Highest Percentage Of Inhibition Research Articles

DETERMINATION OF THE ANTIPROLIFERATIVE EFFECT OF STERNBERGIA LUTEA (L.) KER GAWL. EX SPRENG. EXTRACTS ON A375 MALIGNANT MELONOMA CELL LINE

Epidemiological evidence confirms that plants are primary sources of drugs used to reduce the incidence of cancer and prevent cancer-related deaths. Sternbergia species are used for therapeutic purposes due to the amaryllidaceae alkaloids, lectins, phenolic acids, pigments, and volatile components they contain. In this study, the anticancer properties of S. lutea extracts were tested on the A375 malignant melonoma cell line. In addition, in the study, the transcriptional expression of BCL-XL and Cas9 genes, which function in cell proliferation and apoptotic pathways, in cells treated with plant extracts were determined by qRT-PCR. According to the cytotoxicity results made by the MTT test, the highest inhibition percentage was determined at the plant's concentration of 500 μg/𝑚L. At this concentration, A375 cells were inhibited by 83.63%, and the IC50 value of the extract was calculated as 194.64 μg/𝑚L. In addition, in qRT-PCR analyses, a statistically significant increase was observed in the mRNA expression levels of Cas9 genes, which are positively correlated with the apoptotic pathway, in the extract and cisplatin-applied groups compared to the control group.

Preclinical Evaluation of the Anti-inflammatory Properties of Plants Extracts of Ficus exasperata (Moraceae) on Wistar Rat Models

Introduction: In view of the rising global death rate associated with inflammatory diseases, a cost friendly, more effective and, safer drug with lesser side effects is needed. Inflammation is a part of the complex biological response of vascular tissue to harmful stimuli, such as pathogens, damaged cells, or irritants. It is characterized by redness, swollen joints, joint pain, heat, and loss of joint functions. Chronic inflammatory diseases have been recognized as the most significant cause of death in the world today, with more than 50% of all deaths being attributable to inflammation-related diseases. Non-steroidal anti-inflammatory drugs (NSAIDs) and Steroidal anti-inflammatory (SAIDs) are used throughout the world for the treatment and management of inflammation, pain, and fever. Since many NSAIDs are associated with, side effects such as gastrointestinal bleeding and suppressed function of the immune system, medicinal herbs are new alternatives to search out new chemical substances that would have better therapeutic results with low toxic profile. Recent studies on Ficus exasperata suggest that it might be as effective as some NSAIDs in the treatment of inflammation and related pain. Objectives: To evaluate the anti-inflammatory properties of the different plants extracts of Ficus exasperata in rats of Wistar train. Methods: The powdered material of leaves of Ficus exasperata was extracted by decoction, infusion, aqueous maceration, and hydroethanolic maceration. Phytochemical screening was carried out on the four different extracts: aqueous extract, hydroethanolic extracts, decoction and Infusion. The anti-inflammatory properties of Ficus exasperata were evaluated in vitro by egg albumin and bovine albumin using the heat induced Protein denaturation assay. Results: The leaves of Ficus exasperata was effective in inhibiting albumin denaturation. Varying concentrations of the plant extract significantly, (p < 0.05) inhibited the denaturation of albumin when compared to the control group. Aspirin and Indomethacin showed a similar trend. The inhibition by the extract is concentration-dependent with 32.25 µg/ml having an inhibition of 8.89 for infusion, 9.66% for hydroethanolic, 10.29% for aqueous maceration, and 12.6% for decoction compared to indomethacin and aspirin with inhibition 36.94% and 40.36%, respectively. The highest percentage inhibition was seen at 1000 µg/mL with inhibition of 34.22% for aqueous maceration, 40.77% for infusion, 41.61 % for hydroethanolic and 42.34% for decoction compared to indomethacin and aspirin with 67.06% and 58.45%, respectively. The plants extracts showed anti-inflammatory activity by decreasing the rate of denaturation of protein. Conclusion: The studied extract justifies anti-inflammatory properties, which were confirmed by protecting against protein denaturation.

