Henna Extract Research Articles

UJI AKTIVITAS ANTIDIABETES EKSTRAK DAUN PACAR AIR (Impatiens balsamina L.) TERHADAP GINJAL TIKUS WISTAR (Rattus novergicus L.) YANG DIINDUKSI ALOKSAN

Background: Diabetes mellitus is a serious, "chronic" condition that occurs when there is an increase in a person's blood sugar levels because the body is unable to produce enough insulin hormones/cannot effectively use the insulin produced. A lack of insulin, or the inability of cells to respond to it, causes high blood glucose levels (hyperglycemia), which causes clinical indicators of diabetes. Methods: This research is an experimental study using a completely randomized design. Twenty-four male white rats were divided into 6 groups consisting of normal controls (KN) who received no treatment, negative controls (K-) who were injected with alloxan at a dose of 150 mg/kg BW, positive controls (K+) who were injected with alloxan at a dose of 150 mg/kg BW. kg BW and glibenclamide at a dose of 0.09 mg/200 g BW, Treatment 1 (P1) was injected with alloxan at a dose of 150 mg/kg BW and ethanol extract of water henna leaves at a dose of 450 mg/KG BW, Treatment 2 (P2) was injected with alloxan with a dose of 150 mg/kg BW and ethanol extract of water henna leaves with a dose of 500 mg/KG BW, Treatment 3 (P3) was injected with alloxan at a dose of 150 mg/kg BW and ethanol extract of water henna leaves with a dose of 550 mg/KG BW. Mice blood glucose levels were measured on days 0 (after alloxan induction), 3, 7, and 14. Before the measurements, the mice were fasted for 8 hours. Results: Research shows that administration of water henna leaf extract has a significant effect in reducing urea and creatinine levels, as well as improving kidney morphology. However, it could not prevent the alloxan-induced increase in kidney weight in white mice. Conclusion: Administration of henna leaf extract (Impatiens balsamina L.) at a dose of 500 mg/kg BW had a significant effect on reducing urea and creatinine levels in white rats induced by alloxan. Administration of water henna leaf extract (Impatiens balsamina L.) at a dose of 450 mg/kg BW had a significant effect in improving kidney morphology in alloxan-induced white rats, whereas the organ index of water henna leaf extract could not prevent the increase in kidney weight in white mice induced alloxan

Study of some active substances and biological activity of extracts of henna leaves collected from Umm al-Qandil area -Libya (Lawsonia inermis)

The study aims to detect the active ingredients in the plant and to know their biological effectiveness against bacteria. The pH and humidity of the plant were measured. Aqueous and alcoholic extracts were prepared to obtain the crude extract in order to estimate its effectiveness against bacteria. The study showed the effectiveness of henna leaves (Lawsonia inermis) and their inhibition of the growth of some types of tested bacteria, including Staphylococcus, Strep Pyogenes, Escherichia Coli, salmonella, and Shigella. The inhibition results of the extracts varied and depended on the extraction method, temperature, and solvent used. The results of the initial chemical examination also showed that these extracts contained some important active ingredients such as (tannins, saponins, flavonoids, glycosides, resins, alkaloids), which made this plant used as a treatment for infections. The pH was estimated, and thus the importance of this plant increases as it can be used as a natural antibiotic. Therefore, we recommend studying the quantity and quality of the active compounds and knowing their molecular structure with the aim of developing natural extracts and dispensing with manufactured compounds due to the harm they cause to the living organism. Keywords: henna plant, qualitative findings, aqueous and alcoholic plant extracts, some types of pathogenic bacteria

Synthesis of Green Iron Nanoparticles Using Henna Plant Extract for Seed Germination and Vegetative Growth of Trigonella foenum-graecumL

This paper presents the green synthesis of iron nanoparticles (FeNPs). Iron sulfate served as the substrate and henna extract as the reducing agent in the synthesis of FeNPs. FeNPs were analyzed using a variety of techniques, including UV-visible spectroscopy, nano zeta-sizer analysis, and Fourier transform infrared spectroscopy (FTIR). By using UV-visible spectroscopy, the electron transition band of iron oxide, or the peak at 291 nm, was found to be the source of the FeNPs. The characteristic bands of iron oxide were detected at 790 by FTIR. These bands are associated with the stretching vibration of Fe-O at 1022 and 1516 cm-1. to determine how applying different combinations of nano-fertilizers affects the growth and yield of fenugreek. The experiment consisted of nine treatments. The results demonstrated that T8 performed better than the other treatments in terms of all elements of vegetative development and yield contents. The T8 yields plant height of 43.12 cm, fresh weight of 30.41g, dry weight of 2.44g, seed weight of 5.09g, and pod weight of 7.39g with an increase rate of (55.56, 82.97, 162.36, 105.24, and 122.59)%.

