Erythrocyte Hemoglobin Research Articles

Consequences of thimerosal on human erythrocyte hemoglobin: Assessing functional and structural protein changes induced by an organic mercury compound

BackgroundThimerosal (TM) is an organic mercury compound used as a preservative in many pharmacological inputs. Mercury toxicity is related to structural and functional changes in macromolecules such as hemoglobin (Hb) in erythrocytes (Ery). MethodHuman Hb and Ery were used to evaluate O2 uptake based on the TM concentration, incubation time, and temperature. The influence of TM on the sulfhydryl content, production of reactive oxygen species (ROS), and membrane fragility was also evaluated. Raman spectra and atomic force microscopy (AFM) profiles for Ery in the presence and absence of TM were calculated, and docking studies were performed. ResultsAt 37 °C, with 2.50 μM TM (higher concentration) and after 5 min of incubation in Hb and Ery, we observed a reduction in O2 uptake of up to 50 %, while HgCl2, which was used as a positive control, showed a reduction of at least 62 %. Total thiol assays in the presence of NEM (thiol blocker) quantified the preservation of almost 60 % of free SH in Ery. Based on the Raman spectrum profile from Ery-TM, structural differences in the porphyrinic ring and the membrane lipid content were confirmed. Finally, studies using AFM showed changes in the morphology and biomechanical properties of Ery. Theoretical studies confirmed these experimental results and showed that the cysteine (Cys) residues present in Hb are involved in the binding of TM. ConclusionOur results show that TM binds to human Hb via free Cys residues, causing conformation changes and leading to harmful effects associated with O2 transport.

Influence of the NAT2 gene polymorphic markers on the effectiveness and safety of treatment in patients with newly diagnosed pulmonary tuberculosis based on peripheral red blood cell dynamics

Background. Individual sensitivity to isoniazid in tuberculosis patients is determined by the presence of N-acetyltransferase 2 (NAT2) enzyme gene allelic variants in genome. Evaluation of quantitative and qualitative alterations in peripheral blood can be used for diagnosis, disease severity estimation, or as a clue for estimation of anti-tuberculosis chemotherapy effectiveness and safety.Aim: Find associations between acetylation type and peripheral red blood cell (RBC) dynamics; determine the effect of NAT2 acetylation rate on the effectiveness and safety of treatment in patients with newly identified pulmonary tuberculosis (TB) residing in the Sakha Republic (Yakutia).Methods. This study included 146 patients with various clinical forms of newly diagnosed pulmonary TB. Oral isoniazid, rifampicin, pyrazinamide, and ethambutol were administered patients. Genotyping was performed via real time PCR.Results. Rapid and intermediate acetylators showed an increase in hemoglobin concentrations and RBC erythrocyte hemoglobin content by the end of chemotherapy (P<0.05). Incidence of anemia was lower in intermediate acetylators, compared to rapid or slow acetylators (P=0.013). Negative correlation was established between absolute RBC count and slow acetylation type (P=0.017). Patients with rapid acetylation type showed increased RBC distribution width indexes RDW-CV and RDW-SD (P<0.05).Conclusions. An adequate therapeutic effect was achieved with standard doses of anti-TB medications in patients with intermediate acetylation type. Rapid and slow acetylators required anti-TB medication dose correction. Genotyping for NAT2 gene in patients with pulmonary TB enables clinicians to choose the optimal dose of anti-TB medications, specifically, isoniazid dose.

Clinic and diagnostics of iron deficiency anemia in adolescent girls

This article is devoted to the clinic and diagnosis of iron-deficiency anemia inadolescentschoolgirls, the problems that arise in them and the measures to eliminate them.Most of theanalyzed symptoms in girls with YTT and TTK were clearly expressed and the main ones werecombined together. The total amount of hemoglobin anderythrocytes, hematocrit, reticulocyteformula, average amount and concentration of hemoglobin in erythrocytes were determined.

INFLUENCE OF FEED ADDITIVE "FEED BIOPROTEIN" ON BIOCHEMICAL AND MORPHOLOGICAL BLOOD VALUES IN BROILER CHICKENS

The article presents the results of the influence of the experimental feed additive "Feed bioprotein" on biochemical and morphological blood values of broiler chickens, as well as economic indicators. The positive effect of the application of this feed additive has been noted, which is expressed in a higher average daily gain, a decrease in the conversion of feed and an increase in the safety of the stock of experimental groups in comparison with control groups. An increase in erythrocyte concentrations (by 2.16-4.32%), hemoglobin (2.76-5.86%) and total serum protein (by 3.10-6.25%) was found in experimental groups of chickens.

