High Organic Loading Research Articles

Culex quinquefasciatus larvae development arrested when fed on Neochloris aquatica.

Culex quinquefasciatus is a cosmopolitan species widely distributed in the tropical and subtropical areas of the world. Due to its long history of close association with humans, the transmission of arboviruses and parasites have an important role in veterinary and public health. Adult females feed mainly on birds although they can also feed on humans and other mammals. On the other hand, larvae are able to feed on a great diversity of microorganisms, including microalgae, present in natural or artificial breeding sites with a high organic load. These two particularities, mentioned above, are some of the reasons why this mosquito is so successful in the environment. In this work, we report the identification of a microalga found during field sampling in artificial breeding sites, in a group of discarded tires with accumulated rainwater. Surprisingly, only one of them had a bright green culture without mosquito larvae while the other surrounding tires contained a large number of mosquito larvae. We isolated and identified this microorganism as Neochloris aquatica, and it was evaluated as a potential biological control agent against Cx. quinquefasciatus. The oviposition site preference in the presence of the alga by gravid females, and the effects on larval development were analyzed. Additionally, microalga effect on Cx. quinquefasciatus wild type, naturally infected with the endosymbiotic bacterium Wolbachia (w+) and Wolbachia free (w−) laboratory lines was explored. According to our results, even though it is chosen by gravid females to lay their eggs, the microalga had a negative effect on the development of larvae from both populations. Additionally, when the larvae were fed with a culture of alga supplemented with balanced fish food used as control diet, they were not able to reverse its effect, and were unable to complete development until adulthood. Here, N. aquatica is described as a biological agent, and as a potential source of bioactive compounds for the control of mosquito populations important in veterinary and human health.

Electroflotation enables treatment of effluents generated during pyrolytic biomass revalorization

Wastewater produced during biomass revalorization contains high organic load. Organic compounds generated during pyrolysis of biomass are hardly removed by conventional treatments. If sustainable treatment of these effluents can be enforced, biomass revalorization would be an excellent sustainable approach for energy production. This work explores the competitiveness of electroflotation as emerging electrochemically-driven separation technology using a scaled-up continuous-flow electroflotation reactor (CFER). Electrogeneration of gas bubbles drives the separation of hydrophobic organic compounds from aqueous solution. Experimental designed was employed to optimize the operational variables (i.e., applied current density and sodium chloride concentration) at different flow rates. Under conditions of 3.5 mA cm−2, 2.0 g L-1 of NaCl, and flowrate of 100 L h−1, complete decolorization of effluent was obtained with a total organic abatement of 55%. Chromatographic analyses demonstrated complete removal of phenolic compounds. These results identify electroflotation as suitable and promising pre-treatment to decrease the high-organic loads of pyrolytic wastewater.

Possibility of using waste edible oil for biogas production

Anaerobic digestion is a process by which energy from organically degradable waste can be recovered in the form of biogas. The uncontrolled disposal of such organic waste is very burdensome to the environment. In Slovenia, large quantities of waste edible oils are produced every year, which could be used for biogas production. In this study, we investigated the methane potential of waste edible oil in a batch reactor. Further in the pilot scale in a semi-continuous experiment we evaluated the use of waste edible oil in anaerobic digesters of wastewater treatment plant. The results show that the quantity of oil per day that is fed into the anaerobic reactor is limited due to the process's instability and lower biogas production. The optimal daily organic loading of waste edible oil was between 1.6 and 2.4 g VS/L inoculum. During these conditions, the process of anaerobic digestion was stable and methane production was highest. At higher organic loading, the process became unstable, pH was reduced, volatile fatty acids increased, COD at the outflow of reactor increased, and biogas production was significantly reduced.