Synthesis and evaluation of biological effects of modified graphene oxide nanoparticles containing Lawson (Henna extract) on gastric cancer cells.

Targeted Graphene Oxide (GO) nanoparticles can play an important role in the treatment of cancer by increasing cancer cell targeting. This study was conducted to synthesize GO nanoparticles functionalized with chitosan-folate (CS-FA) to deliver a natural product Lawsone (LA) for cancer treatment. After characterization of the LA-GO-CS-FA, antioxidant activities of the nanoparticles were investigated by ABTS, DPPH, and FRAP tests. CAM assay was used to study the effect of nanoparticles on angiogenesis. The expression level of inflammatory and angiogenic genes in cells treated with nanoparticles was evaluated by real-time PCR. The findings demonstrated the formation of nanoparticles with a size of 113.3nm, a PDI of 0.31, and a surface charge of + 11.07mV. The percentages of encapsulation efficiency were reported at 93%. Gastric cancer cells were reported as the most sensitive to treatment compared to the control, and the gastric cancer cells were used to study gene expression changes. The anti-angiogenic effects of nanoparticles were confirmed by reducing the average number and length of blood vessels and reducing the height and weight of embryos in the CAM assay. The reducing the expression of genes involved in angiogenesis in real-time PCR was demonstrated. Nanoparticles displayed high antioxidant properties by inhibiting DPPH and ABTS radicals and reducing iron ions in the FRAP method. The reduction of pro-inflammatory genes in AGS cells which were treated with nanoparticles indicates the anti-inflammatory properties of nanoparticles. This study showed the efficacy of nanoparticles in inhibiting gastric cancer cells by relying on inhibiting angiogenesis.

Anti-Yeasts, Antioxidant and Healing Properties of Henna Pre-Treated by Moist Heat and Molecular Docking of Its Major Constituents, Chlorogenic and Ellagic Acids, with Candida albicans and Geotrichum candidum Proteins.

Lawsonia inermis, known as henna, has traditionally been utilized in cosmetics and folk medicine because of their valuable health effects. A lack of information about the processes that increase or decrease release, as well as the biological activities of constituents of natural origin, is an important pharmacological problem. This investigation evaluates the influence of moist heat on the flavonoid and phenolic contents of henna powder and their biological activities. HPLC analysis reflected the existence of 20 and 19 compounds of flavonoids and phenolics in the extract of unpre-treated henna by moist heat (UPMH) and pre-treated henna by moist heat (PMH). Several compounds such as chlorogenic acid, ellagic acid, rutin, rosmarinic acid, kaempferol, and pyrocatechol occurred with high concentrations of 57,017.33, 25,821.09, 15,059.88, 6345.08, 1248.42, and 819.19 µg/mL UPMH while occurred with low concentrations of 44,286.51, 17,914.26, 3809.85, 5760.05, 49.01, and 0.0 µg/mL, respectively in PMH. C. albicans, C. tropicalis, and G. candidum were more affected by UPMH with inhibition zones of 30.17 ± 0.29, 27 ± 0.5, and 29 ± 1.5 mm than PMH with inhibition zones of 29 ± 0.5, 25.33 ± 0.58, and 24.17 ± 0.29 mm, respectively. UPMH henna exhibited less MIC and MFC against the tested yeasts than PMH. Moreover, UPMH henna showed good wound healing, where the rat of migration, wound closure %, and area difference % were 14.806 um, 74.938 um2, and 710.667% compared with PMH henna 11.360 um, 59.083 um2, 545.333%, respectively. Antioxidant activity of UPMH and PMH henna. Promising antioxidant activity was recorded for both UPMH or PMH henna with IC50 5.46 µg/mL and 7.46 µg/mL, respectively. The docking interaction of chlorogenic acid and ellagic acid with the crystal structures of G. candidum (4ZZT) and C. albicans (4YDE) was examined. The biological screening demonstrated that the compounds had favorable docking results with particular proteins. Chlorogenic acid had robust behavior in the G. candidum (4ZZT) active pocket and displayed a docking score of -7.84379 Kcal/mol, higher than ellagic acid's -6.18615 Kcal/mol.

