High Levels Of Carbohydrates Research Articles

Characteristics of Composite Flour Made of Kidney Bean and Soybean

Kidney beans and soybeans have the potential to be developed because they contain good nutrient. Kidney beans and soybeans are sources of vegetable protein. One form of development of legume products is composite flour. The aim of this study was to analyze the physical and chemical characteristics of composite flour made of kidney beans and soybeans. The experiment was designed using Completely Randomized Design with nine treatments and each treatment was replicated three times. The factor investigated was formulation of composite flour made from kidney bean and soybean (90%:10%; 80%:20%; 70%:30%; 60%:40%; 50%:50%; 40%:60%; 30%:70%; 20%:80%; and 10%:90%). The swelling power, solubility, color, white degree, moisture content, ash, protein, fat, and carbohydrate content were analyzed. The results showed that the higher percentage of soybean flour caused a decrease on swelling power, white degree, and redness of composite flour, but it increased the value of solubility, lightness and yellowness of composite flour. Composite flour with higher percentage of kidney bean flour had higher carbohydrate level, while composite flour with higher percentage of soybean flour had higher protein and fat content. The moisture contents of composite flour made of kidney bean and soybean were in accordance with the standard for beans flour products.

The positive effects of dietary arginine on juvenile hybrid snakehead (Channa maculata♀×Channa argus♂) fed high-carbohydrate diets: Liver inflammation antioxidant response, and glucose metabolism

The aim of this study was to investigate the effects of arginine supplementation on liver morphology, antioxidant capacity, inflammatory response, insulin signaling pathway and glucose metabolism in hybrid snakehead. Five experimental diets (2.31 %, 2.42 %, 2.72 %, 2.92 %, and 3.12 % arginine) were prepared by adding different levels of arginine to the basal diet containing high (27 %) level of carbohydrate. The results showed that the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione peroxidase (GSH-Px) of fish fed with arginine diets were significantly higher than those in control group (p < 0.05), accompanied by the lower malondialdehyde (MDA) content in the same groups (p < 0.05). There were obvious mild inflammatory cell infiltration and increased perihepatic pancreatic space in control group, while the pathological state of the liver was relieved in fish fed with arginine diets. Dietary arginine supplementation resulted in significantly higher glucose metabolism-related genes glucokinase (GK), phosphoenolpyruvate carboxkinase (PEPCK), glycogen synthase (GS) and pyruvate kinase (PK) and insulin signaling pathway key genes insulin receptor subset-1 (IRS-1), protein kinase B (Akt) and phosphoenolpyruvate carboxykinase (PI3K)(p < 0.05). Immune-related genes transforming growth factor β (TGF-β), heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90) were significantly higher in the arginine supplemented diet than in the control group (p < 0.05). Immune-related genes interleukin-8 (IL8), interleukin-1β (IL1β) and tissue tumor necrosis factor-α (TNF-α) in fish fed with arginine diets were significantly lower than those in control group (p < 0.05). In conclusion, dietary arginine can alleviate the adverse effects of high carbohydrate diet on hybrid snakeheads by improving glucose metabolism and enhancing antioxidant, anti-inflammatory and non-specific immune functions.

Formulating finger millet (Eleusine coracana L) and cowpea (Vigna unguiculata) food blend for enhanced micronutrients for children below 5 years