Mild chemical pretreatment of cassava pulp for enhancing high-load anaerobic digestion

Lignocellulosic components limit the potential of starch-rich cassava pulp for high-load anaerobic digestion. Mild chemical pretreatments using 0.1%–3.0% (w/v) of H2SO4, HCl, NaOH, and KOH were investigated at low temperature (30 °C) for enhancing organic solubilization and methane production. Alkalis solubilized up to 54% of the chemical oxygen demand, degraded 53% of hemicellulose, and thus increased 18–33% of methane yield. The maximum methane yield was 324 ± 5 mL/gVS achieved by 2.0% NaOH pretreatment. Modified fed-batch BMP constructively elucidated the enhancing and hindering effects of pretreatment on methane production at high organic loading. Suitable mild pretreatment is essential for developing a practical digestion process at higher organic loading rates.

Rapid Assessment Method for Evaluation of the Weighted Contribution of Anthropogenic Pollution: A Case Study of Lake Burullus, Egypt

This paper proposes a pragmatic approach for rapid assessment of the weighted contribution of the main waste streams contributing to pollution of surface water bodies. A case study was conducted on Lake Burullus in Egypt. The lake suffers from pollution due to many human-based activities around the lake, such as domestic, industrial, agriculture, fish farming, and solid wastes. The weighted contribution of these activities was assessed in terms of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP). The results showed that the highest organic load is due to the domestic wastewater pollution component (63.2% of COD load), followed by fish aquaculture (35.4%). The highest TN (43.9%) and TP (58.3%) pollutant loads to Lake Burullus are due to the agricultural pollution component, followed by fish aquaculture with pollutant loads of 28.5% and 25.3%, respectively. The industrial wastewater pollution component has a very small effect on the pollution of Lake Burullus. The assessment of this study will help identify and magnify the key polluters and thus guide the decision-makers to prioritize investment planning for depollution intervention projects. For example, if the target is to reduce COD loads, investments must be directed toward the rehabilitation and expansion of wastewater treatment plants (WWTPs).

Impacts of granular activated carbon addition on anaerobic granulation in blackwater treatment

Upflow anaerobic sludge blanket (UASB) reactors, with or without granular activated carbon (GAC) amendment, were applied for blackwater treatment. The impact of GAC on the formation of granules and biomethane recovery was assessed. High organic loading rates (OLRs) up to 15.7 ± 2.1 kg COD/(m3d) were achieved with both reactors. Similar chemical oxygen demand (COD) removal and methane production rate were observed with OLRs ranging from 5.1 ± 1.0 to 9.3 ± 1.5 kg COD/(m3d). Under higher OLR conditions (13.6 ± 1.1 to 15.7 ± 2.1 kg COD/(m3d)), the GAC-amended UASB achieved a higher COD reduction than the UASB without GAC addition. Interestingly, volatile suspended solids (VSS) concentrations, granule size, and extracellular polymeric substance concentrations were lower in the GAC-amended UASB reactor as compared to the UASB without GAC. The methanogenesis activity of the granules in the GAC-amended UASB reactor was significantly higher than the methanogenesis activity of the UASB granules. The microbes o_Bacteroidales and Syntrophus were predominant in both reactors. The acetoclastic methanogens dominated in the UASB reactor without GAC addition; while hydrogenotrophic methanogens dominated in the GAC-UASB reactor. A phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) indicated that syntrophic acetate oxidation improved with GAC addition. The co-occurrence network indicated that interactions between dominant bacteria and archaea were higher in the GAC-amended UASB reactor than in the UASB reactor without GAC addition. This study demonstrated the improved blackwater treatment performance as a result of granulation in UASB with the addition of GAC.

