thermal and chemical stability. Structural Fe of the smectites can be reduced or oxidized by bacteria and radiation. The Fe content of the bentonite should therefore be low. Highly charged smectites proved to be less corrosive in combination with iron canisters because they provide more reducing conditions compared to low charged ones. Bentonites containing highly charged smectites should be preferred if Fe canisters are used. In the case of Cu canisters no effect of the charge could be found. Also, soluble or at least partly soluble components such as sulphates, sulphides, carbonates, and organic matter should be absent since their possible dissolution would decrease the dry density and hence the swelling pressure. The presence of reactive silica in some bentonites proved to buffer the dissolution reactions at the cement bentonite interface and hence could have a beneficial effect.

Making Dense Covalent Arabinoxylan Gels with High Swelling Properties: A Strategy Based on Water Extraction through Osmotic Compression

Making Dense Covalent Arabinoxylan Gels with High Swelling Properties: A Strategy Based on Water Extraction through Osmotic Compression

White Cabbage (Brassica oleracea L.) waste, as biowaste for the preparation of novel superabsorbent polymer gel

In order to reduce the pollution of the bio-waste cabbage to the environment and achieve recycling of biological waste resources, synthesis of a novel superabsorbent polymer gel based on waste cabbage (CB) using two monomers AMPS (2-acrylamide-2-methyl-1-propane sulfonic acid) and AA (acrylic acid). And the synthesis conditions were optimized, such as the ratio of AMPS and AA, initiator content, and crosslinker content. And the water retention, salt resistance and repeated swelling performance were investigated too. The chemical structure thermal stability and microscopic appearance of the superabsorbent polymer gel were investigated by Fourier transform infrared spectroscopy (FTIR), Thermal gravimetric analysis (TGA) and Scanning electron microscope (SEM). The results showed that the novel superabsorbent polymer gel exhibits excellent water absorbency, which can absorb ultrapure water, distilled water, tap water and 0.9% NaCl solution are 1914 g/g, 1726 g/g, 306 g/g and 114 g/g, respectively. This is higher than in related studies 30%− 40% in distilled water, 60%− 70% in tap water and 10%− 20% in 0.9% NaCl solution. Therefore, this superabsorbent polymer gel showed a high swelling property and stable salt resistance. It has a broad application prospect.

Development of antibacterial biocomposites reinforced with cellulose nanocrystals derived from banana pseudostem

Crystalline nanocellulose (CNC) was derived from banana pseudostem using acid hydrolysis method. CNC was characterized by scanning electron microscope, Fourier transformed infrared spectroscopy and X-ray diffraction. CNC was found in nanometric dimensions (18.79 ± 5.30 nm diameter and 202.12 ± 37.43 nm length) and exhibited high degree of crystallinity (81.67%). Chitosan-CNC based antimicrobial nanocomposites films were prepared by the incorporation of tetracycline and showed well-demarcated zone of inhibition against Staphylococcus aureus and Escherichia coli. Chitosan-CNC nanocomposite films showed significantly (p < 0.05) high tensile strength and good swelling properties in comparison to control CNC films. This study suggests that banana pseudostem can be used as a raw material for economic production of CNC and nanocomposites for biomedical applications.

Fabrication of self-healing pectin/chitosan hybrid hydrogel via Diels-Alder reactions for drug delivery with high swelling property, pH-responsiveness, and cytocompatibility

Self-healing hydrogels with pH-responsiveness could protect loaded drugs from being destroyed till it arrives to the target. The pectin-based hydrogel is a candidate due to the health benefit, anti-inflammation, antineoplastic activity, nontoxicity, and biospecific degradation, et al. However, the abundant existence of water-soluble branched heteropolysaccharide chains influenced its performance resulting in limitation of the potential. In the present study, we prepared a series of self-healing pectin/chitosan hydrogels via the Diels-Alder reaction. Moreover, pectin/chitosan composite hydrogel was prepared as a contrast. By comparison, it can be seen that the Diels-Alder reaction greatly improved the cross-linking density of hydrogels. The self-healing experiments showed excellent self-healing performance. In different swelling mediums, significant transformation in the swelling ratio was shown, indicating well-swelling property, pH- and thermo-responsiveness. The drug loading and release studies presented high loading efficiency and sustained release performance. The cytotoxicity assay that showed a high cell proliferation ratio manifested great cytocompatibility.

