High Biological Value Research Articles

Обоснование рациональных режимов термического выделения липидов из жиросодержащих рыбных отходов

Many fish waste remaining during the cutting of fish (heads, entrails, bones) contain an increased amount of fat, which has a high biological value. Obtaining edible fat from them is not always possible due to safety requirements, and the production of feed fishmeal with standard quality indicators is difficult. The increased fat content of raw materials and the technology parameters contribute to the rapid rancidity of fat in flour and the formation of toxic substances. This raw material can be used to obtain fish oil with subsequent recommendations for use, taking into account the indicators of oxidative and hydrolytic spoilage and in accordance with the requirements of the standards. When the content of undesirable products is exceeded, it can be used for technical purposes or in industrial biotechnology, as a substrate for microbial synthesis of biodegradable biopolymers such as polyhydroxyalkanoates. In this paper, a thermal method for separating fat from the heads of mackerel and hot-smoked sprats, as waste products from fish canning enterprises in the Kaliningrad region, was studied. A high fat content in raw materials (respectively 24.9 and 20.3%) and its characteristics were established. The rational parameters of the hydromodulus (1:0.5 and 1:1.0) were determined during thermolysis of crushed sprat and mackerel raw materials, respectively, at a stirrer rotation speed in the thermoreactor of 15 rpm. Rational ranges of temperature and duration of thermal release of fat were determined in experiments using mathematical planning. The optimization parameters were the yield of fat, its acid and peroxide numbers. The maximum yield of fat from fish heads is achieved at a temperature of 73-94°C for 2 hours 15 minutes - 2 hours 45 minutes. However, at the same time, fat has increased values of acid and peroxide numbers. Obtaining high-quality fat with food indicators is recommended at a temperature of 68 - 83 ° C for 43 min. – 1 h 22 minutes.

Biological value of the meat protein of Edilbaev lambs when enriching diets with es-sential microelements

The article presents the results of studying the quality and usefulness of the protein in meat products obtained from lambs of the Edilbaev breed. The experiment involved one control and three experimental groups of animals, the diet of which was enriched with essential microelements in the form of feed additives "Yoddar-Zn" and "DAFS-25". The results of the research showed that in the rams of the experimental group, which were fed in combination with the main diet supplements Ioddar-Zn and DAFS-25, the maximum amino acid score and protein-quality index were noted, which indicates a high biological value of meat. According to the coefficients of utility and comparable redundancy, it is assumed that in the meat of rams from the control group, amino acids are better balanced and can be more rationally used by the human body. Among the experimental groups of young sheep, the meat of the experimental group, where two feed additives were used at once, had the best rationality.

Biologically Active Compounds from Probiotic Microorganisms and Plant Extracts Used as Biopreservatives.

Ensuring the microbiological safety of food products is a pressing global concern. With the increasing resistance of microorganisms to chemical agents and the declining effectiveness of synthetic preservatives, there is a growing need for alternative sources of natural, bioactive compounds with antimicrobial activity. The incorporation of probiotics and plant extracts into food formulations not only enriches foodstuffs with microorganisms and phytochemicals with biologically active compounds, but also provides a means for product preservation. The current review considers the importance of the process of biological preservation for providing safe foods with high biological value, natural origin and composition, and prolonged shelf life, thereby improving consumers' quality of life. To accomplish this goal, this review presents a series of examples showcasing natural preservatives, including beneficial bacteria, yeasts, and their metabolites, as well as phenolic compounds, terpenoids, and alkaloids from plant extracts. By summarizing numerous studies, identifying research challenges and regulatory barriers for their wider use, and outlining future directions for investigation, this article makes an original contribution to the field of biopreservation.

Mint Plants (Mentha) as a Promising Source of Biologically Active Substances to Combat Hidden Hunger