Opportunities for Waste to Energy in the Milk Production Industry: Perspectives for the Circular Economy

Cheese whey is a waste produced in the dairy industry which generates problems if it is dumped directly into the sewer due to its high organic load. An alternative for cheese whey management is anaerobic digestion, a biological process that transforms organic matter into biogas and digestate, two products with significant energy and agricultural potential. This work was aimed at contributing to the building of knowledge about the anaerobic degradation of cheese whey, developing a bibliometric analysis, and tracing trends in related research from 2010 up to the present, using PRISMA® to develop a systematic review based on Scopus® and using Excel® and bibliometric software (VosViewer® and RefViz®) for the identification of information. Our results show that the research around cheese whey is relatively recent and that the highest percentage of publications is from 2018 onwards. Twelve variables of the anaerobic cheese whey degradation process were identified and grouped into five factors: substrate, reactor configuration, digestate analysis, microbiological analysis, and inoculum. Likewise, it was identified that most of the anaerobic processes allow the implementation of the circular economy into the dairy sector. In conclusion, the application of anaerobic digestion in the dairy sector can help to close the productive cycles, produce biofuels, and reduce pollution.

Ecological insight into incompatibility between polymer storage and floc settling in polyhydroxyalkanoate producer selection using complex carbon sources

Polyhydroxyalkanoate (PHA) producer selection is a key step in mixed culture (MC) production. This study focused on incompatibility between PHA storage and floc settling of MCs in the selection process. In a selector using fermented waste activated sludge as substrate under varying organic loading, average maximum PHA content obtained in batch assays increased by ∼ 22 wt% and biomass concentration increased by ∼ 34% with the increasing of organic loading. However, poor floc settling occurred, causing decreased batch PHA production and costly downstream process. A flank community which can corporately use non-VFA organics existed in the selector. When organic loading increased, PHA producers had stronger negative interactions, but not cooperation with the flank community members. Thus, high PHA storage of MCs was bounded to the domination of core PHA producer. But the domination of Thauera bacteria under high organic loading indirectly induced a bloom of filamentous bacteria.

Dynamics of phytoplankton community in seasonally open and closed wetlands in the Teesta-Torsa basin, India, and management implications for sustainable utilization.

The present study deals with the broader understanding of phytoplankton assemblage pattern and their ecohydrological interactions in two ecologically distinct floodplain wetlands of Teesta - Torsa basin, India. Analyses of data revealed significant seasonal variations (p ≤ 0.05) of ten water variables (temperature, transparency, pH, conductivity, total dissolved solids, dissolved oxygen, total hardness, total alkalinity, PO4 - P, and SiO4 - Si) in both the wetlands; however, no significant variation was observed among the sampling stations. In total, 128 species of phytoplankton were recorded (118 species belonging to 94 genera in seasonally open; 103 species belonging to 86 genera in closed wetland). Four algal groups, viz. Cyanophyceae, Coscinodiscophyceae, Bacillariophyceae, and Chlorophyceae, were the dominant quantitative component, remarkably influencing the total phytoplankton population in both the wetlands, contributing ~ 87% of total phytoplankton. Species Aulacoseira granulata alone contributed 12 - 41% and 8 - 34% to the total phytoplankton in the seasonally open and closed wetland, respectively, and indicated high organic load in both the wetlands. Altogether thirty-six and thirty-one phytoplankton taxa appeared as major indicators across the seasons for seasonally open and closed wetland, respectively. The indicator taxa (Aulacoseira, Oscillatoria, Dolichospermum, Spirogyra, Synedra, Nitzschia, Navicula, Euglena, Phacus) in both the wetlands hinted that the wetlands are under pollution pressure. The assemblage structure of phytoplankton was related to transparency, NO3 - N, PO4 - P, SiO4 - Si, total dissolved solids, and temperature as evident from BIO - ENV. Furthermore, the marginal test also selected similar variables (depth, transparency, conductivity, PO4 - P, SiO4 - Si) for seasonally open and the variables such as depth, conductivity, total dissolved solids, total alkalinity, and NO3 - N for the closed wetland. The study showed that the seasonal riverine connectivity greatly influences the variations in phytoplankton community in the seasonally open wetland.

