Hematological Indices Research Articles

Aquaculture potential of Atlantic wolffish (Anarhichas lupus): stress and physiological responses to acute handling.

The Atlantic wolffish (Anarhichas lupus) is a cold-water species with the potential to diversify aquaculture in Northern countries. Few studies have investigated the stress physiology of Atlantic wolffish, and the current knowledge on stress in wolffish species is largely derived from the closely related spotted wolffish (Anarhichas minor). In the current study, Atlantic wolffish were exposed to handling stress mimicking common husbandry conditions in aquaculture such as repeated air exposure and net-chasing. Samples were taken prior to stress exposure (pre-stress; control) as well as 5- and 24-h post-stress. A series of primary and secondary acute stress response parameters were assessed: plasma cortisol, glucose and lactate levels, hematological indices (hemoglobin, Hb; hematocrit, Hct; mean corpuscular hemoglobin concentration, MCHC), and osmoregulatory capacity through plasma osmolality and gill Na+/K+ATPase (NKA) activity. Other secondary stress responses with implications for fish health and welfare are intestinal integrity and transport functions. These parameters were assessed using the Ussing chamber technique. The cortisol peak values were low in comparison to other fish species studied after acute handling stress and occurred as late as 24h post-stress, suggesting that Atlantic wolffish is a slow and low cortisol responder. Plasma glucose remained stable, whereas lactate concentrations significantly decreased between 5 and 24h after stress. There was no effect on pH, Hb, or Hct, although a significant increase in MCHC was found after 5h and 24h, originating from a small increase in Hb. This result suggests a minor increase in Hb synthesis after stress exposure. The intestinal integrity and transport functions as well as gill NKA-activity remained unchanged after stress exposure. In conclusion, Atlantic wolffish appears to exhibit a relatively moderate stress response, characterized by a slow and low primary stress response and minimal secondary effects following husbandry-related acute stress. These findings contribute to the understanding of the species' potential for development as a candidate for marine, cold-water aquaculture.

Associations between neonicotinoids and inflammation in US adults using hematological indices: NHANES 2015-2016.

Toxicological studies suggest neonicotinoids increase oxidative stress and inflammation, but few epidemiological studies have explored these effects. National Health and Nutrition Examination Survey (NHANES) 2015-2016 data were used to estimate associations between neonicotinoid exposure and inflammatory markers, including the C-reactive protein-to-lymphocyte count ratio (CLR), monocyte-to-high-density lipoprotein ratio (MHR), monocyte-to-lymphocyte ratio (MLR), neutrophil-to-lymphocyte ratio (NLR), derived NLR (dNLR), lymphocyte-to-monocyte ratio, platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) using linear and multinomial logistic regression models. Sex was evaluated as a potential modifier. Detection of any parent neonicotinoid (β = -0.62, 95% confidence interval [CI] = -0.98, -0.26) and imidacloprid (β = -0.48, 95% CI = -0.87, -0.10) was associated with decreased CLR. Clothianidin was linked to reduced MLR (β = -0.04, 95% CI = -0.07, -0.02), but increased lymphocyte-to-monocyte ratio (β = 0.52, 95% CI = 0.27, 0.77). Higher dNLR (β = 0.85; 95% CI = 0.26, 1.43) was noted with detection of any neonicotinoid metabolite. Moderately high PLR was observed with detection of any neonicotinoid metabolite (relative risk ratio [RRR] = 1.63, 95% CI = 1.27, 2.09) or 5-hydroxy-imidacloprid (RRR = 2.19, 95% CI = 1.40, 3.41). Sex-modified analyses showed positive associations in males and inverse associations in females for MHR (P int = 0.099, clothianidin), PLR (P int = 0.026, clothianidin), and SII (P int = 0.056, any parent neonicotinoid; P int = 0.002, clothianidin), while the opposite pattern was noted with CLR (P int = 0.073, any parent neonicotinoid) and NLR (P int = 0.084, clothianidin). Neonicotinoids may be associated with inflammatory changes, with potential sexual dimorphism. Further studies are required to explore these findings.