Hidden hunger, also known as micronutrient deficiency, is a form of undernutrition, which is exacerbated when food security is fragile. However, the amount of phytonutrients in the diet can be increased by using underutilized species, such as fresh mint greens (Mentha). These plants have a high biological value due to the high content of biologically active substances. Plants of the genus Mentha, however, differ significantly in their chemical composition and, thus, nutritional value. The main objective of this study was to evaluate the content of phytonutrients in different Ukrainian species of mint: peppermint (Mentha piperita), horsemint (Mentha longifolia L.), silver-leaved horsemint (Mentha longifolia S.), and spearmint (Mentha spicata L.), and to determine their stability after drying and freezing. After studying the chemical composition of fresh, dried, and frozen plants of these species, it was established that Ukrainian mint species offer a robust set of phytonutrients and can be used as ingredients of the so-called “functional foods.” The biologically active substances in mint are concentrated during drying. Moreover, such raw materials are easily stored and used as an ingredient. However, the losses of ascorbic acid due to drying mint reach 70%; of carotenoids—approximately 10–15%; of chlorophylls—21–38%; and of phenols—19–29%. Peppermint and spearmint were observed to have higher stability of biologically active compounds. As for the freezing, the best stability of the chemical composition was demonstrated by field mint and spearmint samples. Therefore, a targeted selection of mint types and varieties prior to processing will allow preserving maximal preservation of a maximum amount of biologically active substances, increasing the content of phytonutrients in finished products, and preventing the development of hidden hunger.

Transforming livestock by-products into nutritious extruded feed additives: a sustainable approach for modern agriculture

The search for a solution to the challenge of providing the agriculture industry with complete feeds is relevant to modern animal husbandry in many countries, and protein is a key limiting component in feed. Along with the growth of food production, the waste it produces, which is also a valuable resource of useful components, can be recycled and used. Thus, in slaughter, butchering and meat production, a large amount of waste is generated, including by-products that can be processed further. The extrusion process is one of the best processing methods to improve the nutritional value of ingredients and feed and improve feed efficiency. In modern feed milling operations, extrusion must be considered as the main process for increasing feed profitability. The benefits of the extrusion process in improving the nutritional value and efficiency of ingredients and feeds depend on many factors, such as the structure and chemical composition of the ingredients, the processing conditions and the equipment used in processing. This article substantiates the need to develop technologies for involving production waste in their further use in medium- and long-term growth in the demand for food. A technology for producing an extruded feed additive based on vegetable feed with the addition of slaughter waste is presented. The formulation of a feed additive in which different percentages of slaughter waste of 5.10% and 15% corn was replaced with by-products was studied. The water activity of the obtained extruded feed additives was studied, where, at a content of 15% slaughter waste, it was low compared with that of the other two samples. Further research will allow procuring fodder products with a high biological value and utilising unclaimed by-products of livestock slaughter.

Green Assessment of Phenolic Acid Composition and Antioxidant Capacity of Advanced Potato Mutant Lines through UPLC-qTOF-MS/MS Quantification.

Potatoes are one of the most consumed crops worldwide. They contain a high amount of bioactive compounds such as phenolic compounds and vitamins with important antioxidant activities, which makes this crop of high biological value for human health. The goal of this research was to biochemically evaluate polyphenol levels and antioxidant capacities in parent and control genotypes compared to advanced mutant potato lines in the M1V8 generation. This will reveal the genetic changes that result from induced mutagenesis. The quantified compounds and the evaluated antioxidant activity boost the health benefits of consuming the improved mutant potatoes. In the present study, the phenolic composition and antioxidant activity of eighteen mutant and initial potato genotypes were analyzed by UPLC-qTOF-MS/MS and the ORAC method, respectively. In each of the hybrid combinations, mutant lines with an improved phenolic compound profile were observed. Representative samples from the third hybrid combination had notable increases in phenolic compound concentrations, as well as the presence of metabolites not found in the parental lines. With one exception, the remaining nine mutants showed significantly higher antioxidant capacities. The results will be used in future potato breeding programs, with participation of the valuable mutant lines containing new phenolic substances not present in the initial genotypes.

Cinnamon as a Potential Feed Additive: Beneficial Effects on Poultry Health and Production Performances – An Update

