This study aimed to determine the volumetric effects on the upper airways of growing patients with Class II malocclusion treated with the Herbst appliance (HA). Volumetric measurements of the upper airways of 42 skeletal Class II malocclusion patients (mean age: 13.8±1.2years; ranging from 12.0 to 16.9years) were assessed using cone-beam computed tomography scans acquired before treatment (T0) and approximately 1year later (T1). The sample comprised a Herbst appliance group (HA group [HAG]; n=24), and a comparison group (comparison group [CG]; n=18) of orthodontic patients who had received dental treatments other than mandibular advancement with dentofacial orthopedics. In CG, nasopharynx and oropharynx volumes decreased slightly during the observation period (9% and 3%, respectively), whereas the nasal cavity volume increased significantly (12%; P=0.046). In HAG, there was an increase in the volume of all regions (nasal cavity, 5.5%; nasopharynx, 11.7%; and oropharynx, 29.7%). However, only the oropharynx showed a statistically significant increase (P=0.003), presenting significant volumetric changes along the time (T1-T0) in HAG. Mandibular advancement with the HA significantly increased the volume of the oropharynx, but no significant volumetric modifications were observed in the nasal cavity and nasopharynx.