Hibiscus Rosa-sinensis Flowers Research Articles

An assessment and characterization for biosorption efficiency of natural dye waste

The local natural dyeing house run by a women’s self-help group was generating a huge amount of dye extracted pulp. We thought of using this pulp for an alternative use other than just composting it. This biomaterial was found to be excellent heavy metal adsorbents and was demonstrated to be very effective in remediation of groundwater and surface water of chrome metals in contaminated sites of tannery operations. In this article, sorption efficacy studies were carried out on selected biomaterials known for their potential usage as natural dyes. Canna indica flower, Portulaca olecera flower and stem, Hibiscus rosa sinensis flower and Trapa natans fruit skin (exocarp) were used for sorption studies. These plant parts, after the extraction of natural dye, were dried and evaluated for biosorption of heavy metal from effluent. Batch tests indicated that hexavalent chromium sorption capacity (qe) followed the sequence qe(Trapa) >qe(Hibiscus) >qe(Portulaca) >qe(Canna). Due to high sorptive capacity, Trapa fruit skin (exocarp) was selected. The optimization studies were carried out by taking the Trapa exocarp in powdered form, and of particular mesh size. Sorption kinetic data have shown first order reversible kinetic model for all the sorbents, however the biosorption of chromium by T. natans biomass occurred in two stages. In the first stage, 95% sorption was reported for Chromium (VI) in 15 min followed by a slower second stage. It reached equilibrium in 1 h at which 90–98% of the Chromium (VI) was biosorbed by T. natans. Experimentally reported equilibrium data fitted well with both the Langmuir and Freundlich Isotherms. The FT-IR, XRD and XPS analysis showed that the main mechanism of Chromium (VI) biosorption onto Trapa dried powder was through the binding of Chromium ions with amide group of the biomass.

Acute and Chronic Effect of Hibiscus rosa sinensis Flower Extract on Anxiety Induced Exploratory and Locomotor Activity in Mice

Acute and Chronic Effect of Hibiscus rosa sinensis Flower Extract on Anxiety Induced Exploratory and Locomotor Activity in Mice

Cytotoxicity of Hibiscus rosa-sinensis flower extract

Abstract Many species of Hibiscus are grown for their showy flowers or used as landscape shrubs. Hibiscus has also medicinal properties. Flowers of these plants are rich in polyphenols, flavonoids and anthocyanins. In this study flower decoction of Hibiscus rosa-sinensis has been tested for cytotoxic activity. Allium cepa L. has been used for evaluating cytotoxicity. Decoction of flowers was toxic on root output and length in A. cepa L. and reduced the mitotic index significantly. It can be suggested that Hibiscus rosa-sinensis flowers contains antimitotic constituents which stop the cell division in anywhere of the cell cycle.

Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat.

BackgroundThe present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects.MethodsThe medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose) have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms) in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis.ResultsThere was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS) [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress) occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury.ConclusionIt may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury.

Blastocyst implantation failure in mice due to “nonreceptive endometrium”: endometrial alterations by Hibiscus rosa-sinensis leaf extract

Many plants are known to possess antifertility activity. However, limited attempts have been made to scientifically evaluate these claims. Hibiscus rosa-sinensis flowers have been shown to possess antifertility and abortifacient activity. In this report, antiimplantation activity of water extract of leaves of H. rosa-sinensis was investigated. Pregnant female mice were dosed with extract (100 mg/kg body weight) from days 1 to 6 of pregnancy. No implantation sites were observed in treated animals when they were surgically opened on day 15 of pregnancy. Biochemical and biophysical alterations were observed in the endometrium in treated animals, especially on day 5, at 4:40 a.m., the day of implantation. A sharp increase in superoxide anion radical and a sharp fall in superoxide dismutase (SOD) activity, as seen in the endometrium from control animals, were altered in treated animals. The extract also exhibited antiestrogenic activity, as judged by increase in uterine weight. The physiological alterations induced by water extract of H. rosa-sinensis are discussed.

