Blood Hemoglobin Concentration Research Articles

Evaluation of Blood Pressure, Liver Function, and Hemoglobin Concentration Alterations in Cigarette Smokers on the West Coast of Libya

Background: Cigarette smoking is one of the 10 greatest contributors to global death and disease. Cigarette smoking is the most common type of tobacco use. In average, to date 47.5% of men and 10.3% of women are current smokers. Tobacco continues to be the second major cause of death in the world. It consists of many chemicals, including cytotoxic, carcinogenic and free radicals, therefore it affects many organs if not all. Objective: The aim of this study is to assess the extent of adverse effect of cigarette smoking on blood pressure, liver function, and hemoglobin concentration in male population on the West Coast of Libya. Methods: This study was conducted on (200) healthy male subjects, their ages ranged from 15 to 85 years. They were divided into four groups; 50 non-smokers as control group (age between 15-35 years), 50 smokers (age between 15-35 years), 50 non-smokers as control group (age between 36-84 years) and 50 smokers (age between 36-84 years). The blood was collected in EDTA tubes to test the Complete blood count (CBC) and in plain tubes for biochemical assay (activities of liver enzymes). The Blood pressure of subjects was measured directly after sample collection. Result: The blood pressure among the group of age 36-84y was significantly higher (P < 0.05) when compared between the smokers and the nonsmokers group, while among the group of age 15-35 was not significant. The results of liver enzymes activities among the group of age 36-84y showed that ALT and ALP were significantly high (P <0.05) in the smoker group when compared with the nonsmoker group, while serum AST was not significant (P>0.05). However, among the group of age between 15-35 years, the results showed that statistically significant differences (P<0.05) were observed in ALT and AST activities for the smoker group when compared with the nonsmoker group. Regarding RBCs count and hemoglobin concentration among both of groups (age between 15-35 & 36-84 years), the results showed that a significant difference in mean RBCs count and hemoglobin concentration were noted between the smokers and the nonsmokers. Conclusion: It can be concluded that exposure to cigarette smoking leads to an increase the blood pressure. Cigarette smoking can lead to an increase in liver enzymes activities, RBCs count and Hb concentration.

Nifuroxazide mitigates doxorubicin-induced cardiovascular injury: Insight into oxidative/NLRP3/GSDMD-mediated pyroptotic signaling modulation

Doxorubicin (DOX) is a widely used powerful anthracycline for treatment of many varieties of malignancies; however its cumulative and dose-dependent cardio-toxicity has been limited its clinical use. In the current study, in vivo and in vitro (neonatal rat's cardiomyocytes) experiments were conducted to identify the impact of nifuroxazide (NIFU) on DOX-induced cardiomyopathy, vascular injury, and hemato-toxcity and plot the underlying regulatory mechanisms. Cardiovascular injury was induced in vivo by I.P. injection of an overall dose of DOX (21 mg/kg) administered (3.5 mg/kg) twice weekly for 21 days. NIFU (10 and 30 mg/kg) was administered orally once daily for 21 days, 1 week after DOX injection initiation. In vivo experiments confirmed NIFU to restore blood cells counts and hemoglobin concentration. Moreover, NIFU normalized the myocardial functional status as confirmed by ECG examination and myocardial injury markers; CK-MB, LDH, and AST. NIFU restored the balance between TAC and both of ROS and MDA and down-regulated the protein expression of TLR4, NF-kB, TXNIP, NLR-family pyrin domain containing 3 (NLRP3), caspase-1, IL-1β, and GSDMD-N terminal, with inhibition of the up-stream of NLRP3 and the down-stream DOX-induced pyroptosis. The in vitro assay confirmed well preserved cardiomyocytes' architecture, amelioration of NLRP3/IL-1 β-mediated cell pyroptosis, enhanced cell viability, and improved spontaneous beating. Moreover, NIFU normalized the disturbed aortic oxidant-antioxidant balance; enhanced eNOS- mediated endothelial relaxation, and down regulated IL-1β expression. Thus, NIFU may be proposed to serve as a cardioprotective agent to attenuate DOX-induced cardio-toxicity and vascular injury.