Sustainable technique of dyeing bio-degradable polyester using henna extract

Purpose The purpose of this study is to highlight the threats related to the utilization of synthetic fibers. Volatile organic compounds, particulates and acid gases are released during the production of polyester and other synthetic textiles. Polyester is problematic solid waste material as it takes centuries to break down and hence causes microplastic pollution. Biodegradable synthetic solutions for the replacement of polyester are a sustainable business marketing these days. The naNia fiber is the breakthrough product and it is claimed a biodegradable, compostable and toxin-free polymer. Design/methodology/approach In this research, fabric constructed of naNia fiber was dyed with the extract of naturally occurring Lawsonia inermis (henna) plant leaves. The henna dye was extracted in water and ethanol using different methods, and the better extract was selected by the evaluation of ultraviolet-visible spectroscopy and phytochemical analysis. Henna with ethanol extract showed more desirable results hence it was selected to dye naNia fabric. To improve dyeability, premordanting, simultaneous mordanting and postmordanting were done using chitosan, fresh lemon extract and tannic acid, respectively. The dyed fabric samples were subjected to color strength analysis and multiple colorfastness tests. Findings The colorfastness test has shown good to excellent results. Scanning electron microscope analysis had also shown the attachment of dye molecules to the filaments. This study revealed that henna dye is appropriate to color naNia fiber even without the aid of a mordant. Originality/value For the first time, toxicant-free, biodegradable polyester (naNia) is successfully dyed with sustainable and naturally available dyes and mordants.

Manfaat ekstrak etanol daun inai (lawsonia innermis l.) Sebagai bahan pewarna alami untuk kontrol plak (kajian pada tikus sprague dawley)

Plaque is a soft, colorless deposit containing bacteria that adheres to the surface of the teeth and surrounds the gingiva and other hard surfaces in the oral cavity. A disclosing agent is a selective dye that can detect the presence of dental plaque by changing the color of the plaque so that it contrasts with the tooth surface. Henna has many properties and can produce staining. Lawsone is the main ingredient in the henna plant. Lawsone has a high affinity for binding to proteins and causing staining. The aim is to determine the potential of henna leaf extract (L. inermis L.) as a natural disclosing agent. Laboratories carried out experimental research using rats as experimental animals. The rats were divided into seven groups: control, henna leaf ethanol extract (100%), 50% (25%) group, 12.5% (6.25%), and 3.125% (3.5%) group. The henna leaf ethanol extract group with a concentration of 50% that was left for 30 seconds produced a color close to the positive control used, namely the disclosing agent. The ethanol extract of henna leaves with a concentration of 50%, which was left for 30 seconds, produced a brown to grayish brown color on rat dental plaque and has the potential to be a natural disclosing agent.

Preparation of a nano insecticide using dried Lawasonia Inermis leaves with TeO2 and investigate its effectiveness against house flie

Background: Lawasonia Inermis (henna) extract and trillium dioxide (TeO2) has many applications among them is biomedicine and pharmaceutical industry. Objective: the current study aimed to prepare extract from henna leaves, and synthesize novel nanoparticles derived from henna extract and TeO2, characterized them and test its bioactivity against house flies. Materials and Methods: Lawasonia Inermis (henna) leaves and TeO2 were collected from local markets in Baghdad, Iraq. The extraction of henna leaves was prepared and the TeO2nano particles was synthesized according to a certain procedures, the nanoparticles of the combination of henna extract and TeO2 was prepared a characterized successfully using different techniques such UV-Vis, XRD, AFM. The bioactivity of all the compounds was evaluated and tested. Results: The UV-Vis, XRD and AFM spectra was collected and evaluated. The bioactivity was also examined against house fly and the collected data was clear and very optimistic. It was found that the highest effect was of trillium nanoparticles prepared at a concentration of 500ppm to reach 100% to kill the 1st larval. It also shows clear decreases in the effect at the same concentration above for the 1st larval stage when using only trillium oxide or only henna leaves extract, 30% and 10% respectively, Conclusions: Many conclusions were found in this work, among them are; extract of Lawasonia Inermis leaves was prepared successfully, novel nanoparticles were synthesized from henna extract and TeO2 nanoparticles (HennaTeO2NPs) was prepared, characterization of the novel hennaTeO2NPs was carried out, and the bioactivity of the novel nanoparticles was carried out against house fly and it was noticed that the novel henna nanoparticles shows best activity.

