Higher Soluble Solids Content Research Articles

Seedless okra pod, chlorophyll, vitamin C and mineral content development using plant growth regulator in vitro and in vivo culture.

Vegetables are rich in vitamins, minerals and dietary fiber that keep a significant role in the functioning of the human body to refrain human health benefits. The experiment was carried out to investigate the effect of different concentrations of IAA on the seedless pod, chlorophyll, vitamin and mineral content of okra as human health benefits. The innovative seed soaking method of application using 0, 25, 50, 100 & 200 mg/l of IAA concentrations was used in okra before germination and cultured in vitro and in vivo. The lower concentrations (25 and 50 mg/l) of IAA significantly increased the pod setting compared to the higher concentration (100 and 200 mg/l). The higher concentration (100 and 200 mg/l) had lower fruit settings than the lower concentration (25 &50) had higher fruit settings. The higher pod size was obtained in the concentration of 100 & 200 mg/l of IAA (34.18 cm²) as compared to the control and other concentrations. In addition, the highest soluble solid content was obtained by 100 and 200 mg/l of IAA concentration as compared to the other concentrations. The maximum vitamin C was found in the concentration of 100 mg/l of IAA as compared to the control and other concentrations. Moreover, higher mineral contents like K, Ca, Mg, Na and Fe were found in 100 & 200 mg/l of IAA. The higher concentrations (100 and 200 mg/l) of IAA greatly increased the seedless okra percentage as compared to the lower concentration. It seemed that 100 and 200 mg/l concentration IAA was a better concentration for mineral content and seedless okra production as compared to the other concentrations.

Investigation of Fruit Quality and Biochemical Traits of Rosehip (R. canina) Ecotypes in the Aegean Region of Türkiye

Rosehip is a valuable fruit species in particular for rural populations. In this study, fruit quality parameters of 15 wild grown rosehip ecotypes naturally obtained from seeds in the Aegean Region of Türkiye were determined. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma), soluble solid content, vitamin C, total phenolic, total carotenoid, total anthocyanin, total flavonoid and antioxidant capacity were investigated. The results showed great diversity among ecotypes. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma) and soluble solid content were between 2.28 and 3.29 g, 4.70 and 7.12 N, 69.34 and 81.67%, 0.97 and 1.07, 53.04 and 60.71 and 18.87 and 21.28%, respectively. The total antioxidant capacity was found to be 15.78–28.17 mg AAE/g in a DPPH assay. The vitamin C content of rosehip fruits was measured as 507–621 mg/100 g. Among ecotypes, A-15 gave the biggest fruits, A-1 had the highest soluble solid content and A-13 had the highest vitamin C content. These results suggested that some ecotypes showed more potent bioactive properties than other ecotypes, mainly related to the variations in the antioxidant capacity and bioactive content between ecotypes. Overall, this study provides additional insight into investigating the genotype exhibition of multifunctional bioactive properties.

Nutrient profiling of Wild Aonla (Emblica officinalis Gaertn.) populations in Northeast India: Assessing the potential of this fruit tree for ecological and human health restoration

Assessing the potential of native species like Emblica officinalis (Aonla) is vital for various reasons. It supports biodiversity conservation, sustainable agriculture, ecological equilibrium, human well-being, and climate resilience. This study, conducted across 30 diverse locations in Arunachal Pradesh, Northeast India, unveiled substantial morphological and nutrient variations among Aonla genotypes. Notable findings include G24's high total soluble solids content, G1's maximum acidity, and G13's elevated total carbohydrate content. G29 stood out with increased pectin, vitamin C, and phosphorus, while G19 exhibited higher total chlorophyll, carotenoids, and potassium. Additionally, genotypes displayed differences in crude protein, total starch, crude fibre, and total phenol content. Four promising genotypes (G29, G19, G24, and G1) hold the potential for genetic crop enhancement, offering benefits in local nutrition, health, soil quality, and water retention, not only in Northeast India but also beyond. This research underscores the significance of understanding and utilizing native resources to foster both ecosystems and human communities.