Mating aggregations and mating success in the flower thrips, Frankliniella schultzei (Thysanoptera: Thripidae), and a possible role for pheromones

Aggregations of Frankliniella schultzei males were observed on the corollas of Hibiscus rosasinensis and Gossypium hirsutum flowers in southeast Queensland. Aggregations were seen only on the upper surfaces of corollas but may have occurred on other flower parts, which were hidden from view. Conspecific females entered aggregations and a small proportion of them mated [18% (n = 163), H. rosasinensis; 30% (n = 181), G. hirsutum]. Most females (87 and 72%, respectively) that did not mate in aggregations walked to other flower parts. Behavior was difficult to observe on these parts, but mating was sometimes observed there. The number of females that landed within aggregations on the upper surfaces of both H. rosasinensis and G. hirsutum corollas was highly correlated with the number of males (r = 0.88, r = 0.93, respectively; P < 0.001). Significantly more mating pairs were observed in high-density aggregations (mean +/- SE, 1.10 +/- 0.22 and 4.44 +/- 0.48, respectively) than in low-density aggregations (0.37 +/- 0.11 and 1.67 +/- 0.29, respectively) (P < 0.05) on flowers of both species. More F. schultzei females were attracted to sticky traps baited with live conspecific males set among flowering Ipomoea indica (mean +/- SE, 8.83 +/- 0.32) and G. hirsutum (10.90 +/- 0.79) plants than to control traps (0.10 +/- 0.05 and 0.70 +/- 0.25, respectively)( P < 0.05), presumably in response to male-produced pheromones. Significantly more females were attracted to traps with high male densities than to traps with low densities. We found no statistical evidence that aggregation size influenced mating success (proportion males that mated). Mating success, however, should be evaluated with respect to mating on all flower parts and not just the upper surfaces of corollas. The results of this study constitute the first behavioral evidence for an attractant sex pheromone in thrips.

Flowers of Hibiscus rosa-sinensis, a potential source of contragestative agent. III: Interceptive effect of benzene extract in mouse

In mouse, oral administration of the benzene extract of Hibiscus rosa-sinensis flowers at a dose level of 1 gm/kg body weight/day 5–8 of gestation led to termination of pregnancy in about 92% of the animals. The effect was associated with a significant fall in peripheral level of progesterone and increase in uterine acid phosphatase activity, as measured on day 10. The ovary exhibited signs of luteolysis, and the corpus luteal Δ 5-3 β-hydroxysteroid dehydrogenase activity decreased markedly. The interceptive effect of the extract was prevented completely by exogenous progesterone (1 mg/mouse/day) or chorionic gonadotropin (1 1.μ./mouse/day) and partially (62.5%) by exogenous prolactin (500 ug/mouse/day). In unilaterally pregnant mouse having traumainduced deciduomata in the sterile horn, the extract caused resorption of the fetuses, and regression of the deciduomata accompanied by reduction in weight of the ovaries. Luteolysis, may be due to interference with the luteotropic influence, and a consequent fall in plasma level of progesterone have been suggested as the plausible cause of termination of pregnancy.

Flowers of [formula omitted], a potential source of contragestative agent: II. Possible mode of action with reference to anti-implantation effect of the Benzene extract

Benzene extractives of Hibiscus rosa-sinensis flowers, administered during day 1–4 of gestation, exerted anti-implantation effect without affecting the tubal transport of zygote. On day 4, normal number of blastocyst was present in the uterus but they did not implant. However, as studied by pontamine blue reaction, it was evident that hyper-permeability of the endometrial capillaries which is the earliest known response of a receptive endometrium to any kind of diciduogenic stimulus was inhibited by the extract. The magnitude of decidualization, as assessed by weight of the traumatized uterine horn and supported by the histological pictures of the uteri was significantly lower in comparison to that of the controls. Ovarian structure exhibited signs of luteolysis. Inadequate progestational development of the endometrium due to interference with the conditioning of the uterus with progesterone during prenidatory phase of pregnancy has been suggested as the plausible cause of the extract-induced implantation failure.