Development of bioresorbable smart injectable hydrogels based on thermo-responsive copolymer integrated bovine serum albumin bioconjugates for accelerated healing of excisional wounds

One of the major challenges in wound healing is the development of suitable hydrogels that are injectable, biocompatible with multiple functionalities and properties such as high mechanical, tissue adhesiveness, and swelling properties. However, these hydrogels should not elicit any immunological response and synthesis steps should be easier and tunable according to the requirements. Considering these properties, we synthesized a thermo-sensitive triblock copolymer consisting of bovine serum albumin (BSA) protein capable of leveraging the needs for a proper wound closure and tissue regeneration on excisional injuries. Firstly, the triblock copolymer consisting of poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide (PCLA) was synthesized and then the copolymer was grafted with BSA to yield BSA-PCLA bioconjugates. Aqueous solutions of free-flowing bioconjugate sol at room temperature can transform to gel at physiological temperature with high viscoelastic properties. Subcutaneous injection of BSA-PCLA bioconjugate sol into the back of Sprague-Dawley rats formed gel immediately and found to be bioresorbable after 5 weeks without significant toxicity at implantation sites. BSA-PCLA bioconjugate gel exhibited good adhesive property to major organs including liver, heart, and spleen when compared with control PCLA gel. When tested for in vivo wound closure trials, the BSA-PCLA gels showed rapid wound contraction compared to the PCLA and the control. The increased angiogenesis and collagen deposition were confirmed from the histological studies of the samples. These highly adhesive, biocompatible, biodegradable, thermos-sensitive bioconjugate gels show promising potential in wound healing and tissue regeneration without any additional biofactors or inorganic nanoparticles.

Radiation synthesis for a highly sensitive colorimetric hydrogel sensor-based p(AAc/AMPS)-TA for metal ion detection

The copolymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid AMPS with acrylic acid AAc using gamma radiation technique was performed in different co-monomer concentration. The effect of AMPS concentrations on the gel fraction (%) and swelling behavior were investigated. It is found that as AMPS concentrations increases both gel fraction (%) and swelling degree of p (AAc/AMPS) hydrogel samples increase. The p (AAc/AMPS) (1:2 ​wt%) hydrogel were selected to be loaded with tannic acid (TA) to give p (AAc/AMPS)-TA that are used as colometric sensor due to its high swelling properties (320 ​g/g). The colorimetric detection of 10 of (Mg2+, Cu2+, Co2+, Mn2+, Ni2+, Cr6+, Cd2+, Hg2+, Fe3+ and Sr2+) metal ions is run in water solution. The colometric sensor of p (AAc/AMPS)-TA hydrogel has been doped with 10 analyst with concentration range between 103−M and 5 ​× ​104−M for 60 ​min. The final deprotonated M+n-p (AAc/AMPS)-TA intermediate has shown a clear change in the color. The change in the color of M+n-p (AAc/AMPS)-TA is due to electron transition in the electronic properties of complex induced by Internal Charge Transfer (ICT) mechanism. The change in naked color eye and optical property where an obvious change in the vis-spectrum has been investigated. The obtained result showed a good selectivity of p (AAc/AMPS)-TA toward Cr6+ 10 fold than Hg2+. Therefore, the p (AAc/AMPS)-TA hydrogel could be proposed as a colometric sensor of determining the hazardous metal in solution.