Haematological Alteration among Patients with Oral and Dental Health Problems in the Tripoli Region

Background: Oral health plays an important role in maintaining life functions and quality of life. Gingivitis and periodontitis are two forms of periodontal disease that exist on a spectrum ranging from bleeding, erythematous, and inflamed gingival tissue to the loss of attachment and alveolar bone. Dental caries and periodontal diseases are the most common oral ailments and the major causes of tooth loss. Objectives: The study aimed to evaluate the haematological alterations among patients with oral and dental health problems in the Tripoli region. Material and Methods: The present study was conducted on 200 patients with oral and dental health problems attending six medical centers in Tripoli region from the 01st March 2022 to the 01st June 2022. Also, 100 healthy individuals without any oral and dental health problems or any other diseases were recruited as a control group. This study was approved by the Research and Ethical Committee of the Libyan Academy of graduate studies and medical centers. One ml of venous blood was withdrawn from each participant in the study for the determination of haematological parameters. The data were compared using SPSS Statistics for Windows, Version 26. Results: The results showed that red blood corpuscles (RBCs) count, hemoglobin concentration, hematocrit value, MCV, MCH, and MCHC in patients with oral and dental health problems were decreased significantly (P<0.01) compared to the control group. White blood cell count, neutrophils%, and platelets count in patients with oral and dental health problems were significantly (P<0.01) increased compared to the control group. On the other hand, lymphocytes% and mixed % WBCs were significantly (P<0.01) decreased compared to the control group. Conclusion: It can be concluded that oral and dental health problems were associated with a significant alterations in haematological parameters. Red blood corpuscles count, hemoglobin concentration, hematocrit value, MCV, MCH, MCHC, lymphocytes%, and mixed % WBCs were decreased significantly and White blood cell count, neutrophils%, and platelets count were significantly increased in patients with oral and dental health problems compared to the control group. Further studies are needed to confirm these results.

Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation

Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs.

Dietary Iron Intake and Biomarkers of Iron Status in Slovenian Population: Results of SI.Menu/Nutrihealth Study.

Inadequate iron intake and iron deficiency are recognised as a public health problem in the population at large, and particularly in specific subpopulations. Dietary iron intake was analysed using data of the national Slovenian food consumption study, SI.Menu (n = 1248 subjects; 10−74 years), while iron status was evaluated with laboratory analyses of blood haemoglobin, serum ferritin, and iron concentration in samples, collected in the Nutrihealth study (n = 280, adults). The estimated daily usual population-weighted mean iron intakes ranged from 16.0 mg in adults and the elderly to 16.7 in adolescents, and were lower in females for all three age groups. The main dietary iron sources in all the age groups were bread and bakery products, meat (products), fruit, and vegetables. The highest prevalence of haemoglobin anaemia was observed in females aged 51−64 years (6.7%). Critically depleted iron stores (ferritin concentration < 15 µg/L) were particularly found in premenopausal females (10.1%). Factors influencing low haemoglobin, ferritin, and iron intake were also investigated. We observed significant correlations between iron status with meat and fish intake, and with iron intake from meat and fish, but not with total iron intake. We can conclude that particularly premenopausal females are the most fragile population in terms of inadequate iron intake and iron deficiency, which should be considered in future research and public health strategies.

The Effects of Exercise Training on Recovery of Biochemical and Hematological Outcomes in Patients Surviving COVID-19: A Randomized Controlled Assessor-Blinded Trial

BackgroundNo previous research has investigated the direct effects of exercise interventions on COVID-19 outcomes. The aim is to investigate the effect of 8 weeks of home-based moderate-intensity continuous training (MICT), resistance training (RT), and combined aerobic and resistance training (CET) on biochemical and hematologic markers associated with COVID-19 symptoms and severity in COVID-19 survivors.MethodsA total of 547 male and female COVID-19 survivors were screened, and 296 (aged 20–93 years) were randomly assigned in a 1:1:1:1 ratio to one of four groups: MICT (n = 74), RT (n = 74), CET (n = 74), or non-exercise (NON-EX, n = 74). Blood samples were taken at baseline, at week 4, and week 8 after training.ResultsAfter the intervention, compared with the NON-EX group, all 3 MICT, RT, and CET interventions caused significant improvements in the levels of creatine kinase (CK), lactate dehydrogenase (LDH), C-reactive protein (CRP), troponin-I, d-dimer, creatinine, urea, potassium (K), sodium (Na), white blood cell (WBC), neutrophils, lymphocytes, red blood cells (RBC), platelets, hemoglobin, and hematocrit concentrations (P < 0.05). CET was effectively superior to MICT and RT in the improvements in the biochemical and hematological variables studied (P < 0.05).ConclusionsOverall, the present study demonstrates that long-term MICT plus RT interventions have a synergistic effect in accelerating and enhancing the recovery in patients surviving COVID-19.Trial registration IRCT20160605028270N3, 6 September 2020.