Spectrophotometric Detection of Beta-Lactamase Inhibition by Plant Extracts

Background: Antibiotic-resistant bacteria have been spreading with increasing frequency over the past several decades. The decreasing effectiveness of therapies in treating bacterial resistance, due to the production of beta-lactamase (EC.3.5.2.6), has led to combining beta-lactamase inhibitors with commonly used beta-lactam antibiotics. Phytochemical compounds produced by plants have varied biological properties such as antioxidant, antimicrobial, antidiabetic, anti-inflammatory, anticancer, antihypertensive and others. Polyphenolics, flavonoids, coumarins, alkaloids, tannins, steroids and terpenoids are the main phytochemicals found in plants extracts. This study aims to assess the inhibitory activity of Annona senegalensis Pers., Terminalia superba Engl. & Diels, Ipomoea batatas Lam., and Psidium guajava L. against a Bacillus cereus beta-lactamase and four beta-lactamases extracted from clinical isolates of Escherichia coli. Methods: Beta-lactamase inhibition was measured using a spectrophotometric assay with benzylpenicillin as the substrate, by a modified acidimetric method. Clavulanic acid was used as the standard beta-lactamase inhibitor. Subsequently, the inhibition of the hydrolysis of other commonly used beta-lactam antibiotics was assessed using a UV spectrophotometric method. Results: Among the tested extracts, Ipomoea batatas showed no effect on the beta-lactamases, whereas Terminalia superba and Annona senegalensis exhibited inhibitory activity against all the beta-lactamases. Psidium guajava inhibited two of the five tested enzymes. The highest percentage of inhibition was observed with Terminalia superba on the beta-lactamase of Bacillus cereus, reaching 75.73%. Regarding the combination of beta-lactam antibiotics with plant extracts, the combination of Terminalia superba and ceftriaxone showed the highest inhibition, with a percentage of 64.90%. The combination of Annona senegalensis and ceftazidime was the least effective, with an inhibition rate of only 12.95%. Conclusion: Two of the tested ethanolic plant extracts showed interesting beta-lactamase inhibitory effects and could contribute to the development of more effective therapies against beta-lactamase-producing bacteria by combining natural inhibitors from plants with existing beta-lactam antibiotics.

Potensi Bakteri Simbion Rayap sebagai Agens Pengendali Hayati Penyakit Rebah Semai (Sclerotium rolfsii) pada Tanaman Kedelai secara In Vitro

Termite symbiotic bacteria have potential as a biological agent that has not been extensively researched. This research aims to uncover the potential of termite symbiotic bacteria as a biological agent against the damping off disease (Sclerotium rolfsii) in soybean plants. The research was conducted in the plant protection laboratory, biosciences laboratory, and innovation garden of Jember State Polytechnic over 4 months. The study was designed based on a Completely Randomized Design (CRD) with 6 treatments, each repeated 4 times. The treatments included: P1 pathogenic fungus as control, P2-P4 pathogen with termite symbiotic bacteria with the highest inhibition percentage, and P6 pathogen S. rolfsii. The stages of the research began with the Isolation of Termite Symbiotic Bacteria, Antagonistic Screening Test, Hypersensitivity Test, and In Vitro Antagonistic Test. From the results of testing four isolates, the ability to inhibit the pathogen S. rolfsii was observed. These isolates are IR1A1, IR1A4, IR1A6, and IR4D2. The effectiveness of inhibiting S. rolfsii ranged from 28.97% to 95.79% in vitro. The most effective bacterial isolate in suppressing the pathogen and capable of surpassing fungicide treatment is isolated IR1A6, which has an inhibition rate of up to 95.79%.

Antibiogram Profiles and Disinfectant Effectiveness on Bacterial Isolates in the Surgical Intensive Care Unit

Although studying bacterial contaminants in intensive care units has become a common topic, it is still being studied because of its importance in preserving the lives of patients and healthcare workers, where there is an increasing rate of the emergence of new bacterial strains that acquire resistance to antibacterial drugs. This is the first study targeting the surgical Intensive Care Unit (ICU) at Al-Wahda Hospital, Derna City, Libya. Standard traditional methods and molecular techniques were used to isolate, identify, and study the antibiogram profiles against bacterial isolates found to contaminate inanimate surfaces inside the ICU. The study results proved that the study area was contaminated with nine different bacterial genera belonging to Gram-positive (54%) and Gram-negative (46%) bacteria. The broadest antibiotic in this study was the highest inhibition percentage in this study was from Imipenem 10µg followed by Levofloxacin 5µg which suppressed the growth of 80% and 70% of all tested isolates, respectively. Among the tested disinfectants, hydrogen peroxide (Oxydol 3%) controlled (50%) of the tested bacteria, whereas glutaraldehyde (Prodex 99%) showed no activity. Povidone Hydro alcohol (Iodine 10%) actively controlled (70%) of the tested isolates. Conclusion: This study concludes that the targeted ICU is contaminated with nine different bacterial genera that show varied resistance to the tested antibiotics and disinfectants, threaten patients' and health workers' lives, and recommend the search for alternative effective antibiotics and disinfectants.