Estradiol dominance induces hemodilution and mild hematological alterations in mifepristone-treated rats.

We examined that an estradiol-dominant state against progesterone could affect hematological parameters through hemodilution because estradiol is known to increase plasma volume via oncotic pressure. We performed a 2- and 3-week repeated oral dose study with mifepristone, a progesterone receptor antagonist, in female rats and examined erythrocyte counts, hemoglobin, hematocrit, plasma volume, levels of estradiol and progesterone, water intake, and water loss. Mifepristone treatment decreased some hematological parameters mildly and increased plasma volume. There were no remarkable changes in the balance of water intake and water loss through urination. Both estradiol and progesterone levels and the ratio of estradiol to progesterone increased. Therefore, our findings indicate that repeated mifepristone treatment increases estradiol levels and plasma volume, resulting in lower erythrocyte counts, hemoglobin, and hematocrit. The present study proved the possible contribution of estradiol to understanding the toxicological significance of mifepristone-induced hemodilution.

Toxicity assessment of beta-cyfluthrin with impact on haematology of fish, Channa punctatus

Âeta-cyfluthrin, a synthetic pyrethroid pesticide, is commonly used in agriculture for pest control and as household insecticide. It is relatively safe to mammals and birds but highly toxic to non-target aquatic organisms including fish. The 96- hour LC50 was determined using standard toxicity assessment tests. Alterations in haematological parameters were recorded after exposure to two sublethal concentrations of beta-cyfluthrin for a period of 15 and 30 days.The results showed a significant decrease in total erythrocyte count, haemoglobin concentration, haematocrit percentage and increase in total leucocyte count in exposed groups.The results of present study indicate a significant toxicological impact of beta-cyfluthrinon in Channa punctatus with marked alterations in haematological parameters.

Eritrograma e estresse oxidativo em bovinos confinados alimentados com feno de Brachiaria sp. e suplementados com antioxidantes

Abstract Brachiaria sp contains sporidesmin that can be oxidized by lipoperoxidation and cause oxidative stress. In the present study we evaluated the effects of different antioxidants on lipoperoxidation of erythrocytes from Nelore cattle fed with Brachiaria sp hay. The experimental design was entirely randomized, in which 40 whole male cattle were divided into five treatments (G1: control - no supplementation; G2: selenium and vitamin E supplementation; G3: zinc supplementation; G4: selenium supplementation and G5: vitamin E supplementation) and allocated in feedlot pens for 105 days. The samples heparinized and withethylenediaminetetraacetic acid (EDTA) were obtained every 28 days for hematological and oxidative stress evaluation (0, 28 56, 84 and 105 days). In the erythrogram total erythrocyte count, hemoglobin, and hematocrit (Ht) were measured. For the evaluation of oxidative stress, in order to analyze the characteristics of the erythrocyte membrane, the thiobarbituric acid reactive substances (TBARS), total glutathione (GSH-T), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) were determined. The results showed that regardless of the treatment there was no oxidative stress during the experimental confinement period and that the joint association of selenium and vitamin E in the bovine diet provided a lower incidence of deleterious alterations on erythrocytes.

Ordered Motions in the Nitric-Oxide Dioxygenase Mechanism of Flavohemoglobin and Assorted Globins with Tightly Coupled Reductases.

Nitric-oxide dioxygenases (NODs) activate and combine O2 with NO to form nitrate. A variety of oxygen-binding hemoglobins with associated partner reductases or electron donors function as enzymatic NODs. Kinetic and structural investigations of the archetypal two-domain microbial flavohemoglobin-NOD have illuminated an allosteric mechanism that employs selective tunnels for O2 and NO, gates for NO and nitrate, transient O2 association with ferric heme, and an O2 and NO-triggered, ferric heme spin crossover-driven, motion-controlled, and dipole-regulated electron-transfer switch. The proposed mechanism facilitates radical-radical coupling of ferric-superoxide with NO to form nitrate while preventing suicidal ferrous-NO formation. Diverse globins display the structural and functional motifs necessary for a similar allosteric NOD mechanism. In silico docking simulations reveal monomeric erythrocyte hemoglobin alpha-chain and beta-chain intrinsically matched and tightly coupled with NADH-cytochrome b5 oxidoreductase and NADPH-cytochrome P450 oxidoreductase, respectively, forming membrane-bound flavohemoglobin-like mammalian NODs. The neuroprotective neuroglobin manifests a potential NOD role in a close-fitting ternary complex with membrane-bound NADH-cytochrome b5 oxidoreductase and cytochrome b5. Cytoglobin interfaces weakly with cytochrome b5 for O2 and NO-regulated electron-transfer and coupled NOD activity. The mechanistic model also provides insight into the evolution of O2binding cooperativity in hemoglobin and a basis for the discovery of allosteric NOD inhibitors.