Amelioration of mercuric chloride-induced physiologic and histopathologic alterations in rats using vitamin E and zinc chloride supplement

The drastic effects of mercuric chloride and the protective efficiency of vitamin E and zinc chloride co-supplementation were clearly investigated in this study. Male rats were divided into four groups. The first was the control. The second received vitamin E (100 mg/kg) and zinc chloride (30 mg/kg) daily. In comparison, the third received mercuric chloride (1 mg/kg) daily, and the fourth received the same mercuric chloride dose supplemented with the same vitamin E and zinc chloride doses. Mercury promotes a significant decline in body weight. It causes a considerable reduction in total red blood cells (RBCs) count and hemoglobin concentration; however, white blood cells (WBCs) increased significantly. Significant mercury-induced elevations in hepatic and renal functions were observed. Mercury induced substantial reductions in catalase (CAT) and superoxide dismutase (SOD). Mercury caused apoptotic DNA fragmentation. It induced degeneration and necrosis in the liver and kidney. It induced necrosis, leukocyte infiltration and blood vessel congestion in the cerebral cortex. Shrinkage and deterioration of Purkinje cells of the cerebellum were observed in response to mercuric chloride toxicity. Mercuric chloride enhanced shrinking in seminiferous tubules and Leydig cells. It reduced sperm count, sperm motility, and testosterone concentration; however, it promoted abnormal sperm morphology. Administration of vitamin E and zinc chloride showed marked improvement in different parameters under investigation, however, further research is needed to determine fate of mercury.

Assessing the genotoxicity of oral zinc oxide nanoparticle administration in male rats using micronuclei and comet assay

Zinc oxide nanoparticles (ZnO-NPs) are regularly utilized in the food and fertilizers industries. In our investigation, rats received oral administration of ZnO NPs with a particle size of 30±5 nm once daily at doses of 100, 200, 300, 400, and 600 mg/kg for ten weeks in order to assess the genotoxic effect. Impacts on hematological markers, genotoxic impact, and growth were investigated. The findings showed that ZnO-NPs significantly reduced body weight gain, red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit value (HCT), and platelet count (PLT), while increasing white blood cell (WBC), mean capsular volume (MCV), mean capsular hemoglobin (MCH), and mean capsular hemoglobin concentration (MCHC) in the treated rats. Our results for the comet assay and micronuclei test show a dosage-dependent increase in DNA fragmentation, which was supported by an increase in the percentage of DNA that is tailed, the length and intensity of DNA tails, and the tail moment, especially at the dose of 600 mg/kg. According to the findings, the frequency of micronucleated cells has increased.

Levels of TNF-α and Soluble TNF Receptors in Normal-Weight, Overweight and Obese Patients with Dilated Non-Ischemic Cardiomyopathy: Does Anti-TNF Therapy Still Have Potential to Be Used in Heart Failure Depending on BMI?

We sought to measure the levels of adipokines, TNF-α and soluble receptors (sTNFr1, sTNFr2) in heart failure patients with reduced ejection fraction (HFrEF) due to non-ischemic cardiomyopathy (nDCM). A total of 123 patients with HFrEF due to nDCM were divided into three groups according to BMI: 34 (27.6%) normal weight, 56 (45.5%) overweight and 33 (26.8%) obese. A six-minute walk test, echocardiography and right heart catheterization were performed. Serum concentrations of adiponectin, leptin, NT-proBNP, blood hemoglobin, sodium, creatinine, ALAT, AspAT, bilirubin, CRP, lipids, TNF-α, sTNFr1 and sTNFr2 receptors were measured. Obese patients had the lowest NT-proBNP concentrations, significantly higher leptin levels and higher leptin/adiponectin ratios. The concentration of sTNFr1 was higher in normal-weight patients. In all groups, TNF-α concentrations correlated positively with sTNFr1 (p &lt; 0.001). Higher levels of sTNFr1 were associated with higher sTNFr2 (p &lt; 0.001) and CRP (p &lt; 0.001). Moreover, the concentration of sTNFr2 positively correlated with CRP (p &lt; 0.05) and adiponectin (p &lt; 0.001). Levels of TNF-α were not associated with elevated CRP. This study demonstrated that changes in the concentrations of TNF and its receptors differ between groups of patients with different BMI. These findings suggest that the effective use of anti-TNF therapy is dependent not only on BMI, but also on concentrations of TNF-α receptors and other laboratory parameters.