Malnutrition of children under 5 years is a public health concern because it is the most vulnerable stage of child’s growth where its impact results in long term health conditions such as stunting, wasting, obesity and poor cognitive development. This study aimed at developing a Rapoko-Cowpea porridge blend to enhance dietary micronutrients among children below the age of 5 years and assess its sensory acceptability. A mixed methods research approach was adopted consisting of a quasi- experiment and a cross sectional survey. Vendors from Mbare market were clustered based on the source of their finger millet and cowpea grains (Masvingo, Murewa, Gokwe). A Completely Randomised Design (CRD) was used with three treatments (blends) replicated three times. Proximate analysis was done using standard AOAC methods. Compared to cowpea, finger millet had the highest levels of carbohydrates (78.12g/100g), iron (8.7mg/100g), zinc (3.77mg/100g). Results also show that 75:25. Rapoko-Cowpea blend ratio had the ability to meet WHO daily nutrient requirements for children under the age of 5 years. Blend ratio of Rapoko: cowpeas (75:25) had the highest carbohydrates (69.41g/100g), protein (25.64g/100g), zinc (1.74mg/100g), iron (5.14mg/100g), energy (411.37kcal), and vitamins (0.11 mcg RAE) with RaCoB1 blend having the lowest nutrient composition. Results have showed that finger millet (Rapoko), cowpeas and RaCoB (Rapoko-Cowpea blend) blends have high macro and micronutrients (iron and zinc) to support daily nutritional requirements of children below the age of 5 years and may help in addressing malnutrition. Sensory evaluation was done with 100 panellists from the University of Zimbabwe and of the three blends, a 75:25 ratio was the most preferred (90.88%). Further studies need to analyse the effects of anti-nutritional factors, bioavailability of nutrients, shelf life of the food blend and commercialization potential of RaCoB blends. The government is therefore encouraged to provide agronomic support for increased productivity of small grains and pulses through improved research and extension. There is a need to develop a policy at national level that promotes blending of cereals and pulses as well as innovative small scale food processing initiatives through private-public partnerships. Key words: Children under 5 years, Cowpea, food blending, Malnutrition, Micronutrients, Rapoko, Sensory evaluation

Effect of co-inoculation of Lactobacillus plantarum and Lentilactobacillus buchneri on aerobic stability and microbial community composition of perennial sorghum silage

In the present study, the aerobic stability of perennial sorghum (Sorghum bicolor × Sorghum propinquum) silage following exposure to air was evaluated, which was pretreated with either Lactiplantibacillus plantarum (L group) or Lactiplantibacillus plantarum + Lentilactobacillus buchneri (M group). Samples from the M group had lower dry matter loss rates and higher acetic acid content after exposure to air. Furthermore, samples from the M group showed high levels of water-soluble carbohydrates (2.79–6.55% in dry matter) and crude protein (3.82–9.02% in dry matter) after exposure to air. Moreover, M pretreatment led to the inhibition of the growth of Debaryomyces hansenii whereas it promoted the growth of Levilactobacillus and Clavispora sp. Overall, M pretreatment of perennial sorghum during storage hindered aerobic deterioration and promoted the formation of total fermentation products that act as precursors for biogas production.

Quality Evaluation of Noodles Produced From Blends of Wheat, Unripe Banana and Cowpea Flours

The work determined the quality of noodles produced from wheat, unripe banana and cowpea flour blends. Flour blends were produced from wheat, cowpea and unripe banana flour at the ratios of 100:0:0, 80:10:10, 70:10:20, 60:10:30, 50:10:40 (wheat: unripe banana: cowpea). Noodles were prepared from the flour blends and evaluated for the chemical composition, color, cooking characteristics and sensory properties. Noodles from 100% wheat flour served as control. The cowpea flour contained higher amount of protein while the unripe banana flour and wheat flour had higher levels of carbohydrate than the cowpea flour. The wheat flour had higher sodium, calcium, magnesium and phosphorous contents than the other flours, which were closely followed by those of the unripe banana flour. The moisture contents of the noodles varied from 9.15-11.25%. The ash contents of the noodles ranged between 1.03 and 1.80%.The crude fiber, fat and carbohydrate contents of the noodles increased with the level of cowpea flour in the noodles. The energy contents of the noodles ranged from 383.39-422.31Kcal/100g. The cooking time decreased from 10.50 min in wheat flour noodles to 7.50 min in the noodle containing 40% cowpea flour. However, the cooking loss and weight increased as the amount of cowpea flour in the noodles increased. The wheat flour noodles were rated higher for color, taste and overall acceptability but not for flavor and mouthfeel. The score for overall acceptability of the wheat flour noodle was 8.60 while those of the composite flour noodles containing 10 and 20% cowpea flour were 8.15 respectively on 9-point Hedonic scale. It is concluded that noodles could be produced from 70% wheat, 20% cowpea and 10% unripe banana flour blends without affecting the qualities of the noodles.

Neotropical Frog Foam Nest's Microbiomes.

