Hemibiotrophic Fungus Research Articles

Organic acids and glucose prime late-stage fungal biotrophy in maize.

Many plant-associated fungi are obligate biotrophs that depend on living hosts to proliferate. However, little is known about the molecular basis of the biotrophic lifestyle, despite the impact of fungi on the environment and food security. In this work, we show that combinations of organic acids and glucose trigger phenotypes that are associated with the late stage of biotrophy for the maize pathogen Ustilago maydis. These phenotypes include the expression of a set of effectors normally observed only during biotrophic development, as well as the formation of melanin associated with sporulation in plant tumors. U. maydis and other hemibiotrophic fungi also respond to a combination of carbon sources with enhanced proliferation. Thus, the response to combinations of nutrients from the host may be a conserved feature of fungal biotrophy.

Deep learning uncovers distinct behavior of rice network to pathogens response

SummaryRice, apart from abiotic stress, is prone to attack from multiple pathogens. Predominantly, the two rice pathogens, bacterial Xanthomonas oryzae (Xoo) and hemibiotrophic fungus, Magnaporthe oryzae, are extensively well explored for more than the last decade. However, because of lack of holistic studies, we design a deep learning-based rice network model (DLNet) that has explored the quantitative differences resulting in the distinct rice network architecture. Validation studies on rice in response to biotic stresses show that DLNet outperforms other machine learning methods. The current finding indicates the compactness of the rice PTI network and the rise of independent modules in the rice ETI network, resulting in similar patterns of the plant immune response. The results also show more independent network modules and minimum structural disorderness in rice-M. oryzae as compared to the rice-Xoo model revealing the different adaptation strategies of the rice plant to evade pathogen effectors.

Bioinspired Rhamnolipid Protects Wheat Against Zymoseptoria tritici Through Mainly Direct Antifungal Activity and Without Major Impact on Leaf Physiology.

Rhamnolipids (RLs), glycolipids biosynthesized by the Pseudomonas and Burkholderia genera, are known to display various activities against a wide range of pathogens. Most previous studies on RLs focused on their direct antimicrobial activity, while only a few reports described the mechanisms by which RLs induce resistance against phytopathogens and the related fitness cost on plant physiology. Here, we combined transcriptomic and metabolomic approaches to unravel the mechanisms underlying RL-induced resistance in wheat against the hemibiotrophic fungus Zymoseptoria tritici, a major pathogen of this crop. Investigations were carried out by treating wheat plants with a bioinspired synthetic mono-RL with a 12-carbon fatty acid tail, dodecanoyl α/β-L-rhamnopyranoside (Rh-Est-C12), under both infectious and non-infectious conditions to examine its potential wheat defense-eliciting and priming bioactivities. Whereas, Rh-Est-C12 conferred to wheat a significant protection against Z. tritici (41% disease severity reduction), only a slight effect of this RL on wheat leaf gene expression and metabolite accumulation was observed. A subset of 24 differentially expressed genes (DEGs) and 11 differentially accumulated metabolites (DAMs) was scored in elicitation modalities 2, 5, and 15 days post-treatment (dpt), and 25 DEGs and 17 DAMs were recorded in priming modalities 5 and 15 dpt. Most changes were down-regulations, and only a few DEGs and DAMs associated with resistance to pathogens were identified. Nevertheless, a transient early regulation in gene expression was highlighted at 2 dpt (e.g., genes involved in signaling, transcription, translation, cell-wall structure, and function), suggesting a perception of the RL by the plant upon treatment. Further in vitro and in planta bioassays showed that Rh-Est-C12 displays a significant direct antimicrobial activity toward Z. tritici. Taken together, our results suggest that Rh-Est-C12 confers protection to wheat against Z. tritici through direct antifungal activity and, to a lesser extent, by induction of plant defenses without causing major alterations in plant metabolism. This study provides new insights into the modes of action of RLs on the wheat-Z. tritici pathosystem and highlights the potential interest in Rh-Est-C12, a low-fitness cost molecule, to control this pathogen.

Ethylene activates poplar defense against Dothiorella gregaria Sacc by regulating reactive oxygen species accumulation.