Bioactive profile and microbiological safety of Coffea arabica and Coffea canephora beverages obtained by innovative cold extraction methods (cold brews)

Coffee cold brews have been gaining prominence and popularity among consumers worldwide. However, only a few studies have systematically analyzed their chemical composition or evaluated microbiological safety aspects. This study aimed to evaluate the survival of Bacillus cereus and Escherichia coli in cold brews prepared from roasted and ground Coffea arabica and C. canephora seeds using the following preparation methods: immersion without filter (INF), immersion in a cotton filter bag (ICF), vacuum (Vac.) and cold dripping (Drip.). Traditional hot dripping methods using filter paper (HDFP) and cotton filter (HDCF) were also tested for comparison. Water at 4 °C or 25 °C was intentionally contaminated (105 CFU/mL) with cells of Escherichia coli ATCC 25922 (EC) and Bacillus cereus F4433 (BC) before coffee extraction and refrigeration at 4 °C. Coffee concentrations of 5, 10, and 15% were tested. Analyses of pH, soluble solids, nine chlorogenic acids and two lactones (CGA), caffeine, trigonelline, and melanoidins were performed. Results were compared by ANOVA, followed by the Fisher’s test, Pearson correlation, Variable Importance in Projection (VIP), and Cluster analyses, with a significance level of 5%. EC and BC were not detected (<10 CFU/mL and < 1 CFU/mL, respectively) after preparing C. arabica and C. canephora hot brews. In cold brews, the higher the extraction of soluble solids and bioactive compounds (with the highest occurring at 25 °C), the lower the counts of inoculated microorganisms during 24 h of storage. BC was not detected after 24 h of extraction and/or storage in the drinks obtained by ICF and Drip. at 5%, 10%, and 15% and INF and Vac. at 15%. EC was not detected in ICF and Drip. at 10 and 15%, and in INF at 15%. C. canephora brews exhibited higher levels of soluble solids, CGA, caffeine, and melanoidins than C. arabica brews. Based on these results, it can be concluded that in the absence of thermal processing as in hot brews, more concentrated cold brews, such as 15%, produced at 25 °C by dripping and immersion methods, are preferable for later dilution due to the higher content of soluble solids and bioactive compounds that contribute reducing the number of microorganisms in the beverage.

Amino acid-based biostimulant improves the productivity of Radish (Raphanus sativus L.) under saline stress

&#x0D; The radish culture is highly sensitive to salinity. The use of an amino acid-based biostimulant can, therefore, be an attenuator of the deleterious effect of salinity on the radish crop. The aim was to evaluate the effectiveness of a biostimulant based on amino acids and freshwater algae extract on radish (Raphanus sativus L.) cultivated under irrigation conditions with saline water. The experiment was carried out in a greenhouse. The saline levels evaluated were 0.15, 1.8, and 2.8 dS.m-1 and four doses of biostimulant were applied (0, 2, 4, and 6 mL/L). The statistical design was completely randomized, in a 4x3 factorial scheme with 4 replications. Biometric and biomass analyses, sodium and potassium contents, and soluble solids were performed. Was observed that plants submitted to high salinity levels (2.8 dS m-1) with application of 6mL/L of the biostimulant showed high values in height (12.2 cm), stem (5mm) and tuber (6mm) diameter, leaf area (50cm2), fresh and dry mass of aerial part (3.5 g) and underground system (5.8 g). The application of the biostimulant 6 mL/L, at the salinity level of 2.8 dS.m-1, favored the increase of saline ions in the tuber of the plants. The application the biostimulant provided a higher content of soluble solids. Applications of the biostimulant in plants with saline stress showed beneficial responses in the performance of the radish, therefore the dose of 6 mL/L is recommended for a satisfactory response of the performance of the radish in saline conditions.

Maturation indexes and physiological quality of seeds and fruits of Morinda citrifolia L.