Subacute Toxicity Study of Stem Extract of Telfairia occidentalis in Rats

Telfairia occidentalis Hook (Cucurbitaceae) is used in Ibibio ethnomedicine for the treatment of diverse diseases such as malaria, diabetes and gastrointestinal disorders. Evaluation of subacute administration of ethanol stem extract of Telfaira occidentalis for effect on hematological indices, liver and kidney functions as well as lipid profile of rats was carried out. The stem extract (200, 400, 600 mg/kg body weight) was orally administered to Wistar rats daily for 30 days and the rats were sacrificed under light diethyl ether anesthesia at the completion of the administration. Subacute administration of T. occidentalis stem extract resulted in insignificant increase in body weights of rats especially at high doses without any significant (p>0.05) effect on the weights of heart, brain, liver, kidney, spleen and testes when compared to control, but the weights of ovary was decreased significantly (p<0.01) at higher doses (400 and 600 mg/kg) relative to control. The stem extract treatment did not affect the WBC, RBC and platelets counts, hemoglobin concentration, lymphocytes, monocytes, eosinophil and basophil percentages as well as bleeding and clotting times significantly (p>0.05) relative to control. However, significant (p<0.05) increase of neutrophils percentage at low dose (200 mg/kg) and decreased PCV percentage at higher doses (400 and 600 mg/kg) when compared to control were recorded. The stem extract administration did not cause any significant (p>0.05) effect on total protein, albumin, ALP, total and conjugated bilirubin levels of rats except significantly (p<0.05-0.01) elevated ALT and AST levels at low dose (200 mg/kg) of the extract when compared to control. The stem extract did not cause any significant effect (p>0.05) on creatinine, potassium, sodium, chloride and bicarbonate levels of the treated rats except significantly (p<0.001) elevated urea level at low dose of the extract (200 mg/kg), but caused dose-dependent and insignificant (p>0.05) decreases in total cholesterol, triglyceride, HDL, LDL and VLDL levels when compared to control. The stem extract exerted mild to moderate effect on the histologies of brain, heart, spleen, livers, kidneys, testes and a severe effect on ovaries of rats. Chronic study is advocated to investigate the effect of prolonged administration of the stem extract organs and systems of rats.

Effectiveness of the use of a plant immunostimulator for feline bordetelliosis

In veterinary practice, veterinary specialists solve the important problem of the occurrence and development of immunodeficiency during respiratory infectious pathology in cats with the help of biologically active additives, probiotics, enzymes, immunostimulators and vitamins. Interferons are one of the most popular in use from the list of endogenous immunomodulators. But often the disadvantage of purely immunomodulatory drugs is their difficult transfer and the presence of a negative effect on the body of domestic animals. Therefore, an alternative to interferons in order to increase the immune resistance of the body and improve the well-being of cats is the search, development and use of preparations based on plant raw materials. The article presents the results of the study of the effectiveness of the herbal immunomodulator in comparison with the interferon drug "Anfluron", which was used in two complex treatment schemes for cats suffering from Bodetheliosis infection. To increase the immune resistance of cats for bordeteliosis, an aqueous solution of a medicinal composition based on the Rhodiola rosea root, Oplopanax root, Rozae fruits, Urtica dioica, Crataegus fruits, Hypericum perforatum fruits was used in an effective ratio of 1:1:1:0.8:0.8:0.5. As a result of the use of the specified herbal preparation, a positive trend in the reduction of the manifestation of clinical signs in sick cats was established from the 6th day of use with a direct correlation of the decrease in the manifestation of clinical signs in sick animals treated with a classic interferon drug. As a result of the application of complex therapeutic regimens using various immunomodulatory drugs, a significant alignment of hematological indicators to the physiological norm was noted on the 10th and 14th day of treatment in animals of both study groups. The results obtained in the comparative aspect showed that the proposed herbal preparation has an immunostimulating effect, improves the general condition and can be used in the complex treatment of cats with bordetelliosis.

Impact of Dietary Inclusion of Rice Distillers Dried Grains with Solubles (DDGS) on Hematological Parameters of Commercial Broiler Chicks

An experiment was conducted to evaluate the impact of dietary inclusion of rice-derived distillers dried grains with solubles (DDGS) on the hematological parameters of commercial broiler chicks. A total of 2250 day-old broiler chicks were randomly assigned to treatment groups, each with five replicates. The birds in the control group were fed a basal diet fulfilled with the all nutrient requirements. The experimental diets contained rice DDGS at inclusion levels up to 16 per cent, respectively. At the end of the experiment, blood samples were collected from one bird per replicate for hematological analysis. The results showed significant differences (P<0.05) in red blood cell (RBC) count, packed cell volume (PCV) and hemoglobin levels between the control and DDGS-treated groups, although the numerical differences were minimal. Non-significant differences (P>0.05) in white blood cell (WBC) count and differential leukocyte counts between the control and DDGS-treated groups were observed. These findings revealed that the inclusion of rice DDGS up to 16% in broiler diets does not negatively affect key hematological indices. It was concluded that rice DDGS can be incorporated into broiler diets at inclusion levels up to 16% without adverse effects on hematological parameters.