Populus canker is a widespread disease that seriously threatens the survival of trees. Phytohormones are considered as effective chemical molecules improving plant resistance to various diseases. Ethylene is an important phytohormone that is extensively involved in the regulation of plant growth, development, and stress responses, but how ethylene and ethylene signaling regulates defense responses in woody plants is still unclear. Here, we showed that ethylene positively regulates the responses of poplar to canker caused by the hemibiotrophic fungus Dothiorella gregaria. Treatment of Populus tomentosa with 1-aminocyclopropane-1-carboxylic acid (ACC, the biosynthetic precursor of ethylene) significantly enhanced disease resistance, accompanied by the induction of pathogen-related protein (PR) gene expression and H2 O2 accumulation. Blocking ethylene biosynthesis using aminoethoxyvinyl glycine (AVG, a specific inhibitor of ethylene biosynthesis) repressed the disease resistance. Overexpression of the ethylene biosynthesis gene PtoACO7 in Populus tomentosa promoted defense responses and disease resistance. Furthermore, we demonstrated that the ethylene-induced defense response is independent of the salicylic acid pathway, but needs ROS signaling. ACC or PtoACO7 overexpression induced expressions of PtoRbohD/RbohF, which encode NADPH oxidases, and elevated H2 O2 levels in poplar. Inhibition of the NADPH oxidase compromised ethylene-induced disease resistance and PR gene expressions, while H2 O2 application could completely rescue the AVG-caused disease hypersensitivity. Therefore, the involvement of ethylene in disease resistance is done by activation of PR gene expressions and ROS production. Our results also showed that modifying ethylene biosynthesis or its signaling pathway has a great potential for improving disease resistance in woody plants.

Transcriptome Analysis and Functional Validation Identify a Putative bZIP Transcription Factor, Fpkapc, that Regulates Development, Stress Responses, and Virulence in Fusarium pseudograminearum.

Fusarium pseudograminearum is a soilborne, hemibiotrophic phytopathogenic fungus that causes Fusarium crown rot and Fusarium head blight in wheat. The basic leucine zipper proteins (bZIPs) are evolutionarily conserved transcription factors that play crucial roles in a range of growth and developmental processes and the responses to biotic and abiotic stresses. However, the roles of bZIP transcription factors remains unknown in F. pseudograminearum. In this study, a bZIP transcription factor Fpkapc was identified to localize to the nucleus in F. pseudograminearum. A mutant strain (Δfpkapc) was constructed to determine the role of Fpkapc in growth and pathogenicity of F. pseudograminearum. Transcriptomic analyses revealed that many genes involved in basic metabolism and oxidation-reduction processes were downregulated, whereas many genes involved in metal iron binding were upregulated in the Δfpkapc strain, compared with the wild type (WT). Correspondingly, the mutant had severe growth defects and displayed abnormal hyphal tips. Conidiation in the Fpkapc mutant was reduced, with more conidia in smaller size and fewer septa than in the WT. Also, relative to WT, the Δfpkapc strain showed greater tolerance to ion stress, but decreased tolerance to H2O2. The mutant caused smaller disease lesions on wheat and barley plants, but significantly increased TRI gene expression, compared with the WT. In summary, Fpkapc plays multiple roles in governing growth, development, stress responses, and virulence in F. pseudograminearum.

Verticillium dahliae Secretes Small RNA to Target Host MIR157d and Retard Plant Floral Transition During Infection.

