High Ecological Value Research Articles

Different hydraulic and photosynthetic responses to summer drought between newly sprouted and established Moso bamboo culms.

The subtropical regions in China are prone to recurrent summer droughts induced by the Western Pacific Subtropical High-Pressure, which has induced the death of tens of millions of culms of Moso bamboo (Phyllostachys edulis (Carriere) J. Houzeau), a widely distributed giant bamboo with high economic and ecological values. In the future, the intensity and frequency of the summer drought are projected to increase in these areas due to global climate change, which may lead to significant age-specific mortality of Moso bamboo. So far, it is still unclear about the age-specific response mechanisms of hydraulic traits and carbon balance of Moso bamboo when it is suffering to an ongoing summer drought. This study aimed to investigate the hydraulic and photosynthetic responses of newly sprouted (1 year old) and established (2-5 years old) culms of Moso bamboo to summer drought, which was manipulated by throughfall reduction in Lin'an of Zhejiang. The results showed that both newly sprouted and established culms had a gradually weakening hydraulic conductivity and photosynthesis during the whole drought process. In the early stage of the manipulated drought, the established culms had more loss of hydraulic conductivity than the newly sprouted culms. However, the newly sprouted culms had significant more loss of hydraulic conductivity and lower photosynthetic rates and stomatal conductance in the middle and late stages of the manipulated drought. The results suggest that the newly sprouted culms were more susceptible to summer drought than established culms due to the combined effects of hydraulic damage and photosynthetic restriction, explaining why the newly sprouted culms have higher mortality than elder culms when subjected to extreme drought. These findings provided insights into the mechanisms of Moso bamboo's age-specific drought-induced mortality, which will help for the anti-drought management of bamboo.

Transcriptome analysis of digestive diverticula of Hong Kong oyster (Crassostrea hongkongesis) infected with Vibrio harveyi

The Hong Kong oyster (Crassostrea hongkongesis), as the main marine aquaculture shellfish in the South China Sea, not only has high economic and ecological value, but also is an ideal model for conducting research on pathogen host interaction. However, diseases caused by Vibrio pose a serious impediment to the culture of C. hongkongesis. In this study, we performed transcriptome analysis of digestive diverticula of C. hongkongesis infected with V. harveyi. A total of 977, 689, 912 high quality reads and 955, 208, 562 valid reads were obtained. At 12, 24, 48 and 72 h post-infection, 1402, 2168, 2727 and 1398 differentially expressed genes (DEGs) were captured, respectively. GO enrichment analysis showed that DEGs were significantly enriched in cellular processes, catalytic activity, cell part and other terms. KEGG enrichment analysis revealed that these DEGs were mainly closely related to Necroptosis, RIG-I-like receptor signaling pathway, NF-kappa B signaling pathway, Toll-like receptor signaling pathway and other pathways are related. The results of WGCNA analysis indicated that THBS1, CA10, Trpm2, THAP12, PTPRT, HSPA12A, and ADAM10 were the hub genes in the gene co-expression network. This study will provide new ideas at the transcriptome level for the immune regulatory mechanisms and adaptability of the C. hongkongesis to V. infection, as well as for achieving selective breeding for Vibrio resistance in the C. hongkongesis.

Monitoring of Oil Spill Risk in Coastal Areas Based on Polarimetric SAR Satellite Images and Deep Learning Theory

Healthy coasts have a high ecological service value. However, many coastal areas are faced with oil spill risks. The Synthetic Aperture Radar (SAR) remote sensing technique has become an effective tool for monitoring the oil spill risk in coastal areas. In this study, taking Jiaozhou Bay in China as the study area, an innovative oil spill monitoring framework was established based on Polarimetric SAR (PolSAR) images and deep learning theory. Specifically, a DeepLabv3+-based semantic segmentation model was trained using 35 Sentinel-1 satellite images of oil films on the sea surface from maritime sectors in different regions all over the world, which not only considered the information from the PolSAR images but also meteorological conditions; then, the well-trained framework was deployed to identify the oil films in the Sentinel-1 images of Jiaozhou Bay from 2017 to 2019. The experimental results show that the detection accuracies of the proposed oil spill detection model were higher than 0.95. It was found that the oil films in Jiaozhou Bay were mainly concentrated in the vicinity of the waterways and coastal port terminals, that the occurrence frequency of oil spills in Jiaozhou Bay decreased from 2017 to 2019, and that more than 80 percent of the oil spill events occurred at night, mainly coming from the illegal discharge of waste oil from ships. These data indicate that, in the future, the PolSAR technique will play a more important role in oil spill monitoring for Jiaozhou Bay due to its capability to capture images at night.