Droplet-Based Bioprinting Enables the Fabrication of Cell-Hydrogel-Microfibre Composite Tissue Precursors

Composites offer an opportunity to couple particular benefits of their constituents to achieve unique material properties that can be of extra value in many tissue engineering applications. Strategies combining hydrogels with fibre-based scaffolds can create more biologically and structurally functional tissue constructs. However, developing efficient and scalable approaches to manufacture such composites is challenging. Here, we use a droplet-based bioprinting system called Reactive Jet Impingement (ReJI) to integrate a cell-laden hydrogel with a microfibrous mesh. The ReJI system uses microvalves that are connected to different bio-ink reservoirs and directed to continuously jet bio-ink droplets at one another in mid-air where the droplets react and form a hydrogel that lands on a microfibrous mesh. We produce cell-hydrogel-fibre composites by embedding human dermal fibroblasts at two different concentrations (5 x 106 and 30 x 106 cells/ml respectively) in a collagen-alginate-fibrin hydrogel matrix and bioprint it onto a fibre-based substrate. Our results show that both types of cell-hydrogel-microfibre composites maintain high cell viability and promote cell-cell and cell-biomaterial interactions. The lower fibroblast density thereby triggers cell proliferation whereas the higher fibroblast density facilitates faster cellular organisation and infiltration into the microfibres. Additionally, the fibrous component of the composite is characterised by its high swelling properties and quick calcium ions release. The data indicate that the composite constructs we create offer an efficient way to create highly functional tissue precursors for laminar tissue engineering in particular wound healing and skin tissue engineering applications.

Stabilization of Black Cotton Soil Using Portland Pozzolana Cement and GGBS - A Case Study

Soil is the most commonly available natural material. The knowledge of the soil mechanics has application in many field of civil engineering like foundation, underground and earth-retaining structures, Pavement design, Excavations, Embankments and Dams. The particular, to highway engineering, soil used as sub-grade, sub-base and sometime embankment material. Construction of road in a black cotton soil is a challenging task in the civil engineering field. The B.C. soils are expansive in nature and possess high swelling and shrinkage properties. Such soil can be stabilized using waste materials like fly ash, Ground Granulated Blast furnace slag, bagasse ash, pond ash etc to make the soil to be stable. As Ground granulated blast furnace slag is freely available, for projects in the vicinity of a Thermal Power Plant, the properties of expansive soil with cement and Ground granulated blast furnace slag in varying percentages can be used. In the present study laboratory studies are carried out on two combinations of black cotton soil and binders -10% stabilizing agent with soil (70% GGBS + 30% cement) and 20% stabilizing agent with soil (75% GGBS + 25% cement). The effect of various combinations on strength and durability has been discussed here. The main objective of this research work is to obtain an improved construction material by making the best use of available clayey soil and to make the effective utilization of Ground Granulated Blast Furnace slag and Portland Pozzolana cement.

Evidence that protein corona reduces the release of antimicrobial peptides from polymeric nanocapsules in milk

The antimicrobial peptide produced by Bacillus velesensis P34 has a broad activity against Gram-positive bacteria, showing potential as natural food preservative. In this work, nanocapsules (NCs) containing the peptide P34 were produced using the polymers poly-ε-caprolactone (PCL) or Eudragit RS-100 (EUD), and their antimicrobial activities were assessed evaluating L. monocytogenes growth in synthetic media, milk and isolated milk proteins. As results, cationic and anionic nanocapsules were obtained, with zeta potential ranging from +15 to +28 mV for EUD and around −19 mV for PCL, and average diameter in the range of 104–130 nm and 224–245 nm, respectively. In the antimicrobial tests, only the P34-EUD NCs presented activity against L. monocytogenes in BHI broth, possibly due to the EUD high swelling and permeability properties, as compared with PCL. In whole and skimmed milk, the P34-EUD NCs caused no inhibition of L. monocytogenes growth, due to a possible interaction of casein proteins with the NCs surface resulting in protein corona formation, which interfered with the antimicrobial peptide release. Therefore, the application of polymeric NCs as antimicrobial delivery systems in foods could be limited by the polymer type, and the adhesion of specific matrix proteins that could form protein corona, reducing the bioactive compound release.