Bidirectional trans-kingdom RNA silencing [or RNA interference (RNAi)] plays a key role in plant-pathogen interactions. It has been shown that plant hosts export specific endogenous miRNAs into pathogens to inhibit their virulence, whereas pathogens deliver small RNAs (sRNAs) into plant cells to disturb host immunity. Here, we report a trans-kingdom fungal sRNA retarding host plant floral transition by targeting a miRNA precursor. From Arabidopsis plants infected with Verticillium dahliae, a soil-borne hemibiotrophic pathogenic fungus that causes wilt diseases in a wide range of plant hosts, we obtained a number of possible trans-kingdom V. dahliae sRNAs (VdsRNAs) by sequencing AGO1-immunoprecipitated sRNAs. Among these, a 24-nt VdsRNA derived from V. dahliae rRNA, VdrsR-1, was shown to be an actual trans-kingdom VdsRNA that targets the miR157d precursor MIR157d, resulting in increased rather than reduced miR157d accumulation in V. dahliae-infected plants. Consistent with the miR157 family in the regulation of vegetative and floral transitions by targeting SPL genes in several plant species, we detected two SPL genes, SPL13A/B, that were notably reduced in V. dahliae-infected and VdrsR-1-expressing plants compared with control plants. Furthermore, V. dahliae-infected and VdrsR-1-expressing plants also displayed delayed vegetative phase change and floral transition compared to control plants. Taken together, we disclosed a novel mode of action for a trans-kingdom fungal sRNA, VdrsR-1, which was secreted into host cells to modulate plant floral transition by employing the miR157d/SPL13A/B regulatory module, leading to prolonged host vegetative growth that would undoubtedly benefit fungal propagation.

Overexpression of the Potato Monosaccharide Transporter StSWEET7a Promotes Root Colonization by Symbiotic and Pathogenic Fungi by Increasing Root Sink Strength.

Root colonization by filamentous fungi modifies sugar partitioning in plants by increasing the sink strength. As a result, a transcriptional reprogramming of sugar transporters takes place. Here we have further advanced in the characterization of the potato SWEET sugar transporters and their regulation in response to the colonization by symbiotic and pathogenic fungi. We previously showed that root colonization by the AM fungus Rhizophagus irregularis induces a major transcriptional reprogramming of the 35 potato SWEETs, with 12 genes induced and 10 repressed. In contrast, here we show that during the early colonization phase, the necrotrophic fungus Fusarium solani only induces one SWEET transporter, StSWEET7a, while represses most of the others (25). StSWEET7a was also induced during root colonization by the hemi-biotrophic fungus Fusarium oxysporum f. sp. tuberosi. StSWEET7a which belongs to the clade II of SWEET transporters localized to the plasma membrane and transports glucose, fructose and mannose. Overexpression of StSWEET7a in potato roots increased the strength of this sink as evidenced by an increase in the expression of the cell wall-bound invertase. Concomitantly, plants expressing StSWEET7a were faster colonized by R. irregularis and by F. oxysporum f. sp. tuberosi. The increase in sink strength induced by ectopic expression of StSWEET7a in roots could be abolished by shoot excision which reverted also the increased colonization levels by the symbiotic fungus. Altogether, these results suggest that AM fungi and Fusarium spp. might induce StSWEET7a to increase the sink strength and thus this gene might represent a common susceptibility target for root colonizing fungi.

Transgenic tomato expressing an oxalate decarboxylase gene from Flammulina sp. shows increased survival to Moniliophthora perniciosa

Witches’ broom disease which affects cacao tree (Theobroma cacao L.) is caused by the hemibiotrophic fungus Moniliophthora perniciosa. Studies of the cacao-M. perniciosa interaction showed that the pathogenicity of M. perniciosa is associated to the presence of oxalic acid (OA). In some organisms, OA can be degraded into formate and CO2 by the oxalate decarboxylase (OXDC) enzyme. Here, we used the oxdc gene from Flammulina velutipes to generate transgenic tomato plants as a potential strategy to control M. perniciosa infection. Three transgenic T1 lines expressing the oxdc gene were obtained and two of them (P4 and P16) presented a significant delay in symptom development and higher survival percentage when inoculated with M. perniciosa in comparison to the non-transformed wild-type (WT) plants. Moreover, the P16 line test showed a decreased production of H2O2 in the presence of OA in comparison to the WT, as evidenced by the 3,3′-diaminobenzidine test. These results indicate that the P16 transgenic line is able to break down OA leading to normal plant development with few symptoms even when infected by M. perniciosa. Phenotypic and histological analysis showed differences between WT and transgenic plants. The amount of fungus hyphae was lower with thinner thickness in the transgenic plants. This study evidences of the reduction of witches’ broom disease progression by the presence of the oxdc gene leading to the plant survival.