Impacts of climate events on life history parameters of major commercial fishes in the Beibu Gulf, South China Sea in the last 15 years

A detailed understanding of the impact of climate variability on fish life histories provides a fundamental basis for the ecosystem approach to fisheries management. In this study, we evaluated the relationship between fish life-history trait parameters and climate periods, using survey data from 2006−2020 for the Beibu Gulf of the northwestern South China Sea, a fishing ground with high commercial and ecological value. We show that climate events can significantly alter the structure of the life history for major commercial fishes, with more diverse life-history strategies during warm events and more concentrated mortality during cold events. Additionally, we detected special relationships between climate events and the life-history trait composite index (LTCI) inPsenopsis anomalaandTrichiurus lepturus. It is possible that other indicators would be more appropriate than variability in Oceanic Niño Index (ONI), such as variation in fishing effort or the environmental resistance of fish. Anthropogenic disturbance and biological features may also help explain the magnitude of population variability. We found that changes in life-history traits of only a few commercial fishes (Decapterus maruadsi,Pennahia macrocephalus, andUpeneus sulphureus) were related to the shift in climate periods; these fishes were generally characterized by faster growth and higher mortality rates in the normal and El Niño periods, with the opposite trends in La Niña periods. However, the emergence of climate events has led to a clear complementarity of life-history strategies among some fish in the same ecological niche (same genus and/or feeding group), which may be explained by a balance between habitat conditions and fishing pressures. This study of fish life-history strategies under climate anomalies provides key insights into important attributes for managers to consider when implementing relevant measures to promote fisheries sustainability in the subtropical bay.

Effects of solar energy development on ants in the Mojave Desert

AbstractLand‐use change from solar energy development may affect desert ecosystems and the soils, plants, and animals therein, yet our understanding of these interactions is nascent. With their ubiquity, criticality as ecosystem constituents, and sensitivity to environmental variation, ants may be useful study organisms for elucidating ecological effects of solar energy development in deserts. Our objectives were to disentangle the response of a desert ant community to solar energy development decisions and test the efficacy of ants as bioindicators at a solar power facility (392 MW) in the Mojave Desert, USA. We used pitfall traps to collect ants in treatments representing different solar energy development decisions, including variably intense site preparation practices: blading (i.e., bulldozing) and mowing, and establishment of undeveloped patches in solar fields, replicated across three power blocks comprising the facility and in undeveloped control sites surrounding the facility. We determined that ant abundance, species richness, Shannon Diversity Index, and functional richness were lower in bladed treatments than in all other treatments and controls. For most taxonomic and functional ant responses, we detected no difference between nonbladed treatments and controls; these results suggest that less intensive site preparation and increased spatial heterogeneity (i.e., undeveloped patches in solar fields) can reduce the negative effects of solar energy development on desert ants. However, our results indicate that ants may serve as useful bioindicators of the severity of anthropogenic disturbance from solar energy development in deserts, and indicator analysis signifies that solar energy infrastructure may negatively affect some species with high ecological value (e.g., harvester ants). Negative effects of solar energy development on ants can have significant implications for desert ecosystem function and integrity, but conservation‐minded solar facility design and construction may lead to avoidance of “bottom‐up” ecological ramifications of increased solar production during the renewable energy transition.