Prevalence and Haematological Indices of Malaria Parasitaemia among Pregnant Women in Ekpoma, Edo State, Nigeria

Prevalence and Haematological Indices of Malaria Parasitaemia among Pregnant Women in Ekpoma, Edo State, Nigeria

Cardiovascular Complications of Immune Effector Cell Therapies in Pediatric Hematological and Solid Tumors.

Immune effector cell (IEC) therapies, including chimeric antigen receptor (CAR)-modified T-cell therapy, have shown efficacy in pediatric B-cell acute lymphoblastic leukemia (B-ALL) and are being investigated for other malignancies. A common toxicity associated with IEC therapy is cytokine release syndrome (CRS), which can lead to cardiovascular decompensation due to systemic inflammation. Data are limited regarding cardiovascular adverse effects in children. This study aims to describe the cardiovascular adverse effect profile of IEC therapies in pediatric patients with hematologic and solid tumor malignancies. We retrospectively reviewed patients who received IECs directed towards various targets in patients with hematologic, solid, and brain tumor malignancies from January 2014 to June 2023 at Texas Children's Hospital. The primary end point was hypotension requiring vasoactive support and/or heart failure within 30 days of infusion. A total of 203 patients met inclusion criteria. Pretreatment echocardiogram was available for 142 (70%) pediatric patients, of whom 140 (96%) had normal baseline systolic function. Hypotension requiring vasoactive support occurred in 26 (13%) patients. Hematologic malignancy indications (p = 0.002), total body irradiation (TBI) (p = 0.002), and allogenic hematopoietic stem cell transplants (HCT) (p = 0.035) were associated with increased risk of hypotension requiring vasoactive support. Follow-up echocardiograms were available for 14 patients who met the primary end point, and all showed return to baseline within 6 months. Significant hemodynamic compromise occurred in a minority of patients treated with IEC therapies. All experiencing cardiac dysfunction had recovery of function, and there was no cardiovascular-related mortality.

MiRNAs and Hematological Markers in Non-Alcoholic Fatty Liver Disease-A New Diagnostic Path?

Background: Asymptomatic liver steatosis constitutes an emerging issue worldwide. Therefore, we decided to explore relationships between selected types of microRNAs (miRNAs), serological markers of liver fibrosis and hematological parameters in the course of non-alcoholic fatty liver disease (NAFLD). Methods: Two hundred and seven persons were included in the survey: 97 with NAFLD and 110 healthy controls. Serological concentrations of miR-126-3p, miR-197-3p, and miR-1-3p were measured in all participants. Direct indices of liver fibrosis [procollagen I carboxyterminal propeptide (PICP), procollagen III aminoterminal propeptide (PIIINP), platelet-derived growth factor AB (PDGF-AB), transforming growth factor-α (TGF-α) and laminin] together with indirect markers (AAR, APRI, FIB-4 and GPR) were also evaluated. The assessment of hematological parameters concerned: mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), red blood cell distribution width (RDW), MPV to platelet (PLT) ratio (MPR), RDW to PLT ratio (RPR), neutrophil to lymphocyte (LYM) ratio (NLR), PLT to LYM ratio (PLR) and RDW to LYM ratio (RLR). Additionally, the NAFLD fibrosis score and BARD score were applied. Results: The concentration of miR-126-3p and miR-1-3p was higher, and miR-197-3p was lower in the NAFLD group (p < 0.0001). miR-197-3p correlated notably with hematological indices: negatively with PDW (p < 0.05) and positively with PLR (p < 0.05). Conclusions: Significant correlations between miRNA molecules and hematological markers in the course of NAFLD indicate inflammation as a potential background and create new possibilities for a diagnostic approach.

Chia (Salvia hispanica) Seed Oil Modulates the Haemato-Immunological Response, Antioxidative Status and Cytokine Gene Expression of Tropical Freshwater Teleost, Labeo rohita.