The seeds of Morinda citrifolia L. present unevenness and delay in germination in relation to other fruit trees. Knowledge about the physiological quality of seeds is extremely relevant for the production of seedlings and propagation of the species. Thus, the objective was to study maturation indices and the physiological quality of seeds and fruits of M. citrifolia. Seeds from freshly picked fruits, fruits harvested after seven days of maturation and freshly dropped fruits were used. The extracted and processed seeds were submitted to the following pre-germination treatments: GA3, KNO3 and H2O. The following were analyzed: fruit length and diameter, seed length, width and thickness, number of seeds per fruit, average number of seeds per kg, weight of 1000 seeds, germination, germination speed index, shoot and root length, seedling dry mass and percentage of normal seedlings. The experimental design used was completely randomized, with three stages of fruit maturation and six treatments, with four replications of 25 seeds. M. citrifolia seeds do not show tegumentary dormancy. The seeds of freshly harvested fruits and fruits harvested after seven days of maturation do not need pre-germination treatments. GA3 promotes greater germination in seeds of fruits harvested from the ground. Fruits that have just fallen to the ground have a higher content of soluble solids and a higher content of acidity. The storage of fruit in a refrigerator for a week provides the maintenance of texture characteristics, such as hardness, adhesiveness, elasticity and gumminess. Fruits with greater hardness have higher physiological seed quality.

Barley and Malt as Base Ingredients for the Production of New Bio-Functional Foods

This work aimed to evaluate the chemical composition, bioactive compounds (phenolics and γ-aminobutyric acid, GABA), and antioxidant properties of different barley varieties (Overture, Charles, Sinfonía, Montoya, and Andreia) and their malts to weigh up them as potential ingredients for producing new bio-functional foods. For this, five barleys and five malts obtained from them were studied. Regarding chemical composition, total starch was the main component (≈62%) of barleys followed by total dietary fiber (≈22.6%) and proteins (≈9.5%). Potassium and phosphorus were the most abundant elements, with mean values being 3746.1 and 3679.1 g 100g&lt;sup&gt;-1&lt;/sup&gt;d.w., respectively. Regarding the free amino acid profile, the proportion of hydrophobic free amino acids was higher than that of branched-chain amino acids or sulfur-containing amino acids and the mean value of GABA was 8.8 mg 100g&lt;sup&gt;-1&lt;/sup&gt;. Ferulic acid was the most abundant free phenolic acid detected in the different barleys, followed by coumaric acid. All barley extracts showed ABTS and DPPH inhibitory activities and ferric-reducing antioxidant power (FRAP). As expected, total starch, total dietary fiber, and crude fat contents of malts were lower than those found for barley. However, the malting process increased GABA, ferulic acid, hydrophobic free amino acids, branched-chain amino acids, and sulfur amino acid contents. Additionally, the antioxidant properties of malts were higher than those obtained for barleys. Barley flour could be successfully used as a bio-functional ingredient in many foods. Furthermore, given the high content of soluble solids (mainly carbohydrates, antioxidant compounds such as free phenolic acids and free amino acids, and GABA), malted flours would be novel ingredients for preparing beverages with bio-functional properties.

Long-term field evaluation of Pera sweet orange selections under the Brazilian humid subtropical climate