According to the Food and Agricultural Organization, global poultry output increased from approximately 115 million tons in 2016 to around 136 million tons in 2023. Poultry production has increased significantly with the dramatic uptick in meat and egg demand. Feed accounts for between 65 and 70 percent of total production costs, making it the largest chicken industry expense. This is why it's important to maximize the transformation of poultry feed into feed with a high biological value while taking as many steps as possible to protect feed quality and reduce feed costs. The use of feed additives in poultry feed has recently gained popularity and has been essential to increase feed efficiency and growth rate, which typically leads to reduced costs. The meat's texture, consistency, and nutritional content are all improved, and its shelf life is lengthened as a bonus. Feed additives are a fantastic tool for boosting a poultry farm's bottom line. For example, cinnamon (Cinnamomum verum) is often used as a traditional feed supplement. Rather than antibiotics, the poultry industry could benefit from using cinnamon as a natural antibiotic replacement, which would benefit animal welfare, consumer health, and the bottom line. The performance index, feed intake, FCE performance, and weight growth of poultry can all be improved by including cinnamon in the feed at varied concentrations. The digestive health and intestinal microbial population of hens are enhanced by a diet containing bioactive components of cinnamon. Cinnamon essential oils' popularity stems from their many valuable features, such as their ability to increase gastric enzyme synthesis and other biofunctional benefits. This review focuses on the possible advantages of cinnamon as a natural feed supplement for chickens, particularly about their intestinal microbiota, blood chemistry, nutrient absorption, gene expression, and immunology.

Optimization of technological parameters in the production of cereal beverages fortified with Omega-3 polyunsaturated fatty acids

The object of this study was cereal drinks fortified with Omega-3 polyunsaturated fatty acids: rice (product 1), oat (product 2), buckwheat (product 3). The purpose of the research was to optimize technological parameters in the production of such beverages. A mathematical model was built in the form of three regression equations describing the influence of grinding size (K), roasting temperature (Т), and roasting duration (t) on three output variables: oat, buckwheat, and rice protein content. Statistical analysis of the obtained equations showed that they are adequate in the selected area of planning: K=0.66–2.34 mm, T=133–217 °С, t=3.3–11.7 min. Studies were conducted to determine quality indicators, chemical composition, biological and nutritional value of the developed cereal drinks, in comparison with the control version. It has been established that the content of fatty acid composition of cereal drinks differs markedly. The minimum share of saturated fatty acids was established in product No. 3, without fortification – 10.43 %. The content of polyunsaturated fatty acids ranged from 82.09 % (product 1) to 89.57 % (product 3). The highest omega-3 content was found in product 2, with fortification – 1.34 %, and the lowest – in product 1, without fortification – 0.27 %. The introduction of a filler in cereal drinks made it possible to add protein, thereby increasing the biological value. According to the content of essential amino acids, the devised product is characterized by a high content of essential amino acids, exceeding the FAO/WHO ideal protein scale, which allows us to draw a conclusion about the high biological value of the developed product. The analysis of the mathematical model built established that an increase in the coarseness of grinding leads to a decrease in the protein content in the entire investigated range of values of this factor

The probiotic chestnut‐based dairy matrix: Influence on the metabolomic formation and the nutritional quality

The aim of this study was to characterise the impact of mixed probiotic strains and fortification with chestnut beverage (CB) on metabolomic formation in a plant‐based dairy matrix. Bacterial growth, nutritional composition and metabolomics were investigated during the period of 21‐day storage. The amino acid score (AAS), essential amino acid index (EAAI), nutritional index (NI) and biological value (BV) of the samples were determined to characterise their nutritional quality. The fortification with CB increased the nutritional quality of samples due to high EAAI, AAS, NI and BV. Using HPLC‐DAD, GC–MS and HS‐GC/MS‐based instrumental techniques, 48 metabolomics were identified.

Pumpkin fruit selection of different types and varieties for the production of functional food products

Consumers in Ukraine and around the world are increasingly interested in healthy lifestyles and functional foods with high biological value. For the production of such foods, it is important to select raw materials that meet a range of quality requirements. Pumpkin fruits have a high content of nutrients, vitamins, essential amino acids, and minerals that largely meet these requirements. The research aims to comprehensively evaluate pumpkin fruits of eight varieties of different types: large-fruited (Cucurbita maxima Duch) and butternut (Cucurbita moschata Duchex Poir), grown in the forest-steppe of Ukraine, to identify the most suitable for drying and production of functional foods. The experimental method was used following the research plan, the laboratory method was used to determine biochemical, biometric, and organoleptic quality indicators, and the statistical method was used to conduct dispersion and correlation analyses of the studied indicators. It has been established that when large-fruited pumpkin varieties are used for convective drying, 16.1-20.3% of dry products with a sugar content of 48.6-51.6% and 11-14 and 34.5-40.2% of nutmeg varieties, respectively, can be obtained. For the production of functional food products with a β-carotene content of 40-41 mg/100 g (in terms of dry matter), it is advisable to use the fruits of nutmeg varieties Gilea and Divo, and vitamin C at the level of 28 mg% – large-fruited varieties Slavuta and Polyovychka. The study revealed that with the increase in fruit weight, the content of dry matter (r=-0.68), sugars (r=-0.67) and the yield of finished products (r=-0.74) significantly decreases. A significant direct relationship between the content of dry matter and sugars (r=0.98), as well as the content of dry matter and the yield of finished products (r=0.94), was established. The materials of the article are of practical value for breeders, vegetable growers, and specialists of processing enterprises when choosing a type and variety of pumpkin for the production of functional foods