Flowers of [formula omitted], a potential source of contragestative agent: I. Effect of benzene extract on implantation of mouse

In mouse, the benzene extract of Hibiscus rosa-sinensis flowers was administered at four different dose levels (250–1000 mg/kg body weight/day) from day 1–4 postcoitus. Anti-implantation response and associated changes in the uterine chemical composition were studied. With an increase in the dosage of the extract, the percentage of implantation failure increased. At the dose level of 1 gm/kg body weight the extract led to failure of implantation in 93% of the mice. The effect was accompanied by adversely altered uterine weight, its protein content and alkaline and acid phosphatase activity. In another experiment, influence of the extract on uterine uptake of progesterone was studied in bilaterally ovariectomized mice treated with or without estrogen. It exerted neither inhibitory nor stimulatory influence on uterine progesterone uptake in untreated castrated mice but the estrogen-induced increase in the uptake level was significantly inhibited by the extract. Failure of uterine bed preparation due to antiestrogenic potentiality of the extract has been discussed as the plausible cause of implantation failure.

Anti-fertility activity of a benzene extract of Hibiscus rosa-sinensis flowers on female albino rats.

Various extracts of HIBISCUS ROSA-SINENSIS flowers have been studied for their antifertility activity on female albino rats. Benzene extract was found to be the most effective. For isolation of active constituents, the benzene extract was separated chemically into a number of fractions and their antifertility activities were studied systematically. The ether soluble portion of the water insoluble fraction of a benzene extract showed significant anti-implantation and abortifacient activities.

Effects of Hibiscus rosa sinensis on pregnancy of rats.

The total benzene extract of Hibiscus rosa sinensis (China rose plant) flowers showed potent, orally effective antifertility activity in albino rats when administered from day 1 to 10 of pregnancy. This activity of the extract was found to depend upon the dose and duration of treatment. The extract was not found to affect the tubual transport of the zygote as well as blastocyst per se from implanting. However, as studied by pontamine blue reaction and administration of extract during implantation, it seems that, at least in 70 % of the females, the antifertility activity of the drug was mediated via interruption in implantation. The results obtained by delayed implantation technique further suggest that the extract, in one way or other, alters the delicate estrogen-progesterone balance. The pregnancy maintenance by higher dose of oestrone in extract treated females further lend support to the curtailment of estrogen. However, invoking „anti-implantation factor” alone, is not responsible enough to achieve full contraception and the drug causes obsorption of foetus.

The Development of the British Rabbit Industry

In the present study, we propose a novel field-scale analytical tool for rapid screening of microbiological quality in raw milk using aqueous extracts of plant materials, i.e. flowers (e.g. Hibiscus rosa-sinensis L. and Clitoria ternatea), taproots (e.g. Beta vulgaris) and pricklypears (e.g. Opuntia dillenii). For each plant extract, the colour changes in raw milk were evaluated between 4.5 and 6.5 pH against the resazurin dye as a control. The plant anthocyanin content in each extract was analyzed by the differential pH method using a spectrophotometer. The Hibiscus rosa-sinensis flower extract was opted to further test since it had a pH-sensitive colour change (6.5; maroon to 6.2; light-pink) compared to other plant extracts, which did not indicate a noticeable colour variation with pH. Anthocyanin content of the Hibiscus extract was 0.59 g/mL. The novel method showed high linearity (R2 = 0.95), 100% accuracy and greater repeatability with an intermediate level of precision. The limit of quantification and detection was 0.46 and 0.15 g/mL, respectively. In conclusion, we demonstrated the potential in using water extract of Hibiscus rosa-sinensis L. flowers as an alternative to the resazurin dye reduction method for rapid and accurate microbiological quality control for raw milk procurement in remote areas.