High-frequency Ultrasound Elastography Research Articles

Estimating the viscoelastic anisotropy of human skin through high-frequency ultrasound elastography.

The skin is the largest organ of the human body and serves distinct functions in protecting the body. The viscoelastic properties of the skin play a key role in supporting the skin-healing process, also it may be changed due to some skin diseases. In this study, high-frequency ultrasound (HFUS) elastography based on a Lamb wave model was used to noninvasively assess the viscoelastic anisotropy of human skin. Elastic waves were generated through an external vibrator, and the wave propagation velocity was measured through 40MHz ultrafast HFUS imaging. Through the use of a thin-layer gelatin phantom, HFUS elastography was verified to produce highly accurate estimates of elasticity and viscosity. In a human study involving five volunteers, viscoelastic anisotropy was assessed by rotating an ultrasound transducer 360°. An oval-shaped pattern in the elasticity of human forearm skin was identified, indicating the high elastic anisotropy of skin; the average elastic moduli were 24.90±6.63 and 13.64±2.67kPa along and across the collagen fiber orientation, respectively. The average viscosity of all the recruited volunteers was 3.23±0.93Pa·s. Although the examined skin exhibited elastic anisotropy, no evident viscosity anisotropy was observed.

Three-Dimensional Ultrasound Elastography Detects Age-Related Increase in Anterior Peripapillary Sclera and Optic Nerve Head Compression During IOP Elevation.

High-frequency ultrasound elastography offers a tool to resolve the complex and heterogeneous deformation through the full thickness of the optic nerve head (ONH) and peripapillary sclera (PPS). Using this tool, we quantified the three-dimensional deformation of the ONH and PPS in human donor eyes and evaluated age-associated changes. The ONH and PPS in 15 human donor globes were imaged with a 50-MHz ultrasound probe while increasing intraocular pressure (IOP) from 15 to 30 mmHg. Tissue displacements were obtained using correlation-based ultrasound speckle tracking. Three-dimensional spherical strains (radial, circumferential, meridional, and respective shear strains) were calculated for the ONH and PPS volumes segmented from three-dimensional ultrasound images. Age-related trends of different strains in each region of interest were explored. The dominant form of IOP-induced deformation in the ONH and PPS was radial compression. High-magnitude localized out-of-plane shear strains were also observed in both regions. Most strains were concentrated in the anterior one-half of the ONH and PPS. The magnitude of radial and volumetric strains increased with age in the anterior ONH and anterior PPS, indicating greater radial compression and volume loss during IOP elevation in older age. The age-associated increase of radial compression, the predominant form of IOP-induced deformation in anterior ONH and PPS, may underlie age-associated glaucoma risk. High-frequency ultrasound elastography offers a useful tool to quantify all types of deformation comprehensively in all regions of ONH and PPS, which may improve our understanding of the biomechanical factors contributing to glaucoma risk.

High-Frequency Ultrasound Elastography to Assess the Nonlinear Elastic Properties of the Cornea and Ciliary Body.

Mechanical properties of the anterior anatomical structures of the eye, such as the cornea and ciliary body, play a key role in the ocular function and homeostasis. However, measuring the biomechanical properties of the anterior ocular structures, especially deeper structures, such as the ciliary body, remains a challenge due to the lack of high-resolution imaging tools. Herein, we implement a mechanical shaker-based high-frequency ultrasound elastography technique that can track the induced elastic wave propagation to assess the linear and nonlinear elastic properties of anterior ocular structures. The findings of this study advance our understanding of the role of anterior ocular structures in the pathogenesis of different ocular disorders, such as glaucoma.

High-Frequency Ultrasound Elastography for Assessing Elastic Properties of Skin and Scars.