Phenolic Composition and Wound Healing Potential Assessment of Moroccan Henna (Lawsonia inermis) Aqueous Extracts

The present study aims at valorizing Moroccan Henna (Lawsonia inermis) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical analysis of Henna leaves was performed by determining phenolic compound contents, and flavonoids and tannins in it, through its aqueous extracts. Then, specific formulations were prepared using aqueous extracts of L. inermis to assess their in vivo wound healing potential in Swiss albino mice used as animal models. Results disclosed that phenolic compounds (13.48%), as well as flavonoid (9.25%) and tannin (2.57%) contents are higher in Henna leaf extracts from Alnif, while Tazarine Henna aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Sephadex G50 gel corroborates the obtained results and shows that Lawsone levels (Henna coloring agent) are higher in Henna collected from Alnif. Aqueous Henna leaf extracts, at a dose of 10% in petroleum jelly, have been assessed for their ability to heal induced burns in mice. Healing monitoring, carried-out with Henna extracts on mice batches and those of two control batches (mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% (flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the 3 studied Henna-based formulations. These findings suggest the interest of potential development of Henna-based formulations, as a source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.

INVESTIGATION OF THE EXTRACTION PROCESS AND PRELIMINARY PHYTOCHEMICAL ANALYSIS FROM HENNA LEAVES (LAWSONIA INERMIS L.)

Background: Henna (Lawsonia inermis L.), also called Henna tree is a flowering plant used since antiquity to dye skin, hair, fingernails, leather, and wool for thousands of years. Henna are also used to treat injuries, bleeding, blood stasis, irregular menstrual periods. In addition, Henna are also used to treat boils, scabies and poisonous insect bites. With many uses from Henna, it is necessary to prepare solid extracts from medicinal herbs. Objective: To survey on the extraction process and preliminary phytochemical analysis from Henna leaves. Methods: Observation of the influence of extraction solvent (distilled water, 20% alcohol, 40% alcohol), the ratio of medicinal herbs and solvent (1:12; 1:14; 1:16), extraction time (30 min, 45 min, 60 min), the number of times of extraction (1 time, 2 times, 3 times) and extraction temperature (60°C, 70°C, 80°C) to the higher the extraction yield and preliminary phytochemical analysis of Henna extract. Results: It was determined that the extraction solvent was distilled water, the ratio of medicinal herbs and solvent was 1:12, the extraction time was 45 minutes, the number of times of extraction was 2 times and the extraction temperature was 60oC. Henna leaves extract contained groups of compounds such as anthraquinones, hydrolysed triterpenoids, polyphenols and flavonoides. Conclusions: determined the extraction process and groups of compounds of Henna leaves.

The effect of a topical formulation from Lawsonia inermis L. (henna) on pain intensity in patients with chronic sciatica: A randomized double-blind clinical trial

Ethnopharmacological relevanceChronic sciatica (CS) is a common condition of disability and pain. Lawsonia inermis L. (henna) is a medicinal plant that is commonly recommended in traditional Persian medicine textbooks for pain relief in patients with sciatica, particularly in the form of oil. Aim of the studyThis research was designed to investigate the efficacy of a topical formulation from henna on pain intensity in patients with CS. Methods and materialsIn a randomized, double-blind clinical study, 81 patients were randomly allocated to three groups to receive the topical henna formulation (aqueous extract of henna in sesame oil), sesame oil, or placebo, three times daily, for four weeks. The patients were assessed by visual analog scale (VAS), 36-item short form health survey (SF-36), and Oswestry disability index (ODI). ResultsThere was a significant decrease in mean VAS in henna oil compared to sesame oil (p = 0.004) and placebo (p = 0.004). Significant improvements in total SF-36 scores were observed in henna oil and sesame oil compared to placebo (p = 0.011 and p = 0.025, respectively). Furthermore, ODI significantly decreased in henna oil compared to sesame oil (p < 0.001) and placebo (p = 0.005). ConclusionHenna oil seems to be an effective treatment to reduce pain intensity in patients with CS. Moreover, it improves quality of life and functional ability. Further randomized controlled trials with longer intervention periods are recommended to confirm this efficacy.

EVALUATION OF COLOR FASTNESS AND SKIN IRRITATION OF HAIR DYE CREAM FROM HENNA LEAVES (LAWSONIA INERMIS L.)