Amphibian foam nests are unique microenvironments that play a crucial role in the development of tadpoles. They contain high levels of proteins and carbohydrates, yet little is known about the impact of their microbiomes on tadpole health. This study provides a first characterization of the microbiome of foam nests from three species of Leptodactylids (Adenomera hylaedactyla, Leptodactylus vastus, and Physalaemus cuvieri) by investigating the DNA extracted from foam nests, adult tissues, soil, and water samples, analyzed via 16S rRNA gene amplicon sequencing to gain insight into the factors driving its composition. The results showed that the dominant phyla were proteobacteria, bacteroidetes, and firmicutes, with the most abundant genera being Pseudomonas, Sphingobacterium, and Paenibacillus. The foam nest microbiomes of A. hylaedactyla and P. cuvieri were more similar to each other than to that of L. vastus, despite their phylogenetic distance. The foam nests demonstrated a distinct microbiome that clustered together and separated from the microbiomes of the environment and adult tissue samples. This suggests that the peculiar foam nest composition shapes its microbiome, rather than vertical or horizontal transference forces. We expanded this knowledge into amphibian foam nest microbiomes, highlighting the importance of preserving healthy foam nests for amphibian conservation.

The Effect of Concentration of Skipjack Tuna (Katsuwonus pelamis) Powder and Drying Time on The Chemical Characteristics of Purple Sweet Potato (Ipomoea batatas L) Tortilla Chips

The Effect of Concentration of Skipjack Tuna (Katsuwonus pelamis) Powder and Drying Time on The Chemical Characteristics of Purple Sweet Potato (Ipomoea batatas L) Tortilla Chips

Yetiştiriciği Yapılan Üç Tür Alabalığın; Kaynak Alabalığı (Salvelinus fontinalis, Mitchill 1814), Gökkuşağı Alabalığı (Oncorhynchus mykiss Walbaum, 1972) ve Karadeniz Alasının (Salmo trutta labrax Pallas 1811) Biyokimyasal Özelliklerinin Farklılıkları

The objective of this present study is to signify biochemical features of three reared trouts (brook trout, rainbow trout, Black Sea trout) that are economically consequential and reared fish species in the Eastern Black Sea region. The rainbow trout has been reared successfully for a long period of time. However, brook trout and the Black Sea trout have been two new species to be reared in the region with a high potential contribution to the economy. Therefore, there is a strong need to determine and report the differences between the fish (especially the two new species), levels of carbohydrates, energy, carotenes (Vitamin A), fatty acid, and proximate composition (protein, lipid, moisture, and ash). In addition, the lipid indices [Atherogenic Index (AI), Thrombogenic Index (TI), and polyene index (PI)] were also measured and reported for the trouts. Moreover, the color of the fillets of the fish was also measured. The results of this present study show that the average levels of moisture and protein varied while the average levels of lipid and ash were close to each other. The highest levels of carbohydrates, energy, and carotenes were determined in the muscle of brook trout in this study. The highest meat yield was also obtained from brook trout followed by Black Sea trout and rainbow trout. A total of 19 fatty acids were determined for Black Sea trout and brook trout and 17 fatty acids for rainbow trout in the present study. The omega-3 levels of the all trout used for the present study were roughly twice as much as that of the omega-6, except for rainbow trout.

Glycemic Responses of Milk and Plant-Based Drinks: Food Matrix Effects.

The consumption of food items containing digestible carbohydrates in food products leads to postprandial increases in blood glucose levels and glycemic responses. The extent to which these occur depends on many factors, including concentration and type of carbohydrate, but also other physicochemical properties of the food matrix, which determine the rate of uptake of monosaccharides into the bloodstream, including product structure and factors affecting gastric emptying. For milk, control of postprandial glycemic responses appears to be multifaceted, including a controlled rate of gastric emptying, a rate of glucose and galactose uptake into the bloodstream controlled by enzymatic hydrolysis, as well as stimulated insulin secretion to enhance uptake of blood glucose from the bloodstream. Altogether, this allows milk to deliver comparatively high levels of carbohydrate with limited glycemic responses. For plant-based drinks positioned as milk alternatives, however, compositional differences (including carbohydrate type and concentration) as well as matrix factors limiting control over gastric emptying and insulin secretion can, in some cases, lead to much stronger glycemic responses, which are undesirable in relation to non-communicable diseases, such as type-2 diabetes. This review discusses glycemic responses to milk and plant-based drinks from this perspective, focusing on mechanistic insights and food matrix effects.

Multi-Nutrient Analysis of Dietary Macronutrients with All-Cause, Cardiovascular, and Cancer Mortality: Data from NHANES 1999-2014.

