High Fertility Sites Research Articles

Basin-wide variations in foliar properties of Amazonian forest: phylogeny, soils and climate

Abstract. We analysed 1040 individual trees, located in 62 plots across the Amazon Basin for leaf mass per unit area (MA), foliar carbon isotopic composition (δ13C) and leaf level concentrations of C, N, P, Ca, Mg, K and Al. All trees were identified to the species level with the dataset containing 58 families, 236 genera and 508 species, distributed across a wide range of soil types and precipitation regimes. Some foliar characteristics such as MA, [C], [N] and [Mg] emerge as highly constrained by the taxonomic affiliation of tree species, but with others such as [P], [K], [Ca] and δ13C also strongly influenced by site growing conditions. By removing the environmental contribution to trait variation, we find that intrinsic values of most trait pairs coordinate, although different species (characterised by different trait suites) are found at discrete locations along a common axis of coordination. Species that tend to occupy higher fertility soils are characterised by a lower MA and have a higher intrinsic [N], [P], [K], [Mg] and δ13C than their lower fertility counterparts. Despite this consistency, different scaling patterns were observed between low and high fertility sites. Inter-relationships are thus substantially modified by growth environment. Analysing the environmental component of trait variation, we found soil fertility to be the most important predictor, influencing all leaf nutrient concentrations and δ13C and reducing MA. Mean annual temperature was negatively associated with leaf level [N], [P] and [K] concentrations. Total annual precipitation positively influences MA, [C] and δ13C, but with a negative impact on [Mg]. These results provide a first basis for understanding the relationship between the physiological functioning and distribution of tree species across Amazonia.

Plant traits link hypothesis about resource-use and response to herbivory

Abstract Grazing by large herbivores, in interplay with environmental productivity, is a key driver of the composition of the vegetation with important consequences on the ecosystem and, consequently, for land management. We tested the predictions of the resource availability – resource–acquisition theory by assessing the extent to which community averages of plant traits, known to be related to plant growth, competitive ability and response to grazing were correlated with resource gradients within local (200 km 2 ) geographical ranges. Second, we assessed the applicability of the same set of plant traits to make inferences on ecological effects of grazing by sheep in alpine ecosystems in Norway, using a data set consisting of 16 sites in central Norway. We estimated grazing intensity by free-ranging sheep based on GPS telemetry, soil properties, plant species composition and species traits i.e. specific leaf area (SLA), leaf dry matter content (LDMC), leaf size and plant height. Soil fertility and the interaction between soil fertility and grazing, but not grazing intensity alone, were significantly related to plant species and traits composition. Generally, average SLA showed lower correspondence with soil fertility and grazing than the other traits. Leaf size and plant height were lowest at sites with high grazing intensity and in sites with low fertility, and increased with soil fertility in little and moderately grazed sites, but declined at high fertility sites when grazing was intense. LDMC showed the opposite trend. Grazing intensity was more related to the variability in plant composition and average plant traits when environmental productivity was high. Our results therefore are indicative of a convergence of responses to grazing and nutrient limitation.