Objectives: This study aims to evaluate the color fastness and study the skin irritation of a natural hair dye cream from Henna leaves. Materials and method: Nineteen experimental (F1-F19) are conducted using the D-optimal model to evaluate the cause-effect relations and evaluate the color fastness of hair dye cream. Three independent variables were selected for the survey namely Henna extract concentration (X1 includes 5%, 10%, 15%), pH of cream (X2 includes 6, 7, 8), hydrogen peroxide concentration (X3 includes 0%, 2%, 4%). Three dependent variables include color fastness after 1 washes (Y1), 6 washes (Y2) and 12 washes (Y3). Test for skin irritation hair dye cream was applied for rabbits. Results: the optimal parameters of hair dye cream were identified, which included Henna extract concentration of 10%, pH of 6, hydrogen peroxide concentration of 4%. The fastness property of the dye was observed to be very good even after washing 12 times and the absence of sensitivity or reaction on the skin. Conclusion: The results showed that the product has the ability to dye hair and is safe for the skin.

Influence of Henna Extracts on Static and Dynamic Adsorption of Sodium Dodecyl Sulfate and Residual Oil Recovery from Quartz Sand.

The application of surfactant flooding for enhanced oil recovery (EOR) promotes hydrocarbon recovery through reduction of oil-water interfacial tension and alteration of oil-wet rock wettability into the water-wet state. Unfortunately, surfactant depletion in porous media, due to surfactant molecule adsorption and retention, adversely affects oil recovery, thus increasing the cost of the surfactant flooding process. Chemical-based materials are normally used as inhibitors or sacrificial agents to minimize surfactant adsorption, but they are quite expensive and not environmentally friendly. Plant-based materials (henna extracts) are far more sustainable because they are obtained from natural sources. However, there is limited research on the application of henna extracts as inhibitors to reduce dynamic adsorption of the surfactant in porous media and improve oil recovery from such media. Thus, henna extracts were introduced as an eco-friendly and low-cost sacrificial agent for minimizing the static and dynamic adsorption of sodium dodecyl sulfate (SDS) onto quartz sand in this study. Results showed that the extent of surfactant adsorption was inversely proportional to the henna extract concentration, and the adsorption of the henna extract onto the quartz surface was a multilayer adsorption that followed the Freundlich isotherm model. Precisely, the henna extract adsorption on quartz sand is in the range of 3.12-4.48 mg/g (for static adsorption) and 5.49-6.73 mg/g (for dynamic adsorption), whereas the SDS adsorption on quartz sand was obtained as 2.11 and 4.79 mg/g at static and dynamic conditions, respectively. In the presence of 8000 mg/L henna extract, SDS static and dynamic adsorption was significantly reduced by 64 and 82%, respectively. At the same conditions, the residual oil recovery increased by 9.2% over normal surfactant flooding. The study suggests that the use of henna extracts as a sacrificial agent during SDS flooding could result in the reduction of static and dynamic adsorption of surfactant molecules on quartz sand, thus promoting hydrocarbon recovery from sandstone formations.

Nanoemulsion formulation of Lawsonia inermis extract and its potential antimicrobial and preservative efficacy against foodborne pathogens

Formulating plant extracts as nanoemulsions would maximize their use in different applications. This work aimed at formulating henna ( Lawsonia inermis ) extract as nanoemulsion (HENE) and studying its antimicrobial activity on some food-borne pathogens in comparison to the bulk henna extract (HE). The active compounds in henna leaves were extracted by cold solvent extraction method using ethanol and was analyzed using high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and UV–visible spectrophotometry (UV–Vis). It was found that the extract is rich in phenolic compounds, mainly, catechin, methyl gallat, elagic acid and coumaric acid. The ultrasonic emulsification was used to prepare HENE with average droplet size of 90 nm as measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The antimicrobial activities of HENE on seven pathogenic bacteria ( Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa and Yersinia enterocolitica ) and seven fungi ( Trichoderma reesei, Aspergillus terreus, Penicillium digitatum, Aspergillus niger, Candida albicans, Aspergillus flavus and Saccharomyces cerevisiae ) were compared with HE and penicillin. The preservative efficacy of HENE at 0.03% and 0.05% v/v on yoghurt as a model system over a cold storage period of 15 days was studied. HENE showed higher antibacterial efficacy (P < 0.05) against B. cereus, E. coli and P. aeruginosa . The antifungal effect of HENE exceeded that of both penicillin and HE (P < 0.05), in particular, against P. digitatum, S. cerevisiae and A. terreus (P < 0.001). During storage period of yoghurt, no mold or yeast were detected. • Cold extraction of active compounds of Lawsonia Inermis. • Nanoemulsion formulation of Lawsonia Inermis extract. • Antimicrobial activity of Lawsonia Inermis extract nanoemulsion. • Lawsonia Inermis extract nanoemulsion as natural preservative.