Transcriptional Network in Colletotrichum gloeosporioides Mutants Lacking Msb2 or Msb2 and Sho1.

Colletotrichum gloeosporioides is a hemibiotrophic ascomycetous fungus that causes anthracnose in many plants worldwide. During infections, C. gloeosporioides produces an appressorium in response to various plant surface signals. However, the mechanism mediating host surface signal recognition remains unclear. In this study, C. gloeosporioides ΔCgMsb2 and ΔCgMsb2Sho1 mutants lacking hypothetical sensors of plant surface signals were examined. The mutations in ΔCgMsb2 and ΔCgMsb2Sho1 adversely affected conidial size and sporulation, while also inhibiting growth. Significant transcriptional changes were detected for nearly 19% and 26% of the genes in ΔCgMsb2 and ΔCgMsb2Sho1, respectively. The lack of these plasma membrane receptors altered the expression of specific genes, especially those encoding hydrolases, ABC transporters, and mitogen-activated protein kinases (MAPKs). The encoded MAPKs participate in the signal transduction of ERK and JNK signaling pathways, activate downstream signals, and contribute to metabolic regulation. Our data demonstrate that the C. gloeosporioides membrane proteins Msb2 and Sho1 affect gene regulation, thereby influencing conidial growth, metabolism, and development. These findings provide new insights into the regulation of C. gloeosporioides’s development and infection of plant hosts.

Decrease of the negative impact on environment of protectants of seed which contain tebuconazole and prochloraz for sustainable agricultural development

Fungi that parasitize plants are capable of producing plant toxins - phytotoxins. One of the producers of phytotoxins is the hemibiotrophic fungus Pyrenophora sp. Phytotoxins inhibit cell division, suppress or enhance respiration, disrupt synthesis, transport, membrane functions, inhibit enzymes, exhibit hormonal activity - they produce phytohormone abscisic acid. Many fungicides, in addition to the protective effect, have a toxic effect both independently and indirectly, through the effect on the production of toxins by the fungus. We propose to call the first property toxicity, the second - toxigenicity. The aim of this work was to clarify the inhibitory effect on plant growth and the toxigenic effect on phytopathogenic fungi tebuconazole and prochloraz. Tebuconazole and prochloraz in the doses used had a fungistatic effect. The synergistic effect is revealed. Since the effect of tebuconazole was greater than that of prochloraz, it would be prudent to reduce the dose in the dressing agent. The optimal ratios were 1: 5; 3: 1; 3: 3. At high doses of tebuconazole, the synergistic effect with prochloraz was not manifested. The toxicity of the prochloraz solution for seedlings was higher than the toxicity of tebuconazole; both those a.i. did not significantly affect the shoot/root ratio, did not change the hormonal balance. The most favorable ratios were tebuconazole: prochloraz 1: 2, 3: 2. The toxicity of prochloraz for the mycelium of Pyrenophora sp., assessed by the biotest, was higher than the toxicity of tebuconazole. Inhibition of root growth was noted. The least toxigenic ratios were tebuconazole: prochloraz 3: 2 and 5: 4. According to the complex of features, the ratio tebuconazole: prochloraz 3: 2 is the most effective, least toxic for plants and toxigenic for the phytopathogenic fungus Pyrenophora sp.

Screening of common bean (Phaseolus vulgaris L.) germplasm against Colletotrichum lindemuthianum inciting bean anthracnose

Common bean is the most cultivated grain legume consumed globally. It is enriched in proteins, vitamins, minerals and fiber. Anthracnose disease incited by hemibiotrophic fungi Colletotrichum lindemuthianum is the most destructive disease of common beans. The present study was carried out to evaluate 87 common bean genotypes collected from different locations against C. lindemuthianum. Screening of genotypes for anthracnose resistance aids in the identification of 22 highly resistant genotypes namely PL 1, EC-400397, Hur 137, IC-199277, IC-258273, S 2, EC-400442, KB 4, Utkarsh, Hur 15, IC-260299, PDR 14, VL 125, Amber, Arun, EC-398591, EC-121013, S 6, BR 31, IC- 328372, KB 12 and KB 6. These genotypes can act as potential donors of the resistant genes in markerassisted selection (MAS) programmes for transferring the anthracnose resistance gene(s) into the susceptible genotypes.