Nitrogen fixation in mixed Hylocomium splendens moss communities

The pleurocarpus feather moss, Hylocomium splendens, is one of two co-dominant moss species in boreal forest ecosystems and one of the most common mosses on earth, yet little is known regarding its capacity to host cyanobacterial associates and thus contribute total ecosystem N. In these studies, we evaluated the N-fixation potential of the H. splendens-cyanobacteria association and contrasted the N-fixation activity with that of the putative N-fixing moss-cyanobacteria association of Pleurozium schreberi. Studies were conducted to: quantify N-fixation in H. splendens and P. schreberi in sites ranging from southern to northern Fennoscandia; assess N and P availability as drivers of N-fixation rates; contrast season-long N-fixation rates for both mosses; and characterize the cyanobacteria that colonize shoots of H. splendens. Nitrogen-fixation rates were generally low at southern latitudes and higher at northern latitudes (64-69 degrees N) potentially related to anthropogenic N deposition across this gradient. Nitrogen fixation in H. splendens appeared to be less sensitive to N deposition than P. schreberi. The season-long assessment of N-fixation rates at a mixed feather moss site in northern Sweden showed that H. splendens fixed a substantial quantity of N, but about 50% less total N compared to the contribution from P. schreberi. In total, both species provided 1.6 kg fixed N ha(-1) year(-1). Interestingly, H. splendens demonstrated somewhat higher N-fixation rates at high fertility sites compared to P. schreberi. Nostoc spp. and Stigonema spp. were the primary cyanobacteria found to colonize H. splendens and P. schreberi. These results suggest that H. splendens with associated Nostoc or Stigonema communities contributes a significant quantity of N to boreal forest ecosystems, but the contribution is subordinate to that of P. schreberi at northern latitudes. Epiphytic cyanobacteria are likely a key factor determining the co-dominant presence of these two feather mosses across the boreal biome.

Soil amino-acid availability across a temperate-forest fertility gradient

Despite increasing recognition that free amino acids can be an important source of N for plant uptake, we have a poor understanding of environmental variation in the availability of amino-acid N in soils outside of arctic, alpine and boreal regions. I investigated patterns of amino-acid availability along a temperate forest fertility gradient ranging from low mineral N availability, oak-dominated forests to high mineral N availability, maple-basswood forests (5 sites). I measured standing pools of free amino acids, soluble peptides, ammonium and nitrate, rates of amino acid production (native proteolysis activity) and rates of consumption of a 15N-labeled leucine tracer. Standing pools of amino acid N decreased consistently along the fertility gradient from the low fertility black oak/white oak system to the high fertility sugar maple/basswood system, with a 25-fold difference in pool sizes between the poorest and richest sites. Standing pools of soluble peptides varied little among sites, instead, the relationship between free amino acids and peptides changed markedly across the gradient. At low fertility sites free amino acids were positively correlated with soluble peptides, whereas free amino acid pools were universally low at high fertility sites, regardless of peptide pools. Assays for native proteolysis activity indicated that amino acid production did not vary significantly among sites. Recovery of leucine tracer in inorganic (NH4 + and NO3 −) pools and in residual soil organic matter both increased with increasing soil fertility; however, total consumption of the added amino-acid tracer did not vary among sites. Results from this study demonstrate that free amino acids can make an important contribution to potentially plant-available N pools in temperate forest soils, particularly at low fertility sites.

Identification of boreal forest stands with high herbaceous plant diversity using airborne laser scanning

Boreal forest stands with high herbaceous plant species diversity have been found to be one of the main habitats for many endangered species, but the locations and sizes of these herb-rich forest stands are not well known in many areas. Better identification of the stands could improve both their conservation and management. A new approach is proposed here for locating the mature herb-rich forest stands using airborne laser scanner (ALS) data and logistic regression, or the k-NN classifier. We show that ALS technology is capable of distinguishing the ecologically important herb-rich forests from those growing on less fertile site types, mainly on the basis of unique but quantifiable crown structure and vertical profile that characterise forests on high fertility sites. The study site, Koli National Park, is located on the border of the southern and middle boreal vegetation zones in Finland, and includes 63 herb-rich forest stands of varying sizes. The model and test data comprised 274 forest stands belonging to five forest site types varying from very fertile to poor. The best overall classification accuracy achieved with the k-NN method was 88.9%, the herb-rich forests being classified correctly in 65.0% of cases and the other forest site types in 95.7%. The best overall classification accuracy achieved with logistic regression was 85.6%, being 55.0% for the herb-rich forests and 94.3% for the other forest site types. Both methods demonstrated promising potential for separating herb-rich forests from other forest site types, although slightly better results were obtained with the non-parametric k-NN method, which was capable of utilising a higher number of explanatory variables. It is concluded that ALS-based data analysis techniques are applicable to the detection of mature boreal herb-rich forests in large-scale forest inventories.