Effect of Humic Acid and Henna Extract Lawsonia inermis on Dubas Bug Ommatissus binotatus on Date Palm Trees in the South of Iraq

Al-Saedi, G.F. 2023. Effect of Humic Acid and Henna Extract Lawsonia inermis on Dubas Bug Ommatissus binotatus on Date Palm Trees in the South of Iraq. Arab Journal of Plant Protection, 41(1): 58-64. https://doi.org/10.22268/AJPP-41.1.058064 Date palm is infected by many harmful insect pests, which lead to significant economic losses, among these pests is the dubas bug, Ommatissus binotatus (De Berg). This study was conducted to determine the effect of adding humic acid to NPK fertilizer and the use of henna extract Lawsonia inermis to reduce the effect of dubas bug on date palm trees. The spring insect infestation began at the beginning of March and reached its peak during April of 30.50% insect/leaf of untreated trees, 25% for trees treated with humic acid and henna extract, and 27.30% for trees treated with henna extract only. As for its autumn activity, it started at the beginning of October, and the peak infestation reached 25.60% for untreated trees, 18.70% for trees treated with humic acid and henna extract, and 20.60% for trees treated with henna extract only. The results also showed that trees treated with humic acid and henna extract were less infested with dubas bug, reaching 5.52%, with a significant difference from untreated trees, which amounted to 7.88%. The results also showed that treating infested leaves with dubas bug with humic acid increased the leaf content of total chlorophyll, wax and phenolic compounds compared with untreated leaves infested with dubas bug. Keywords: Dubas bug, humic acid, Lawsonia inermis, date palm, phenolic substances

Potential of a methanolic extract of Lawsonia inermis (L.) leaf as an alternative sanitiser in the time of COVID-19 Pandemic and beyond

To harness the antimicrobial properties of a crude methanolic extract of Henna (Lawsonia inermis) leaf as a potential alternative sanitiser, there is the need to test its performance in different solutions. In this work, the effects of distilled water (dH20), Acetate-HCL (AH) Buffer (pH 4.6), Phosphate Buffer Saline (PBS) (pH 7.2) and Tris-HCL (TBH) Buffer (pH 8.6) on the antibacterial and antiviral activity of the extract were assessed. Through standard phytochemical screening and HPLC-MS (LCMS STANDARD 7.M), it was found that the extract consisted of about 30 different compounds including flavonoids. The extent of the antimicrobial activity of the extract in solutions was in the increasing order of AH > dH2O >>>> TBH > PBS. Under the same conditions, reduced antibacterial activity and complete cessation of the antiviral activity of the extract in TBH and PBS was observed. However, in AH and dH20, within 1–5 min, 1 mg ml−1, 0.125 mg ml−1 and 0.0625 mg ml−1 of the extract caused complete inactivation of E.coli (reductions of 8.2 log CFU ml−1), B. subtilis (reductions of 8.2 log CFU ml−1) and MS2 (reductions of 9.7 log PFU ml−1) respectively. The fluorescence microscopy images of the live/dead staining of the inactivated bacterial samples validated the extent of the inactivation. The broad spectrum and high antimicrobial activity of the extract, coupled with the plant not a staple food, has long history of safe use by humans as a medicine and cosmetic, cheaply available in abundance in many regions of the world, thus making the extract a potential candidate as an alternative sanitiser in the time of COVID-19 Pandemic and beyond.