Olive mill wastewater phytoremediation employing economically important woody plants

In this study two plant species, Punica granatum L. and Myrtus communis L., have been tested as candidates for phytoremediation of olive mill wastewater (OMW) through recirculation in soil pilot units, according to the proposed patented technology by Santori and Cicalini [EP1216963 A. 26 Jun 2002]. Wastewater was treated in batches of low to high organics strength (COD: 2 700–45 700 mg/L) during summer months of two consecutive years. Dynamics of the most important wastewater parameters were investigated, and corresponding removal rates were estimated. During treatment of low organic load OMW, average removal rate of organics, phenolics, total nitrogen and total phosphorus were 0.68 g-COD/kg-soil d, 0.073 g-TPh/kg-soil d, 0.033 g-TN/kg-soil d and 0.0074 g-TP/kg-soil d respectively and plants proved to be tolerant to the OMW. During treatment of high organic load OMW removal rates were roughly 10-fold higher although phytotoxic symptoms were observed. Plants were found to contribute greatly to the OMW treatment process since organics removal rates in pilot units were found to be at least 10-fold higher than in wastewater treatment in non-vegetated soil.Plant species with high added value products such as pomegranate and myrtle trees were used in this study, improving the circular economy potential of the aforementioned technology. Moreover, its efficiency has been demonstrated by quantification of the overall removal rates of key constituents as well as the contribution of the plants in the OMW treatment.

Recycled paper mill process water pre-treatment using ultrafiltration for water system closure

The use of membranes in integrated water systems has garnered increasing attention in paper mills operating with virgin fibres, with documented closed water loops in industry. However, limited research exists on the performance of ultrafiltration (UF) in mills operating with 100% recycled fibres (RCF) and closed water systems, as these result in the introduction and accumulation of insoluble contaminants in the effluent. This study investigated the effect of transmembrane pressure (TMP) and feed organic load on the flux, types of fouling, and organic separation with UF membranes in a recycled paper mill using 100% RCF. The high feed organic load caused inevitable fouling, limiting the highest achievable flux. However, the TMP could be manipulated to improve on or exacerbate the process performance, within the range that was set as a result of the feed's characteristics. Low TMP (1.35 bar) generated the highest permeability and chemical oxygen demand removal, while higher TMPs produced higher fluxes and recoveries. The process was characterised by high initial fluxes, followed by cake formation, which dominated, and pore blockage. The excess cake formation induced cake filtration. This study shows the potential of UF membranes to be used as pre-treatment in filtering the process water in a recycled paper mill operating with 100% RCF, a critical step forward in implementing an integrated water system, and eventually closing the water loop.

Treatment capacity of a novel flexible fibre biofilm bioreactor treating high-strength milk processing wastewater

ABSTRACT This study was focused on the capacity investigation of a novel multistage flexible fibre biofilm reactor (MS-FFBR) to treat milk processing wastewater (MPW) with high organic loading (OLR). The MS-FFBR performance was evaluated at four intermediate stages separately, and also the final effluent quality of the overall system with an influent chemical oxygen demand (CODin) ranged from 1500 ± 20 to 6000 ± 50 mg/L and hydraulic retention times (HRTs) of 8, 12, and 16 h. By comparting the bioreactors into the four stages effectively enhanced the bioreactor’s performance. The maximum TCOD removal efficiency was achieved at the first stage, which was about 89 ± 20, 82 ± 20, and 78 ± 20% at HRTs of 16, 12, 8 h, and low CODin of 1600 ± 20, 1590 ± 20, and 1673 ± 20 mg/L, respectively. However, the first stage had less contribution to TCOD removal at high CODin concentrations, reported to be about 42 ± 4%, 46 ± 4%, and 25 ± 4% at CODin of 5960 ± 40, 5830 ± 40, and 5870 ± 40 mg/L, respectively. Furthermore, the MS-FFBR was effective in removing total suspended solids (TSS) and turbidity. The bioreactor has reduced the effluent turbidity to 9.0 ± 0.2, 20.0 ± 0.6, and 16.1 ± 0.5 NTU at low CODin concentrations of 1600 ± 20, 1590 ± 20, and 1670 ± 20 mg/L and HRTs of 16, 12, and 8 h, respectively. The bioreactor revealed a high COD removal rate increased from 2.3 ± 0.1 to 12.2 ± 0.4 kg TCOD/m3d by increasing the OLR from 2.4 ± 0.1 to 17.6 ± 0.4 kg TCOD/m3d, confirming high reactor capacity for treatment of high-strength wastewater. Kinetic studies confirmed that the biomass yield was low at various HRTs ranging from 0.1 to 0.2 gVSS/gCOD.