Efficacy and Safety of Ropeginterferon Alfa-2b for Pre-Fibrotic Primary Myelofibrosis and DIPSS Low/Intermediate-1 Risk Myelofibrosis

Most patients with early/pre-fibrotic primary myelofibrosis (pre-PMF) and dynamic international prognostic scoring system (DIPSS) low or intermediate-1 risk myelofibrosis (MF) progress to overt PMF or higher risk MF. There is currently no consensus on the optimal treatment strategies in these patients. Ropeginterferon alfa 2b (P1101) is a next generation monopegylated interferon alfa-2b developed specifically to treat myeloproliferative neoplasms (MPN). The P1101MF study is a multicenter phase 2 study of P1101 for patients with pre-PMF and DIPSS low/intermediate-1 risk MF (NCT04988815). Key eligibility included the need for cytoreduction, morphologically confirmed pre-PMF, overt PMF, post-polycythemia vera MF (PPV-MF) or post-essential thrombocythemia MF (PET-MF), and DIPSS low/intermediate-1 risk category. The primary outcome was the clinicohematologic complete response (CHCR) rate at 24 and 48 weeks. Secondary outcomes included adverse events (AEs), changes in allele burden of driver genes and other non-driver mutations, changes in quality-of-life (QOL), changes in cytokine profiles and changes in bone marrow morphology. Patients received P1101 at a starting dose of 250mcg followed by 350mcg at week 2 and 500mcg every 2 weeks from week 4 onwards. At the data cut-off of 28 July 2022, 56 patients (33 men, 23 women) with a median age of 58 (range: 30-87) were enrolled. Thirty eight patients (68%) had pre-PMF, 5 (9%) had overt PMF, 3 (5%) had PPV-MF and 10 (18%) had PET-MF. Next-generation sequencing (NGS) in 53 patients showed JAK2V617F in 39 patients (74%), CALR mutations in 13 patients (25%) and MPL mutation in 1 patient (2%). On day 1, the mean white blood cell (WBC) count, hemoglobin concentration, platelet count, and lactate dehydrogenase (LDH) level were 9.4 x 109/L (range: 3.3-33.95), 12.5 g/dL (range: 7.1-15.9), 520 x 109/L (range: 113-1038) and 359 IU/L (range: 136-1146) respectively. The median duration of follow-up was 24 (2-28) weeks. Forty-six and 30 patients completed 12 and 24 weeks of treatment respectively. At 12 and 24 weeks the CHCR rate was 74% and 67%, respectively. At 24 weeks, 36 (92%) of 39 JAK2V617F mutated patients had stable or improved JAK2V617F allele burden by droplet digital polymerase reaction (ddPCR). Three patients (8%) had >50% reduction in the JAK2V617F allele burden with one patient achieving undetectable JAK2V617F from week 16 onwards. Disease progression or blastic transformation were not observed during the study. The most common AEs were malaise (N=22, 39%; Grade 1-2, N=21; Grade 3-4, N=1), anemia (N=12, 21%; Grade 1-2, N=8; Grade 3-4, N=4), muscle pain (N=11, 20%; Grade 1-2, N=11; Grade 3-4, N=0) and hair loss (N=10, 18%; Grade 1-2, N=10; Grade 3-4; N=0) . There were no treatment discontinuations, safety signals or deaths related to treatment. In summary, P1101 was well-tolerated, effective in cytoreduction and induced molecular responses. The recruitment of patients is ongoing.

Dietary selenium levels modulates antioxidant, cytokine and immune response and selenoproteins mRNA expression in rats under heat stress condition