Evaluation of Some Plant Extract and Bio- Fungi to Control Leaf Spot of Safflower Plant Disease

This research study aims at evaluating the effectiveness of the biological fungus Trichoderma longibrachiatum , Trichoderma harzianum and some plant extracts kinocarpus( Leucaena leucocephala) ,Eucalyptus (Eucalyptus camaldulensis), henna leaf extract ( Lawsonia inermis ). Besides, finding the best concentrations of plant extracts to stimulate the systemic resistance to safflower plant Carthamus tinctorius, against alternaria leaf spot. The results made clear that the fungus Alternaria alternata had clear effect in reducing the percentage of germination of safflower seeds as the rate reached 53.33% while the percentage of seedling damping off reached 81.11% compared with control treatment 93.33, 0 % respectively. The biological fungi have high effectiveness with inhibiting growth of fungus Alternaria alternata, according to results as the antagonisim level was (1) as in bell scale. Plant extract also gave a high efficiency in preventing the infectious fungus's growth as rate of kinokarpus , henna, eucalyptus reached 75.78 ,74.59 , 70.64 % respectively. Effect of the extracts in inhibiting the pathogenic fungus went up with increased concentration, as the highest percentage of inhibition was reached at a concentration of 10 % for tested extracts, the kinocarpus plant gave the highest rate reached 91.51%, while the percentage reached 89.62, 80.36 % for henna and eucalyptus extract, respectively.

Development of Herbal Ultrasound Gel Prototype from Zingiber cassumunar Roxb., Curcuma longa L. and Zingiber officinale Roscoe for Antioxidant and Anti-Inflammation

This research aimed to determine the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of phlai, turmeric, ginger and combined extract of phlai, turmeric, and ginger at recipe 1:2:2 (PTG). In addition, the formula of herbal ultrasound gel for antioxidant and anti-inflammatory properties was developed. The TPC, flavonoid, antioxidant and anti-inflammation were investigated by using the Folin-Ciocalteu method, flavonoid, DPPH, ABTS and nitric oxide (NO) scavenging assay. The PTG extract was tested for the phytochemicals by GC-MS technique. The microbial contamination standard of herbal ultrasound gel prototype was determined. The result show that the PTG extract has the TPC and flavonoid at 105.11 ± 16.93 mg GAE/g extract and 63.58 ± 2.29 mg QE/g extract, respectively. Moreover, the DPPH and ABTS assay show that PTG extract has the half maximal inhibitory concentration (IC50) at 0.82 ± 0.11 and 0.98 ± 1.56 mg/mL, respectively. In addition, PTG extract has a high percentage of NO inhibition at 53.38 ± 0.16. Furthermore, the GC-MS method found that PTG extract has 7 active ingredients. The highest amount of phytochemical is AR-turmerone. The safety assessment for external use found that the herbal ultrasound gel prototype product passed standard criteria for microbial contamination at the laboratory level. To conclude, the PTG extract has a synergistic effect and a high ability to act as an antioxidant and anti-inflammation. Moreover, the PTG herbal ultrasound gel has a microbial contamination safety to use. Therefore, the prototype herbal ultrasound gel product may be further developed into health products to treat pain and inflammation efficiently in clinical practice.

Phytochemical Profiling by HPLC-ESI-MS/MS and InVitro Investigation of the Antidiabetic Activity of Cassia bakeriana Bark Extract and Fractions.