Currently, a low number of citrus varieties are commercially planted to supply the Brazilian citrus industry demand. The diversification of citrus scion and rootstock varieties is an important strategy to prevent disease outbreaks and to obtain higher off-season profits, as well as to extend fruit supply for the citrus processing industry. The aim of this study was to evaluate 16 Pera sweet orange selections (Citrus ×sinensis Osbeck) under a humid subtropical climate in the northwest region of the state of Paraná, Brazil regarding tree development, production, and disease incidence. The experimental orchard was planted in December 2012 in a randomized block design with three replications and five trees per plot. All scions were grafted on Rangpur lime rootstock (C. ×limonia Osbeck). The evaluations were performed from 2016 through 2022, and included vegetative growth, yield, fruit quality, estimated tree density and productivity for new plantings, and incidence of diseases as citrus canker, huanglongbing (HLB), and citrus tristeza virus (CTV). All data was submitted to ANOVA and means grouped by the Scott Knott’s test (p ≤ 0.05). Pera Perão was the most vigorous variety, with the heaviest and largest fruit, and was among the selections with the highest content of soluble solids (SS), titratable acidity (TA) and juice. However, this variety showed a light-yellow juice color, and one of the lowest SS·TA−1 ratio. This selection had the lowest percentage of fruits with citrus canker but was among the selections with the highest HLB incidence. The selections Ovale, Clone 9, and Clone 42 also showed higher vegetative growth and fruit length, but lower SS content, juice yield and technological indices. All other Pera selections had lower tree height and canopy volume than Ovale, Clone 9, and Clone 42, and adequate overall fruit quality. Conversely, Pera Comprida, Dibbern, Morretes, and Seleç ão 12 scored lower cumulative fruit yield. Pera Pirangi and Comprida had the highest citrus canker incidence on fruits, while Morretes, Ovo, Pirangi, Seleç ão 12, and Seleç ão 27 had the highest HLB incidence alongside Perão. Low severity level of stem pitting was observed within the evaluated selections. The scores ranged from one (absence of stem pitting) to two (sparse surface stem pitting). IAC, Olímpia, Mel, Bianchi, and IPR 159 revealed to be the most suitable for new plantings under humid subtropical edaphoclimatic conditions, as trees had small vegetative growth, high yield performance and fruit quality, in addition to showing lower incidences of citrus canker, HLB and CTV.

Characterization of Tomato Genotypes for Important Fruit Quality Traits

Characterization of Tomato Genotypes for Important Fruit Quality Traits

Characterization of bioactive and fruit quality compounds of promising mango genotypes grown in Himalayan plain region.

Twenty mango genotypes grown in the plains of the Himalayas were characterized by their physical, physiological, biochemical, mineral and organoleptic attributes: fruit firmness, weight, peel thickness, shape, dry seed weight, respiration rate, weight loss, and shelf life. Biochemical attributes such as soluble solids, total carotenoids, total phenolic content, antioxidant activity, titratable acidity, ascorbic acid and total sugars were also determined. In addition, mineral content and fruit-softening enzymes were measured, and an organoleptic evaluation was performed. Polygalactouronase (PG), pectin methylesterase (PME) and lipoxygenase (LOX) were measured from the pulp adjacent to the peel. Similarly, biochemical attributes and mineral content were evaluated using fruit pulp, while organoleptic evaluation included fruit pulp characters and the fruit's external appearance. The results of the study showed that the 'Malda' genotype exhibited the highest total phenolic content (560.60 µg/100 g), total antioxidant (5.79 µmol TE/g), and titratable acidity (0.37%) among the tested genotypes. 'Amrapali' had the highest soluble solid content (25.20 °B), 'Jawahar' had the highest ascorbic acid content (44.20 mg/100 g pulp), 'Mallika' had the highest total flavonoid content (700.00 µg/g) and 'Amrapali' had the highest total carotenoid content (9.10 mg/100 g). Moreover, the genotypes 'Malda', 'Safed Malda'and 'Suvarnarekha' had a shelf life of 4-5 days longer than other tested genotypes. The genotypes with high biochemical attributes have practical utility for researchers for quality improvement programmes and processing industries as functional ingredients in industrial products. This study provides valuable information on the nutritional and functional properties of different mango genotypes, which can aid in developing improved varieties with enhanced health benefits and greater practical utility for processing industries.