Macronutrients are a major component of the human diet. However, few studies have assessed their collective association with mortality. We sought to evaluate the associations of macronutrient intake with all-cause, cardiovascular, and cancer mortality in US adults using a multi-nutrient approach. This prospective cohort analysis used data from the National Health and Nutrition Examination Survey from the years 1999 to 2014. The participants included 33,681 US adults aged 20-85 years (52.5% female). The maximum follow-up time was 16.8 years, with a total of 4398 total deaths, including 772 cardiovascular deaths and 952 cancer deaths. The associations between mortality and dietary macronutrients were explored using three-dimensional generalized additive models, allowing for visual and statistical inference of complex nonlinear associations. Absolute macronutrient intake demonstrated a three-way interactive association with all-cause mortality (p &lt; 0.001), cardiovascular mortality (p = 0.02), and cancer mortality (p = 0.05), adjusted for age, sex, ethnicity, socioeconomic status, dietary quality, and lifestyle. Compositionally, a high caloric diet composed of moderately high protein (20%), moderate fat (30%), and moderate carbohydrate (50%) levels was associated with the highest mortality risk. Across the total energy intake levels, lower mortality risk was observed in two separate regions consisting of higher protein (30%), higher carbohydrate (60%), and lower fat levels (10%) or lower protein (10%), moderate carbohydrate (45%), and higher fat levels (45%). These findings highlight a complex nonlinear and interactive association between macronutrients and all-cause mortality such that several distinct dietary compositions are associated with similarly high or low risk. Future research is needed to explore the drivers of these associations and whether they differ across varying dietary patterns and populations.

Comparative study on nutritional value of Macrobrachium rosenbergii and Macrobrachium malcolmsonii

The purpose of the present study is to estimate the nutritional parameters such as protein, carbohydrate, lipid, moisture and ash content in two different fresh water prawns. The proximate composition was estimated in five different samples from each species and the values were tabulated as Mean±SD. For the proximate analysis in the present study the samples were procured from cultured and frozen conditions. It is evident from the present study findings that the highest average protein content was recorded in cultured conditions than frozen conditions. Similarly the highest lipid content was recorded in cultured conditions than frozen conditions. The highest levels of carbohydrates were seen in frozen conditions than cultured conditions.

Characterization and potential use of olive pies from different extractions and industries in ruminant feeding

This work aimed to characterize coproducts from extra virgin olive oil extraction, its chemical composition and nutritive value of two olive cultivars (Arbequina and Koroneiki) in industries in Brazil Southern region. Sampling from cultivar Arbequina was done at two moments (time 0 and 48h post processing) and from cultivar Koroneiki, only at time zero was sampled. The composition of the two cultivars was different, as expected. Higher levels of carbohydrates (CHO) and lower levels of neutral detergent fiber (NDF) contributed to improve in situ degradability (61.0 vs 43.5% ) of Koroneiki cultivar. Both co-products obtained on the day of processing showed high levels of lignin, which led to a lower level of in situ degradability of the NDF (21.1%). High levels of EE (19.4% DM), total phenols (FT; 1.8% DM), total tannins (TT; 1.11% DM) and TDN (72.7% DM) were also observed. These results demonstrate that the byproduct represents a potential source of lipids and phenolic compounds that can be used to adjust diets as well as the aggregation of functional molecules in meat and milk products. The care in obtaining and preserving the product is important because the material obtained 48 hours after the processing showed losses in the contents of CHO, CNF, FT and TT, which can impair its nutritional value and the potential of use.

Valorização do resíduo casca da fruta lichia para a produção sustentável de um ingrediente de valor agregado