Effects of eutrophication on salt marsh root and rhizome biomass accumulation

The root:shoot ratio of grassland plants may be lower in high fertility sites than in low fertility sites as plants modify their root, rhizome and shoot morphologies (and masses) to suit prevail- ing nutrient availability. We conducted geographically diverse and regionally specific field sampling and measured above- and belowground plant biomasses in western Atlantic and Gulf of Mexico salt marshes to determine whether there is a similar morphological response in Spartina alterniflora, the dominant salt marsh plant. Coastal nutrient addition/enrichment, which is widespread and ongoing, may lower root and rhizome biomass, belowground production and organic accumulation in this spe- cies. Higher soil respiration and a lower Eh (redox potential) are expected additional soil property changes. The addition of P, more than of N, seems to reduce root and rhizome biomass accumulation. The cumulative effects of increased nutrient loadings on salt marshes may be to decrease soil eleva- tion and accelerate the conversion of emergent plant habitat to open water, particularly on the lower marsh. The recommendations for management practices intended to conserve coastal marshes include reducing nutrient loading to coastal zones, solving water quality problems with a multiple nutrient approach, and choosing monitoring metrics that are based on both above- and belowground plant biomass.

Improved quality of beneath‐canopy grass in South African savannas: Local and seasonal variation

AbstractQuestions:Do large trees improve the nutrient content and the structure of the grass layer in savannas? Does the magnitude of this improvement differ with locality (soil nutrients) and season (water availability)? Are grass structure and species composition beneath tree canopies influenced by soil fertility and season?Location:South Africa.Methods:We compared grass leaf nutrient contents and grass sward structure beneath and outside tree canopy areas in three savannas of different soil fertility during the dry and the wet seasons.Results:Grass nitrogen contents were twice as high during the wet season as compared to the dry season, being more strongly elevated underneath tree canopies during the wet season. Grasses had significantly less stem material and provided less dead material underneath trees on the high soil fertility site. Grass species composition differed significantly beneath and outside tree canopies, with more nutritious grass species found sub‐canopy. Grass species richness was significantly lower beneath than outside of trees at the site of high soil fertility.Conclusions:Trees improve overall quality of savanna grasses by enhancing grass growth and nutrient uptake during the wet season, and by delaying grass wilting in the dry season. The positive effect of trees on the grass layer might attract grazing herbivores in otherwise nutrient‐poor savannas. Hence, single standing large trees should be maintained to sustain high grass quality and, consequently, grazer populations in savanna habitats.

Intra-specific variation in cell wall constituents of needle age classes of Pinus sylvestris in relation to soil fertility status in Southwest England

First, second and third year needles were collected from the same branches of young Scots pine trees growing on soils of two different status (high and low fertility sites) that varied in mineral nutrient concentrations and N mineralisation potential. All needle age classes were analysed for total carbon, acid detergent fibre (ADF), lignin, cellulose, phenylpropanoid derivatives (PPD) of lignin, sugar constituents of non-crystalline, hydrated cellulose and hemicellulose, and nutrient concentrations (N, P, K, Ca, Mg and Mn). Significant intra-specific variation in the litter quality variables in relation to soils of high and low fertility was found in the second and third year needles, whereas there were no differences in the other cell wall constituents and mineral elements of the first year needles. The second and third year needles from the low fertility soil contained higher concentrations of ADF, lignin, cellulose, sugar constituents of non-crystalline, hydrated cellulose and hemicellulose, and phenylpropanoid derivatives (PPD) of lignin, but lower concentrations of N, P and Mg than the same needles from the high fertility and fertilised soils. The results in the present study indicate that under different soil fertilities, needle age classes show significant variations in the cell wall constituents and mineral elements, and suggest that this can result in significant variation in litter quality and decomposition rates.