The present investigation attempts to evaluate the impact of the dietary inclusion of chia (Salvia hispanica) seed oil (CSO) on the indices of haemato-immunology, metabolic enzymes, and expression of immune-responsive cytokine genes in Labeo rohita (rohu) fingerlings. The responses were observed in a 60-day feeding trial, set up with a total of 180 rohu fingerlings (19.74 ± 0.33 g) randomly allocated to four treatment groups with three replicates each. The groups were fed with a basal diet incorporated with 0%, 1.0%, 2.0%, and 3.0% CSO, denoted as control, CSO (1), CSO (2), and CSO (3), respectively. Significant (p < 0.05) augmentation of hematological indices such as total protein and globulin levels was observed in the group fed a 1.0% CSO-supplemented diet. Serum glucose, cholesterol, triglycerides, and complement reactive protein levels declined, whereas marker anti-oxidative enzymes (SOD, CAT, and GST) and protein metabolic enzymes (ALT and AST) increased (p < 0.05) in the lowest CSO-supplemented group. A significant upregulation of inflammatory cytokine viz. IL-1β, IFN-γ, TNF-α, and TLR22 alongside downregulation of IL-10 was noted in various tissues. The results support the inclusion of 1.0% CSO as a prospective dietary vegan substitute to fish oil in rohu aquaculture.

Assessment of Glyphosate Roundup Turbo (450 g/l) on Haemato-biochemical Indices of Freshwater African Catfish (Clarias gariepinus) Fingerlings

The escalating use of glyphosate-based herbicides in agricultural practices has sparked concerns regarding their potential ecological impact. This study undertakes a comprehensive investigation into the acute ecotoxicological effects of a brand-new glyphosate (Roundup Turbo 450 g/l) on haematology and biochemical indices of Clarias gariepinus a commercially vital fish species in Nigeria. Fish were exposed to 0.0 as control, 0.5, 0.7, 0.9 and 1.2 mg/l glyphosate for 96 hours in the laboratory. Water quality parameters monitored were temperature, pH, dissolved oxygen electrical conductivity, nitrate and nitrite. The blood, liver and gills were extracted and analyzed. Haematological and biochemical analyses revealed significant changes (P &lt; 0.05) as concentration increases. The decrease in red blood cell, pack cell volume, haemaglobin and white blood cell was indicative of glyphosate-induced hematotoxicity. Biochemical analyses disclosed alterations in enzymatic activities within liver and gill tissues, including significant increase in alanine aminotransaminases, aspartate aminotransferase and alkaline phosphatase, suggesting hepatic and branchial dysfunction and decrease in total protein, globulin and albumin, showing enhanced disruption in protein synthesis. Glucose and albumin in liver were not detected. Behavioral observations indicated the fish heightened stress response, characterized by erratic swimming patterns, increased agitation, equilibrium loss, hyperactivity and skin erosion. The 96-hour LC50 value for this study was 2.50 mg/l and it highlights glyphosate roundup turbo’s potential to exert detrimental effects on Clarias gariepinus populations. These findings underscore the imperative for sustainable agricultural practices, judicious pesticide management, and rigorous environmental monitoring to mitigate the ecological risks associated with glyphosate use.

Study on Hematological and Biochemical Index of Blood and Vitreous Humor in the Celestial-Eye Goldfish

In this study, we conducted an exploration of blood collection, hematological and biochemical comparative analysis of the unique vitreous humor and blood of the celestial-eye goldfish (CE) (Carassius auratus), and obtained hematological and biochemical indicators for healthy celestial goldfish blood. After exploring the optimal method for collecting blood and ocular fluid, blood and vitreous humor samples were drawn from healthy CE, and an automatic hematology analyzer was used to measure 17 physiological indicators in the blood and vitreous humor. Additionally, 20 biochemical indices were measured by an automatic biochemical analyzer. Experiments were also conductedto evaluate the effect of blood and vitreous humor collection on the subsequent survival rate of the CE. The results showed that the blood of CE exhibited values for 17 hematological indicators, including red blood cells (mean value: 2.19 × 1012/L), white blood cells (mean value: 62.21 × 109/L), hemoglobin (mean value:138.25 g/L), and no eosinophils or basophils. We also found that the levels of aspartate amino transferase (AST) and glutamyl transpeptidase (GGT) in the vitreous humor of CE were significantly higher than those in the serum (p &lt; 0.01). No cells were detected in the vitreous humor of CE, and there was no significant difference in survival rate compared to normal goldfish after extracting blood and vitreous humor from CE (p &gt; 0.05). This study provides fundamental data for the cultivation of healthy goldfish, the development of goldfish hematology, and further research on the development and formation of CE’s unique eye traits.