In Situ Preparation of Novel Porous Nanocomposite Hydrogel as Effective Adsorbent for the Removal of Cationic Dyes from Polluted Water.

The use of some hydrogels as adsorbents for pollutants removal from wastewater is limited due to their high swelling properties and the difficulty in recovering them after the adsorption process. To overcome these problems, a new hydrogel nanocomposite based on chitosan-grafted-polyacrylic acid/oxidized electrospun carbon nanofibers (CT-g-PAA/O-ECNFs) was prepared by an in situ grafting polymerization process. The prepared hydrogel nanocomposite was used as a novel effective and highly reusable adsorbent for the removal of methylene blue (MB) from polluted water with low cost. The morphology and the structure of CT-g-PAA/O-ECNFs were investigated by numerous techniques. The effect of incorporating O-ECNFs on the swelling capability of the prepared hydrogel was explored in distillated water and MB solution at normal pH. The effect of parameters including ratio of O-ECNFs, contact time, pH, initial concentration, and temperature on adsorption process were explored. The adsorption isotherm and kinetic were studied by numerous non-linear models. The obtained results confirmed that the incorporation of O-ECNFs into the hydrogel network decreased the swelling capacity and improved its ability towards MB dye removal. The adsorption process depended on the pH value of the dye solution. Additionally, the adsorption and kinetic results were fitted using the Freundlich isotherm model and pseudo second order model (PSO), respectively. Moreover, the new adsorbents can be recycled for at least five cycles keeping its adsorption capacity and can be easily recovered without loss in its initial weight.

Nanosponges as protein delivery systems: Insulin, a case study.

Cyclodextrin-based nanosponges have been found to bepromising drug delivery systems. This paper investigates an application that still needs to be studied in depth, that is, the oral delivery of peptides and proteins, choosing insulin as a case study. The nanospongewas synthesized by crosslinkingβ-cyclodextrins withpyromellitic dianhydride, adopting a top-down approach for its subsequent formulation. Aphysicochemical characterization, in-vitro andin-vivo tests were carried out on the formulation developed. It was nanometric (around 250nm) with high negative zeta potential, mucoadhesion and swelling properties, good loading capability (about 14%) and encapsulation efficiency (above 90%). The in-vitro release of insulin was negligible at a gastric pH (below 2%) while sustained at an intestinal pH, thus showing a pH-sensitive behaviour of the nanosponge. The Caco-2 cell permeability assay proved that the intestinal permeation of insulin was enhanced when loaded inside the nanosponge. The in-vivo studies confirmed the presence of insulin in rat plasma and a marked hypoglycemic effect in diabetic mice after duodenal and oral administrations, respectively. These preliminary results are encouraging with a view to continuing to study this β-cyclodextrin nanosponge technology for the oral administration of insulin and extending this approach to other proteins of pharmaceutical interest.

Effect of 3D Printing Parameters on Self‐Driven Deformation Characteristics of Intelligent Hydrogel Actuators

AbstractBy changing printing parameters, a serious of hydrogel actuators with high mechanical strength and multiple deformation were successfully prepared through three‐dimensional (3D) printing. Base on the enhancement role of cross‐linking density of hydrogel inks, nanofibrillated cellulose (NFC) was treated as reinforcement phase to realize controllable regulation of rheology characteristics. With the increase of NFC content, stress and strain values of hydrogels increased gradually. Attributed to swelling rate and gel‐sol transition point, hydrogel with 12 mg/mL NFC was treated as 3D printing ink. The 3D printing parameters significantly affected self‐driven deformations. The hydrogel actuators with 0°/90°, 45°/135° and Archimedes chord configurations owned bending, spiral and twist deformations, respectively. The hydrogel actuators with relative larger length‐width ratio exhibited higher spiral degree. The high precise anisotropic swelling property of printed bilayer structure was the self‐driven deformation mechanism of hydrogel actuators. The combination of hydrogels and 3D printing extended the application of intelligent hydrogels.