Type 2 diabetes mellitus is a global health problem, placing patients at a higher risk of developing cardiovascular diseases and cancer. This study investigates the antidiabetic potential of Cassia bakeriana bark extracts and fractions. We evaluate their ability to inhibit α-amylase and α-glucosidase enzymes and advanced glycation end-products (AGEs). The antioxidant potential was also examined. Extracts were prepared through maceration with hexane (HE) and ethanol (EE), and the fractions were obtained via liquid-liquid extraction from EE. Anti-enzymatic, anti-glycation, antioxidant, and cytotoxic assays were conducted in 96-well plates using different concentrations of samples to determine the half-maximal inhibitory concentration (IC50). Active samples were further analyzed using HPLC-(-)-ESI-MS/MS. The ethyl acetate fraction (EAF) demonstrated a high percentage of α-amylase inhibition (94.0%) with a promising IC50 value of 1.05 μg mL-1. Additionally, EAF displayed 61.5% inhibition of α-glucosidase, with an IC50 value of 537 μg mL-1. The EE, EAF, and n-butanol fraction (BF) exhibited strong anti-glycation capacities. Furthermore, the EE, EAF, BF, and dichloromethane fractions showed promising antioxidant activity using the DPPH and ORAC methodologies. Cytotoxic activity was also evaluated with Vero cells, revealing no adverse effects on cell viability (CC50 > 512.0 μg mL-1). Active samples predominantly comprised proanthocyanidins, flavonoids, and anthraquinone, representing the main constituents of C. bakeriana bark. This study provides the first assessment of the antidiabetic potential of C. bakeriana bark and a comprehensive analysis of the chemical composition of its active extracts and fractions, offering hope for future treatments.

Case-controlled study to compare the neutralizing antibody amongst fully vaccinated breakthrough infections in third wave of COVID-19 and asymptomatic healthcare workers

BackgroundThe SARS-CoV-2 (COVID-19) pandemic declared in March 2020 continues to impact millions globally. India initiated a large-scale COVID-19 vaccination campaign in January 2021 using the CovishieldTM vaccine. Monitoring neutralizing antibody (NAb) levels is essential for assessing vaccine effectiveness, but data on NAb levels after completing two doses of CovishieldTM are limited. This study aimed to investigate the NAb profile among fully vaccinated individuals. MethodsConducted during the third wave of the pandemic starting in January 2022, this study included individuals who received the first two doses of CovishieldTM and visited the flu clinic or vaccination center. NAb levels were measured using an approved kit and "Blocking ELISA" method. Symptomatic individuals underwent COVID-19 real-time polymerase chain reaction (RT-PCR) testing. ResultsThe study included 299 individuals with a median age of 26 years (IQR-11 years; male preponderance: 81.9%). NAb was detected in 91.5% (n=274) of the participants. The mean neutralizing percentage inhibition was 77.26%±26.26%. Significant relationships were found between neutralizing percentage inhibition and past COVID-19 infection (p=0.0005), blood groups (B+ve had the highest Ab inhibition, p=0.0003), and age (p=0.01). No significant correlation was observed with gender (p=0.11), time since the second vaccine dose (p=0.32), and time from the last COVID19 infection (p=0.48). ConclusionDuring the early stages of the third wave of COVID-19 in India (January 2022), a high seroprevalence of NAb was observed among fully vaccinated individuals. Breakthrough infections occurred but with reduced severity. Breakthrough cases had higher percentage inhibition, high NAb positivity, longer time since the last COVID-19 infection, and older age.

Synthesis and characterization of aluminum oxide nanoparticles (Al2O3‐NPs) and analysis of their antimicrobial efficacy against selected pathogenic microbes

AbstractThis present paper assesses the chemical synthesis of aluminum oxide nanoparticles (Al2O3‐NPs) using a modified sol–gel process. Aluminum nitrate and citric acid were heated to 180 °C until a gel was produced. After that, the dehydrated amorphous gel was sintered at 800 °C for 2 h. The formation of nano‐sized α‐Al2O3 particles was investigated using several characterized methodologies, encompassing Fourier transform infrared spectroscopy, X‐ray diffraction (XRD), dynamic light scattering, and X‐ray fluorescence (XRF). Then, the antibacterial and antifungal activity of synthesized nanoscale Al2O3 particles against pathogens like Escherichia coli, Staphylococcus aureus, and Trichophyton rubrum has been investigated. The successful synthesis of alumina nanoparticles has been validated by several analytical techniques. Nanoparticles of Al2O3‐NPs with a rhombohedral structure and an average crystallite size of 26 nm were revealed by XRD research. Their zeta potential was −8.65 mV, and their size distribution measured 68.6 nm in diameter. The elemental composition of sample was identified with the use of XRF, which indicates that the Al2O3‐NPs powder is quite pure. Furthermore, the antimicrobial experiment showed that the Al2O3‐NPs exhibited the greatest antibacterial efficacy, S. aureus, E. Coli, and T. rubrum exhibited significant inhibition zones with diameters of 13, and 15 mm, and a high inhibition percentage of 70% when exposed to 100 mg/mL of Al2O3 nanoparticles.