Protective effects of myrtenol against paraquat-induced toxicity in rats

BackgroundParaquat (PQ) is a widely used pesticide, can cause severe intoxication and respiratory failure. Myrtenol (Mrl), an essential oil derived in various plants, exhibits several biological properties, including anti-inflammatory and antioxidant activities. This study aims to investigate the protective potential of Mrl against oxidative stress and inflammation caused by PQ exposure.MethodsTwenty-five Wistar albino rats were divided into the following groups (n = 5 in each group): a control group (treated by dimethyl sulfoxide (DMSO)), a PQ group (exposed to 54 mg/m³ aerosol PQ), and two treatment groups that were exposed to PQ aerosol and administered oral Mrl at doses of 25 mg/kg/day and 50 mg/kg/day, respectively. The final group was exposed to PQ aerosol and treated with oral dexamethasone at a dose of 0.03 mg/kg/day. Various hematological, oxidative, inflammatory, and pathological indices were measured at the conclusion of the treatment period.ResultsPQ decreases the levels or activities of superoxide dismutase (SOD), catalase (CAT), and Thiol, while increasing the levels or activities of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and malondialdehyde (MDA). Mrl restored activites of SOD, and CAT, as well as thiol levels to near-control values while reducing TNF-α, IL-6, and MDA levels. Pathological studies further confirmed the therapeutic effects of Mrl.ConclusionThe results of this study demonstrate the promising therapeutic effects of Mrl against inhaled PQ in rats.

Predictive value of preoperative circulating tumor cells combined with hematological indexes for liver metastasis after radical resection of colorectal cancer.

Colorectal cancer is one of the most common malignant tumors in the world, and about 50% of its advanced patients will have liver metastasis. Preoperative assessment of the risk of liver metastasis in patients with colorectal cancer is of great significance for making individualized treatment plans. Traditional imaging examinations and tumor markers have some limitations in predicting the risk of liver metastasis. Therefore, it is of great clinical value to explore more sensitive and specific predictive indicators for improving early detection and treatment effect. In recent years, circulating tumor cells (CTCs), as a new biomarker, have attracted much attention because of their close relationship with tumor metastasis and prognosis. The purpose of this study is to collect and analyze the data of colorectal cancer patients treated in our hospital, so as to determine the predictive value of circulating tumor cells before operation and related hematological indexes for liver metastasis after radical resection of colorectal cancer, and to establish the corresponding prediction model to provide gastrointestinal surgeons with more accurate identification of high-risk patients and guidance for treatment. A total of 88 patients were included in this study, and 26 of whom developed liver metastasis after colorectal cancer surgery. The possible related factors are included in the single factor logistic regression, and the results are obtained after analysis. Body mass index, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9, tumor marker CA72-4 (CA72-4), cytokeratin-7 (CK-7), CTC count, and neutrophil-to-lymphocyte ratio (P < .2) are risk factors for liver metastasis after radical resection of colorectal cancer. Furthermore, the data obtained were included in multivariate regression analysis, and CEA, CA72-4, CK-7, and CTC counts were independent risk factors for liver metastasis after radical resection of colorectal cancer (P < .05). This study confirmed that CEA, CA72-4, CK-7, and CTC counts are independent risk factors for liver metastasis after radical resection of colorectal cancer. In addition, the prediction model of this study can help gastrointestinal surgeons accurately identify patients who are prone to liver metastasis after colorectal cancer surgery.

Correlation between the white blood cell/platelet ratio and 28-day all-cause mortality in cardiac arrest patients: a retrospective cohort study based on machine learning.