BackgroundHigher environmental temperature is a major abiotic stress factor for animals and human beings. The selenium (Se) is an important trace mineral having diverse health promoting effects under stress conditions. However, studies on dietary requirement of selenium under prolonged heat stress condition are lacking. Present study discern the effect of higher dietary Se levels on antioxidant, cytokine, haemato-biochemical profile, and immune response, and the selenoproteins mRNA expression in rats under prolonged heat stress (HS) condition. MethodsWeaned Wistar rats (4 wk age; 67.6 ± 1.53 g BW; n = 72) housed under thermoneutral (TN) or HS conditions and fed with purified diets containing three graded Se levels were divided in six experimental groups. The groups were 1) TN control with 138 ppb Se (TN_CON), 2) HS control with 138 ppb Se (HS_CON), 3) TN with higher Se @ 291 ppb (TN_Se1), 4) HS with higher Se @ 291 ppb (HS_Se1) 5) TN with higher Se @ 460 ppb (TN_Se2), 6) HS with higher Se @ 460 ppb (HS_Se2). Rats in all the six groups were maintained in TN environmental conditions (57.3 ± 0.22 temperature humidity index; THI) for initial 28 days period. Subsequently, rats of HS groups were exposed to 77.0 ± 0.11 THI for 6 h/d in a psychrometric chamber for last fourteen days. ResultsHigher dietary Se (291 and 460 ppb) significantly improved the blood hemoglobin concentration and reduced serum alanine aminotransferase activity of rats under HS conditions. The serum triiodothyronine and insulin levels were significantly higher in high dietary Se groups irrespective of the environmental conditions. Similarly, the serum reduced glutathione levels, and catalase and superoxide dismutase enzyme activity were increased and malondialdehyde levels were reduced in high dietary Se groups irrespective of stress conditions. The glutathione peroxidase (GPx) activity was significantly higher in 460 ppb dietary Se groups as compared to other groups. The serum pro-inflammatory cytokine interleukin (IL)− 1 was declined, whereas the anti-inflammatory cytokine IL-10 level was increased in high dietary Se fed rats under both HS and TN conditions with 460 ppb dietary Se groups showing pronounced effects. Further, there was heat stress- and dietary Se level dependent- up regulation in hepatic GPx and iodothyronine deiodinase-II mRNA expression and similar pattern was noticed in hepatic thioredoxin reductase mRNA expression. The selenoprotein-P mRNA expression was up regulated in 460 ppb Se fed HS group as compared to CON and Se1_C groups. High dietary Se improved the humoral immune response 7d after antigen inoculation under HS conditions whereas cell-mediated immune response was augmented in rats fed higher Se under TN condition. ConclusionIt is concluded that under prolonged heat stress conditions the dietary requirement of Se may be increased to 460 ppb for improving the antioxidant status and humoral immune response, cytokine levels, modulating the thyroid and insulin hormone, and the selenoproteins mRNA expression of rats.

Effect of Smoking on the Red Blood Cell Count, Hemoglobin Concentration, Hematocrit and Red Cell Indices in Adult Male Smokers

Background- Cigarette smoking is one of the common addictions. The effects of cigarette smoking on health are serious and, in many cases, deadly.&#x0D; Objectives: The present study was carried to observe the effect of smoking on Red Blood Cells (RBC) count, Hemoglobin (Hb) concentration, Hematocrit (HCT) and Red cell indices in adult male smokers and compare it with non-smokers to create awareness.&#x0D; Materials and Methods: In this study 50 adult male smokers and 50 adult male non-smokers aged from 20-45 years were taken. The smokers were regularly smoking for at least one year. Fresh peripheral blood sample from subjects were collected and analyzed for Red blood cell count, Hemoglobin concentration, Hematocrit and Red cell indices by using Mythmic-22 automated 5-part Hematology Analyzer.&#x0D; Result: In this study the values of RBCs count, Hb concentration and Hematocrit showed significant increase (p&lt;0.05) in smokers as compared to non-smokers. The values of Red cell indices (MCV, MCH, MCHC) were statistically not significant between two groups.&#x0D; Conclusion: This study concluded that smoking causes persistent state of hypoxia in the body due to smoke contents which cause increase in RBCs count, Hb concentration and Hematocrit.&#x0D; EMCJ. January 2022; 7(1): 1-5

High frequencies of vitamin B12 and folic acid deficiencies and hyperhomocysteinemia in Taiwanese male patients with oral submucous fibrosis

