The high energy and high frequency electro-optic Q-switching laser with alternating output of energy amplitude was the first reported by the temporal delay of pulse energy in 1064 nm laser. The experimental schematic of the output 1064 nm laser with different trigger time were obtained when turn on the Q-switch on the 60 μs. When turn on the Q-switch on the 71 μs, the pulse energy of 1064 nm is distributed in a “high-low” pulse sequence: the pulse energy of the 1st was higher than 2nd, and the 2nd pulse energy was lower than the 3rd is formed repeatedly, when turn on the Q-switch on the 48 μs, the output process of the pulse laser is reversed. To the best of our knowledge, it is the first time to report the high energy and high frequency electro-optic Q-switching laser with alternating output of energy amplitude. In experiment a repetition rate of 100 kHz with a pulse width of 13 ns was achieved when the pump power was 24 J and the charging voltage is 650 V, the total output power of 0.8 J was obtained and the conversion efficiency is 0.3%. And the result of the photon density distributions and the inverse population by numerical calculations are basically agree with the experiment result.