Dynamics of Morphological Indicators of Blood of Piglets under the Influence of Iron Clathrochelate Complex and Cyanocobalamin

Iron deficiency anemia is one of the most common non-contagious diseases of piglets. Veterinary antianemic drugs have several drawbacks, so finding new medicines is an important current task for scientists. Therefore, the present study investigated the antianemic effect of iron (IV) clathrochelate in the organism of piglets. The subsequent studies included the exploration of its antianemic actions, particularly in combination with cyanocobalamin when this combination was administered to sows for prophylaxis in piglets. The experiment was carried out on 30 suckling piglets during the period of their detention with sows. According to the method of analogue groups, two groups of control (I) and experimental (II, each containing 15 animals) were formed and they were studied for 30 days. The piglets from five sows (three from each) were selected for the experimental group. During the pregnancy of these sows, 10 ml of 10% solution of iron (IV) IV clathrochelate and solution of cyanocobalamin were injected intramuscularly twice 7 and 14 days before their expected farrowing. For prevention of iron deficiency anemia, the traditional solution of iron dextran was administered once intramuscularly to piglets of the control group. The investigative material included the blood samples of piglets considering the dynamics of probable changes in the number of erythrocytes, hemoglobin content and hematocrit, and other morphological indicators and blood indices of piglets. The dynamics of changes in erythrocyte count, hemoglobin content, hematocrit, leukocytes and platelets, indices of blood almost did not differ from the dynamics of these values when using only 10% solution of iron (IV) clathrochelate for pregnant sows. The proposed scheme for the prophylaxis of iron deficiency anemia in piglets, involving simultaneous intramuscular injections of IV clathrochelate and cyanocobalamin to pregnant sows, is somewhat inferior to the previous preventive measures, which included only the intramuscular injections of iron (IV) IV clathrochelate, but it can be recommended as highly effective.

Systemic Effects of Photobiomodulation on Blood Components in the Treatment of Community-Acquired Pneumonia.

Background: The analysis of the complete blood count (CBC) of patients with community-acquired pneumonia (CAP) is an essential practice both for diagnosing the disease and for evaluating the patient's clinical evolution. It is proposed in the present study to analyze the hematological alterations resulting from photobiostimulation using near-infrared light-emitting diodes (LEDs) in patients with CAP. Methods: This was a clinical, prospective, blinded, and descriptive longitudinal study that involved 21 patients undergoing CAP treatment who were divided into two groups: LED, 11 patients who were treated with infrared LED and conventional treatment; and CON (control), 10 patients who received only conventional treatment (antibiotic therapy and physiotherapy). Physiotherapy was applied before LED irradiation in the LED group. The patients' CBCs were obtained before and after treatment, and erythrocyte counts, hemoglobin and hematocrit concentrations, and leukocyte and platelet counts were assessed. The phototherapy was performed with a vest with an array of 300 LEDs (940 nm) mounted on an area of 36 × 58 cm and positioned in the patient's anterior thoracic and abdominal regions. The total power was 6 W, with 15 min of irradiation time. The patients were treated daily for seven consecutive days. Statistical analyses of the intra- and intergroups of CBC data were done using Student's t-test and one-way ANOVA (analysis of variance), respectively, both at the significance level of α = 0.05. Results: There was a statistically significant recovery difference after treatment in the LED group compared with the CON group for erythrocytes, hemoglobin, leukocytes, segmented and band neutrophils, lymphocytes, and monocytes (p < 0.05). The greatest differences between the LED and CON groups were lymphocyte count reduction (60% vs. 16%), erythrocyte increase (86% vs. 35%), and leukocyte reduction (28% vs. 15%). Conclusions: The hematologic components of CAP patients recovered their normal values faster with conventional treatment associated with infrared LED therapy, thus indicating greater treatment efficiency when compared with the conventional therapy. This study was registered with the Brazilian Registry of Clinical Trials (ReBeC) under Universal Trial Number (UTN) U1111-1229-1296 (2019/06/05).