This study aims to evaluate the association between the white blood cell-to-platelet ratio (WPR) and 28-day all-cause mortality among patients experiencing cardiac arrest. Utilizing data from 748 cardiac arrest patients in the Medical Information Mart for Intensive Care-IV (MIMIC-IV) 2.2 database, machine learning algorithms, including the Boruta feature selection method, random forest modeling, and SHAP value analysis, were applied to identify significant prognostic biomarkers. Key patient characteristics, encompassing demographic data, comorbidities, hematological and biochemical indices, and vital signs, were extracted using PostgreSQL Administration Tool (pgAdmin) software. The Cox proportional hazards model assessed the impact of WPR on mortality outcomes, while Kaplan-Meier survival curves and restricted cubic spline (RCS) analysis further validated the findings. Subgroup analyses stratified the prognostic value of WPR by demographic and clinical factors. WPR demonstrated the highest prognostic significance among the variables studied, showing a strong association with 28-day all-cause mortality. In the unadjusted Model 1, hazard ratios (HRs) for WPR quartiles ranged from 1.88 (95% CI: 1.22-2.90) in Q2 to 3.02 (95% CI: 2.04-4.47) in Q4 (Ptrend <0.05). Adjusted models (Models 2-4) confirmed the robustness of these associations, even after accounting for demographic and clinical covariates. Kaplan-Meier and RCS analyses revealed a significant U-shaped relationship between WPR and mortality risk. Subgroup analyses indicated that elevated WPR was particularly associated with increased mortality in males, elderly patients, married individuals, and those with chronic pulmonary disease. WPR serves as an independent and reliable prognostic biomarker for 28-day mortality in cardiac arrest patients. Its integration into clinical decision-making may enhance the early identification of high-risk patients and guide tailored therapeutic interventions.

Metabolic stimulation improves bioenergetics and haematologic indices of circulating erythrocytes from sickle cell mice.

Circulating mature red blood cells (RBCs) from patients and mice with sickle cell disease (SCD) abnormally retain mitochondria, a factor shown to contribute to the disease's pathobiology. To further understand the functional implications of RBC mitochondria retention in SCD, we used mitochondria inhibitors and metabolites/substrates from the tricarboxylic acid cycle, oxidative phosphorylation and glycolysis pathways (ADP, glutamate, malate, pyruvate, succinate or all metabolites combined) and examined RBC bioenergetics, reactive oxygen species (ROS) levels, calcium flux and hydration. In RBCs from sickle mice, mitochondria inhibition reduced ATP levels by 30%-60%, whereas control RBCs were unaffected. Conversely, in vitro treatment with metabolites/substrates known to stimulate mitochondria function increased RBC ATP levels and reduced RBC ROS, and these effects were notably more pronounced in sickle RBCs compared to those in control mice. In sickle RBCs, the increases in ATP and decreases in ROS were associated with greater decreases in RBC baseline calcium concentration and improvements in calcium flux. These changes also led to greater increases in RBC volume and hydration, and greater decreases in mean corpuscular haemoglobin concentration in sickle RBCs compared to control RBCs. These results propose a novel model of RBC metabolism in SCD, where retained mitochondria in circulating RBCs are functional and can significantly impact RBC bioenergetics, ATP production and RBC hydration and redox status. These in vitro findings could inform the development of in vivo strategies aimed at increasing RBC ATP, reducing RBC ROS, improving RBC hydration, which could decrease sickling and prove beneficial in SCD. KEY POINTS: Red blood cells (RBCs) from humans and mice with sickle cell disease (SCD) abnormally retain mitochondria. Mitochondria inhibitors lower ATP in circulating RBCs from sickle mice indicating that retained mitochondria contribute to ATP levels in sickle RBC. Metabolic stimulation of mitochondria function improves RBC bioenergetics, redox state and calcium flux, and increases RBC hydration. These data propose a new model of RBC metabolism in SCD, where in addition to glycolysis, retained mitochondria contribute to RBC ATP production. The effects of metabolic stimulation of RBCs resulting in improved RBC bioenergetics and hydration may prove beneficial in SCD.

A multi-centre UK-based survey on angioedema secondary to acquired C1 inhibitor deficiency.