Effect of aqueous extract Lawsonia inermis leaves on Urea, Creatinine and Histological of kidneys in white male Rats exposed to oxidative stress of H2O2

This study was designed to examine, the effect of Effective aqueous extract Lawsonia inermis leaves as antioxidant on concentration of Urea ,Criatinine and Histological of kidneys in Albino male Rats exposed to hydrogen peroxide induced oxidative stress. This study showed that 0.5% hydrogen peroxide concentration leds to significant increase (P&lt;0.05( in concentration of serum urea and creatinine comparing with control group. While the aqueous extract of Lawsonia inermis concentration (50mg/kg) led to significant decrease (P&lt;0.05) in concentration of serum urea and creatinine compare with control group. Also the results showed no significant increase in concentration of urea and creatinine in group exposed to oxidative stress and aqueous extract of Henna comparing with control group. The histological results of the kidney treated with (0.5%) H2o2 showed degeneration, congestion, lymphatic infilteration, vacuolation in the renal tubules with necrosis comparing with control. While sections of kidney treated with aqueous extract of Henna showed normal appearance of the renal tubules. Histological investigation of kidneys rats treated with H2O2 and extract of Henna showed improvement in histological picture with reduction in infilteration, vacuolation and necrosis but the tissue did not return back to the normal state.

Back to nature: henna extracts from nanotech to environmental biotechnology - a review.

The Lythraceae family includes henna (Lawsonia inermis), which thrives in subtropical and tropical climates. One of its many and long-standing uses is in cosmetics as a pigment to color hair and nails. Additionally, it serves as a disinfectant against microbiological infections and has traditional applications in the textile industry, specifically for coloring wool and nylon. The dried leaves of henna contain a significant amount of lawsone, an active substance bestowing them with staining abilities. Environmental biotechnology, a subfield of biotechnology, engages in the production of biomass or renewable energy sources and the elimination of pollutants, utilizing either entire organisms or their by-products. Recent research indicates that henna, owing to its sustainability, abundant production, simplicity of preparation, low cost, and reputation for being safe and ecologically benign, is exceptionally well-suited to participate in the realm of environmental biotechnology. This review navigates through the most recent studies exploring the use of henna and its extracts for related purposes. These encompass a spectrum of applications, including but not limited to nanobiotechnology, fabric dyeing, corrosion resistance, colored solar cells, carbon dots, and new renewable energy exemplified by biofuel and biohydrogen. Furthermore, henna extracts have been deployed to function as antimicrobials and ward off dangerous insects.

Phytochemical Screening and Chemical Analysis of Freeze-dried Lawsonia inermis Leaves Extract

Lawsonia inermis or Henna is proven to give a natural dye to skin and hair. However, there is still a lack of studies evaluating the properties of Henna in terms of its phytochemical composition. Thus, this study aims to extract henna leaves using the freeze-dried method before testing the phytochemicals in the extract. This study also seeks to analyze the presence of chemicals in the plant extract. The Thin Layer Chromatography, TLC method was used to test the extract's antioxidant, phenolic and polyphenol compounds. Gas Chromatography was applied to analyze the presence of chemicals in the plant extract. This study found that the extract of henna leaves contained antioxidants, phenolic, and polyphenol compounds. There are four chemicals found in the plant sample that show antibacterial, antifungal and antioxidant properties. This study proves that Henna leaves are composed of dye and other valuable compounds that could benefit the body. Hence, the application for this leave could be extended not only for dye purposes but also for others.

Formulation and Effectivity of Henna Leaves (Lawsonia inermis L.) Extract Ointment Against Burn Healing of Rabbit (Orctolagus cuniculus)

Burns are tissue injury resulting from contact with a source of heat. The henna leaves extract (Lawsonia inermis L.) contains tannin, which acts as an astringent to treat wounds. This research seeks to determine the efficacy and quality of henna leaves ointment (Lawsonia inermis L.). The extraction method for henna leaves (Lawsonia inermis L.) entailed maceration with a 96% ethanol solvent. The phytochemical analysis of the 96% ethanol extract of henna leaves revealed the presence of flavonoids, tannins, saponins, and terpenoids. Variable concentrations of henna leaves extract were used to formulate ointments: 2.5% (F1); 5% (F2); 10% (F3); negative control (C-); and bioplacenton® as the positive control. Organoleptic assays, homogeneity, pH, spreadability, and adhesion were used to evaluate the quality of the formulations. In rabbits with inflicted burns, administer ointments F1, F2, F3, C-, and C+ to determine the efficacy of burn treatment. The healing effect is evaluated based on the time it takes for the wound to close (maturation phase), which is characterised by the incision being covered with new tissue. The ointment made from 96% ethanol extract of henna leaves with various concentrations of F1, F2, and F3 met the test requirements for organoleptic, homogeneity, pH, spreadability, and adhesion, according to the results of the ointment quality test. The ANOVA analysis of the effect test revealed that F1, F2, and F3 had a healing effect on wounds. The lesion healing effect of Formula F3 (10%) was not significantly different from the positive control after 11 days