Abstract Lychee (Litchi chinensis Sonn.) is an exotic fruit from Asia, recently introduced in Europe. With the increase in the world production of this fruit, many by-products are wasted during industrial processing, including their peels and seeds. Considering the utilization of fruit peels as edible material as a new functional ingredient, this work aimed to assess the nutritional and phytochemical characterization, as well as the antioxidant activity of two lychee peel extracts (alcoholic and hydroalcoholic). Also, it was evaluated the scavenging capacity against reactive species (O2•-, H2O2, NO•) of the two extracts. The peels, at a nutritional level, showed high levels of carbohydrates and total energy (76.8 ± 1.0 g/100 g and 331.4 kcal/100 g, respectively) and low protein and lipid content. Total phenolic and flavonoid contents were higher in alcoholic extraction (1578 mg GAE/g and 55.1 mg CE/g, respectively). The antioxidant activity evaluated in vitro by DPPH and FRAP assays was also higher in the ethanolic extract, verifying a positive correlation with the extractive yield of the bioactive compounds. In general, the ethanolic extracts of lychee peels showed higher antioxidant capacity and the maximum scavenging activity against reactive oxygen (O2-•) and nitrogen species (NO•). The hydrogen peroxide scavenging activity observed in ethanolic extract (64 µg/mL) was like the values obtained in the positive controls (quercetin and ascorbic acid, 62 µg/mL, and 46 µg/mL, respectively). These preliminary results suggest this undervalued ingredient is a promising source of bioactive compounds with high biological potential for the development of new products as functional ingredient, always focusing on sustainability.

Determination of the Application Parameters of Spraying Drones for Crop Production in Hazelnut Orchards

Hazelnut, which has the most common cultivation area after almonds and walnuts among the shell fruits in the world, contains high levels of fat, protein, carbohydrates, various minerals and vitamins. Hazelnut has a broad growing area around the world, and Turkey accounts for 58% of world production with 776,000 tons annually. With this production capacity, Turkey ranks first in hazelnut production over the world. Harmful insects in hazelnut trees are the main factors that reduce hazelnut yield and quality. Pesticides are sprayed with backpack sprayers in the fight against these pests in hazelnut trees. Farmers are directly exposed to pesticides in these practices, which use high amounts of pesticides and water. In recent years, the use of unmanned agricultural vehicles in agriculture has increased. Drones are also used in pesticide applications in agriculture. In this study, the suitability of pesticide applications with the drone in hazelnut fields in Giresun province in terms of field conditions and spraying efficiency was investigated. In September 2021, applications were made with DJI Agras MG-1P model spraying drone in a selected hazelnut orchard in Uzgur village of Giresun province. The drone has 4 Teejet XR11001VS fan jet nozzles. Water-sensitive papers were placed on different regions on the hazelnut trees to be sprayed for drop measurements. In the experiments, hazelnut trees were sprayed using water at different heights and spraying rates. 6 flights were carried out at 1.5 and 2 meters altitudes and 1, 2 and 3 L.da-1 spray rates, and three hazel trees were sprayed as three repetitions in each flight. After the flight trials, the water-sensitive papers were scanned on the scanner, and the volume median diameters and the number of droplets per square centimetre were calculated in the DepositScan software. As a result of the analyses done, it was observed that the applications performed at 1 L.da-1 and 2 L.da-1 spray rates would not be sufficient in terms of spraying efficiency. As the drone flight altitude increased, the accumulation of the drops on the inner leaves decreased. In terms of drop distribution, the most homogeneous application parameter was found to be 1.5 meters high above the upper leaves of the hazel trees and 3 L.da-1 spray rate.

High carbohydrate increases amylase, plasma glucose, and gene expression related to glycolysis in giant gourami Osphronemus goramy.

The present study elucidated hepatic molecular and physiological responses of giant gourami to high dietary carbohydrates. Two levels of dietary carbohydrate, normal carbohydrate (34%, NC) and high carbohydrate (53%, HC), were offered to the fish for 60days. We evaluated the expression of genes that are related to carbohydrate metabolism, lipogenic capacities, amino acid catabolism, Krebs cycle, and energy sensing. In addition, we also observed the digestive enzyme activities, plasma glucose, glycogen content, whole-body composition, and growth performance of the fish. On day 30 after treatment, fish fed with high dietary carbohydrate level has significantly higher expression of gck, pk, hk, and ldh than the NC group (P < 0.05). In contrast, fish in the HC group had lower expression of irs1, igf-1, sdh, fbp, g6pc, gyp, and ampk compared to the NC fish (P < 0.05). On day 60 of the feeding trial, gck and hk expressions were still higher in the HC group (P < 0.05), and gyp, gdh, and ampk became increasingly expressed in the HC group. The increase of dietary carbohydrates resulted in significant increases in amylase and protease activity, plasma glucose, liver glycogen, crude protein, and lipid contents of the fish whole-body (P < 0.05). The high carbohydrate feeding reduced the fish growth rate but increased feed efficiency and did not affect mortality. In conclusion, giant gourami could utilize high carbohydrates due to a high amylase secretion, high modulation of carbohydrate metabolism, and large glucose storage capacity.