GIS Analysis of spatial-temporal variation of the ecological risk caused by element and organic pollutants in Lake Marmara (TURKIYE)

Located in western Anatolia (Turkey), Lake Marmara is a wetland with high ecological value. Lake Marmara, which was exposed to intense anthropogenic effects after 1940, is experiencing ecological deterioration in recent years. This study aimed to analyze the ecological risk level of the lake by using Enrichment Factor (EF), Toxic Risk Index (TRI), Modified Potential Ecological Risk Index (mER) etc. The research hypothesized that the discharge of water from the Gediz River and Kum Stream to the lake after 1940 triggered ecological risk problems. Moderate toxic risk, low potential ecological risk, moderate element contamination were detected throughout the lake. It was determined that Mo, P and Hg of anthropogenic origin and As, Ni, Cr, Cu of lithological origin created ecological risk at a regional scale. Domestic-industrial wastes and agriculture in the Gediz River basin and around the lake were identified as the dominant anthropogenic activity. The temporal variation of the ecological risk indices based on the elements did not reflect the anthropogenic traces of water discharge into the lake from the Gediz River basin. However, the temporal variation of TOC, N, P and CDP showed the effects of anthropogenic interventions in the lake. According to the threshold values; P is highly contaminated and N is close to the severely contaminated limit. The findings, while confirming the research hypothesis, showed that the dominant ecological risk factor in Lake Marmara is not elemental contamination, but organic pollutants, nitrogen and phosphate.

Influence of Natural, Climatic, and Industrial Factors on Air and Water Quality in The Kemerovo Region (Kuzbass, Russia)

Geographical and climatic features of the Kemerovo region (Kuzbass, Russia) determined the modern geological and geomorphological structure of the territory, as well as a characteristic pattern in the distribution of mineral deposits. The purpose of this work was to study the geomorphological and hydrological features of the Kemerovo region. Atmospheric circulation, relief, and vegetation are the climate's main factors. The combination of these factors forms the climatic characteristics of the region. Soils in open areas (meadows, hayfields) occupy a small area and have high economic and ecological value. Most of the forest is not affected by economic activities. The exception is disturbances in the territories affected by logging and mining enterprises, which especially affect the air and water quality in the region. All the obtained results of the assessment of the geomorphological and hydrological condition of the Kemerovo region should be used when planning further experiments on the stages of technical and biological reclamation of disturbed lands of mining enterprises to restore vegetation cover and the primary state of soils.

Heavy Metals in Surface Sediment of Plateau Lakes in Tibet, China: Occurrence, Risk Assessment, and Potential Sources.

Tibetan Plateau lakes have high ecological value and play a crucial role in maintaining ecological balance. This research aimed to study the pollution characteristics, ecological risk, and potential sources of eight heavy metals (As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) in the surface sediments of 12 Tibetan Plateau lakes. The results of the toxicity risk index (TRI) showed that only Gongzhu Tso (28.09) and La' ang Tso (20.25) had heavy metals that could pose a very high risk of toxicity to aquatic organisms. Hg posed the highest potential ecological risk to aquatic organisms. Based on the results of multiple analyses, we inferred that the contents of Cr, Cu, Hg, and Ni in sediments of Tibetan lakes were influenced by industrial and agricultural development; Cd, Pb, and Zn were influenced by transport and atmospheric transport; and As was derived from geothermal activity and rock weathering.

Chromosome-level genome assembly of Quercus variabilis provides insights into the molecular mechanism of cork thickness

Quercus variabilis is a deciduous woody species with high ecological and economic value, and is a major source of cork in East Asia. Cork from thick softwood sheets have higher commercial value than those from thin sheets. It is extremely difficult to genetically improve Q. variabilis to produce high quality softwood due to the lack of genomic information. Here, we present a high-quality chromosomal genome assembly for Q. variabilis with length of 791,89 Mb and 54,606 predicted genes. Comparative analysis of protein sequences of Q. variabilis with 11 other species revealed that specific and expanded gene families were significantly enriched in the "fatty acid biosynthesis" pathway in Q. variabilis, which may contribute to the formation of its unique cork. Based on weighted correlation network analysis of time-course (i.e., five important developmental ages) gene expression data in thick-cork versus thin-cork genotypes of Q. variabilis, we identified one co-expression gene module associated with the thick-cork trait. Within this co-expression gene module, 10 hub genes were associated with suberin biosynthesis. Furthermore, we identified a total of 198 suberin biosynthesis-related new candidate genes that were up-regulated in trees with a thick cork layer relative to those with a thin cork layer. Also, we found that some genes related to cell expansion and cell division were highly expressed in trees with a thick cork layer. Collectively, our results revealed that two metabolic pathways (i.e., suberin biosynthesis, fatty acid biosynthesis), along with other genes involved in cell expansion, cell division, and transcriptional regulation, were associated with the thick-cork trait in Q. variabilis, providing insights into the molecular basis of cork development and knowledge for informing genetic improvement of cork thickness in Q. variabilis and closely related species.