The results of the evaluation of the biochemical composition of raspberries in the conditions of the Orenburg Region

This article presents the results of studies of the biochemical composition of raspberry varieties (Rubus idaeus L.) cultivated in the Orenburg region. The research was carried out on the collection plots of raspberries of the Orenburg branch of the Federal State Budgetary Research Center of Horticulture 2005-2021. The high content of soluble solids in summer raspberries, compared with the control variety Rannii Syurpriz (12.17-15.21 %), had the varieties Samarskaya Plotnaya, Juravlik, Solnishko and on average exceeded by 3.6-5.8 %, and in raspberries of the repair type of fruiting, the highest value of this indicator was noted in varieties Oranjevoe Chudo (10.7-13.0 %) and Arisha (11.4-12.8 %). The coefficient of variation is 11-16 %. On average, the sugar content in the fruits of raspberry varieties varied from 5.9 to 8.5 %, with the largest amount in the fruits of the varieties Rannii Syurpriz (K) (8.5 %), Juravlik (8.2 %) and Solnishko (8.1 %). These varieties had the most balanced taste due to the low acid content. The maximum content of titrated acid in fruits was 2.0 % in Samarskaya Plotnaya varieties and 2.1 % in Rubinovoe Ojerele varieties. Over many years of research, it has been revealed that the content of ascorbic acid in summer raspberries averaged 24.9 (Volnica) – 51.1 (Skromnica) mg/100 g, and in raspberries of the primocane raspberry of fruiting 40.0 (Jar-Ptica) – 45.5 (Gerakl) mg/100 g. A higher content of ascorbic acid in the fruits of summer raspberries, compared with the control variety Rannii Syurpriz (36.19-45.14 mg/100 g), were characterized by the varieties Skromnica (44.68-58.3 mg/100 g), Samarskaya Plotnaya (39.04-60.48 mg/100 g), Blestyaschaya (35.16-61.32 mg/100 g), Juravlik (33.37-56.90 mg/100 g). The highest content of ascorbic acid in the fruits of raspberries of the repair type of fruiting was found in the varieties Gerakl (41.3-49.6 mg/100 g) and Arisha (40.5-47.3 mg/100 g) in comparison with the control variety Jar-Ptica (35.3-44.7 mg/100 g). Th us, varieties have been selected from the genetic collection of raspberries according to a complex of biochemical characteristics for use in the breeding process: Jar-Ptica, Juravlik, Arisha.

Agrobiological and consumer assessment of new cherry varieties of Dagestan breeding

The development of new varieties of cherries with a combination of economically important traits, along with the high nutritional value of the fruit, is an important trend in breeding research, which renews and expands the variety range of cherries. The agrobiological and consumer properties of seven new varieties of sweet cherries of Dagestan breeding (FSBSI Dagestan breeding experimental station of fruit crops) are presented: Bela, Dagestan red, Bigarro Krainsky, Garnet, Lyubimitsa Korvatskogo, Lezginka and Nike. The research was carried out using conventional methods for a variety research and biochemical methods of analysis. It was determined that the cherry varieties Lyubimitsa Korvatskogo, Pomegranate, White and Nike are highly resistant to spring frosts, with the subfreezing of flowers being less than 18.3%. The fruits of the varieties Nike, Bela, Pomegranate, Lezginka and Lyubimitsa Korvatskogo are the most resistant to dehiscence, since the index of dehiscence during ripening at high humidity for these varieties varied within 13.5–18.2 %. The susceptibility of new varieties to Coccomyces and monilial blight is insignificant (no more than 1.2 points). Varieties Bigarro Krainsky, Pomegranate, Lezginka and Nike are characterized by the highest content of soluble solids (12.4–16.3%) and sugars (11.01–12.02%) in fruits. Bigarro Krainski, Bela and Lezginka exhibited the highest vitamin C content (9.24– 9.70 mg %). With the exception of the Bela variety (5.0 g), all new varieties of cherries are characterised by large fruits (7.5–9.5 g). Tasting indicators were rated as high (within 4.7–5.0 points on a five-point scale) for the Lyubimitsa Korvatskogo, Pomegranate, Lezginka and Nike varieties. The average yield was the highest for Nike, Bela and Garnet (8.0–9.6 t/ ha). The study showed that new cherry varieties obtained a combination of economic and biologically valuable traits, along with improved consumer properties compared with the control varieties. Therefore, they are recommended as valuable parent materials for breeding programs and the expansion of commercial cherry orchards in Dagestan in order to obtain high-quality fruit products.