Scars are a type of fibrous tissue that typically forms during the wound healing process to replace damaged skin. Because studies have indicated a high correlation between scar stiffness and clinical symptoms, assessing the mechanical properties of scar is crucial for determining an appropriate treatment strategy and evaluating the treatment's efficacy. Shear wave elastography (SWE) is a common technique for measuring tissue elasticity. Because scars are typically a few millimeters thick, they are thin-layer tissues, and therefore, the dispersion effect must be considered to accurately estimate their elasticity. In this study, high-frequency ultrasound (HFUS) elastography was proposed for estimating the elastic properties of scars by using the Lamb wave model (LWM). An external vibrator was used to generate elastic waves in scar tissue and skin, and the propagation of the elastic waves was tracked through 40-MHz ultrafast ultrasound imaging. The elasticity was estimated through shear wave models (SWMs) and LWMs. The effectiveness of using HFUS elastography was verified through phantom and human studies. The phantom experiments involved bulk phantoms with gelatin concentrations of 7% and 15% and 2-4-mm-thick thin-layer 15% gelatin phantoms. The studies of three patients with eight cases of scarring were also conducted. The phantom experimental results demonstrated that the elasticity estimation biases for the thin-layer mediums were approximately -36% to -50% and 3% to -9% in the SWMs and LWMs, respectively, and the estimated shear moduli were 12.8 ± 5.4 kPa and 74.8 ± 26.8 kPa for healthy skin and scar tissue, respectively. All the results demonstrated that the proposed HFUS elastography has a great potential for improving the accuracy of elasticity estimations in clinical dermatological diagnoses.

High-frequency ultrasound elastography improves the effect of determining the nature of lesions during the diagnosis of Hashimoto's thyroiditis and thyroid cancer.

article: High-frequency ultrasound elastography improves the effect of determining the nature of lesions during the diagnosis of Hashimoto's Thyroiditis and thyroid cancer - Minerva Medica 2021 Sep 21 - Minerva Medica - Journals

Heartbeat-Induced Corneal Axial Displacement and Strain Measured by High Frequency Ultrasound Elastography in Human Volunteers.

PurposeThe purpose of this study was to establish in vivo data acquisition and processing protocols for repeatable measurements of heartbeat-induced corneal displacements and strains in human eyes, using a high-frequency ultrasound elastography method, termed ocular pulse elastography (OPE).MethodsTwenty-four volunteers with no known ocular diseases were recruited for this study. Intraocular pressure (IOP) and ocular pulse amplitude (OPA) were measured using a PASCAL Dynamic Contour Tonometer (DCT). An in vivo OPE protocol was developed to measure heartbeat-induced corneal displacements. Videos of the central 5.7 mm of the cornea were acquired using a 50-MHz ultrasound probe at 128 frames per second. The radiofrequency data of 1000 frames were analyzed using an ultrasound speckle tracking algorithm to calculate corneal displacements and quantify spectral and temporal characteristics. The intrasession and intersession repeatability of OPE- and DCT-measured parameters were also analyzed.ResultsThe in vivo OPE protocol and setup were successful in tracking heartbeat-induced corneal motion using high-frequency ultrasound. Corneal axial displacements showed a strong cardiac rhythm, with good intrasession and intersession repeatability, and high interocular symmetry. Corneal strain was calculated in two eyes of two subjects, showing substantially different responses.ConclusionsWe demonstrated the feasibility of high-frequency ultrasound elastography for noninvasive in vivo measurement of the cornea's biomechanical responses to the intrinsic ocular pulse. The high intrasession and intersession repeatability suggested a robust implementation of this technique to the in vivo setting.Translational RelevanceOPE may offer a useful tool for clinical biomechanical evaluation of the cornea by quantifying its response to the intrinsic pulsation.

High Frequency Ultrasound Elastography for Estimating the Viscoelastic Properties of the Cornea Using Lamb Wave Model.