Chronicle of a death foretold: The vanishing of an emblematic cultural landscape results in the loss of its unique plant communities

Badlands are peculiar geomorphological formations shaping landscapes of high ecological and cultural value. In the last decades, land reclamation for agricultural purposes and the cessation of traditional land use, such as sheep grazing, led to their decline in extent in many areas. To quantify the changes in badland-related plant communities, we resurveyed badland vegetation in a site of the Crete Senesi (Siena, central Italy) after 16 years (2006–2022), using 48 quasi-permanent vegetation plots and by means of uni- and multivariate analysis of variance. We found an increase in the total vegetation cover of plant communities growing in former bare soil and sparsely vegetated areas, in line with an overall decrease in the extent of bare soil surfaces in the study area, which we highlighted through the analysis of multitemporal satellite images. Pioneer vegetation characterized by the endemic plant Artemisia caerulescens subsp. cretacea changed into ruderal annual grasslands, while former bare soils were colonized by such pioneer vegetation. In contrast, perennial grasslands remained stable. Grasslands with shrubs became more similar to perennial grasslands in species composition. Species richness increased in former bare soils, and using the total vegetation cover as a proxy for successional stages, we found that Shannon diversity and evenness peaked at about 90% of total cover. In all the stages of colonization, short distance dispersal species prevailed, both therophytes (Avena sterilis, Parapholis strigosa) and perennials (Artemisia caerulescens subsp. cretacea, Bromopsis erecta). Long distance dispersal species (Galatella linosyris) started colonizing at about 60% of total vegetation cover, and at high vegetation cover all the functional groups coexisted. Our results confirm that the badland landscapes of southern Tuscany and specialist plant diversity adapted to badlands are vanishing after a diminishing of active land management, suggesting the current ineffectiveness of the Natura 2000 network in their conservation.

Exploring the Ecological Benefits of Dead Wood and the Opportunities of Interpreting Dead Wood to the Public

According to the Practice Guide of Managing deadwood in forests and woodlands, all types of dead and dying trees of 10 cm or more in diameter are recognised as deadwood. Dead wood is very important to the health of the forest, and this is increasingly recognized by environmentalists and ecologists. Dead wood is thought to be vital to the health of a wood or forest because it can help to reduce soil erosion and create stability (Gamekeeperstrust, 2019). Not only is it an aspect of the nutrient cycling process, it provides a stable, slow-release nitrogen source and is thought to play an important role in carbon storage. Falling logs can also increase the soil stability of the woodland (Pupplet, n.d.). Although every forest and woodland are different, and owners and managers have different management objectives, deadwood should be considered in most situations. Current evidence suggests that, over the long term, deadwood should amount to roughly 20 m3 per hectare averaged across the forest management unit. Some management actions are general to all woodlands, but there are others which are specific to woods or areas of higher ecological value. This approach requires that areas of high ecological value be identified during management planning. The public as the target users of our future design, their attitude is also essential to integrate the characteristics of dead wood into the design of urban environment landscape.

Identification and geomorphic characterization of fluvial knickzones in bedrock rivers from Courel Mountains Geopark

The gradient characteristics of Courel Mountains Geopark bedrock rivers were examined. Unlike alluvial rivers, bedrock rivers have been the great forgotten of fluvial geomorphology globally. Based on the decreasing rate of gradient with increasing measurement length, a relative steepness was obtained as indicator of knickzone. Supported by GIS techniques and DEMs, the changes in slope along the longitudinal profile of the rivers were detected. The number of the extracted knickzones rises to 325, which means a frequency of knickzones of 0.467 km−1. The total length of the knickzones is 285 km, representing about half of the drainage network as knickzone (47%). The mean height, the length, and the gradient of all the knickzones were ~ 110 m, ~ 880 m, and 0.178 m·m−1, respectively. There is no distribution pattern and the knickzones are everywhere, although they are more present in reaches with NW–SE direction and order 1. Several environmental factors were crossed to know more about the occurrence and knickzones characteristics, suggesting that density and direction of fractures regulate the number and the trajectory of the knickzones, while the lithology controls the singularity of the forms. The geomorphological and the topographical characteristics of the bedrock rivers make them high ecological, scenic, landscape, and recreational value. Findings from this study can be also used by managers to develop and/or improve strategies for conservation, valorisation, and how to approach the tourist who visits the Geopark. Scientific tourism can offer a unique and educational travel experience, allowing participants to learn about bedrock rivers and knickzones.