The History of Apricot Origin on the Territory of Kyrgyzstan

The author examines the history of the origin of apricots and their biology, ecology and distribution around the world. The history of distribution and development of apricot in the Kyrgyz Republic is analyzed. Varieties and their biology are described. The main ecological features of apricot varieties are presented. In the south of Kyrgyzstan, there are all natural opportunities for processing apricots as a cultural culture that ensures high quality products. The fruits of apricot varieties are a functional crop that can be used both dry and fresh, differing in technological quality, high biological vitamin value and mineral composition. For this reason, high-quality dried and kitchen apricot varieties have been grown in the south of Kyrgyzstan for many years.

Investigating the above-ground biomass values of sweet potatoes (Ipomoea batatas)

The role of sweet potato tubers in human nutrition is not new. The above-ground biomass of sweet potatoes is not used for nutritional purposes in most countries, but it has a high biological value. Therefore, the aim of the present study is to investigate the production of press fibre from above ground biomass by wet fractionation. Two sweet potato varieties (purple- and white-fleshed sweet potato) and two types of irrigation system were used: bubbling water flow system (BWS) and continuous water flow system (CWS). Glucan, xylan, arabinan were analysed by HPLC and elemental content was measured by ICP-OES. Our results show that the total carbohydrate content in the pressed fibre of the leaf blades (27.64–29.88 %w/w) is lower than in the stem with petiole (51.14–57.36 %w/w). No significant difference in glucan, xylan and arabinan content was observed in the leaf blade. In the stem with petiole, significant differences were observed for xylan and arabinan contents. For elemental content, generally higher values were measured in the leaf blade than in the stem with petiole. This information may be relevant for the selection of the appropriate variety and treatment, even for the production of functional food.

A Cross-Sectional Study: Animal and Vegetable Protein Intake Among Preschool Children

Protein is one of the macronutrients needed in child growth, which can be obtained from animal and plant foodstuffs. Protein intake gets more attention because energy-protein malnutrition is more common in children under five. Protein nutrition for children must be fulfilled to prevent growth retardation in the next phase. This research aims to establish a profile of preschool children's animal and vegetable protein intake. This type of research is descriptive observational with a cross-sectional design. This research was conducted at Bina Anasprasa Nuris Jember Kindergarden in March 2023. The sample in this study was preschool children aged 3-5 years, totaling 53 people who were taken by purposive sampling. Food consumption data for children was obtained using the 1x24-hour food recall method. Processing and analysis of data using univariate analysis. Preschool girls have more than boys (67.9% and 32.1%). The majority of mothers are not working/housewives (62.3%), while the majority of fathers are self-employed (45.3%). Most families have income above the 2023 Jember UMK (62.3%). Nutritional status with indicators of weight/age, height/age, weight/height of all preschool children has normal, normal, and good nutritional status (100%). Total protein intake increased slightly for boys and families whose income was above the minimum wage. Meanwhile, the intake of vegetable and animal protein sources in preschool children is similar but slightly higher in vegetable protein. Animal protein food ingredients still have high biological value because they contain all essential amino acids compared to vegetable protein. Therefore, preschoolers need to increase their consumption of animal protein food ingredients in their daily diet.