Modeling climate change effects on the potential production of French plains forests at the sub-regional level

We modeled the effects of climate change and two forest management scenarios on wood production and forest carbon balance in French forests using process-based models of forest growth. We combined data from the national forest inventory and soil network survey, which were aggregated over a 50 x 50-km grid, i.e., the spatial resolution of the climate scenario data. We predicted and analyzed the climate impact on potential forest production over the period 1960-2100. All models predicted a slight increase in potential forest yield until 2030-2050, followed by a plateau or a decline around 2070-2100, with overall, a greater increase in yield in northern France than in the south. Gross and net primary productivities were more negatively affected by soil water and atmospheric water vapor saturation deficits in western France because of a more pronounced shift in seasonal rainfall from summer to winter. The rotation-averaged values of carbon flux and production for different forest management options were estimated during four years (1980, 2015, 2045 and 2080). Predictions were made using a two-dimensional matrix covering the range of local soil and climate conditions. The changes in ecosystem fluxes and forest production were explained by the counterbalancing effect of rising CO2 concentration and increasing water deficit. The effect of climate change decreased with rotation length from short rotations with high production rates and low standing biomasses to long rotations with low productivities and greater standing biomasses. Climate effects on productivity, both negative and positive, were greatest on high fertility sites. Forest productivity in northern France was enhanced by climate change, increasingly from west to east, whereas in the southwestern Atlantic region, productivity was reduced by climate change to an increasing degree from west to east.

The stiffness modulus in Norway spruce as a function of year ring

AbstractThe elastic properties of Norway spruce were studied by means of ultrasound. Samples were from stems that were grown either at a medium- or a high-fertility site in Finland. The stiffness modulus was determined in the longitudinal, radial, and tangential directions as a function of the distance from the pith using 1-mm-thick samples. Tangentially cut samples contained only earlywood, while radially and longitudinally cut samples contained both earlywood and latewood. The stiffness modulus in the longitudinal direction for Norway spruce grown at a medium-fertility site increased with distance from the pith from 6±1 to 8.5±1 GPa. This parameter increased as the mean microfibril angle decreased. In the radial and tangential directions, the stiffness modulus was nearly constant, at 1±0.15 and 0.35±0.04 GPa, respectively. The longitudinal stiffness modulus of fast-growing clonal trees, which contained a large share of juvenile wood and were growing on a fertile site, was ca. 30% smaller (4±1 GPa near the pith and 6±1 GPa at the age of 12 years) than that of trees on a medium-fertility site. No difference was found in the radial and tangential stiffness modulus between stems grown at the two sites.

Tree seedling growth, survival, and morphology in response to landscape-level variation in soil resource availability in northern Michigan

To investigate causes of tree species distributions across soil resources in northern Michigan, we conducted a seedling transplant experiment with five species showing different site affinities: Acer saccharum Marsh. (sugar maple), Prunus serotina Ehrh. (black cherry), and Fraxinus americana L. (white ash), which are associated with high-fertility mesic moraine; Quercus rubra L. (red oak), associated with intermediate sites; and Quercus velutina Lam. (black oak), associated with low-fertility droughty outwash sites. Seedlings were planted in plots stratified across variation in light and soil nutrient and water availability. After one growing season, under 14%–27% canopy openness, species tended to trade off between high survival on outwash versus high relative growth rate of root + stem mass (RGRrs) on moraine. The high survivorship of black and red oak on outwash was associated with greater root and whole-plant mass in comparison with sugar maple, white ash, and black cherry. High RGRrs on high-fertility moraine for these latter species was associated with high fine root area per unit whole-plant mass and plasticity to increase specific root area in response to increased soil resources. We did not detect a similar survival–growth trade-off for seedlings grown at lower light (3%–10%) on intermediate versus high-fertility sites. Overall, these results suggest that species distributions across soil resource gradients can in part be explained by a trade-off between tolerance of low soil resources versus competitive ability (i.e., growth) under high soil resources.