Approach on chitosan/virgin coconut oil-based emulsion matrices as a platform to design superabsorbent materials

The design of innovative pharmaceutical products, able to reach unexplored market niches, requires natural materials use with improved swelling and moisture properties. Herein, chitosan (CHT), a natural polymer, was combined with virgin coconut oil (VCO), a resource extracted from coconut kernels, to develop emulsion-based films for biomedical purposes. The film's properties were tuned by changing VCO concentrations, and the structural, morphological, and physical properties of the films were evaluated. The CHT/VCO-based film morphology showed the presence of VCO droplets at different sizes, both in the surface and inner part. Moreover, the capability to develop CHT/VCO-films as superabsorbent materials was shown. The film extracts cytotoxicity was assessed using human adipose stem cells, and metabolic activity was confirmed. The findings suggest that incorporating a small volume of VCO into the CHT system, superabsorbent materials with the potential to be applied in biomedical devices that require high swelling properties, can be developed.

Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone and porous silk fibroin for meniscus tissue engineering

The meniscus has critical functions in the knee joint kinematics and homeostasis. Injuries of the meniscus are frequent, and the lack of a functional meniscus between the femur and tibial plateau can cause articular cartilage degeneration leading to osteoarthritis development and progression. Regeneration of meniscus tissue has outstanding challenges to be addressed. In the current study, novel Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone (PCL) and porous silk fibroin were proposed for meniscus tissue engineering. As confirmed by micro-structural analysis the entrapment of silk fibroin was successful, and all scaffolds had excellent interconnectivity (≥99%). The EiC scaffolds had more favorable micro-structure compared with the PCL cage scaffolds by improving the pore size while keeping the interconnectivity almost the same. When compared with the PCL cage, the entrapment of porous silk fibroin into the PCL cage decreased the high compressive modulus in a favorable matter in the wet state thanks to the silk fibroin’s high swelling properties. The in vitro studies with human stem cells or meniscocytes seeded constructs, demonstrated that the EiC scaffolds had superior cell adhesion, metabolic activity, and proliferation compared to the PCL cage scaffolds. Upon subcutaneous implantation of scaffolds in nude mice, all groups were free of adverse incidents, and mildly invaded by inflammatory cells with neovascularization, while the EiC scaffolds showed better tissue infiltration. The results of this work indicated that the EiC scaffolds of PCL and silk fibroin are favorable for meniscus tissue engineering, and the findings are encouraging for further studies using a larger animal model.

Lab scale Footing Analysis on Stabilization of Black Cotton Soil

Expansive soil implies low bearing capacity and high swelling property perhaps causes serious problems during construction includes low stability, non-uniform settlements and shear distribution. The soil stabilization is one such method to improve the process and it depends upon the soil condition and the nature of soil according to the desired requirements of footing. This study aims to increase the index and engineering properties of soil by addition of the natural fiber (sisal), lime and silica fume. Soil stabilization by lime involves the admixture in the form of calcium oxide or calcium hydroxide to the soil and silica fume as an industrial waste by product acts as a pore filling material. The project is economically viable because the stabilizing materials are easily available and less cost. This project is also analyzed by using the PLAXIS software.

Strength, Physicochemical and Morphological Characteristics of Fly Ash Stabilized Black Cotton Soil

