Recent advancements in 3D printing involve reinforcing the polymer matrix with high-strength fibers offer improved mechanical properties over unreinforced polymeric materials. The fiber-reinforcement offers a great potential to customize fiber-reinforced 3D printed polymer composites for complex shapes and high-performance parts for diverse end use applications. The advance capabilities also allow to vary the 3D printed composite weight without compromising the mechanical properties. The aim of this research was to investigate unique macrostructures of 3D printed samples developed by varying the printing parameters, and their influence on the tensile behavior. Samples developed for this study comprised of nylon matrix reinforced with continuous fiberglass with three varying fiber orientations and four different infill geometries. Tensile properties of the unreinforced and reinforced 3D printed samples were evaluated to investigate the impact of fiber orientation and infill geometries and further benchmarked with traditional 3D orthogonal woven (3DOW) composites. The results indicated that the tensile properties due to fiber orientation mainly dictated the tensile properties more than the cellular structure. The analyses from this study showed that the macrostructure (hence tensile properties) of 3D printed specimens were directly related to the printing parameters that include fiber amount and orientation, void formation and the bonding between individual raster/layers.