Comparison of two coniferous plantations in central Japan with respect to forest productivity, growth phenology and soil nitrogen dynamics

Japanese cypress ( Chamaecyparis obtusa Endl.) and Japanese cedar ( Cryptomeria japonica D. Don) are common species for plantation forestry in Japan. Cypress is conventionally planted on sites of low fertility whereas for cedar high fertility sites are used. Objectives of this study were to compare the productivities of cypress and cedar plantations grown on adjacent sites where common properties of soils, such as pH values and C and N contents, were similar, and to relate the N cycling at their site with productivities. The stem diameter of trees, aboveground litter production and fine root biomass were measured as indices of forest productivity. Parameters of N cycling included pools of total N and mineral N (ammonium + nitrate), annual N leaching, and potentially mineralizable N. The radial stem increment of the two tree species was similar. However, cedar site had higher total basal area and annual basal increment than cypress site reflecting higher tree density on the cedar site. Aboveground litter, fine root biomass, soil organic matter, and N turnover were higher on the cedar site than on the cypress site. However, litter production and fine root biomass per unit basal area was greater at the cypress site. Phenological pattern of stem growth and periodical litter production were similar for both species during the study period (1992–2000), but showed distinct annual variations caused by the fluctuation in the ambient temperature and precipitation. Mineral N content and the N mineralization potential were greater on the cedar site, indicating greater N availability and higher total tree productivity at the cedar site than those at the cypress site. When provided with more space in the canopy to expand more needles and in the soil to develop more fine roots to exploit sufficient resources, the individual cypress trees have the potential to grow faster. On fertile site and at lower tree density, thicker logs of cypress might be yielded.

Slits in container wall improve root structure and stem straightness of outplanted Scots pine seedlings

Root structure and basal sweep were measured on 6-year-old Scots pine (Pinus sylvestris L.) trees at two sites with different soil fertility. Each site was planted with seedlings of identical origin after nursery cultivation in either solidwall container types with vertical ribs or in slitwall container types. Neither container design nor container volume affected tree height or stem diameter on the two sites. The transversal area of lateral roots was larger than the transversal area of bottom roots for the two container types at both sites. The proportion of bottom root transversal area to the total root transversal area was larger in the seedlings growing on the low fertility site than in those growing in the high fertility site for both container types. Seedlings cultivated in slitwall containers had a larger root area in proportion to stem diameter and had less root spiralling compared to the trees cultivated in solidwall containers. At the high fertility site, trees from the slitwall container type had straighter stem bases than seedlings grown in solidwall containers. At the low fertility site, differences in basal sweep formation were small between the container types. Reasons for this are discussed.

Walnut demonstrates strong genetic variability for adaptive and wood quality traits in a network of juvenile field tests across Europe

Adaptive and wood quality trait data were collected and analyzed on commercially available Juglans regia and J. regia×J. nigra provenances and progenies planted across Europe in a multi-site network. A total of 19 seed sources, replicated 35 times per site, were planted at 13 sites from 5 European countries, encompassing the potential distribution area of timber production plantation sites. The following traits were evaluated: survival, height, diameter at breast height, stem form, apical dominance, vegetative budbreak, along with biotic and abiotic damage. Mean values were significantly different both among provenances/progenies and sites. Most common damage was late spring and early autumn frost. Bud break ranking was significantly correlated with provenance and progeny origin. Although J. regia is fast growing, southern European early budbreak plant material should not be planted under most middle European conditions where late spring frost can be expected, as it has a significant negative impact on architectural (and thus wood quality) traits. Hybrid J. regia×J. nigra progeny performed better than J. regia provenances/progenies for most traits measured. Differences were significantly in favor of hybrids at sites with medium to low fertility, although some locally selected seed sources tended to perform as well as hybrids on high fertility sites.