Actual problems of the quality and safety of milk and dairy products

Milk and dairy products have a high nutritional and biological value, which causes a great demand for them. The quality and safety of raw milk and dairy products on the way to their production due to potential risks are of great concern. Milk is a good environment for the reproduction of many microorganisms, including dangerous ones: Salmonella Escherichia coli O157^H7, Listeriamono cytogenes, Staphilococcus aureus, Bacillus cereus, Clostridium botulinum, Micobacterium bovi, Brucellaa bortus and Brucellamelitensis Yersiniaentero colitica. The safety and quality of raw milk in Ukraine remains the biggest problem of dairy enterprises reequipped with the latest equipment, technological lines and modern quality control systems. An urgent problem is the quality and safety of milk from cows that are kept in auxiliary farms of the population. According to the results of bacteriological studies in raw milk and dairy products (fermented milk cheese, sour cream) from the auxiliary farms of the population, which are sold in food markets, St. aureusvar. bovis and St. aureusvar. hominis. A low level of sanitary culture causes an increased rate of MAFAnM and psychrophilic microflora in raw milk. In order to increase competitiveness, it is additionally necessary to introduce regulations on the content of psychrophilic microflora. Mastitis is a significant problem in reducing the productivity and quality and safety of milk. Somatic cells are the criterion for determining the quality and safety of raw milk and the state of health of the mammary gland of animals. In pursuit of profit, in order to increase sales of their goods and reduce their cost, many Ukrainian enterprises that produce butter resort to falsifying it with margarine, which includes 80% vegetable oil, emulsifiers, flavorings, vitamins and preservatives. In addition to a significant number of biological pollutants, chemical, physical and inadequate (foreign bodies, allergens, mycotoxins, industrial pollution) pose a danger. Therefore, the prevention of the impact of dangerous substances on the health of the population should be based on the management of the quality and safety of milk and dairy products throughout the food chain "from the farm to the table". Key words: raw milk, dairy products, falsification, quality and safety indicators, biological, physical and chemical risks.

Modification and Solubility Enhancement of Rice Protein and Its Application in Food Processing: A Review.

Rice protein is a high-quality plant-based protein source that is gluten-free, with high biological value and low allergenicity. However, the low solubility of rice protein not only affects its functional properties such as emulsification, gelling, and water-holding capacity but also greatly limits its applications in the food industry. Therefore, it is crucial to modify and improve the solubility of rice protein. In summary, this article discusses the underlying causes of the low solubility of rice protein, including the presence of high contents of hydrophobic amino acid residues, disulfide bonds, and intermolecular hydrogen bonds. Additionally, it covers the shortcomings of traditional modification methods and the latest compound improvement methods, compares various modification methods, and puts forward the best sustainable, economical, and environmentally friendly method. Finally, this article lists the uses of modified rice protein in dairy, meat, and baked goods, providing a reference for the extensive application of rice protein in the food industry.

Breeding Population and Nesting Habitat of Skuas in the Harmony Point Antarctic Specially Protected Area

Regular monitoring of breeding population abundance and environmental factors related to the nesting habitat has proven fundamental for seabird conservation. Harmony Point (Nelson Island) is an Antarctic Specially Protected Area (ASPA) designated to manage and protect the high biological value of seabirds’ richness and abundance. However, due to the remote location of their breeding sites, many species lack updated population counts. Skuas (Catharacta sp.) exhibited a two-decade gap since the last census was conducted in Harmony Point. The abundance and spatial distribution of the nests of skuas were studied during the austral summer of 2019/2020. Through an exhaustive search, we counted and mapped active nests. Nesting habitat was assessed by the use of an unmanned aerial vehicle to take aerial pictures and build an orthomosaic image to determine vegetation in the area. Additionally, a digital elevation model was built to calculate a series of geomorphological-related variables. Suitability models were used to estimate the importance of variables to the nesting of skuas. A total of 71 brown skua (Catharacta antarctica) and 3 south polar skua (C. maccormicki) nests were counted. Two of the seven variables (terrain slope and vegetation cover) accounted for 57.5% ± 14.1% of the models’ variability; sun radiation incidence, and wind shielding were of secondary importance. Water flow accumulation, distance from penguin colonies, and terrain elevation were the least important variables. Skuas selected for nesting flat terrains (slope < 10°) with a vegetation cover of above 20%, slightly higher sun incidence (270 to 280 kW/h), and intermediary windshielding (45% to 55% of exposition). Considering previous estimates, the skua species at Harmony Point has kept an apparently stable population size over the last 25 years. However, expected changes in nesting habitat availability, i.e., increased snow-free area, increased wind intensity, changes in vegetation cover, and reduction of penguin populations, might change population size in the mid to long term.

