Abstract
BackgroundThe present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects.MethodsThe medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose) have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms) in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis.ResultsThere was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS) [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress) occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury.ConclusionIt may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury.
Highlights
The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, in terms of its antioxidant effects
The initial investigations of Rona et al [4] demonstrated that the subcutaneous administration of isoproterenol (ISO) in rats produced graded myocardial necrosis ranging from patchy subendocardial necrosis to transmural infarction, while maintaining a patent coronary vasculature
The principal finding of the present study is that ischemic reperfusion injury (IRI) was associated with oxidative stress, as evidenced by increase in myocardial thiobarbituric acid reactive substances (TBARS) and depletion of myocardial endogenous antioxidant status (SOD, GSH and catalase)
Summary
The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, in terms of its antioxidant effects. Reperfusion of ischemic myocardium is accompanied by the development of oxidative stress and the generation of free radicals which plays a major role in the etiopathogenesis of reperfusion injury [3]. The initial investigations of Rona et al [4] demonstrated that the subcutaneous administration of isoproterenol (ISO) in rats produced graded myocardial necrosis ranging from patchy subendocardial necrosis to transmural infarction, while maintaining a patent coronary vasculature. The exact mechanism of ISO induced myocardial injury has not been clarified, but a mismatch of oxygen demand versus supply in the presence of coronary vasculature [7] leads to the present investigation related with oxidative stress (induced by isoproterenol) in in-vivo model
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