This study aimed to explore the practical effect and reasonable evaluation methods of specialized high quality nursing care in operating room. Stratified random sampling was used to select 93 nurses from the operating rooms of two affiliated hospitals of Kunming Medical University in Yunnan Province. A set of “Specialized High Quality Nursing Care Items in Operating Room” was implemented in the operating rooms of the two hospitals simultaneously. The “Task Performance and Contextual Performance Scale” was used to measure and collect data of nurses in operating room before and after the implementation of the items. After the “Specialized High Quality Nursing Care Items in Operating Room” was implemented, the task performance total score and dimension scores of patient care, nursing information provision and specialized nursing skills were (58.59±1.77), (19.27±1.16), (19.50±1.11), (19.80±0.70), respectively, higher than the respective scores (37.21±1.92), (5.55±0.70), (13.78±1.12), (17.87±0.98) before the implementation, and the differences were statistically significant (t=−82.48 to −15.15, P<0.01). After the “Specialized High Quality Nursing Care Items in Operating Room” was implemented, the contextual performance total score and dimension scores of mutual support among colleagues, extra burden outside work tasks, and support to the organization were (70.43±2.94), (28.23±1.21), (18.52±1.29), (23.67±1.86), respectively, higher than the respective scores (36.20±1.79), (15.52±1.94), (7.33±1.11), (13.34±0.87) before the implementation, and the differences were statistically significant (t=−91.99 to −46.68, P<0.01). In conclusion, the “Specialized High Quality Nursing Care Items in Operating Room” is a series of nursing care that can improve task performance and contextual performance of nurses in operating room and promote the quality of high-quality nursing in operating room. The task performance and contextual performance can also comprehensively and effectively evaluate the implementation effect of specialized high quality nursing care in operating room.