Comparation of moist heating and formaldehyde protein protection methods and their impact on dairy ration fermentability and digestibility

Improving protein efficiency has become the main target in dairy farming. The feeding strategy for high-producing cows is directed by optimizing non-protein nitrogen utilization while maintaining post-ruminal digested bypass protein. Soybean and soybean meal are protein sources with high biological value but are highly degradable, so they must be protected. This study aimed to compare the effect of moist heating and formaldehyde treatments as a method for protecting protein to increase rumen bypass protein. Parameters observed include fermentation profile (rumen pH, NH3, total VFA, protozoa, and total bacteria population) and nutrient digestibility. Treatments tested were an unprotected diet as control (C1), moist heating protected protein (C2), and formaldehyde protected protein (C3). Data were subjected to ANOVA using Randomized Block Design followed by orthogonal contrasts. The results showed that both moist heating and formaldehyde treatments (C2 and C3) reduced NH3 and total VFA concentration (p<0.05) without affecting rumen pH, protozoa, and total bacteria count (p>0.05). Dry matter and organic matter did not affect by the treatments. In conclusion, both moist heating and formaldehyde methods could be used to protect feed protein and improve bypass protein in the dairy ration.

Reptile Bushmeat, an Alternative for the Supply of High Biological Value Proteins?

The sustainable use of wildlife is considered a tool for conservation in addition to generating benefits for the local population. Commercial reptile hunting targets skins, generating other by-products such as meat and fat. Meat from hunted reptiles is widely available in localities where management plans are in place and is evaluated as easily accessible for hunters and their families. The objective of our research was to evaluate the percent composition, protein composition and microbiological quality of black and white tegu and yellow anaconda meat. For this study, we obtained meat samples of both species from wild specimens. The composition of both meats showed a proportion of moisture greater than 70%, a good proportion of protein (around 20%) and a low proportion of intramuscular fat (&lt;2%). In the meat of black and white tegu, we found all of the essential amino acids in the recommended proportions, while the meat of yellow anaconda tryptophan did not reach the recommended levels to meet adult requirements. Both meats had good microbiological quality and were free from pathogenic bacteria. The results obtained reveal distinctive nutritional qualities of the meats analyzed, which can be recommended as an alternative and/or complementary source of good quality protein for human consumption.

A Review of Ketogenic Dietary Therapies for Epilepsy and Neurological Diseases: A Proposal to Implement an Adapted Model to Include Healthy Mediterranean Products.

Based on the growing evidence of the therapeutic role of high-fat ketogenic dietary therapies (KDTs) for neurological diseases and on the protective effect of the Mediterranean diet (MD), it could be important to delineate a Mediterranean version of KDTs in order to maintain a high ketogenic ratio, and thus avoid side effects, especially in patients requiring long-term treatment. This narrative review aims to explore the existing literature on this topic and to elaborate recommendations for a Mediterranean version of the KDTs. It presents practical suggestions based on MD principles, which consist of key elements for the selection of foods (both from quantitative and qualitative prospective), and indications of the relative proportions and consumption frequency of the main food groups that constitute the Mediterranean version of the KDTs. We suggest the adoption of a Mediterranean version of ketogenic diets in order to benefit from the multiple protective effects of the MD. This translates to: (i) a preferential use of olive oil and vegetable fat sources in general; (ii) the limitation of foods rich in saturated fatty acids; (iii) the encouragement of high biological value protein sources; (iv) inserting fruit and vegetables at every meal possible, varying their choices according to seasonality.

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate.

Special attention is being paid to the study of amaranth proteins. They are characterized by a high biological value that significantly exceeds those of grain crops. The production of protein concentrate from amaranth flour includes preliminary enzymatic hydrolysis, extraction of the resulting mixture, protein precipitation, microfiltration, and freeze-drying. In our study, the obtained amaranth protein concentrate was limited by valine, with an amino acid score of 74%. The true digestibility of the amaranth protein concentrate determined in vivo was 97.6 ± 0.3%, which was significantly lower than that of casein (99.3 ± 0.2%). The protein digestibility-corrected amino acid score value of the concentrate was 72.2%. The obtained concentrate was a rich source of selenium, copper, magnesium, manganese, and iron. Ferulic acid was the only polyphenolic compound found in the amaranth protein concentrate, but its content was significantly greater compared to the original flour. Saponins were not removed completely during the process of obtaining the amaranth protein concentrate. We identified 15 saponins in the concentrate, mainly of the bidesmoside type, the sapogenins of which are related derivatives of oleanolic acid. Thus, the developed amaranth protein concentrate can be used as an ingredient in functional food products, with a high biological value.