Comparative and population genomics of buckwheat species reveal key determinants of flavor and fertility

Common buckwheat (Fagopyrum esculentum) is an ancient crop with a world-wide distribution. Due to its excellent nutritional quality and high economic and ecological value, common buckwheat is becoming increasingly important throughout the world. The availability of a high-quality reference genome sequence and population genomic data will accelerate the breeding of common buckwheat, but the high heterozygosity due to the outcrossing nature has greatly hindered the genome assembly. Here we report the assembly of a chromosome-scale high-quality reference genome of F. esculentum var. homotropicum, a homozygous self-pollinating variant of common buckwheat. Comparative genomics revealed that two cultivated buckwheat species, common buckwheat (F. esculentum) and Tartary buckwheat (F. tataricum), underwent metabolomic divergence and ecotype differentiation. The expansion of several gene families in common buckwheat, including FhFAR genes, is associated with its wider distribution than Tartary buckwheat. Copy number variation of genes involved in the metabolism of flavonoids is associated with the difference of rutin content between common and Tartary buckwheat. Furthermore, we present a comprehensive atlas of genomic variation based on whole-genome resequencing of 572 accessions of common buckwheat. Population and evolutionary genomics reveal genetic variation associated with environmental adaptability and floral development between Chinese and non-Chinese cultivated groups. Genome-wide association analyses of multi-year agronomic traits with the content of flavonoids revealed that Fh05G014970 is a potential major regulator of flowering period, a key agronomic trait controlling the yield of outcrossing crops, and that Fh06G015130 is a crucial gene underlying flavor-associated flavonoids. Intriguingly, we found that the gene translocation and sequence variation of FhS-ELF3 contribute to the homomorphic self-compatibility of common buckwheat. Collectively, our results elucidate the genetic basis of speciation, ecological adaptation, fertility, and unique flavor of common buckwheat, and provide new resources for future genomics-assisted breeding of this economically important crop.

Ectomycorrhizal community associated with Cedrus deodara in four urban forests of Nantong in East China.

Ectomycorrhizal (ECM) fungi play fundamental roles in host plant growth and terrestrial ecosystems. Cedrus deodara is cultivated in several regions in China, has high ecological, economic and medicinal value, for its afforestation and providing timber and wood oil. Here, we investigated ECM colonization status of four urban C. deodara forests in Nantong, East China. We also characterized soil spore banks by conducting bioassay experiments using soils collected from these forests. In total, we identified 19 ECM fungal species, of which 13 species were found in mature forests and 9 species were identified in bioassay experiments, with only 3 species shared. Soil pH and available P content had significant effects on species occurrence in both mature trees and bioassay seedlings on local scales. ECM communities clearly (A = 0.391, p = 0.006) separated mature forests from spore banks. Thelephoracae was the richest family we detected associated with C. deodara, while Trichophaea sp. was the most dominant in mature forests, and Wilcoxina sp. was dominant in spore banks. ECM richness affected the growth of bioassay seedlings, especially after inoculation with 2 ECM species, promoting root growth, significantly (F = 3.028, p = 0.050), but it had no effects on shoots (F = 1.778, p = 0.177). No effect of inoculation rate was found on seedlings growth. To conserve this important tree species, the ECM fungi that are associated with it should be considered.