Black Cotton (BC) soil is one of the problematic soil deposits in India. These soils are problematic due to their poor engineering properties and high shrinkage and swelling properties due to high affinity to water. This paper, explore stabilization of Black cotton soil having poor strength characteristic and high shrinkage and swelling characteristic, with Class F Fly Ash (FA) to verify its scope for use as soil sub-base construction material. Fly ash is a good soil stabilizing additive in alone or along with other additives. It improves the index and engineering properties of Black cotton soil as verified from previous research work. In this research work, a Laboratory experimental program was planned with Fly ash, variation from 0% to 50% and humid curing period varies from 0 to 28 days. In the first stage of the experiment, Atterberg’s limits and compaction test have performed on Black cotton soil with all mixture and found their respective Optimum Moisture Content (OMC) and Maximum Dry Densities (MDD). In the second stage of the experiment, UCS and CBR tests were carried out for immediate, 7, 14, 28 and 45 days curing periods. The Atterberg’s limits, OMC, MDD, UCS, and CBR of Fly ash stabilized Black cotton found much satisfactory at 20% FA and 28 days curing period. CBR and UCS value get increments of 77.91% and 83.45% respectively. From the physicochemical analysis through X-Ray Diffraction (XRD) and Scanning of Electron Microscope (SEM), it was noticed That enhancement of strength is due to the pozzolanic reaction which causes the formation of new crystalline mineral of Alumino-Silicate-Hydrates (ASH) and Calcium- Alumino-Silicate-Hydrates(CASH) in void space of the matrix.

Chitosan-Chondroitin sulfate based polyelectrolyte complex for effective management of chronic wounds.

Acute and chronic wound remain an unresolved clinical problem among various demographic groups. Traditional marketed products focus mainly on inhibition of bacterial growth at the wound site neglecting the tissue repair, which significantly affect the healing rate. It would be highly beneficial if a wound healing material can be developed which has both antibacterial as well as tissue regenerating potential. We have prepared a polyelectrolyte complex (PEC) using chitosan (CH) and chondroitin sulfate (CS) which can form an in-situ scaffold by spontaneous mixing. The fabrication of CH-CS PEC was optimized using Quality-By-Design (QbD) approach. The prepared PEC showed very high swelling and porosity property. It was found to be non-hemolytic with good blood compatibility and low blood clotting index. It also exhibited good antibacterial activity against both gram-positive and gram-negative bacteria. The cell proliferation study exhibited good cytocompatibility and almost four-fold increase in cell density when treated with CH-CS PEC compared to control. In summary, we demonstrated that the prepared CH-CS PEC showed good blood compatibility, high antibacterial effect, and promoted wound healing potentially by stimulating fibroblast growth, making it an ideal wound dressing material.

STUDYING THE EFFECT OF MULTICOMPONENT PICKLE ON THE QUALITY OF COOKED AND SMOKED HORSE MEAT PRODUCT

The multicomponent pumping pickle containing protein and carbohydrate components injected into the muscle tissue of horse meat, followed by massaging, tends to tenderize meat and improve the organoleptic characteristics of the finished product. Analysis of the data obtained showed that the introduction of additives to the multicomponent pickle contributed to the moisture-holding process in horse meat. An important physical and chemical indicator of meat products is the moisture-holding ability, which affects the consistency of finished products and the process of their microbiological spoilage since the amount of unbound moisture depends on the growth of the number of microorganisms. It is difficult to overestimate the importance of pH in meat technology, as the pH value determines the suitability of raw meat for processing. The maximum increase in the moisture-binding capacity of the control sample of horse meat up to 70% is achieved through 90 minutes of massaging, and then there is a decrease in the hydrophilicity of the system due to the physical destruction of muscle fibers. The pH value correlates with the moisture-binding capacity of raw materials and affects the safety of the product and its storage capacity. The relationship of pH and moisture- holding ability with the consistency of meat is not in doubt. In this regard, in the course of experimental studies, pH, moisture-holding ability and puncture voltage were determined. In Sample 1, which contained an extract from Goji berries, the water-holding capacity is increased by 3.4%. In Samples 2 (contained buckwheat flour) and 3 (contained an extract from Goji berries and buckwheat flour), with the combined use of buckwheat flour and goji berries, which demonstrated high swelling properties, the water-holding capacity increases by 6.1% and 7.2%, respectively additional 2.7% and 3.8%. The obtained data showed that the introduction of vegetable additives has a positive effect on the consistency of cooked-smoked meat product, in the prototypes on structural and mechanical properties, as well as shear force in the prototypes which has been reduced by 7, 13 and almost 20%, respectively.