Acquired angioedema due to C1-inhibitor deficiency (AAE-C1-INH) is very rare compared to its prototype, hereditary angioedema. An updated characterisation of the AAE-C1-INH cohort in UK is required to inform management. To describe the disease burden of AAE-C1-INH, long-term prophylaxis (LTP) and the clinical, immunochemical and treatment profiles of AAE-associated diseases in UK. Retrospective data on 117 AAE-C1-INH patients were collected using a national survey proforma across 25/34 Adult Clinical Immunology and Allergy centres in UK. Other European cohorts were compared. Median age at AAE-C1-INH diagnosis was 65 years with 3.4% of patients diagnosed below 40 years. The median delay in diagnosis was one year. Antifibrinolytics and attenuated androgens showed comparable efficacy as LTP 88.9% and 89.5%, respectively. A haematological disorder was identified in 83.8% AAE-C1-INH patients compared to 3.4% autoimmune diseases. The predominant haematological disorders were splenic marginal zone lymphoma (SZL) 34% followed by MGUS 16%. The severity of angioedema did not depend on the associated disease. Anti-C1INH-autoantibodies testing was limited at 23.1%. Rituximab monotherapy was effective in treating 9/9 SZL and 1/2 MGUS-associated AAE-C1-INH. Rituximab efficacy was independent of anti-C1INH-autoantibodies detection with response in 3/3 seronegative and 4/4 seropositive patients. The diagnosis of AAE-C1-INH should not be overlooked below the age of 40 years. The choice of oral LTP should be informed by propensity to side-effects. B-cell depletion could be considered in treating monoclonal B cell disorder-associated-AAE-C1-INH in the absence of haematological indications. Further studies are required to address the clinical utility of anti-C1INH-autoantibodies.

Early hematological indices as a predictor of placenta accreta in women with high suspicion of accreta

There is a lack of early biomarkers to predict the placenta accreta spectrum; thus, searching for available and easily obtained markers such as hematological indices is an attractive option. The current study is a diagnostic accuracy study included 198 women; all women underwent an assessment of their hematological indices during their first trimester as part of their routine antenatal care. All women included in the study had a high suspicion of developing placenta accreta spectrum; the women were followed up until their delivery. White blood cell, neutrophil count, and red cell distribution width (RDW) were significantly higher in the women with accreta than those without accreta. RDW had fair ability (area under the curve, 0.707) as a predictor of accreta. RDW had the highest positive and lowest negative likelihood ratios (indicating better value than the rest of the markers). In multivariate analysis, RDW and the platelet-lymphocyte ratio were independently associated with accreta after adjustment to the effects of age, gestational age, gravida, parity, abortion, and past medical/surgical history. In conclusion, simple, early blood count parameters may be utilized for placenta accreta; RDW appears to be the best predictor of placenta accreta.

Impact of Agroindustrial Waste Fermented with Bacteria and Yeasts and Their Effect on Productive, Hematological, and Microbiota Indicators in Guinea Pigs (Cavia porcellus)

In the last decade, the production of guinea pig meat in Andean countries has increased due to the growing number of consumers of this meat. Objective: To evaluate the effect of including different doses (0.50, 1.00, and 1.50 mL) of agro-industrial substrates (molasses distillery waste) fermented with lactic acid bacteria and yeasts on productive performance, hematological profile, relative weight changes in digestive tract organs, and changes in the intestinal microbiota in guinea pigs (Cavia porcellus). Materials: A total of 300 guinea pigs, Kuri breed, aged 20 days and weighing 330 g, were distributed into 10 groups of 30 animals each. Ctrl, Control. La, substrate fermented with Lactobacillus acidophilus (8.1 × 107 CFU/mL). Kf, substrate fermented with Kluyveromyces fragilis (7.4 × 106 CFU/mL). La + Kf, substrate fermented with bacteria and yeasts; the evaluated doses were 0.50, 1.00, and 1.50 mL/animal. The indicators evaluated in the study included weight gain, health, hematological profile, relative weight of digestive tract organs, and changes in the intestinal microbiota. Results: The parameters evaluated were toxicity, productive parameters, occurrence of diarrhea and mortality, and blood profile. The results showed a significant increase in the weight of the animals consuming probiotics, especially at higher doses. Additionally, an improvement in the intestinal microbiota was observed, with an increase in beneficial bacteria such as Lactobacillus and a decrease in pathogenic bacteria. Probiotics also influenced the hematological parameters and the weight of digestive tract organs, suggesting a positive effect on the overall health of the animals. Conclusions: Supplementation with probiotics proved to be a promising strategy for improving productive performance and intestinal health in guinea pigs. Supplementation with L. acidophilus and K. fragilis significantly enhances guinea pig growth and modulates the intestinal microbiota. The combination of strains and appropriate doses maximizes benefits. These results promise applications in animal production, requiring further studies to confirm their efficacy in other species and developmental stages.