Apoptotic Effect of Simpor Leaf Extract (Dillenia suffruticosa) from Belitung on Colorectal Cancer Cells WiDr

Colorectal cancer is the second deadliest cancer worldwide. The side effects and resistance of existing chemotherapy drugs are the background for various studies looking for potential plant extracts as new anticancer agents. This study aims to determine the cytotoxic activity of simpor leaf extract from Belitung and its effect on apoptosis of WiDr colorectal cancer cells. The compound content in simpor leaf extract was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The cytotoxic activity of simpor leaf extract at different concentrations (25, 50 and 100 ppm) was determined by the MTT test and apoptosis induction by Annexin V/PI flow cytometry analysis. Based on the GC-MS chromatogram results, 13 different compounds were detected. Analysis of the relationship between extract concentration and the percentage of WiDr cell inhibition showed that an extract concentration of 100 ppm was the concentration with the highest percentage of inhibition (20.12 % ± 4.99) compared to concentrations of 50 ppm (13.95 % ± 2.32) and 25 ppm (15.90 % ± 1.38) with an inhibitory concentration value (IC50) of simpor leaf extract of 10.45 ppm. Induction of WiDr cell apoptosis by the extract was influenced by the concentration of simpor leaf extract, which was increase in the percentage of apoptotic cells from 16 % at a concentration of 25 ppm to 70.5 % at a concentration of 100 ppm. The results of this study show that simpor leaf extract from Belitung is able to induce apoptosis in WiDr colorectal cancer cells. HIGHLIGHTS Colorectal cancer treatment faces challenges such as drug resistance and side effects, leading to research on plant-based anticancer agents like simpor leaf extract from Belitung. Simpor leaf extract demonstrated cytotoxic effects on WiDr colorectal cancer cells, achieving an inhibition rate of 20.12 % at a concentration of 100 ppm and an IC50 value of 10.45 ppm. This study shows that simpor leaf extract can induce apoptosis in WiDr colorectal cancer cells, with the percentage of apoptotic cells increasing from 16 % at 25 ppm to 70.5 % at 100 ppm. GRAPHICAL ABSTRACT

Evaluation of the in vitro toxicity and anti-inflammatory activity of the methanolic extract of the leaves of Pistacia lentiscus L. harvested from northwestern Algeria.

Medicinal and aromatic plants have been used for thousands of years for their therapeutic properties, to treat various ailments and maintain health. This study was carried out to test the in vitro toxicity and anti-inflammatory activity of the methanolic extract of the leaves of Pistacia lentiscus L., native to the north-western region of Algeria. Toxicity was studied in vitro by the hemolysis test and anti-hemolytic activity on human red blood cells, while anti-inflammatory activity was assessed in vitro by the protein denaturation method. The study of toxicity by hemolysis of red blood cells showed a high rate of hemolysis at a dose of 200 mg/mL, with a hemolytic percentage of around 88.64%, while a high rate of anti-hemolysis was observed at a dose of 25 mg/mL, with an inhibition percentage of around 83.17%. Evaluation of anti-inflammatory activity revealed a high percentage of inhibition of ovalbumin denaturation, reaching 63.53% at the 2000 ug/mL dose, while the lowest percentage was obtained at the 3500 ug\mL concentration with 10.16%. This extract has a toxic substance but does not exceed the toxicity threshold which achieves significant anti-inflammatory activity with depandant doses.

Quercetin as a Modulator of PTPN22 Phosphomonoesterase Activity: A Biochemical and Computational Evaluation.

Cancer, a group of diseases characterized by uncontrollable cell proliferation and metastasis, remains a global health challenge. This study investigates quercetin, a natural compound found in many fruits and vegetables, for its potential to inhibit the phosphomonoesterase activity of protein tyrosine phosphatase nonreceptor type 22 (PTPN22), a key immune response regulator implicated in cancer and autoimmune diseases. We started by screening seven (7) natural compounds against the activities of PTPN22 in vitro. The initial screening identified quercetin with the highest percentage inhibition (81%) among the screened compounds when compared with ursolic acid that has 84%. After the identification of quercetin, we proceeded by investigating the effect of increasing concentrations of the compound on the activity of PTPN22. In vitro studies showed that quercetin inhibited PTPN22 with an IC50 of 29.59 μM, outperforming the reference standard ursolic acid, which had an IC50 of 37.19 μM. Kinetic studies indicated a non-competitive inhibition by quercetin with a Ki of 550 μM. In silico analysis supported these findings, showing quercetin's better binding affinity (ΔGbind -24.56 kcal/mol) compared to ursolic acid, attributed to its higher reactivity and electron interaction capabilities at PTPN22's binding pocket. Both quercetin and ursolic acid improved the structural stability of PTPN22 during simulations. These results suggest quercetin's potential as an anticancer agent, meriting further research. However, in vivo studies and clinical trials are necessary to fully assess its efficacy and safety, and to better understand its mechanisms of action.