Process Simulation and Optimization of Anaerobic Co-Digestion

Anaerobic digestion (AD) is an established method that has been extensively utilized for waste management, waste treatment, and biogas production. Anaerobic co-digestion (AcoD) is regarded as a practical approach to address substrate characteristics and system optimization issues. The distinction between AcoD and mono-digestion is that AcoD has a higher organic loading and significant substrate composition variation. There are many factors involved in AcoD which affect the biogas plant’s production ability and performance. Using response surface methodology (RSM) to forecast the optimal conditions for maximum biogas output, this article provides an overview of the different operational parameters in the AcoD process, modeling of the AcoD process, and overall process optimization. Standard software used for AcoD process simulation are Aspen Plus, SuperPro Designer, BioWin, CFD, and MATLAB. Review addresses design, development, and optimization frameworks for biogas production systems that take numerous aspects into account. The most significant AcoD optimization parameters include temperature, co-substrate concentration, inoculum ratio (percent), and C/N ratio.

Changes in rearing water microbiomes in RAS induced by membrane filtration alters the hindgut microbiomes of Atlantic salmon (Salmo salar) parr

An important step in water treatment in recirculating aquaculture systems (RAS) is the removal of particulate organic matter. While commonly applied treatment technologies remove most of the particles, fine particulate organic matter typically accumulate in RAS, and serve as substrate for heterotrophic bacteria in the system. Membrane ultrafiltration has been shown to reduce the concentrations of total organic carbon and to counteract blooms of opportunistic bacteria in periods with high organic loading. The potential influence of such opportunistic bacteria on the fish microbiomes and fish welfare is poorly understood. In this study, we investigated the effects of high organic loading on the water and hindgut microbiomes in RAS with Atlantic salmon parr. Two pilot-scale RAS, one with membrane ultrafiltration of a 10–15% side-stream (mRAS), and one without (cRAS), were operated in periods with low and high organic loadings, and the water and hindgut microbiomes were characterized by Illumina sequencing of 16S rDNA amplicons. At the end of the experiment, after a period of high organic loading, two OTUs representing Mycobacterium and Spartobacteria dominated the water microbiomes in cRAS, with relative abundances as high as 24 and 17%, respectively. In the mRAS water they were rare (relative abundances of 1.9 and 0.1%). The membrane ultrafiltration stabilized the water microbiota and efficiently prevented the growth of these bacterial populations in RAS. The parr hindgut microbiomes were generally highly distinct from the RAS water microbiomes and were dominated by the bacterial phyla Bacilli and Firmicutes. Furthermore, an OTU probably representing Carnobacterium inhibens, originally isolated from the gut of adult salmon, was highly abundant in the gut microbiomes, with relative abundances reaching over 30%. However, for the cRAS fish after a period with high organic loading, the relative abundance of this Carnobacterium OTU was reduced, and an increased similarity between the gut and the water microbiomes was observed. The Mycobacterium and Spartobacteria OTUs that were abundant in the cRAS water were also abundant in the cRAS hindgut samples. This indicates a direct influence of the water microbiomes on the gut microbiomes, and that high organic loading in the cRAS resulted in growth of opportunistic bacteria that were able to colonize the fish gut. The present study shows that membrane filtration is an applicable strategy to counteract growth of opportunistic bacteria in RAS and to promote positive fish – microbe interactions.