Diversity of plums belonging to P. domestica L., P. insititia L. and Prunus × rossica Erem. Tree vigour, yielding and fruit quality attributes

From 2017 to 2022, tree growth, yield components, fruit quality attributes and antioxidant capacity of autochthonous and rare plum cultivars of different genetic origin were investigated. The research was carried out in the environmental conditions of Serbia in orchard where intensive horticultural measures were applied. The results showed a wide variability of the examined parameters among cultivars and among plum types. The ‘Olenka’ plum had the most vigor trees, whereas ‘Saint Julien’ had dwarf trees. The Serbian autochthonous plums ‘Metlaš’ and ‘Crvena Ranka’ had the best yield components (Y, CY, YE). ‘Timočanka’ had the highest FW and fruit size, whereas ‘Metlaš’ and ‘Saint Julien’ had the weakest fruit physical characteristics. Unexpectedly, ‘Saint Julien’ had the highest content of soluble solids, both ripening and sweetness indexes and RS. The highest and similar content of TS was found in the fruits of ‘Crvena Ranka’, ‘Timočanka’ and ‘Obilnaya’. ‘Crvena Ranka’ and ‘Obilnaya’ had the highest content of N-RS. The Czech autochthonous cultivars ‘Durancie’ and ‘Chrudimská’ had a similar and highest content of vitamin C, while the highest TPC and TFC were found in the fruits of ‘Metlaš’ and ‘Olenka’, respectively. The highest amount of anthocyanins had ‘Obilnaya’, whereas the lowest had ‘Saint Julien’. Fruits of ‘Durancie’ had the highest TAC, followed by ‘Timočanka’ and ‘Obilnaya’, whereas fruits of ‘Olenka’ had the poorest antioxidant power. Considering their overall performances, evaluated plums can be grown in production orchards for different purposes or can be an alternative to the leading commercial cultivars in similar ecological conditions. Also, they can be used for breeding programs.

Characterization of ‘Wonderful’ pomegranate in the state of Chihuahua, México

The consumption of fruits rich in antioxidants has increased in recent years, a clear example of this is the pomegranate (Punica granatum L.). The cultivation of the pomegranate shows great expectations due to its profitability and its adaptability to development in arid zones. In this research, ‘Wonderful’ pomegranate fruits from Coyame Chihuahua, México were used. Quality parameters and bioactive compounds were evaluated to characterize the pomegranate produced in the state of Chihuahua, six different lots from local producers were used. The results showed significance between the different treatments, lot 5 (L5) presented the best quality characteristics such as: weight, diameter, lower percentage of shell and cartilage, as well as one of the highest percentages of arils, in addition, it had a high Total Soluble Solids (TSS) content, a high Titratable Acidity (TA) and a low sugar-acidity ratio, however, lot 3 (L3) showed the highest antioxidant capacity. In general, the qualities and attributes of the Chihuahua pomegranates, obtained higher values ​​in most of the evaluated variables in comparison with other reported results. On the other hand, as a result of this research, the implementation of the color index in the peel is proposed as a tool for the prediction of the maturity index of the pomegranate. This study contributes to the producers of this fruit tree because there is little information on the production and characterization of the pomegranate.

Physicochemical characteristics and antioxidant capacity of Ecuadorian paramo flowers

Ecuador is a megadiverse country with a wide variety of floral species that have been little studied. In this context, the study's objective was to evaluate the physicochemical characteristics and the antioxidant activity of several floral species of paramo of Pichincha Province in Ecuador. Thus, the weight, size, color, pH, soluble solids, moisture and ash of fresh flower was quantified. In addition, carotenoids, phenolic compounds and antioxidant activity were quantified in lyophilized powder. The results obtained showed that the flowers of Werneria nubigena were the longest (43,80 cm); Brugmansia x candida the widest (9,88cm) and heaviest (9,22g); Tristerix longebracteatus presented high soluble solids content (21,5 °Brix), Lupinus microphyllus high pH (14,00), Ceanothus maritimus high titratable acidity (0,26%), Castilleja integrifolia high ash content (6,42%) and Bidens ferulifolia high moisture content (95,73%). In addition, the highest ranges of total carotenoids and total phenolics were presented by yellow Bidens ferulifolia (24,81 µg β-carotene/g PS) and Fuchsia vulcania (531,77 mg EAG /g PS), respectively. Finally, it was found in Bomarea multiflora high values of antioxidant capacity (182,08 trolox eq. µmol/ g PS). These results suggest that the paramo flowers contain essential bioactive compounds that could be used for food, medicinal and cosmetic purposes. Keywords: Bioactive compounds, carotenoids, phenolic compounds, Andean flowers