Polymorphisms in the ovine GP5 gene associated with blood physiological indices

This study aimed to analyze the effects of polymorphisms in GP5 on blood physiological indices of 1065 sheep. The coefficients of variation of the red blood cell count (RBC), hemoglobin concentration (HGB), mean platelet volume (MPV), mean erythrocyte hemoglobin content (MCH), and red blood cell distribution-coefficient of variation (RDW-CV) were greater than 10%, and there was a very significant correlation between the main indices such as RBC, white blood cell, and platelet count (PLT) and most other indices (p < 0.01). qRT-PCR showed that GP5 was expressed in the heart, liver, spleen, lung, kidney, rumen, duodenum, muscle, tail fat, and lymph tissue, with significantly higher expression in the lymph. Subsequently, we detected single nucleotide polymorphisms (SNPs) in GP5 from group, which identified synonymous mutation g.657 T > C in the first exon of GP5. Association analysis showed significant correlations between the SNP and the physiological traits (p < 0.05), in which the RBC, neutrophilic granulocyte (NEUT) and RDW-CV values in sheep with the TC genotype and TT genotype were markedly lower than those in the CC genotype (p < 0.05). Thus, GP5 polymorphisms could be candidate biomarkers to screen blood physiological indices.

Evaluation of Liver Function and Haemorheological Parameters in Some Carpenters Exposed to Wood Dust in Port Harcourt

Aim: To evaluate liver function and haemorheological parameters in some carpenters exposed to wood dust in Port Harcourt.&#x0D; Methodology: This cross-sectional study was carried out in some parts of Port Harcourt, the capital city of Rivers State in the southern part of Nigeria. The locations of the research included Sandfield mile 2 Diobu, Marine base, and Abuja by-pass Nkpolu-oroworukwo, Port Harcourt. Ethical approval and informed consent of the participants were obtained before commencement of the study. A total of one hundred and fifty (150) apparently healthy subjects aged between 20 t0 50 years were used for the study. Ninety (90) carpenters exposed to wood dust served as test subjects, while sixty (60) non- carpenters, non-wood workers not exposed to wood dust served as controls. Interviewer based sample questionnaire was administered to the subjects. Liver function parameters, Packed cell volume (PCV), erythrocyte sedimentation rate (ESR), haemoglobin concentration (Hb), and fibrinogen were analysed using standard laboratory techniques.&#x0D; Results: The mean age of the test subjects (36.5 ± 9.9 years) was not significantly different (P &gt; .05) from that of the controls (32.1 ± 5.8 years). There were no significant differences (P &gt; .05) in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), Total protein (TP), albumin (ALB), total bilirubin (TB), conjugated bilirubin (CB), PCV and Hb in the test subjects compared to the controls. ESR (19.60 ± 10.67mm/Hr) and fibrinogen (624 ± 60.88ng/mL) were significantly higher (P&lt; .05) in the tests (carpenters) compared to ESR (8.41 ± 5.36mm/Hr) and fibrinogen (448.4 ± 67.91ng/mL) in the controls.&#x0D; Conclusion: Wood dust exposure did not alter the liver function, PCV, and Hb levels of carpenters. However, with increases in ESR and fibrinogen in the carpenters, there may be a risk of inflammation to wood dust exposure in the carpenters, with negative consequences to the vasculature.