Development of a high-rate anaerobic thermophilic upflow packed bed reactor for efficient bioconversion of diluted three-phase olive mill wastewater into methane

Olive mill wastewater is the main by-product derived from olive mills using the three-phase extraction process, displaying a serious environmental risk due to its notable content in organics and phenolics. This work focused on a high-rate thermophilic anaerobic digester development, able to remove high organic load amounts, by converting it to biogas. The proposed system is an upflow packed bed reactor with recycling stream and biomass carriers appropriate for inoculum colonization. An anaerobic inoculum was acclimatized under thermophilic conditions and subsequently added into the reactor. Its performance examination against twice-centrifuged olive mill wastewater of 40 g COD/L followed, testing several runs of gradually decreased hydraulic retention time, reaching steady-state conditions until the minimum of 4.2 days, while optimum performance was achieved at 5.6 days. The production of 9.51 ± 0.37 NLCH4/LFeed is considered quite promising for the high-rate anaerobic treatment plants development for olive mill wastewater valorization, without the need of co-digestion.

Water Quality Assessment and Potential Source Contribution Using Multivariate Statistical Techniques in Jinwi River Watershed, South Korea

To investigate the effects of rapid urbanization on water pollution, the water quality, daily unit area pollutant load, water quality score, and real-time water quality index for the Jinwi River watershed were assessed. The contribution of known pollution sources was identified using multivariate statistical analysis and absolute principal component score-multiple linear regression. The water quality data were collected during the dry and wet seasons to compare the pollution characteristics with varying precipitation levels and flow rates. The highest level of urbanization is present in the upstream areas of the Hwangguji and Osan Streams. Most of the water quality parameter values were the highest in the downstream areas after the polluted rivers merged. The results showed a dilution effect with a lower pollution level in the wet season. Conversely, the daily unit area pollutant load was higher in the rainy season, indicating that the pollutants increased as the flow rate increased. A cluster analysis identified that the downstream water quality parameters are quite different from the upstream values. Upstream is an urban area with relatively high organic matter and nutrient loads. The upstream sewage treatment facilities were the main pollution sources. This study provides basic data for policymakers in urban water quality management.

Calcium hypochlorite pretreatment improves thermophilic digestion of waste activated sludge in an upflow anaerobic sludge blanket reactor

Anaerobic wasted activated sludge (WAS) digestion has been widely applied to reduce sludge volume and generate bioenergy in the form of methane. However, anaerobic WAS digestion performance is often challenged with poor hydrolysis of biomass cellular structures. In the present study, the feasibility of using calcium hypochlorite (Ca(ClO)2) to improve the thermophilic digestion of WAS was studied. Two thermophilic upflow anaerobic sludge bed (UASB) reactors (one with and one without Ca(ClO)2 pretreatment) were operated for 120 days under low and high organic loading rate (OLR) conditions, corresponding hydraulic retention time (HRT) of 10 days and 6 days, respectively. Both reactors achieved satisfied performance during the studied period. Under the low OLR condition, Ca(ClO)2 pretreatment significantly improved WAS total volatile solids (VS) removal efficiency (from 48.06 ± 2.63% to 57.34 ± 3.54%) and methane yield (from 289.2 ± 27.6 to 362.2 ± 36.7 N mL/g VS). However, no significant improvement was observed under the high OLR condition. g_S1 and g_Fervidobacterium were predominant bacteria in the thermophilic UASB reactor fed with Ca(ClO)2 pretreated WAS. Methanosarcina was dominant archaea in both reactors. The treatment mechanism and application potential of using Ca(ClO)2 to enhance the WAS digestibility were further discussed.

Inactivation of human norovirus by chlorous acid water, a novel chlorine-based disinfectant