Estimating the elasticity distribution in the cornea is important because corneal elasticity is usually influenced by corneal pathologies and surgical treatments, especially for early corneal sclerosis. Because the thickness of the cornea is typically less than 1 mm, high-resolution ultrasound elastography as well as the Lamb wave model is required for viscoelastic property estimation. In the present study, an array high-frequency ultrasound (HFUS) elastography method based on ultrafast ultrasound imaging was proposed for estimating the viscoelastic properties of porcine cornea. The elastic wave was generated by an external vibrator, after which the wave propagation image was obtained using a 40-MHz array transducer. Viscoelasticity estimation was performed by fitting the phase velocity curve using the Lamb wave model. The performance of the proposed HFUS elastography system was verified using 2-mm-thick thin-layer gelatin phantoms with gelatin concentrations of 7% and 12%. Ex vivo experiments were carried out using fresh porcine cornea with artificial sclerosing. Experimental results showed that the estimated elasticity was close to the standard value obtained in the phantom study when the Lamb wave model was used for elasticity measurement. However, the error between the standard elasticity values and the elasticity values estimated using group shear wave velocity was large. In the ex vivo eyeball experiments, the estimated elasticities and viscosities were respectively 9.1 ± 1.3 kPa and 0.5 ± 0.10 Pa·s for a healthy cornea and respectively 15.9 ± 2.1 kPa and 1.1 ± 0.12 Pa·s for a cornea with artificial sclerosis. A 3D HFUS elastography was also obtained for distinguishing the region of sclerosis in the cornea. The experimental results demonstrated that the proposed HFUS elastography method has high potential for the clinical diagnosis of corneal diseases compared with other HFUS single-element transducer elastography systems.

Characterization of the extensor digitorum communis tendon using high‐frequency ultrasound shear wave elastography

Hand tendon injuries caused by various accidents are common in emergency departments. The assessment of tendon properties is crucial for evaluating the effectiveness of therapy or rehabilitation during recovery after hand injuries. Many recent studies have indicated that the shear wave velocity (SWV) of tendons is related to their stiffness. However, measurement of SWV of hand tendon is still a challenge because the small size of tendon and the limitation of existing ultrasound systems for detecting fast SWV. We propose a high-frequency ultrasound (HFUS) elastography system using an external vibrator to measure the SWV of the extensor digitorum communis (EDC) tendon. First, animal studies were performed by measuring the SWV and stress of porcine tendons using the proposed HFUS elastography and materials testing systems respectively. In the human experiment, SWVs were measured during hand extension and flexion. The applied stress from a finger during the movements was recorded synchronously by using a load cell. The experimental results reveal that a favorable linear correction (R2 of 0.96) was obtained between tendon SWV and stress in animal studies. In the human (hand) EDC tendon experiments, the SWV increased with the extension and flexion of the hand. The SWV of the EDC tendon was in the range of 20 to 135m/s as the applied force from the finger of a healthy human increased to 50% maximal voluntary contraction. All the experimental results show that the proposed HFUS elastography system can be used to characterize the EDC tendon and has potential use for evaluating tendon stiffness during recovery after hand injures.

Three-Dimensional Inflation Response of Porcine Optic Nerve Head Using High-Frequency Ultrasound Elastography.

Characterization of the biomechanical behavior of the optic nerve head (ONH) in response to intraocular pressure (IOP) elevation is important for understanding glaucoma susceptibility. In this study, we aimed to develop and validate a three-dimensional (3D) ultrasound elastographic technique to obtain mapping and visualization of the 3D distributive displacements and strains of the ONH and surrounding peripapillary tissue (PPT) during whole globe inflation from 15 to 30 mmHg. 3D scans of the posterior eye around the ONH were acquired through full tissue thickness with a high-frequency ultrasound system (50 MHz). A 3D cross-correlation-based speckle-tracking algorithm was used to compute tissue displacements at ∼30,000 kernels distributed within the region of interest (ROI), and the components of the strain tensors were calculated at each kernel by using least square estimation of the displacement gradients. The accuracy of displacement calculation was evaluated using simulated rigid-body translation on ultrasound radiofrequency (RF) data obtained from a porcine posterior eye. The accuracy of strain calculation was evaluated using finite element (FE) models. Three porcine eyes were tested showing that ONH deformation was heterogeneous with localized high strains. Substantial radial (i.e., through-thickness) compression was observed in the anterior ONH and out-of-plane (i.e., perpendicular to the surface of the shell) shear was shown to concentrate in the vicinity of ONH/PPT border. These preliminary results demonstrated the feasibility of this technique to achieve comprehensive 3D evaluation of the mechanical responses of the posterior eye, which may provide mechanistic insights into the regional susceptibility in glaucoma.