Elucidation of the biochemical and molecular basis of the differential disease expression in two cultivars of chili (Capsicum annuum) in response to Colletotrichum capsici infection.

Chili plants are affected by the hemibiotrophic ascomycota fungus Colletotrichum capsici causing Anthracnose. Infection results in yield and marketability loss due to a decrease in the quality of fruits. The study of morphological symptom development in two cultivars, Bullet, and Beldanga, showed very different disease expression pattern. To understand the reasons behind such differential response, we investigated, in a time-dependent manner, biochemical activities of important defense enzymes, PR proteins, like peroxidase, polyphenol-oxidase, phenylalanine ammonia lyase, β-glucanase, chitinase, catalase, as well as phenols, flavonoids, chlorophyll and the key signaling molecule nitric oxide in their leaves. We further performed real-time nitric oxide (NO) detection studies. The results showed striking differences in the activity profile of these defense molecules through the course of the study. We monitored the gene expression levels of 12 important defense-related genes under in vivo condition. The transcription levels were mostly increased in the tolerant cultivar till 7 days post-infection (DPI), while downregulation of some of the genes were observed in the susceptible one. These data indicated that disease manifestation is a simulated response of these defense molecules which can nullify the effect of the pathogen and its products, when resistance occurs. Alternatively, the pathogen suppresses the host defense when the disease develops.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11738-021-03334-x.

Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development

Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches’ broom disease in cacao (Theobroma cacao L.). The biotrophic fungal phase initiates the disease and is characterized by a monokaryotic mycelium, while the necrotrophic phase is characterized by a dikaryotic mycelium and leads to necrosis of infected tissues. A study of the necrotrophic phase was conducted on bran-based solid medium, which is the only medium that enables basidiocarp and basidiospore production. Six different fungal developmental phases were observed according to the mycelium colour or the organ produced: white, yellow, pink, dark pink, primordium and basidiocarp. In this study, we identified notable proteins in each phase, particularly those accumulated prior to basidiocarp formation. Proteins were analysed by proteomics; 2-D gels showed 300–550 spots. Statistically differentially accumulated spots were sequenced by mass spectrometry and 259 proteins were identified and categorized into nine functional classes. Proteins related to energy metabolism, protein folding and morphogenesis that were potentially involved in primordium and basidiocarp formation were identified; these proteins may represent useful candidates for further analysis related to the spread and pathogenesis of this fungus. To the best of our knowledge, this report describes the first proteomic analysis of the developmental phases of Moniliophthora perniciosa.

Identification of a bio-signature for barley resistance against Pyrenophora teres infection based on physiological, molecular and sensor-based phenotyping

Necrotic and chlorotic symptoms induced during Pyrenophora teres infection in barley leaves indicate a compatible interaction that allows the hemi-biotrophic fungus Pyrenophora teres to colonise the host. However, it is unexplored how this fungus affects the physiological responses of resistant and susceptible cultivars during infection. To assess the degree of resistance in four different cultivars, we quantified visible symptoms and fungal DNA and performed expression analyses of genes involved in plant defence and ROS scavenging. To obtain insight into the interaction between fungus and host, we determined the activity of 19 key enzymes of carbohydrate and antioxidant metabolism. The pathogen impact was also phenotyped non-invasively by sensor-based multireflectance and –fluorescence imaging.Symptoms, regulation of stress-related genes and pathogen DNA content distinguished the cultivar Guld as being resistant. Severity of net blotch symptoms was also strongly correlated with the dynamics of enzyme activities already within the first day of infection. In contrast to the resistant cultivar, the three susceptible cultivars showed a higher reflectance over seven spectral bands and higher fluorescence intensities at specific excitation wavelengths. The combination of semi high-throughput physiological and molecular analyses with non-invasive phenotyping enabled the identification of bio-signatures that discriminates the resistant from susceptible cultivars.

Genome Sequence Resource for Colletotrichum viniferum, the Cause of Grapevine Ripe Rot in China.