Background/purpose: Oral submucous fibrosis (OSF) is a betel quid chewing-caused oral mucosal disease with progressive collagen deposition. This study evaluated whether Taiwanese male OSF patients had high frequencies of vitamin B12 and folic acid deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity. Materials and methodsThe blood hemoglobin (Hb), serum iron, vitamin B12, folic acid, homocysteine, and GPCA concentrations in 62 male OSF patients were measured and compared with the corresponding data in 124 age-matched male healthy control subjects. ResultsWe found that 5 (8.1%), 12 (19.4%), 32 (51.6%), 31 (50.0%), 22 (35.5%), and 6 (9.7%) of the 62 male OSF patients had Hb (<13 g/dL), iron (≤70 μg/dL), vitamin B12 (≤450 pg/mL), and folic acid (≤6 ng/mL) deficiencies, hyperhomocysteinemia (>12 μM), and serum GPCA positivity, respectively. Furthermore, OSF patients had significantly higher frequencies of Hb (P = 0.006), vitamin B12 (P < 0.001), and folic acid (P < 0.001) deficiencies, hyperhomocysteinemia (P < 0.001), and serum GPCA positivity (P = 0.030) than 124 healthy control subjects. Of the 22 OSF patients with hyperhomocysteinemia, 4 had vitamin B12 deficiency only, 7 had folic acid deficiency only, and 11 had both vitamin B12 and folic acid deficiencies. ConclusionWe conclude that Taiwanese male OSF patients have high frequencies of vitamin B12 and folic acid deficiencies, hyperhomocysteinemia, and serum GPCA positivity. The hyperhomocysteinemia in our OSF patients is predominantly due to deficiencies of either vitamin B12 or folic acid or both.

BIOCHEMICAL ENERGY AND HEMATOLOGICAL PROFILE OF SHEEP SUPPLEMENTED WITH SOY MOLASSES

Soy molasses is a by-product of the soy industry, with low commercial cost and rich in carbohydrates and proteins. Despite the great variety of by-products and being potentially beneÞ cial, it is not fully understood how the interaction between the nutrients in this product and the physiological changes in sheep occurs. Thus, the objective was to evaluate the effects of supplementation levels with soy molasses on the energy metabolites and hematological parameters of sheep. The treatments consisted of 0, 3, 6, 9, and 12% of inclusion of soy molasses in dry matter basis for a basal diet of corn silage, which met the nutritional needs of the sheep in maintenance, distributed in a 5 × 5 Latin square design. The energy metabolites evaluated were: cholesterol, triglycerides, very-low-density lipoproteins (VLDL), and fructosamine. The hematological parameters were: red blood cells, hemoglobin, hematocrits, and platelets, in addition to leukocytes, rods, neutrophils, eosinophils, monocytes, and lymphocytes. Supplementation with soy molasses did not signiÞ cantly alter (P&gt; 0.05) the serum concentrations of cholesterol, triglycerides, VLDL, and fructosamine. There was no signiÞ cant difference in the blood cell, hemoglobin, hematocrit, and platelet concentrations in sheep receiving soy molasses supplementation (P&gt; 0.05), which may indicate that the level of inclusion was sufÞ cient to meet the nutritional needs of the animals, without damage. Leukocyte, rod, neutrophil, eosinophil, monocyte, and lymphocyte concentrations did not change (P&gt; 0.05) with the inclusion of soy molasses in the sheep’s diet, indicating the absence of intoxication, diseases, and infections. It can be concluded that supplementation with soy molasses does not alter the energy metabolites and hematological parameters of sheep in maintenance.

Preventive effects of selenium against cypermethrin induced haematological toxicity in Sprague-Dawley rats

Purpose: Cypermethrin (class II) belongs to pyrethroid insecticides which have been in wide use to control various pests including moth pests of cotton, fruit and vegetable crops. This study was aimed to investigate the effects of cypermethrin on haematology of female rats and their modulation by co-administration of sodium selenite. Methodology: Four equal groups of female rats were made (n=20). Group 1 received 1 ml corn oil, Group 2 was administered 55 mg/kg/b.w of cypermethrin, Group 3 was provided with 1 ppm of sodium selenite and Group 4 was given both cypermethrin and sodium selenite. Haematological analysis of blood samples was performed employing Sesmex Kx-21 (Japan). Results: Red blood cell (RBC) count, haemoglobin concentration (Hb) and hematocrit (Hct) were significantly reduced (P&lt;0.05) due to cypermethrin treatment which indicates that rats were suffering from anaemia. While, the rest of the hematological parameters i.e. platelet count (PLT), number of lymphocytes, white blood cell (WBC) count, platelet larger cell ratio (P-LCR), RBC distribution width (RDW), mean platelet volume (MPV), platelet distribution width (PDW), lymphocyte percentage (LYM), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) were non-significantly affected. Selenium coadministration with cypermethrin was found to restore the levels of above-mentioned parameters to those found in control group. Conclusion: Hence, it can be concluded from this study that cypermethrin administration can cause hematological alterations in rats. Co-administration of selenium provided protection against cypermethrin toxicity to some extent.