Characterization of Hand Tendons Through High-Frequency Ultrasound Elastography.

Tendon stiffness plays an important role in the tendon healing process, and many studies have indicated that measuring the shear wave velocity (SWV) on tendons relates to their stiffness. Because the thickness of hand tendons is a few millimeters, high-resolution imaging is required for visualizing hand tissues. However, the resolution of current ultrasound elastography systems is insufficient. In this study, a high-frequency (HF) ultrasound elastography system is proposed for measuring the SWVs of hand tendons. The HF ultrasound elastography system uses an external vibrator to create shear waves on hand tendons. Then, it uses a 40-MHz HF ultrasound array transducer with ultrafast ultrasound imaging technology to measure the SWV for characterizing hand tendons. A handheld device that combines a transducer and a vibrator allows the user to scan hand tissues. The biases of HF ultrasound elastography were measured in gelatin phantom experiments and were less than 6% compared to standard mechanical testing approach. Human experiments showed the ability to use HF ultrasound elastography to distinguish different SWVs of hand tendons. The SWVs were 0.73 ± 0.65 m/s and 1 ± 0.54 m/s for flexor digitorum superficialis (FDS) and flexor digitorum profundus (FDP), respectively, and 0.52 ± 0.14 m/s and 4.02 ± 0.77 m/s for extensor tendon under stretch and contraction conditions, respectively. The simplicity and convenience of the HF ultrasound elastography system for measuring hand tendon stiffness make it a promising tool for evaluating the severity of hand injuries and the performance of rehabilitation after hand injuries.

Ex Vivo Evaluation of Mouse Brain Elasticity Using High-Frequency Ultrasound Elastography.

Most neurodegenerative diseases are highly linked with aging. The mechanical properties of the brain should be determined for predicting and diagnosing age-related brain diseases. A preclinical animal study is crucial for neurological disease research. However, estimation of the elasticity properties of different regions of mouse brains remains difficult because of the size of the brain. In this paper, high-frequency ultrasound elastography (HFUSE) based on shear wave imaging was proposed for mapping the stiffness of the mouse brain at different ages ex vivo. For HFUSE, a 40-MHz ultrasound array transducer with an ultrafast ultrasound imaging system was used in this paper. The accuracy and resolution during HFUSE were determined through a mechanical testing system and by conducting phantom experiments. In the experiments, the error in the elastic modulus measurement was approximately 10% on average, and the axial resolution was 248μm. Animal testing was conducted using mice that were 4 (young aged) and 11 (middle aged) months old. The elasticity distributions of the cortex and hippocampus in the mouse brains were obtained through HFUSE. The average shear moduli of the cortex and hippocampus were 3.84 and 2.33kPa for the 4-month-old mice and 3.77 and 1.94kPa for the 11-month-old mice, respectively. No statistical difference was observed in the cortex stiffness of mice of different ages. However, the hippocampus significantly softened with aging.

Measurement of surface acoustic waves in high-frequency ultrasound: Preliminary results.

Skin lesions change elastic properties near the surface. In the last decades, several non-invasive elastography techniques have been developed for detecting the mechanical properties of tissue. In particular, harmonic elastography is characterized for inducing shear wave propagation by an external vibrator in order to estimate shear modulus. However, near the boundary region, propagation is governed by surface acoustic waves (SAW). This paper combines crawling waves elastography with a high-frequency ultrasound (HFUS) system for the estimation of the SAW-to-shear compensation factor when ultrasound (US) gel is used as coupling interface. Experiments explore the SAWspeed in a homogeneous phantom with a solid-water interface in order to corroborate theoretical findings. Subsequently, experiments in a solid-US gel interface are conducted in order to find the correct compensation factor. Preliminary results suggest that SAW propagation can be detected using HFUS, and shear velocity maps can be generated by applying the estimated empirical correction factor. This study will potentially avoid the underestimation of shear modulus when using SAW-based HFUS elastography which is promising for the better diagnosis of skin diseases.