Safety and efficacy of oral edible bird’s nest supplementation: Anti-inflammatory and immunomodulatory benefits for Arabian race stallions during exercise

Background and Aim: Exercise-induced oxidative stress and inflammation adversely affect the health and performance of athletic horses. This study aimed to evaluate the safety of edible bird’s nest (EBN) supplementation and its potential anti-inflammatory and immunomodulatory effects in Arabian race stallions during exercise. Key objectives included assessing EBN’s impact on hepatic and renal function, hematological profiles, and sialic acid (SA) levels. Materials and Methods: Two experiments were conducted on 18 Arabian stallions. In Experiment 1, six healthy stallions were divided into control (n = 2) and EBN-supplemented groups (n = 4) to assess safety. The treatment group received 10 g of EBN daily for 12 days. Vital signs, hematological parameters, and organ function markers were monitored. In Experiment 2, 12 stallions were divided into three groups: EBN (n = 3), Premier E® (n = 3), and control (n = 6). The exercise regimen included a daily 10-min walk, 10-min canter (30 km/h), and 10-min walk for 30 days. Blood samples were analyzed for hematological indices and SA levels pre- and post-exercise. Results: In Experiment 1, EBN supplementation demonstrated no adverse effects, maintaining normal hematological and vital parameters. Kidney and liver function tests revealed significantly reduced creatine kinase, total bilirubin, and aspartate aminotransferase levels in the EBN group. Experiment 2 showed higher SA levels post-exercise in the EBN group (p &lt; 0.05) compared to Premier E® and control groups, suggesting enhanced anti-inflammatory response. Hematological indices, including mean corpuscular volume, red cell distribution width, and platelet counts, were significantly improved in the EBN group, indicating potential immunomodulatory benefits. Conclusion: EBN supplementation is safe and offers anti-inflammatory and immunomodulatory effects in Arabian race stallions, reducing exercise-induced hepatic and muscular stress markers while enhancing recovery. These findings support EBN as a valuable dietary supplement for performance horses. Further studies should explore long-term effects and molecular mechanisms to optimize its use in equine sports. Keywords: anti-inflammatory, arabian race stallions, edible bird’s nest, exercise stress, immunomodulatory.

The Clinical Value of the Combined Detection of Systemic Immune-Inflammation Index (SII), Systemic Inflammation Response Index (SIRI), and Prognostic Nutritional Index (PNI) in Early Diagnosis of Gastric Cancer.

Gastric cancer (GC) is a common malignant tumor of the digestive tract. Accumulating studies suggest that inflammation is linked with the pathogenesis of GC. The study delves into novel hematological inflammatory markers, such as systemic immune-inflammation index (SII), systemic inflammation response index (SIRI), and prognostic nutritional index (PNI), to explore their potential applications in early diagnosis of GC. From October 2020 and August 2024, 1339 GC patients admitted to our hospital were enrolled in this study. The pre-treatment SII, SIRI, and PNI was calculated from peripheral blood samples. Univariate and multivariate logistic regression analyses were utilized to verify independent risk factors for patients, and constructed the nomograms. The correlation between hematological indicators and tumor-node-metastasis (TNM) stage was assessed through Spearman's analysis. Eligible patients and healthy controls were grouped by gender. The diagnostic ability of PNI was significantly superior to other indicators to diagnose male GC (area under the curve [AUC]=0.908, 95% CI: 0.892-0.925) and female GC (AUC=0.890, 95% CI: 0.865-0.914). Besides, the combination of hematological indicators is more effective in diagnosing GC patients, especially for male patients (AUC=0.916, 95% CI: 0.901-0.932, sensitivity: 84.98%, specificity: 84.29%). The C-statistic of Nomogram model was 0.917 for males and 0.875 for females. In both male and female cohorts, CEA, SII, and SIRI were positively correlated with TNM stage, while PNI was negatively correlated. The AUC of CEA, SII, SIRI, and PNI combined for the diagnosis in the early stage of male GC patients was 0.897 (95% CI: 0.875-0.918, sensitivity: 86.57%, specificity: 80.30%) is higher than that of in the advanced stage (AUC: 0.745, 95% CI: 0.710-0.780, sensitivity: 56.53%, specificity: 82.86%). The combined CEA, SII, PNI, and SIRI could be used as screening biomarkers in diagnosing GC, especially in the early stage of male GC patients.