Effect of Anisakis pegreffii (Nematoda: Anisakidae) on Biochemical and Haematological Characteristics of Chub Mackerel (Scomber japonicus Houttuyn, 1782) Caught in the Dardanelles at Çanakkale, Türkiye

Chub mackerel (Scomber japonicus Houttuyn, 1782) were obtained from the commercial fishermen in Çanakkale, Turkey, in July, 2017. We examined a total of 40 fish (20 non-infested and 20 infested) and assessed the biometric indices, haematological parameters and serum biochemical variables. The hepatosomatic index and gonadosomatic index of infested chub mackerel fish were lower than those of non-infested fish. Blood haematocrit ratio and haemoglobin concentration in naturally parasite-infested chub mackerel fish were significantly lower than those in non-infested fish. However, white blood cell counts of the parasite-infested chub mackerel fish were higher than those of healthy ones. Serum total protein, globulin, glucose, cholesterol, triglyceride, urea, chlorine and iron levels in naturally parasite-infested chub mackerel fish were significantly lower than those in non-infested fish. Moreover, serum lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase and alanine aminotransferase activities of the parasite-infested chub mackerel fish were higher than those in healthy ones. Therefore, observed variations in haematological parameters, serum biochemical variables and biometric indices influenced by the parasite, A. pegreffii, may potentially increase sensitivity of the chub mackerel, Scomber japonicus, to diseases and environmental conditions.

A Nano Refractive Index Sensing Structure for Monitoring Hemoglobin Concentration in Human Body.

This paper proposes a nanosensor structure consisting of a metal-insulator-metal (MIM) waveguide with a rectangular root and a double-ring (SRRDR) with a rectangular cavity. In this paper, the cause and internal mechanism of Fano resonance are investigated by the finite element method (FEM), and the transport characteristics are optimized by changing various parameters of the structure. The results show that the structure can achieve double Fano resonance. Due to the destructive disturbance between the wideband mode of the inverted rectangle on the bus waveguide and the narrowband mode of the SRRDR, the output spectrum of the system shows an obvious asymmetric Fano diagram, and the structural parameters of the sensor have a great influence on the Fano resonance. By changing the sensitive parameters, the optimal sensitivity of the refractive index nanosensor is 2280 nm/RIU, and the coefficient of excellence (FOM) is 76.7. In addition, the proposed high-sensitivity nanosensor will be used to detect hemoglobin concentration in blood, which has positive applications for biosensors and has great potential for future nanosensing and optical integration systems.

Исследование изменений состояния эритроцитов и плазмы крови обезьян при воздействии ионизирующего излучения

The effect of ionizing radiation on the blood parameters of monkeys (rhesus monkeys, male species) was studied. Animals were exposed to irradiation with 1/10 of the total dose (50 cSv) for 10 days with an interval of 2 days (Group-1) or 1/2 of the total dose (50 cSv) for 2 days (Group-2). It was found that decrease in the hematocrit was more pronounced on the 36th day during the first irradiation, and on the 64th day after the second one. As a rule, a decrease in hemoglobin content was observed after the first and second irradiations, which could indicate an alteration in the hematopoietic system function. Molecular alterations in blood plasma carotenoids and erythrocyte hemoglobin heme were studied using the Raman spectroscopy. It was proven that after irradiation the microenvironment of carotenoids in plasma lipoprotein complexes in Group-2 became less viscous, than in Group-1. This indicated that on the 36th day processes were triggered in blood plasma of the Group-2 animals that changed the nature of protein-lipid interactions in plasma lipoprotein complexes, where carotenoids were localized. On certain days after irradiation, reliable alterations were registered in the erythrocytes hemoglobin properties in Group-1 and Group-2 compared to each other. It is likely that the second irradiation had a different effect on the erythrocyte hemoglobin: Group-1 irradiation had a more pronounced effect on the erythrocyte hemoglobin than in Group-2. Difference in the erythrocyte hemoglobin affinity to oxygen observed in Group-1 and Group-2 after the first irradiation was not correlating with alterations in the hematocrit or erythrocyte hemoglobin content in blood, but was associated with other processes that affected the erythrocyte hemoglobin conformation. According to the authors of the work, results of studying the carotenoid molecules content or conformation in the blood plasma lipoprotein complexes would allow developing and introducing not only a methodology for diagnosing the state of an organism as a whole, but also the therapy using the natural antioxidants

Cotton gin byproduct: Effects on feed intake, quality, and safety for use in diets of gestating beef cows