Biological and Photocatalytic Activities of Silver Nanoparticles Synthesized from the Leaf Extract of Euphorbia royleana Boiss.

Silver nanoparticles (AgNPs) have gained significance due to their practical use in the medicinal field, especially in the treatment of tumors and cancer. The current article explores a green synthetic method for the preparation of AgNPs using leaf extract of Euphorbia royleanas. The synthesis was conducted at different parameters like concentration of AgNO3, pH, salt concentration, temperature and time to optimize best results for their biochemical applications. It was validated through UV-V spectroscopy (400-450 nm) with 1:3 (concentration ratio of leaf ethanolic extract and 1 mM AgNO3 solution) at a pH value of 8 at 35oC, which were the best optimization conditions. The FTIR spectral bands showed the presence of C-N and -OH functional groups, indicating that -OH stretching and the aliphatic -C-H stretching were involved in the reduction of Ag ions. The XRD pattern showed the face-centered cubic structure of silver nanoparticles. The results of SEM revealed that AgNPs were predominantly spherical in shape, mono-dispersed, and arranged in scattered form. EDX analysis testified the presence of metallic silver along with other elements like Cl, C, and O. The investigation of biochemical parameters showed that AgNPs were influential in the discoloration of dye wastewater (methylene blue ), where 80% of dye color was removed in 20 min, followed by the significant (p < 0.05) analgesic activity with an inhibition percentage of 86.45% at a dose of 500 mg/kg. Similarly, the antioxidant activity with the highest percent inhibition was 55.4% (p < 0.0001), shown by the AgNPs at 500 μg/mL. AgNPs showed a 30 mm zone of inhibition at 100 μl/mL against Aspergillus niger. It was concluded that AgNPs provide a baseline in medical technology for the treatment of simple to chronic diseases.

Prediction of Inhibitory Activity against the MATE1 Transporter via Combined Fingerprint- and Physics-Based Machine Learning Models.

Renal secretion plays an important role in excretion of drug from the kidney. Two major transporters known to be highly involved in renal secretion are MATE1/2 K and OCT2, the former of which is highly related to drug-drug interactions. Among published in silico models for MATE inhibitors, a previous model obtained a ROC-AUC value of 0.78 using high throughput percentage inhibition data [J. Med. Chem. 2013, 56(3), 781-795] which we aimed to improve upon here using a combined fingerprint and physics-based approach. To this end, we collected 225 publicly available compounds with pIC50 values against MATE1. Subsequently, on the one hand, we performed a physics-based approach using an Alpha-Fold protein structure, from which we obtained MM-GB/SA scores for those compounds. On the other hand, we built Random Forest (RF) and message passing neural network models using extended-connectivity fingerprints with radius 4 (ECFP4) and chemical structures as graphs, respectively, which also included MM-GB/SA scores as input variables. In a five-fold cross-validation with a separate test set, we found that the best predictivity for the hold-out test was observed in the RF model (including ECFP4 and MM-GB/SA data) with an ROC-AUC of 0.833 ± 0.036; while that of the MM-GB/SA regression model was 0.742. However, the MM-GB/SA model did not show a dependency of the performance on the particular chemical space being predicted. Additionally, via structural interaction fingerprint analysis, we identified interacting residues with inhibitor as identical for those with noninhibitors, including substrates, such as Gln49, Trp274, Tyr277, Tyr299, Ile303, and Tyr306. The similar binding modes are consistent with the observed similar IC50 value inhibitor when using different substrates experimentally, and practically, this can release the experimental scientists from bothering of selecting substrates for MATE1. Hence, we were able to build highly predictive classification models for MATE1 inhibitory activity with both ECFP4 and MM-GB/SA score as input features, which is fit-for-purpose for use in the drug discovery process.