Contribution of amino compounds to dissolved organic nitrogen in forest soils

Dissolved organic nitrogen (DON) may play an important role in plantnutrition and nitrogen fluxes in forest ecosystems. In spite of the apparentimportance of DON, there is a paucity of information concerning its chemicalcomposition. However, it is exactly this chemical characterization that isrequired to understand the importance of DON in ecosystem processes. Theprimaryobjective of this study was to characterize the distribution of free aminoacidsand hydrolyzable peptides/proteins in the DON fraction of Oa horizon leachatesalong an extreme edaphic gradient in northern California. Insitu soil solutions were extracted by centrifugation from Oahorizonscollected beneath Pinus muricata (Bishop pine) andCupressus pygmaea (pygmy cypress) on slightlyacidic/fertile and highly acidic/infertile sites. DON accounted for 77 to99% of the total dissolved nitrogen in Oa horizon leachates. Nitrogen infree amino acids and alkyl amines ranged from 0.04–0.07 mgN/L on the low fertility site to 0.45–0.49 mg N/L onthe high fertility site, and accounted for 1.5 to 10.6% of the DON fraction.Serine, glutamic acid, leucine, ornithine, alanine, aspartic acid andmethylamine were generally the most abundant free amino compounds. Combinedamino acids released by acid hydrolysis accounted for 48 to 74% of theDON, suggesting that proteins and peptides were the main contributor to DON inOa horizon leachates. Together, nitrogen from free andcombined amino compounds accounted for 59 to 78% of the DON. Most of theDON was found in the hydrophobic fraction, which suggests the presence ofprotein/peptide-polyphenol complexes or amino compounds associated withhumic substances. Because free and combined amino acids can be an importantnitrogen source for some plants, soil DON may play an important role in plantnutrition and ecosystem function.

The role of trees in enhancing soil nutrient availability for native perennial grasses in open eucalypt woodlands of north-east Queensland

To investigate the effects of eucalypt trees on pasture in open eucalypt woodlands of north-eastern Queensland, 2 common native pasture species, Chrysopogon fallaxand Heteropogon contortus, were grown in soil collected from under tree canopies and inter-canopy areas. These soils were collected from 2 localities that differed in soil fertility. The objective was to determine whether trees enhanced soil nutrient levels beneath their canopies and whether such changes affected pasture productivity and quality. It was hypothesised that tree effects would be greater when overall soil fertility was low. Shade and water stress treatments, which aimed to simulate field conditions, were also imposed to investigate their independent and interactive effects on plant growth. Chemical analyses showed that nutrient levels were higher in under-canopy soils, and plants grown in under-canopy soil produced 42% more biomass than plants in outside-canopy soil. This increase in biomass was significantly greater when the soil was from the low fertility site than from the high fertility site. Leaf quality, in terms of N and P concentration and dry matter digestibility (DMD), was generally higher in plants in under-canopy soil. Shading reduced plant root biomass but had no effects on above ground biomass or leaf quality. Water stress reduced above and below ground biomass and increased leaf quality. Shading and water stress effects were greater in plants in the more fertile soil types. Simulated ‘under-canopy’ plants (shaded, water-stressed plants in under-canopy soil) produced more biomass and had higher leaf N concentration and DMD than simulated ‘outside-canopy’ plants (unshaded, well-watered plants in outside-canopy soil). In a pastoral context, trees in tropical woodlands are generally considered in terms of their competitive effect on pasture productivity. This study has demonstrated that soil nutrient availability is enhanced under eucalypt canopies. The biomass results indicate that this effect is greater when overall soil fertility conditions are low. These enhanced soil nutrient conditions influence forage productivity and quality. Such positive benefits should be considered when management decisions are taken to remove or kill trees in eucalypt woodlands.

Influence of second flushing on genetic assessment of cold hardiness in coastal Douglas-fir ( Pseudotsuga menziesii var. menziesii (Mirb.) Franco)

The consequences of second flushing for fall cold hardiness in coastal Douglas-fir ( Pseudotsuga menziesii var. menziesii (Mirb.) Franco) was investigated in 4-year-old trees from two genetic tests: 42 polycross families in British Columbia and 8 full-sib families in the state of Washington. Cold injury to needle and stem tissues was assessed in October and November in samples of both second-flushed and non-second-flushed shoots from the same trees following artificial freezing. Freeze damage to needles and stems in the second-flushed portion of shoots was 50–60% greater than in non-second-flushed shoots. Great care, therefore, should be taken to consistently sample the same shoot type when comparing cold hardiness of genotypes (or families) using artificial freeze testing. However, because the estimated genetic correlations in freeze injury between second-flushed and non-second-flushed shoots were moderately positive, scoring all trees for hardiness of one shoot type should provide fairly accurate rankings of genotypes for cold hardiness of both shoot types. We recommend scoring non-second-flushed shoots because the frequency of second flushing decreases relatively rapidly with increasing age in coastal Douglas-fir. Hardening of both second-flushed and non-second-flushed shoots was delayed in trees with higher proportions of second-flushed shoots in their crown. Thus, foresters should avoid planting families with high propensity to second flush on high fertility sites (i.e. sites that promote second flushing) susceptible to fall frost events.