Peroxidase activity of erythrocytes hemoglobin under action of low-frequency vibration

Background. Hemoglobin is a hemoprotein which in the presence of oxidative equivalents, such as H2O2, can act as peroxidase with a very high oxidative potential. Hemoglobin oxidation is accompanied by generation of highly oxidized forms of iron and globin radicals that have high oxidative activity and are toxic to cells. In addition, peroxidase activity may indicate structural changes that occur in the hemoglobin molecule as a result of chemical modification. Materials and Methods. Erythrocyte suspension was subjected to vibration for 3 h within the frequency range from 8 to 32 Hz with amplitudes of 0.5 ± 0.04 and 0.9 ± 0.08 mm. At certain intervals, hemoglobin peroxidase activity was determined together with the content of its ligand forms in the hemolysates of cells. Additionally, experiments were performed to investigate the mechanism and calculate the kinetic parameters of peroxidase reaction. Results and Discussion. Experimental data on low-frequency vibrations effect on erythrocyte hemoglobin peroxidase activity were analyzed. The kinetics of the oxidation reaction of p-phenylenediamine by hemoglobin in erythrocytes was studied. It was found that peroxidase oxidation has a ping-pong mechanism. The kinetic parameters of the peroxidase reaction involving hemoglobin were determined. The change of kinetic parameters after two-hour exposure to the incubation medium and low-frequency vibration was studied. A possible mechanism of action of hemoglobin in oxidation reactions involving H2O2 was proposed. Conclusion. Any effect that initiates the formation of methemoglobin leads to an increase in the peroxidase activity of hemoglobin due to the involvement of the latter in the pseudoperoxidase cycle and the formation of toxic reactive globin radicals. The high content of oxyhemoglobin in the cell, observed under vibrations within the frequency range of 16–32 Hz with an amplitude of 0.9 ± 0.08 mm, can prevent its oxidation and involvement in the pseudoperoxidase cycle.

Erythrocyte Indices and Long-Term Blood Pressure Variability in Military Males.

Severe microcytic anemia has been associated with BP changes. Whether the erythrocyte indices are associated with long-term BPV is unknown. This study aimed to investigate the association of hemoglobin levels and erythrocyte size with long-term blood pressure variability (BPV) in young males. This study included 1,112 healthy military males, averaging 32 years of age, in Taiwan. All participants took a measurement of systolic and diastolic BP (SBP and DBP) every two-year from 2012 to 2018 (2012-14, 2014-15, 2015-16, 2016-18). Levels of hemoglobin and mean corpuscular volume (MCV) of erythrocytes were obtained at the first visit. Long-term BPV was assessed by the standard deviation (SD) and average real variability (ARV). Multivariate linear regression analysis with adjustment for the baseline BP levels and other covariates was used to elucidate the association. Hemoglobin levels were borderline positively correlated with SD DBP (β and standard errors = 0.016 (0.009), P =0.06). In those with hemoglobin levels of 10.0-13.9 g/dL, hemoglobin was negatively correlated with SDSBP (β= -0.039 (0.018), P =0.03). In contrast, MCV levels were borderline positively correlated with SDSBP (β =0.085 (0.052), P =0.09). In those with MCV levels <80 fL, MCV was positively correlated with SDSBP and ARVSBP (β= 0.445 (0.210) and 0.286 (0.149), p = 0.03 and 0.05, respectively). There were inconsistent patterns for the associations of erythrocyte indices with longterm BPV. We found a U-shaped relationship of hemoglobin levels with systolic BPV, whereas there was a positive linear relationship of hemoglobin and MCV levels with diastolic BPV, respectively.

Erythrocytic Antioxidant Enzymes, Plasma Malondialdehyde and Haemoglobin Levels in Plasmodium Falciparum Infected Malaria Patients in Lagos, Nigeria

This study investigated the effect of malaria parasitaemia on Plasmodium falciparum infected human erythrocytes oxidative stress biomarkers and haemoglobin levels. Seventy (70) human subjects of fifty (50) P. falciparum positive and twenty (20) control subjects between the ages of 10-60 years were selected for this study. Rapid Diagnostic Test (RDT) and microscopy were used to identify P. falciparum. The samples were matched based on age, sex and level of parasitaemia. Samples of blood were collected for the determination of P. falciparum, level of parasitaemia, anti-oxidant assay and haemoglobin levels; to assess the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), malondialdehyde (MDA), total protein (PRO), reduced glutathione (GSH), haemoglobin and Parasite density. Haemoglobin level was determined using a Coulter A-T Pierce haematology analyzer (Beckman Coulter, Inc. Fullerton, CA, USA). This study showed that the mean level of PRO, CAT, MDA and SOD was significantly higher among the P. falciparum positive patients to those in the control while GPx level was lower, also, the mean level of HGB was significantly lower in the P. falciparum positive patients to those in the control. MDA, SOD, GSH and PRO level were higher among age group (10-20) in the P. falciparum infected patients and lower in the control subjects when compared to other age groups. MDA, SOD and PRO level were higher in the males than the females in both the malaria positive and controls. This study indicates that high parasitaemic patients are at greater risk of anaemia and oxidative stress compared to low parasitaemic ones.