A 21-bp InDel in the promoter of STP1 selected during tomato improvement accounts for soluble solid content in fruits.

Domestication and improvement are important processes that generate the variation in genome and phonotypes underlying crop improvement. Unfortunately, during selection for certain attributes, other valuable traits may be inadvertently discarded. One example is the decline in fruit soluble solids content (SSC) during tomato breeding. Several genetic loci for SSC have been identified, but few reports on the underlying mechanisms are available. In this study we performed a genome-wide association study (GWAS) for SSC of the red-ripe fruits in a population consisting of 481 tomato accessions with large natural variations and found a new quantitative trait locus, STP1, encoding a sugar transporter protein. The causal variation of STP1, a 21-bp InDel located in the promoter region 1124bp upstream of the start codon, alters its expression. STP1 Insertion accessions with an 21-bp insertion have higher SSC than STP1 Deletion accessions with the 21-bp deletion. Knockout of STP1 in TS-23 with high SSC using CRISPR/Cas9 greatly decreased SSC in fruits. In vivo and in vitro assays demonstrated that ZAT10-LIKE, a zinc finger protein transcription factor (ZFP TF), can specifically bind to the promoter of STP1 Insertion to enhance STP1 expression, but not to the promoter of STP1 Deletion , leading to lower fruit SSC in modern tomatoes. Diversity analysis revealed that STP1 was selected during tomato improvement. Taking these results together, we identified a naturally occurring causal variation underlying SSC in tomato, and a new role for ZFP TFs in regulating sugar transporters. The findings enrich our understanding of tomato evolution and domestication, and provide a genetic basis for genome design for improving fruit taste.

Performance of Sweet Cherry Cultivars and Advanced Selections on Gisela 5 Rootstock in Young Orchards.

Six sweet cherry cultivars and two advanced selections of Gisela 5 rootstock were tested in 2015-2021 at the Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry. Fruit trees were planted at distances of 4.5 × 2.5 m and trained as spindles. Orchard floor management included frequently mown grass in alleyways with herbicide strips along tree rows. Cultivars 'Mindaugė' and 'Irema BS' were the most vigorous at the end of the seventh leaf. Their trunk diameter achieved 11.6 cm. The 'Merchant' cultivar had the smallest trunk diameter-9.3 cm. The average yield in 2018-2021 ranged from 2.75 t/ha for 'Vega' to 8.73 t/ha for 'Regina'. Cultivars 'Regina', 'Sunburst', 'Irema BS' and 'Merchant' had the highest cumulative yield efficiency of 0.440-0.503 kg/cm2 with respect to the trunk cross-section area (TCSA). The least productive cultivar 'Vega' produced fruits of the highest average weight-9.9 g. Fruits of 'Regina' and 'Sunburst' were large as well-8.8-9.1 g. 'Irema BS' fruits had the highest soluble solids content (SSC)-20.2%. The lowest SSC was recorded in 'Merchant' and 'Sunburst' fruits-14.7-15.8%. The yield of advanced selection, No. 102, equaled to the yield of cv. 'Regina'. No. 102 had a high fruit weight, and fruits were distinguished by attractiveness and taste.