Constructing an urban cooling network based on PLUS model: Implications for future urban planning

Many studies on mitigation of the surface urban heat island (SUHI) are conducted from the perspective of landscape patch allocation, and few studies have investigated the overall impact of network connectivity on SUHI. In this study, an ecological network framework is proposed to identify the key patches and cooling corridors. Based on surface temperature inversion data, morphological spatial pattern analysis (MSPA) and landscape connectivity indexes are used to identify heat island and cold island patches with high ecological value on which to construct the network. The innovative patch-generating land use simulation (PLUS) model is introduced to construct a resistance surface, and Circuit theory (CT) is used to connect cold islands and heat islands in cooling corridors. The results show that from 1999 to 2019, land surface temperature (LST) increased significantly in the city of Haikou, the extreme LST became more and more significant, and the area with the highest temperatures showed a southward trend. The connectivity between cold and heat island patches declined, and the Loop, Bridge and Branch areas in heat islands increased. The latter scenario is conducive to the construction of cooling corridors. The land cover index had a great influence on SUHI, and the resistance surface presented a distribution pattern with high values in the northwest and low values in the southeast, which is consistent with the distribution of LST. Finally, we extracted the resultant cold-heat island cooling network, which was mainly concentrated along the southern edge of the study area, and found that corridor construction faced fewer obstacles in 2019 than in 1999. This study can guide urban planners to reinforce the parts of ecological networks that will mitigate the urban heat island effect.

Overview of Urban Landscape Rewilding Research

Urban wildscape refers to landscape units within or surrounding urban areas that are primarily shaped by natural processes exhibiting high ecological value and diversity. Due to urbanization urban wildscapes are at risk of disappearing or degrading but they also present opportunities for the protection and restoration of urban ecosystems. The purpose of this article is to systematically review and analyze the concepts classifications values threats and conservation strategies of urban wildscapes in order to provide references for urban planning and wildscape design.

Integrated physiological and metabolomic analyses reveal changes during the natural senescence of Quercus mongolica leaves.

Quercus mongolica is a common landscape, afforestation, and construction timber species in northern China with high ecological, economic, and ornamental value. Leaf senescence is a complex process that has important implications for plant growth and development. To explore changes of metabolites during the ageing of Quercus mongolica leaves, we investigated physiological responses and metabolite composition in ageing leaves harvested from 15-20-year-old Quercus mongolica. Leaf samples of Q. mongolica were collected when they were still green (at maturity) (stage 1), during early senescence (stage 2), and during late senescence (stage 3). These leaves were then subjected to physiological index and metabolome sequencing analyses. The physiological analysis showed that the leaves of Q. mongolica changed from green to yellow during senescence, which induced significant accumulation of soluble sugar and significant reductions in the concentration of soluble protein and chlorophyll. Peroxidase and catalase were the main antioxidant enzymes mitigating leaf senescence. Metabolomic analysis identified 797 metabolites during leaf senescence. Compared to stage 1, 70 differential metabolites were screened in stage 2 and 72 were screened in stage 3. Differential metabolites in the two senescent stages were principally enriched in amino acid metabolism, lipid metabolism and secondary metabolite biosynthesis. The contents of N-oleoylethanolamine and N, N-dimethylglycine were significantly increased only in stage 2, while the contents of trifolin, astragalin, valine, isoleucine, leucine, and citric acid were significantly increased only in stage 3. Histidine, homoserine, tryptophan, tyrosine, phenylalanine, proline, norleucine, N-glycyl-L-leucine, linoleic acid, linolenic acid, gallic acid, 3-indoleacrylic acid, 3-amino-2-naphthoic acid, 3-hydroxy-3-methylpentane-1,5-dioic acid, 2,3,4-trihydroxybenzoic acid, trifolin, astragalin, DL-2-aminoadipic acid, pinoresinol dimethyl ether, dimethylmatairesinol, and lysophosphatidylcholine increased during both stage 2 and stage 3. Increasing contents of these metabolites may constitute the main mechanism by which Q. mongolica leaves adapt to senescence.

Spatial dynamics in the urban-rural-natural interface within a social-ecological hotspot

City-regions in sensitive environmental spaces such as globally recognized biodiversity hotspots face the challenge of adopting land use planning strategies that facilitate the transition towards sustainability. Though landscape-scale patterns of land use changes have already been documented in central Chile, a critical spatial assessment of the dynamics and forces shaping current land use and the conservation landscape in the Valparaíso-Santiago city-region has not yet been undertaken. We applied a satellite-based survey of urban sprawl and the extension and recurrence of wildfires spanning a 35-year timeframe from 1985 to 2020. We compared their spatial occurrence with high ecological value areas (HEVA) and analyzed the resulting patterns within the framework of an ad-hoc centrifugal forces model. Results show a constant expansion of urban sprawl and direct impacts of wildfires affecting biodiversity values. La Campana-Peñuelas Biosphere Reserve emerges as a relevant spatial feature that can facilitate the transition towards sustainability at the regional scale, although the laissez-faire stance of the dominant neoliberal economic model in Chile is the most evident threat to the transition toward sustainability. We conclude that the study area, with the attributes of a social-ecological hotspot, shows strengths such as a space capable of transformative change and the potential of a regenerative city-region.