Safety of torasemide in healthy adult dogs administered daily for 26weeks.

Thirty‐two (16 males and 16 females) healthy young beagles were randomly divided into four groups of eight. The control group remained untreated. Torasemide (ISEMID®, Ceva Santé Animale) was orally administered, once daily, at 0.5 mg/kg from Days 1–5 then 0.25 mg/kg to Day 182, and at three times and five times this dosing regimen in two additional groups. Treated animals (predominantly at the higher dose levels) showed dryness of the oral mucosa, evidence of diuresis, decreased diet consumption, decreased bodyweight gain over the first 3 weeks, increased water consumption, increases in erythrocytes count, haemoglobin, calcium and magnesium, decrease in chloride, phosphorus, potassium and sodium, increases in urine pH, decreases in urine specific gravity and increases in serum aldosterone concentrations. Plasma concentrations of torasemide increased in a dose‐dependent manner and showed no evidence of accumulation. There were also changes to electrocardiogram patterns and the macroscopic and microscopic appearance of the kidney and adrenal glands, but these changes were almost exclusively confined to the over‐dosed groups. In conclusion, torasemide was found to be safe when administered to dogs at 0.25 mg/kg once daily for 26 weeks, and any changes were consistent with its known diuretic effects.

Hematological, Micro-Rheological, and Metabolic Changes Modulated by Local Ischemic Pre- and Post-Conditioning in Rat Limb Ischemia-Reperfusion.

In trauma and orthopedic surgery, limb ischemia-reperfusion (I/R) remains a great challenge. The effect of preventive protocols, including surgical conditioning approaches, is still controversial. We aimed to examine the effects of local ischemic pre-conditioning (PreC) and post-conditioning (PostC) on limb I/R. Anesthetized rats were randomized into sham-operated (control), I/R (120-min limb ischemia with tourniquet), PreC, or PostC groups (3 × 10-min tourniquet ischemia, 10-min reperfusion intervals). Blood samples were taken before and just after the ischemia, and on the first postoperative week for testing hematological, micro-rheological (erythrocyte deformability and aggregation), and metabolic parameters. Histological samples were also taken. Erythrocyte count, hemoglobin, and hematocrit values decreased, while after a temporary decrease, platelet count increased in I/R groups. Erythrocyte deformability impairment and aggregation enhancement were seen after ischemia, more obviously in the PreC group, and less in PostC. Blood pH decreased in all I/R groups. The elevation of creatinine and lactate concentration was the largest in PostC group. Histology did not reveal important differences. In conclusion, limb I/R caused micro-rheological impairment with hematological and metabolic changes. Ischemic pre- and post-conditioning had additive changes in various manners. Post-conditioning showed better micro-rheological effects. However, by these parameters it cannot be decided which protocol is better.

Assessment of the prevalence of anemia in patients with primary hyperparathyroidism: a single-center observational study

The combination of primary hyperparathyroidism (PHPT) with anemia was first described in 1931. It remains unclear whether PHPT is the direct cause of anemia, or it develops due to PHPT's complications. The frequency of PHPT--associated anemia in the Russian population is unknown. To assess the prevalence of anemia in patients with PHPT admitted to the Department of Parathyroid Glands Pathology in the Endocrinology Research Centre from January 2017 to August 2020. The study included patients with PHPT over 18 years old. A single-center observational one-stage one-sample uncontrolled study was carried out. We analyzed laboratory and instrumental data obtained during inpatient examination in accordance with the standards of medical care. Statistical analysis was performed using Statistica 13 (StatSoft, USA) and SPSS (IBM, USA) software packages. The study included 327 patients with PHPT, 28 (9%) men and 299 (91%) women. The median age was 59 years [51; 66]. 26 patients (8%) with anemia were identified. Statistically significant differences between patients with and without anemia were found only in the GFR. Comparison of patients with and without anemia didn't reveal any significant differences in the incidence of PHPT's complications.Significant differences in serum hemoglobin concentration and average hemoglobin concentration in erythrocytes were revealed between patients with and without vertebrae fractures. In the group of patients without compression fractures these parameters were higher.In the subgroup of patients with total calcium concentration above 3 mmol/L and PTH above 3 normal values, the incidence of anemia reached 21% (95% CI: 10%; 35%). Within this group we revealed tendencies to higher levels of PTH, ionized calcium and osteocalcin in patients with anemia. In general, there was no correlation between hypercalcemia, the degree of PTH elevation and the presence of anemia in patients with PHPT. However, in the subgroup of patients with severe hypercalcemia, there was a relationship between the concentration of PTH, ionized calcium and the presence of anemia. In patients with PHPT and vertebral fractures, significantly lower concentrations of blood hemoglobin and hemoglobin in erythrocytes were observed.