Fruit quality of strawberry cultivars submitted to nutrient spraying in semi-hydroponic system

Society's demand for foods with high organoleptic and nutraceutical quality requires studies that identify superior cultivars and nutritional management that provides gains in quality attributes. The attributes pH, soluble solids (SS), titratable acidity (TA), SS/TA ratio, total contend of flavonoids (FVT), anthocyanins (ATC), phenols compounds (TPC), vitamin C (Vit C) and total antioxidant activity (TAA) of pseudofruits of two cultivars of short days (Pircinque and Jônica) and two of neutral days (Albion and San Andreas) submitted to spraying with water or nutrients (PAN) (B, Zn and B + Zn) under semi-hydroponic system in Alto Vale do Itajaí/SC, Brazil. Pseudofruits from short-day cultivars have higher SS content and SS/AT ratio. PAN does not influence pH, SS, AT and SS/AT ratio. Strawberries from Jônica and Albion stand out for their high Vit C content. The application of Zn increases Vit C of the cultivars. The variation found in the levels of FVT, ATC and TAA, is mainly due to the genetics of each cultivar. The TAA correlated positively with the FvT and ATC content and negatively with SS. Higher TAA were observed in decreasing order for San Andreas, Jônica, Albion and Pircinque. Pircinque strawberries are sweeter, with higher SS and SS/AT ratio, but with lower TAA.

Identification of Genes of Molecular Marker TGS0892 on Chromosome 6 and Its Mechanism of Soluble Solids Metabolism in Tomato

Tomato (Solanum lycopersicum L.) is one of the vegetables widely cultivated in the world, whose fruits are rich in nutrients. Soluble solids content (SSC) is one of the important factors affecting tomato fruit flavor and plays a decisive role in improving tomato quality. Molecular markers are genetic markers that reveal plant genetic polymorphism at the DNA level, which have the advantages of improving breeding purposes, increasing selection efficiency, and shortening breeding years. The molecular marker TGS0892 is located on chromosome 6 of the tomato genome and is closely related to soluble solids. In the present work, five different tomato cultivars were used as experimental materials. The results showed that ‘TD-10’ had the lightest single fruit weight and the highest soluble solids content, while ‘TD-8’ and ‘TD-9’ had heavier single fruit weight and lowered soluble solids content. Seventeen genes within 50 kb upstream and downstream of the molecular marker TGS0892 were identified using bioinformatics methods, and their structural analysis and functional annotation were performed. Quantitative reverse transcription PCR (RT-qPCR) showed that the expression levels of the 17 genes in different tomato cultivars were classified into two major categories, with the highest expression in ‘TD-7’ and other cultivars, respectively. Soly065970 and Soly066010 were significantly more expressed in high soluble solids tomato cultivars (‘TD-7’ and ‘TD-10’) and less expressed in the low soluble solids tomato cultivar (‘TD-9’). The results suggested that Soly065970 and Soly066010 may be involved in regulating the soluble solids metabolic process, which provides a reference for studying the formation mechanism of highly soluble solids in tomatoes.

Production and oenological potential of Riesling variety clones 49, 1091 and 1089

Clonal selection in viticulture is based on genetic variability with the aim of creating superior plants (clones) that enable more economical production. The Riesling variety, with a long tradition of cultivation, has been the subject of extensive clonal selection and clones 49, 1089 and 1091 were created in France. During the two-year period (2016-2017), the productivity and oenological potential of Riesling clones 49, 1089 and 1091 in the agroclimatic conditions of Bosnia and Herzegovina were conducted. Significant differences between clones were found in the yield per vine (1.34-1.87 kg), ten skins weight (2.17-2.46 g) and mass of ten berries seeds (0.27-0.35 g). The highest soluble solids content was found in clone 49 (22% Brix). Significant variation in the content of total polyphenols in the grape skin was observed in clones 49 and 1091 during the research period, compared to clone 1089 where the differences were less pronounced. The tested clones statistically differed each other significantly in most of the tested wine characteristics, except for the content of volatile acids. Clone 49 had slightly better results in terms of yield indicators, as well as a more uniform yield compared to clones 1089 and 1091. The tested clones, grown in agroecological conditions of northwestern part of Bosnia and Herzegovina showed satisfactory results in terms of grape and wine quality, which do not lag the Riesling variety in terms of literary data.