Grape ripe rot is an important disease that has seriously damaged the yield and quality of grape worldwide. The disease is caused by Colletotrichum viniferum, a hemibiotrophic fungus that belongs to the Glomerellaceae family of Sordariomycetes class. Here, we present the genome of C. viniferum CvYL2a from grape, based on Illumina HiSeq 2500 and PacBio RS II. The high-quality genome consists of 70 contigs with a 73.41 Mb genome size and encodes 14,668 protein-coding genes. These genes were annotated using Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, EuKaryotic Orthologous Groups, Nonredundant Protein, and Swiss-Prot databases. In addition, we identified a series of genes involved in pathogenicity, including 909 carbohydrate-active enzymes, 67 secondary metabolite gene clusters, and 307 cytochrome P450 enzymes. This genome sequence provides a valuable reference for research on grape-C. viniferum interactions, the pathogenesis of C. viniferum, and comparative genome analyses.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Assessment of the Spatial Distribution and Risk Associated with Fruit Rot Disease in Areca catechu L.

Phytophthora meadii (McRae) is a hemibiotrophic oomycete fungus that infects tender nuts, growing buds, and crown regions, resulting in fruit, bud, and crown rot diseases in arecanut (Areca catechu L.), respectively. Among them, fruit rot disease (FRD) causes serious economic losses that are borne by the growers, making it the greatest yield-limiting factor in arecanut crops. FRD has been known to occur in traditional growing areas since 1910, particularly in Malnad and coastal tracts of Karnataka. Systemic surveys were conducted on the disease several decades ago. The design of appropriate management approaches to curtail the impacts of the disease requires information on the spatial distribution of the risks posed by the disease. In this study, we used exploratory survey data to determine areas that are most at risk. Point pattern (spatial autocorrelation and Ripley’s K function) analyses confirmed the existence of moderate clustering across sampling points and optimized hotspots of FRD were determined. Geospatial techniques such as inverse distance weighting (IDW), ordinary kriging (OK), and indicator kriging (IK) were performed to predict the percent severity rates at unsampled sites. IDW and OK generated identical maps, whereby the FRD severity rates were higher in areas adjacent to the Western Ghats and the seashore. Additionally, IK was used to identify both disease-prone and disease-free areas in Karnataka. After fitting the semivariograms with different models, the exponential model showed the best fit with the semivariogram. Using this model information, OK and IK maps were generated. The identified FRD risk areas in our study, which showed higher disease probability rates (>20%) exceeding the threshold level, need to be monitored with the utmost care to contain and reduce the further spread of the disease in Karnataka.

Fusarium oxysporum f. sp. niveum Molecular Diagnostics Past, Present and Future.

Watermelon is an important commercial crop in the Southeastern United States and around the world. However, production is significantly limited by biotic factors including fusarium wilt caused by the hemibiotrophic fungus Fusarium oxysporum forma specialis niveum (Fon). Unfortunately, this disease has increased significantly in its presence over the last several decades as races have emerged which can overcome the available commercial resistance. Management strategies include rotation, improved crop resistance, and chemical control, but early and accurate diagnostics are required for appropriate management. Accurate diagnostics require molecular and genomic strategies due to the near identical genomic sequences of the various races. Bioassays exist for evaluating both the pathogenicity and virulence of an isolate but are limited by the time and resources required. Molecular strategies are still imperfect but greatly reduce the time to complete the diagnosis. This article presents the current state of the research surrounding races, both how races have been detected and diagnosed in the past and future prospects for improving the system of differentiation. Additionally, the available Fon genomes were analyzed using a strategy previously described in separate formae speciales avirulence gene association studies in Fusarium oxysporum races.