The effects of cotton gin byproduct processing and feed safety characteristics are not well defined in beef cattle systems. Our objective was to determine the effects of feeding loose or baled cotton gin byproduct on animal performance and safety of use in beef cattle diets. Twenty-four nonlactating, crossbred cows and heifers (average BW = 613 ± 77 kg) were assigned to 1 of 2 treatments: ad libitum (1) baled cotton gin byproduct or (2) loose cotton gin byproduct. Animal BW and BCS were collected at d 0, 30, and 60 of the study. Jugular blood samples were collected weekly during the trial and again at 30 and 60 d after the study to monitor hematologic responses due to consumption of cotton gin byproduct containing defoliant residue (4.84 and 1.98 mg/kg tribufos for baled and loose, respectively). Cattle consuming loose cotton gin byproduct had greater (P < 0.0001) daily DMI (2.0% of animal BW) than those consuming baled byproduct (1.8% of animal BW). Cattle gained BW and maintained BCS across treatments during the study. White and red blood cell count, hematocrit, and hemoglobin concentrations were within the acceptable range for normal health conditions in beef cattle, and animals displayed no clinical symptoms of altered neurological status due to consuming gin byproduct. Results indicate that cotton gin byproduct in either loose or baled form can be used to maintain nonlactating, bred cows with additional supplementation during a short-term feeding period (60 d).

Analysis of ridges and grooves shape in grating coupler for optimization of integrated optics sensor structures.

The paper presents a theoretical analysis of a sensor structure based on a planar waveguide and grating coupler designed to determine selected physical properties of blood – hemoglobin concentration and oxidation level. In particular analysis were focused on optimization of selected geometrical properties of grating coupler (shape of ridges and grooves) to obtain maximum efficiency of uncoupling of light from the sensor structure. The analysis were carried out for three type of ridges and grooves shape in grating coupler: rectangular, triangular and sinusoidal. Full Text: PDF ReferencesI. . Singh, A.Weston, A. Kundur, G. Dobie, Haematology Case Studies with Blood Cell Morphology and Pathophysiology; Elsevier: Amsterdam, The Netherlands, (2017). DirectLink P. Jarolim, M. Lahav, SC. Liu, J. Palek, "Effect of hemoglobin oxidation products on the stability of red cell membrane skeletons and the associations of skeletal proteins: correlation with a release of hemin", Blood 76, 10 (1990). CrossRef E. Beutler, J. Waalen, "The definition of anemia: what is the lower limit of normal of the blood hemoglobin concentration?", Blood 107, 5 (2006). CrossRef M. Kiroriwal, P. Singal M. Sharma, A. Singal, "Hemoglobin sensor based on external gold-coated photonic crystal fiber", Optics &amp; Laser Technology 149, 107817 (2022). CrossRef A. A. Boiarski, J. R. Busch, B. S. Bhullar, R. W. Ridgway, V. E. Wood, "Integrated optic sensor with macro-flow cell", Proc. SPIE Integrated Optics and Microstructures 1793 (1993). CrossRef L. Cheng, S. Mao, Z. Li, Y. Han and H. Y. Fu, "Grating Couplers on Silicon Photonics: Design Principles, Emerging Trends and Practical Issues", Micromachines 11, 666 (2020). CrossRef P. Struk, "Design of an Integrated Optics Sensor Structure Based on Diamond Waveguide for Hemoglobin Property Detection", Materials 12, 175 (2019). CrossRef P. Struk, "Numerical analysis of integrated photonics structures for hemoglobin sensor application", Phot. Lett. Poland 12, 2 (2020). CrossRef P.V. Lambeck, "Integrated optical sensors for the chemical domain", Meas. Sci. Technol. 17, (2006). CrossRef W. Lukosz, "Integrated optical chemical and direct biochemical sensors", Sens. Actuators B Chem 29 (1995). CrossRef P. Struk, T. Pustelny, K. Gołaszewska,E. Kaminska, M.A. Borysiewicz, M. Ekielski, A. Piotrowska, "Hybrid photonics structures with grating and prism couplers based on ZnO waveguides", Opto-Electron. Rev. 21, (2013). CrossRef P. Struk, "Design of an integrated optics sensor structure for hemoglobin property detection", Proc. SPIE 11204, (2019). CrossRef OptiFDTD Technical Background and Tutorials - Finite Difference Time Domain Photonics Simulation Software, Optiwave Systems Inc. (2008). DirectLink K. Yee, "Cutoff Frequencies of Eccentric Waveguides", IEEE Transactions 14, 3 (1966). CrossRef