Effects of dietary carbohydrate levels on growth, metabolic enzyme activities and oxidative status of hybrid snakehead (Channa maculata ♀ × Channa argus ♂)

An 8-week feeding trial was aimed to explore the effects of dietary carbohydrate on growth performance, metabolic enzyme activities and oxidative status of hybrid snakehead (Channa maculata ♀ × Channa argus ♂). A total of 540 healthy hybrid snakehead with initial body weight of (39.4 ± 0.01) g were selected and randomly divided into 18 cages, with 30 in each cage. Six isonitrogenous and isolipidic diets with different levels of digestible carbohydrate (α-starch) levels (DCL) (0%, 5%, 10%, 15%, 20% and 25%) were fed to hybrid snakehead. Results showed that dietary carbohydrate supplementation had significant effects on final body weight (FW), weight gain (WG), feed conversion ratio (FCR) and protein efficiency ratio (PER) of hybrid snakehead in a third order polynomial (cubic) model, with the optimal level established at 10.2%–12.0%. Effects on Condition factor (CF) of hybrid snakehead was described by a second order polynomial (quadratic) relationship. Hepatosomatic index (HSI) and viscerosomatic index (VSI) showed a linear relationship with increasing DCL. The body protein, crude lipid and hepatic glycogen content of hybrid snakehead increased with the levels of carbohydrate supplementation (P < 0.05). Dietary supplementation with carbohydrate significantly increased the levels of plasma cholesterol (CHOL), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) in hybrid snakehead (P < 0.05). The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in both plasma and liver were significantly enhanced by the highest level of carbohydrate (P < 0.05). Furthermore, the levels of superoxide dismutase (SOD) and catalase (CAT) in liver increased firstly and then decreased with the increasing of DCL (P < 0.05). The expression levels of genes encoding SOD, glutathione peroxidase (GSH-PX), proinflammatory cytokines interleukin 1β (IL-1β) and interleukin 8 (IL-8) in liver showed significantly increasing trend when DCL increased from 0% to 15%, followed by significant decline when DCL exceeds 15% (P < 0.05). The malondialdehyde (MDA) contents in liver were not affected by DCL. Based on these results, the optimal level of dietary carbohydrate supplementation was estimated at approximately 11% for maximum growth and normal metabolism of hybrid snakehead.

Protein and carbohydrate content of infant formula purchased in the United States.

The protein and carbohydrate composition of formula fed infants' diets in the United States (US) has not been described. The aims of this study were to characterize these dietary exposures in infant formula purchased in the US and to estimate the proportion of formula purchased which is hypoallergenic or lactose-reduced formula. Powdered infant formula purchase data from all major physical stores in the US prior to the COVID-19 pandemic, between 2017 and 2019, were obtained from Information Resources, Inc. Protein and carbohydrate composition and scoop sizes for each formula were obtained from manufacturers. Ready to feed liquid products, products for premature infants and products for over 1year old were not included. Total volumes of term formula purchased were 216million kg of formula powder (equivalent to 1.65 billion litres) over 3 years. Intact protein formula was 67.9% of formula purchased, 26.6% was partially hydrolysed and 5.5% was hypoallergenic (5.2% extensively hydrolysed protein; 0.3% amino acid based). Soy protein formula represented 5.1% of formula purchased. Carbohydrate content overall was 52.7% lactose, 42.3% glucose polymers and 5.0% sucrose. 23.7% of formula purchased included sucrose as a carbohydrate. Of all formula purchased, 59.0% was lactose reduced, containing a non-lactose carbohydrate. Of 'standard' formula, defined as intact protein, non-thickened, cow's milk formula, 32.3% was lactose reduced. The proportion of hypoallergenic formula purchased significantly exceeded the prevalence of cow's milk protein allergy and increased over the 3-year study period from 4.9% to 7.6% of all formula sold. US infants are exposed to unnecessarily high levels of non-lactose carbohydrates and hypoallergenic formula, and this may represent a significant nutritional health risk.

Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment.