Effects of bisphenol a on hematological, serum biochemical, and histopathological biomarkers in bighead carp (Aristichthys nobilis) under long-term exposure.

Bisphenol A (BPA) is one of the highest volume chemicals produced in the world and is frequently used in dental sealants, water bottles, food, and beverage packaging. Due to persistent applications, BPA has become a potential threat to a variety of organisms including public health. In this study, a total of 80 bighead carps were randomly placed in different four groups (A-D). Fish in groups B, C, and D were exposed to BPA @500, 1000, and 1500μg/L, respectively for 60days. Fish in group A served as an untreated control group. The body weight was significantly decreased while the absolute and relative weight of different visceral organs increased significantly (p < 0.05) in fish exposed to higher concentration (1500μg/L) of BPA. Results on proximate analysis showed significantly lower values of crude proteins, lipids, and moisture contents while increased contents of ash in muscles of treated fish. The erythrocyte counts, hemoglobin concentration, lymphocytes, and monocytes significantly decreased while total leukocyte and neutrophil counts significantly increased in treated fish. Results exhibited that different serum biochemistry parameters like serum albumin and total proteins decreased significantly (p < 0.05) while alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), urea, creatinine, glucose, cholesterol, and lactate dehydrogenase (LDH) increased significantly (p < 0.05) in treated fish. Histopathological ailments like pyknosis, degeneration of glomeruli, increased Bowman's space, ceroid formation in kidneys while ceroid formation, hemorrhages, pyknosis, karyorrhexis, karyolysis, nuclear hypertrophy, and eccentric nuclei were observed in the liver of treated fish. Histological observation of different sections of the brain of treated fish exhibited degeneration of neurons in the cerebellum, lipofuscin deposition, microgliosis, necrotic neurons, inflammatory cells, and hemorrhage. Results on light microscopic observation of different sections of the heart of bighead carp revealed necrosis, inflammatory reaction, neutrophilic myocarditis, and hemorrhages. In conclusion, it is suggested that BPA induces adverse effects on physical, blood-biochemical parameters, and histopathological changes in multiple visceral tissues of exposed fish.

Hemogen/BRG1 Cooperativity Is Required for Erythrocyte Maturation and Hemoglobin Production during Mammalian Erythropoiesis

Hemogen, also known as EDAG, is a hematopoietic tissue-specific gene that regulates the proliferation and differentiation of hematopoietic cells. However, the mechanism underlying hemogen function in erythropoiesis is unclear. We found that depletion of hemogen in human CD34 + erythroid progenitor cells and HUDEP2 cells significantly reduced the expression of genes associated with heme and hemoglobin synthesis, supporting a positive role of hemogen in erythroid maturation. In human K562 cells, hemogen antagonized the occupancy of co-repressors NuRD complex and facilitated LDB1 complex-mediated chromatin looping. Hemogen recruited SWI/SNF complex ATPase BRG1 as a co-activator to regulate nucleosome accessibility and H3K27ac enrichment for promoter and enhancer activity. To ask if hemogen/BRG1 cooperativity is conserved in mammalian systems, we generated hemogen KO/KI mice and investigated hemogen/BRG1 function in murine erythropoiesis. Loss of hemogen in E12.5-E16.5 impeded erythroid differentiation through reducing the production of mature erythroblasts. ChIP-seq in WT and hemogen KO animal revealed BRG1 is largely dependent on hemogen to occupy chromatin at erythroid gene promoters and enhancers. In summary, hemogen/BRG1 interaction in mammals is essential for erythroid maturation and hemoglobin production through its active role in promoter and enhancer activity and chromatin organization. DisclosuresNo relevant conflicts of interest to declare.