Isolation of Potential Antagonistic Rhizosphere Fungi against Alternaria alternata from Organic Carrot Productions

Rhizospheric fungi can have serve as biological agents against plant pathogens Alternaria alternata, which infects carrot plants. Rhizospheric fungi are more abundant in organic lands. This study aims to isolate fungi from organic carrot’s rhizosphere that are able to suppress A. alternata’s growth and determine isolate rhizospheric fungi ability to inhibit A. alternata to identify potential biological control agents against A. alternata. This research was conducted at the Laboratory of Biotechnology and Basic Biology, Diponegoro University, between January–June 2023. Research methods include survey location, soil sampling, rhizospheric fungi isolation, pathogenic fungi isolation, creating growth curve, pathogenicity tests, antagonism tests, and fungi identification. This study used a complete randomized design (CRD) and was analyzed using ANOVA at the 5% level of significance and Duncan's hoc-posttest. Fungi isolation from organic carrot plant’s rhizosphere resulted in 13 isolates consisting of six genera: Penicillium, Aspergillus, Paecilomyces, Myrothecium, Trichoderma, and Simplicillium. The antagonism test showed that eight antagonistic isolates, including the genus Penicillium (WO1, WO6, WO10), Paecilomyces (WO5), Myrothecium (WO7), Trichoderma (WO9), and Aspergillus (WO11, WO13), where WO1, WO6, WO10, and WO11 have a medium percentage of inhibition (44.12-57.84%), while WO5, WO7, WO9, and WO13 have a high percentage of inhibition (64.29-76.06%). The eight isolates showed antagonism mechanisms, including antibiosis (WO1, WO5, WO6, WO7, WO10, and WO11) and parasitism (WO9 and WO13).

Potential mechanism of gallic acid-coated iron oxide nanoparticles against associated genes of Klebsiella pneumoniae capsule, antibacterial and antibiofilm.

Antibiotic resistance has increased in recent years, especially for pathogens like Klebsiella pneumoniae. Discovering and developing new drugs is challenging due to the high resistance of pathogens. Metal nanoparticles have been widely used in recent years to overcome and treat infections. Gallic acid-coated iron oxide nanoparticles (IONPs-GA) were synthesized in a simple and cost-effective method. The morphology characteristics of synthesized IONPs-GA were analyzed using Fourier transform infrared spectroscopy (FTIR), x-ray diffraction analysis (XRD), and scanning electron microscope (SEM) analysis. IONPs were mostly spherical in shape with sizes ranging between 32 and 61 nm. All analyses used in this study confirmed the successful coating of gallic acid to iron oxide. Biological activities were studied phenotypically and on the molecular level, including antibacterial, antibiofilm, and mRNA levels of capsule-associated genes. The results showed high antimicrobial activity of the synthesized nanoparticles against different G+ve and G-ve bacteria. The highest activity was recorded against Staphylococcus aureus (43 mm) and K. pneumoniae (22 mm). The MIC of IONPs against K. pneumoniae was 3.12 mg/mL and SEM analysis showed adhering the IONPs-GA to the cell surface of K. pneumoniae resulted in disrupting the cell membrane. Different concentrations of sub-MIC inhibited K. pneumoniae biofilm formation with the highest inhibition percentage at ½ × MIC (66.86%). Also, the synthesized IONPs-GA differently affected the regulation and mRNA level of capsule-associated genes in K. pneumoniae. The results indicated that IONPs-GA could be useful in biological applications such as in drug delivery and treatment wide range of pathogens. RESEARCH HIGHLIGHTS: Gallic acid was successfully coated into iron oxide nanoparticles synthesized in a simple way. IONPs-GA was morphologically characterized using FTIR, XRD, and SEM. Evaluation the activity of IONPs-GA as antibacterial, antibiofilm, and study the potential level of mRNA affected by IONPs-GA.

