Extensive use of the herbicide glyphosate leads to a high detection rate in the environment and potential risks to nontarget aquatic life. China ranks first globally in the production and consumption of glyphosate, but there are no glyphosate water quality criteria (WQCs) for protecting aquatic life. Here, data on the acute and chronic toxicity of glyphosate and glyphosate-based formulations (GBFs) to freshwater aquatic life were collected and screened. Significant differences in species sensitivity distributions (SSDs) and toxicity values for acute or chronic toxicity were found between glyphosate and GBFs. The hazardous concentrations for 5% of species (HC5) of glyphosate or GBFs between native and nonnative species were different, and native species were found to be more sensitive to the toxicity of glyphosate. The acute and chronic WQCs derived with the SSD method for glyphosate based on the toxicity data for native species in China were 3.35 and 0.26mg/L, respectively, and those found for GBFs were 0.21 and 0.005mg/L, respectively. The WQCs in this study were quite different from those estimated using similar statistical extrapolation methods in other countries, which reflects the differences in species sensitivity to glyphosate toxicity in different regions. The hazard quotients (HQs) were calculated based on the WQCs and concentrations of glyphosate in some surface waters in China and indicated that glyphosate exhibits medium or high hazard risk in some samples of Tai Lake, surface water in Guiyang, fishpond water in Chongqing, rural drinking water, and surface water and reservoir water in Henan Province. The WQCs of glyphosate and GBFs have scientific significance for the exposure and pollution control of herbicide formulations and the protection of aquatic life in China.