Frequent grazing by sheep reduced caucasian clover cover and rhizome mass in ryegrass pasture

The responses of hexaploid caucasian clover (Trifolium ambiguum) to four contrasting grazing regimes were compared with those of white clover (T. repens) in an endophytic (Neotyphodium lolii) hybrid ryegrass pasture on a fertile lowland site. After 2 years, frequent grazing (set stocking) by sheep reduced caucasian clover cover to 10% compared with 25.5% in infrequent grazing (rotational grazing) treatments (mean spelling time 25 days). Similarly, frequent grazing reduced caucasian clover rhizome plus root dry weight (780 kg DM/ha when sampled to 100 mm depth in frequently grazed plots, compared with 3220 kg DM/ha for infrequent). Under frequent grazing treatments, mean white clover cover was 21%, under infrequent hard grazing it was 26% and under lax infrequent grazing it was 14%. The reduction in ryegrass tiller population from 5720/m2 in the infrequently and laxly grazed treatments to 4150/ m2 in the frequently hard grazed pastures indicated the severity of that hard grazed treatment. These results show that in lowland ryegrass pastures on high fertility sites, the stoloniferous growth form of white clover may be superior to the rhizomatous strategy of caucasian clover when grazing by sheep is frequent throughout spring, summer and autumn. Keywords: caucasian clover, cover, grazing frequency, grazing intensity, kura clover, rhizomes, ryegrass, Trifolium ambiguum, Trifolium repens, white clover

On the relationship between nutrient use efficiency and fertility in forest ecosystems.

The concept of nutrient use efficiency is central in understanding ecosystem functioning because it is the step in which plants can influence the return of the nutrients to the soil pool and the quality of the litter. There are several ways to define nutrient use efficiency, but a common way within ecosystem ecology is as the ratio of litterfall production per unit nutrient to the litterfall nutrient content. However, this ratio is not a valid measurement to examine nutrient use efficiency in relationship to ecosystem fertility because there is a strong autocorrelation between litterfall dry mass per unit of nutrient and the amount of nutrients. More appropriate statistical analysis of the relationship between the fertility of ecosystems and the amount of nutrients in the litterfall are inconclusive, but indicate that, at least in some cases, there is (1) no pattern, (2) higher nutrient use efficiency at intermediate-fertility sites or (3) higher efficiency at higher-fertility sites. There is, however, no indication that nutrient use efficiency is greater in nutrient-poor ecosystems. This conclusion has important consequences for ecosystem nutrient cycling. Given the lack of a clear, consistent relationship between site fertility and litterfall nutrients, there is little likelihood that such a feedback mechanism plays an important role in ecosystem nutrient cycling.

SHORT COMMUNICATION: Soil microbial biomass C, N mineralization, and N uptake by corn in dairy cattle slurry- and urea-amended soils

A spring application of dairy cattle slurry (300 kg total N ha−1) on high- and low-fertility sites resulted in higher microbial biomass C during the growing season than on a control soil or a soil receiving 100 kg N ha−1 as urea. Microbial biomass C was also significantly higher on the high-fertility site and was reflected in greater N mineralization and N uptake by corn. There was no greater net N mineralization in the manured soil than in the control or fertilized soil as would be expected as a result of higher microbial biomass C and significant organic N contribution from the manure. Key words: Animal manure, nitrogen mineralization, corn, grain yields, soil fertility