High vulnerability of coastal wetlands in Chile at multiple scales derived from climate change, urbanization, and exotic forest plantations

Coastal wetlands are considered one of the most vulnerable ecosystems worldwide; the ecosystem services they provide and the conservation of their biodiversity are threatened. Despite the high ecological and socioenvironmental value of coastal wetlands, regional and national vulnerability assessments are scarce. In this study we aimed to assess the vulnerability of coastal wetlands in Chile from 18°S to 42°S (n = 757) under a multiscale approach that included drivers associated with climate change and land cover change. We assessed multiple drivers of vulnerability at three spatial scales (10 m, 100 m, and 500 m) by analyzing multiple remote sensing data (16 variables) on land cover change, wildfires, climatic variables, vegetation functional properties, water surface and importance for biodiversity. We constructed a multifactorial vulnerability index based on the variables analyzed, which provided a map of coastal wetland vulnerability. Then we explored the main drivers associated with the vulnerability of each coastal wetland by performing a Principal Components Analysis with Agglomerative Hierarchical Clustering, which allowed us to group coastal wetlands according to the drivers analyzed. We found that 42.6 ± 9.2 % of the coastal wetlands evaluated have high or very high vulnerability, with higher vulnerability at the 500 m scale (51.4 %). We identified four groups of coastal wetlands: two located in central Chile, mainly affected by climate change-associated drivers (41.9 ± 2.1 %), and one in central Chile which is affected by land cover change (52.8 ± 6.2 %); the latter has a lower vulnerability level. The most vulnerable coastal wetlands were located in central Chile. Our results present novel findings about the current vulnerability of coastal wetlands, which could be validated by governmental institutions in field campaigns. Finally, we believe that our methodological approach could be useful to generate similar assessments in other world zones.

Facemasks: An insight into their abundance in wetlands, degradation, and potential ecotoxicity

Disposable facemasks represent a new form of environmental contamination worldwide. This study aimed at addressing the abundance of facemasks in an overlooked natural environment with high ecological and economic value – the wetlands (Ria de Aveiro, Portugal, as study case), evaluating their potential biodegradation using naturally occurring fungi and assessing the potential ecotoxicity of released microfibres on local bivalves.All masks collected within 6500 m2 area of Aveiro wetland were 100 % disposable ones (PP-based, confirmed by Fourier transform infrared spectroscopy - FTIR) with an initial abundance of 0.0023 items/m2 in Sept. 2021, which was reduced by ∼40 % in Apr. 2022 and ∼87 % in Sept. 2022, as a reflection of the government policies. Analysis of the carbonyl index (0.03 to 1.79) underlined their state of degradation, primarily due to sun exposure during low tides. In laboratory conditions, 1 mm2 microplastics obtained from new disposable facemasks were prone to biodegradation by Penicillium brevicompactum and Zalerion maritimum inferred from microplastics mass loss (∼22 to −26 % and ∼40 to 50 %, respectively) and FTIR spectra (particularly in the hydroxyl and carbonyl groups). In addition, microfibres released from facemasks induced sublethal effects on the clam, Venerupis corrugata, mostly in their UV-aged form when compared to pristine ones, characterised by a decrease in cellular energy allocation (CEA) and an increase in aerobic energy metabolism (ETS). Concomitantly, clams exposed to 1250 items/L of UV-aged microplastics (similar to field-reported concentrations) expressed greater clearance capacity, indicating a need to compensate for the potential energy unbalance.This study provides the first baseline monitoring of facemasks in wetlands while bringing new evidence on their biodegradation and ecotoxicity, considering environmentally relevant conditions and keystone organisms in such environments. Such studies require scientific attention for rapid regulatory action against this emerging and persistent pollutant, also targeting remediation and mitigation strategies considering these items under pandemic scenarios.