IntroductionHuman norovirus (HuNoV) is a leading cause of infectious gastroenteritis. Since HuNoV shows resistance to alcohol, chlorine-based sanitizers are applied to decontaminate the virus on environmental surfaces. Chlorous acid water (CA) has been recently approved as a novel chlorine-based disinfectant categorized as a Type 2 OTC medicine in Japan. In this study, we aimed to evaluate the capability of CA to inactivate HuNoV. MethodsHuNoV (genogroups GII.2 and GII.4) was exposed to the test disinfectants including CA and sodium hypochlorite (NaClO), and the residual RNA copy was measured by reverse transcription quantitative PCR (RT-qPCR) after pretreatment with RNase. In addition, the log10 reduction of HuNoV RNA copy number by CA and NaClO was compared in the presence of bovine serum albumin (BSA), sheep red blood cells (SRBC), polypeptone, meat extract or amino acids to evaluate the stability of these disinfectants under organic-matter-rich conditions. ResultsIn the absence of organic substances, CA with 200 ppm free available chlorine provided >3.0 log10 reduction in the HuNoV RNA copy number within 5 min. Even under high organic matter load (0.3% each of BSA and SRBC or 0.5% polypeptone), 200 ppm CA achieved >3.0 log10 reduction in HuNoV RNA copy number while less than 1.0 log10 reduction was observed with 1,000 ppm sodium hypochlorite (NaClO) in the presence of 0.5% polypeptone. CA reacted with only cysteine, histidine and glutathione while NaClO reacted with all of the amino acids tested. ConclusionsCA is an effective disinfectant to inactivate HuNoV under organic-matter-rich conditions.

Distinct granulation pathways of aerobic granular sludge under poly aluminum chloride enhancement

Aerobic granular sludge (AGS), a novel strategy for nutrient removal which exhibits compact structure, good settleability, and resilience against high organic load, has been considered as a highly potential wastewater treatment technology. However, the long start-up period for granulation prevented its widespread development. In this study, the distinct pathways of PAC-enhanced AGS granulation were systematically investigated. Four identical sequencing batch reactors (SBR) with different PAC dosages (with 0, 50, 100, 400 mg/L effective Al3+ respectively) were applied. It was observed that the presence of PAC accelerated granules formation, promoted mechanical strength as well as denitrification rate of granules, and thus notably enhanced removal efficacies of COD, NH4+-N, NO2− and NO3−. According to the dissolved oxygen (DO) distribution inside the sludge and the denitrification rate (SDNR) measurements, distinguishing structures of granules under different PAC addition were discovered. Comparatively, AGS under low PAC addition (i.e., 50 mg/L) resulted in the largest granule size, the biggest anaerobic zone and the highest denitrification rate. Presumably, for the system with the low PAC addition (50 mg/L), appropriate aluminum ions (Al3+) neutralized part of the negative charge on the microorganism surface, thereby promoting cells aggregation. In contrast, a high dosage of PAC (400 mg/L) induced excessive Al3+ absorbed on the cell surface after neutralization, which increased the repulsive force between microorganisms, leading to more cavities and channels existed inside the granules. Therefore, granules under low PAC dosage (i.e., 50 mg/L) presented large anaerobic zone and high denitrification rate, thus favored the best internal structure and nutrients removal efficiencies.

Evaluation of electrocoagulation in the post-treatment of anaerobic wastewater of slaughterhouse and packing plant

The demand for food production brings with it the increase of effluent generation, which represents a great problem for slaughterhouses, since effluents contain higher organic load, requiring adequate treatment to decrease environmental impacts. This study examines an alternative to solve this problem: the application of electrocoagulation in the treatment of anaerobic slaughterhouse and packing plant effluents, as this promising technique is both compact and robust. The removal of color, COD and turbidity was analyzed, and the operational cost was calculated. To optimize the process, the CCRD (central composite rotatable design) methodology was used with two independent variables: electric current density and electrolysis time, obtaining a complete factorial of 2² with 4 axial points and 4 repetitions at the central point. The most expressive removals were: 87%, 80% and 76% for color, COD and turbidity, respectively, the lower operational cost obtained was 0.12 US$.m-3. The statistical analysis allowed obtaining valid mathematical models for color removal and cost and, through the analysis of desirability, it was found that for current density of 8 mA.cm-2 and time of 20 minutes it is possible to maximize color removal (84%) and minimize the cost (0.21 US$.m-3).
 Keywords: color, cost, COD, turbidity.