Melatonin (MT) is an important biologically active hormone that plays a vital role in plant growth and development. In particular, it has been investigated for its roles in abiotic stress management. In this study, pepper seedlings were subjected to low-temperature combined with low-light stress (LL) (15/5°C, 100 μmol m-2s-1) prior to a foliar spray of 200mM MT for 168h to investigate the protective role of MT in pepper seedlings. Our results demonstrated that LL stress negatively affected root growth, and accelerated the accumulation of reactive oxygen species (ROS), including H2O2 and , changed the osmolytes contents, and antioxidative system. However, these were reversed by exogenous MT application. MT effectively promoted the root growth as indicated by significant increase in root length, surface area, root volume, tips, forks, and crossings. In addition, MT reduced the burst of ROS and MDA contents induced by LL, enhanced the activities of SOD, CAT, POD, APX, DHAR, and MDHAR resulted by upregulated expressions of CaSOD, CaPOD, CaCAT, CaAPX, CaDHAR, and CaMDHAR, and elevated the contents of AsA and GSH, declined DHA and GSSH contents, which prevented membrane lipid peroxidation and protected plants from oxidative damages under LL stress. Furthermore, seedlings treated with MT released high contents of soluble sugar and soluble protein in leave, which might enhance LL tolerance by maintaining substance biosynthesis and maintaining cellular homeostasis resulted by high levels of osmolytes and carbohydrate in the cytosol. Our current findings confirmed the mitigating potential of MT application for LL stress by promoting pepper root growth, improving antioxidative defense system, ascorbate-glutathione cycle, and osmotic adjustment.

Adiponectin and adiponectin receptors in common carp (Cyprinus carpio): Tissue distribution and their expressions in response to high-carbohydrate and high-lipid diets

In order to investigate the roles of adiponectin in glucose and lipid metabolism in common carp, the tissue distribution of adiponectin and its receptor genes in common carp were firstly detected in this study, and then the effects of high-carbohydrate (45%) and high-lipid (11%) diets on their expressions were studied in the feeding trial. The results showed that adipoqa and adipoqb mRNA levels were highest in the red muscle, followed by the heart and white muscle. Adiponectin receptor genes were widely expressed in all tested tissues. Two subtypes of adiponectin receptor 1 genes (adipor1a and adipor1b) were expressed at the highest level in the brain, while adipor2 mRNA was highly expressed in the heart and red muscle. The high-carbohydrate diet significantly up-regulated adipoqa and adipoqb mRNA level in the heart of common carp, while the high-lipid diet significantly promoted adipoqa mRNA expression in the red muscle and heart, compared to the control diet. For adiponectin receptor gene expression, a high-carbohydrate diet up-regulated adipor2 mRNA level in the hepatopancreas, while a high-lipid diet significantly promoted adipor1a, adipor1b and adipor2 mRNA expression in the red muscle and hepatopancreas. The results showed that both high-carbohydrate and high-lipid diets could induce adiponectin genes expression in common carp, and AdipoRs expression was more likely to be increased by the high-lipid diet than the high-carbohydrate diet. This study suggested that adiponectin and its receptors were involved in the metabolic regulation of fish under high dietary carbohydrate and lipid levels.

Tiempo necesario para que las bacterias ruminales se adapten a los cambios en la dieta del ganado confinado: Revisión

The system of production in animals’ feedlot is an excellent strategy for periods of food scarcity and lack of space. In feedlot, concentrated diets with high levels of fast-digesting carbohydrates are used. This review was carried out with the objective of addressing the effect of feedlot diets on the ruminal bacterial population in cattle finished in feedlot. Ruminal bacteria respond rapidly to dietary changes, since they reproduce at intervals of less than 60 minutes. The time of colonization of ruminal bacteria indicates the speed that adhere and penetrate into the food. Rumen microorganisms can be associated with food particles at different times. The particle size of food is one of the important factors for degradation, since it determines the attack surface for microorganisms and directly influences the time of colonization of the food. There is a linear relationship between the body size of the animals and the rumination rate, evidencing that it relates the size of the rumen or digestive tract with the body weight of the animals. Restriction and ladder adaptation protocols are the most used by nutritionists in beef cattle in feedlot The inclusion of additives and probiotics is a valid strategy to improve utilization, as long as it is used at appropriate levels. To minimize the negative effects of changes in diets, it is necessary to gradually adapt the ruminal microbiota, either by the adaptation protocols by ladder or by restriction. Both protocols typically improve health and maximize the performance of animals with 14 days of adaptation.