High-Throughput Sequencing Reveals the Regulatory Networks of Transcriptome and Small RNAs During the Defense Against Marssonina brunnea in Poplar

MicroRNAs are implicated in the adjustment of gene expression in plant response to biotic stresses. However, the regulatory networks of transcriptome and miRNAs are still poorly understood. In the present study, we ascertained the induction of genes for small RNA biosynthesis in poplar defense to a hemibiotrophic fungus Marssonina brunnea and afterward investigated the molecular regulatory networks by performing comprehensive sequencing analysis of mRNAs and small RNAs in M. brunnea-inoculated leaves. Differentially expressed genes in M. brunnea-infected poplar are mainly involved in secondary metabolisms, phytohormone pathways, the recognition of pathogens, and MAPK pathway in the plant, with real-time quantitative PCR (qPCR) validating the mRNA-seq results. Furthermore, differentially expressed miRNAs, such as MIR167_1-6, MIR167_1-12, MIR171_2-3, MIR395-13, MIR396-3, MIR396-16, MIR398-8, and MIR477-6, were identified. Through psRobot and TargetFinder programs, MIR167-1-6, MIR395-13, MIR396-3, MIR396-16, and MIR398-8 were annotated to modulate the expression of genes implicated in transportation, signaling, and biological responses of phytohormones and activation of antioxidants for plant immunity. Besides, validated differentially expressed genes involved in lignin generation, which were phenylalanine ammonia-lyase, ferulate-5-hydroxylase, cinnamyl alcohol dehydrogenase, and peroxidase 11, were selected as targets for the identification of novel miRNAs. Correspondingly, novel miRNAs, such as Novel MIR8567, Novel MIR3228, Novel MIR5913, and Novel MIR6493, were identified using the Mireap online program, which functions in the transcriptional regulation of lignin biosynthesis for poplar anti-fungal response. The present study underlines the roles of miRNAs in the regulation of transcriptome in the anti-fungal response of poplar and provides a new idea for molecular breeding of woody plants.

A double‐edged sword: reactive oxygen species (ROS) during the rice blast fungus and host interaction

Magnaporthe oryzae is a hemibiotrophic fungus that also needs host nutrients for propagation during infection. During its interaction with rice, reactive oxygen species (ROS) mediate important signaling reactions impacting both the pathogen and the host. In M. oryzae, the accumulation of ROS is important for the formation and maturation of the infectious structure appressorium. On the other hand, upon M. oryzae infection, rice generates further ROS to restrict invasive hyphae (IH) spreading. Despite ROS receptors remaining to be identified, M. oryzae recruits several strategies to respond and suppress ROS accumulation through the secretion of various effector molecules. These findings suggest that the balance between the generation and scavenging of ROS is sophisticatedly controlled during M. oryzae-rice interaction. In this review, we discuss advances to understand the regulation mechanisms for the generation, accumulation, and transduction of ROS.

The Algal Polysaccharide Ulvan Induces Resistance in Wheat Against Zymoseptoria tritici Without Major Alteration of Leaf Metabolome

This study aimed to examine the ability of ulvan, a water-soluble polysaccharide from the green seaweed Ulva fasciata, to provide protection and induce resistance in wheat against the hemibiotrophic fungus Zymoseptoria tritici. Matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis indicated that ulvan is mainly composed of unsaturated monosaccharides (rhamnose, rhamnose-3-sulfate, and xylose) and numerous uronic acid residues. In the greenhouse, foliar application of ulvan at 10 mg.ml–1 2 days before fungal inoculation reduced disease severity and pycnidium density by 45 and 50%, respectively. Ulvan did not exhibit any direct antifungal activity toward Z. tritici, neither in vitro nor in planta. However, ulvan treatment significantly reduced substomatal colonization and pycnidium formation within the mesophyll of treated leaves. Molecular assays revealed that ulvan spraying elicits, but does not prime, the expression of genes involved in several wheat defense pathways, including pathogenesis-related proteins (β-1,3-endoglucanase and chitinase), reactive oxygen species metabolism (oxalate oxidase), and the octadecanoid pathway (lipoxygenase and allene oxide synthase), while no upregulation was recorded for gene markers of the phenylpropanoid pathway (phenylalanine ammonia-lyase and chalcone synthase). Interestingly, the quantification of 83 metabolites from major chemical families using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in both non-infectious and infectious conditions showed no substantial changes in wheat metabolome upon ulvan treatment, suggesting a low metabolic cost associated with ulvan-induced resistance. Our findings provide evidence that ulvan confers protection and triggers defense mechanisms in wheat against Z. tritici without major modification of the plant physiology.