GC-MS Analysis, Antioxidant, Antidiabetic Activity, and ADMET Study of Diospyros mespiliformis Hochst. Ex A. DC. Ebenaceae Stembark

The present study carried out GC-MS analysis, antioxidant, antidiabetic, and ADMET study of the crude ethanol extract (CRE), ethyl acetate (EAF), and aqueous (AQF) fractions of Diospyros mespiliformis (DM). Grandiflorenic (25.36%) and cis, cis-linoleic (27.13%) acids were the most abundant of the 59 and 40 compounds identified in the EAF and AQF, respectively. The EAF and CRE exhibited total antioxidant capacity of 77.78 ±0.01 ascorbic acid equivalent (mg AAE/g extract) and 60.20 ±0.01 mg AAE/g extract, respectively, significantly (p &lt; 0.05) than the AQF (29.63 ±0.01 mg AAE/g extract). Furthermore, the EAF (38.27% ±2.44) exhibited a significantly (p &lt; 0.05) higher percentage inhibition via the ferric thiocyanate assay than the CRE (17.45% ±2.18) and AQF (15.91% ±2.11). All the extracts showed significantly (p &lt; 0.05) lower malondialdehyde concentrations than AA in the thiobarbituric acid assay. Additionally, the AQF exhibited a significantly higher percentage of H2O2 scavenging than the AA and EAF at 100 µg/ml. Diazoprogesterone (compound III) identified in the EAF exhibited the lowest respective binding affinity and inhibition constant, interacting with myeloperoxidase (-9 kcal/mol and 0.25 µM), xanthine oxidase (-9.9 kcal/mol and 0.05 µM), 11-β-hydroxysteroid dehydrogenase (-10 kcal/mol and 0.05µM), and sirtuin 6 (-10 kcal/mol and 0.05 µM). The molecular dynamics simulations showed residue fluctuations of the diazoprogesterone-docked complexes with the highest observed at Ser42 (5.14 Å), Cys1325 (8.96 Å), Ser281 (7.71 Å), and Leu78 (3.91 Å) for myeloperoxidase, xanthine oxidase, 11-β-hydroxysteroid dehydrogenase, and sirtuin 6, respectively compared to the undocked targets. Moreover, diazoprogesterone was predicted to possess good ADMET properties. Conclusively, DM possesses significant antioxidant and antidiabetic potential, containing compounds that might be a source of novel therapeutics against oxidative stress and diabetes.

Evaluation of butterfly pea powder addition to cow’s milk kefir on the antibacterial activity against bacteria causing foodborne disease

Abstract Foodborne diseases are caused by consuming food contaminated with pathogens such as bacteria, viruses, parasites, or toxic chemicals, as well as biotoxins. Bacteria responsible for foodborne disease include Escherichia coli and Staphylococcus aureus. The typical approach to managing foodborne disease involves the use of antibiotics, which might lead to resistance over time. Kefir is a probiotic drink that can help maintain health by balancing the natural gut microflora. This study aims to investigate the impact of adding butterfly pea powder to kefir on its antibacterial activity against the bacteria that cause foodborne disease. Kefir was prepared by adding butterfly pea powder in six different concentrations (0%, 0.5%, 1%, 1.5%, 2%, and 2.5%) with four replications. Kefir’s pH, total titrated acid, total LAB, and its antibacterial activity using the microdilution method were observed. The results revealed that the addition of butterfly pea flower powder at various concentrations significantly influenced the pH value, total acidity, and antibacterial effects against E.coli and S.aureus but did not significantly affect the total LAB count. Butterfly pea flower powder reduced the pH value, increased the total acidity, and enhanced the antibacterial activity, resulting in a higher percentage of inhibition against the tested bacteria without adversely affecting LAB growth. It was concluded that adding butterfly pea powder to cow milk kefir potentially improves the antibacterial functions against bacteria causing foodborne disease.

Efeito da adubação orgânica na produção de ácidos graxos e atividade antidiabética de Portulaca oleracea L.

Portulaca oleracea L., commonly known as purslane, is a plant consumed in Europe, Asia and Africa, with nutraceutical compounds and essential fatty acids whose content exceeds that of vegetables such as spinach and lettuce. On the other hand, organic fertilization improves the physical-chemical characteristics of the soil and thus promotes growth, physiology and secondary metabolism, improving the chemical composition of the plant. In order to evaluate the effect of organic fertilization on fatty acid content and anti-diabetic activity (α-Glucosidase), greenhouse-grown plants were subjected to three treatments: control without additional fertilizers, with compost and vermicompost. The results indicated that vermicompost significantly increased oleic and linoleic acid content. However, palmitic acid content decreased, although other saturated fatty acids such as lauric, myristic and stearic acids did not show variations in the different treatments. Of the polyunsaturated essential fatty acids, linolenic acid (omega-3) increased in the control treatment. On the other hand, the enzyme α-Glucosidase did not show differences in the different fertilization treatments. However, its high percentage of inhibition (60 %) positions it as a species with high antidiabetic potential.