Heavy Infestation Research Articles

English

The production of groundnut is constrained by several factors, among which is Aspergillus spp. In addition to causing quantitative losses, Aspergillus spp. produce highly toxic and carcinogenic chemical substances known as aflatoxins. This study was conducted with the objectives to (i) identify Aspergillus species associated with groundnuts, (ii) determine the frequency of seed contamination, and (iii) survey agro-ecological conditions related to groundnut contamination by Aspergillus spp. About 270 groundnut samples were collected from farmers’ storage, fields and local markets of three districts that is, Babile, Darolabu and Gursum of Eastern Ethiopia for mycological analysis in the year 2010. Results of the mycological analysis suggested heavy infestation of groundnut samples by various molds including Aspergillus niger, Aspergillus flavus, Aspergillus ochraceus, Aspergillus parasiticus and Pencillium species. At the district level, the incidence of infected groundnut kernels ranged from 50 to 80%. Within the district kernel infection varied between 36.3 and 100%. The common Aspergillus symptoms (yellowing or chlorotic leaves, wilting, drying and brown or black mass covered by yellow or greenish spores) were also observed in groundnut fields. The current results were consistent with our earlier report of heavy aflatoxin contamination of groundnut from the same places, suggesting the urgent need to apply control measures against toxigenic fungi and associated mycotoxins. Key words: Aspergillus spp., groundnut, Penicillium spp., Ethiopia.

Field Distribution of <I>Dasineura oxycoccana</I> (Diptera: Cecidomyiidae) Adults, Larvae, Pupae, and Parasitoids and Evaluation of Monitoring Trap Designs in Florida

Blueberry gall midge, Dasineura oxycoccana (Johnson), is a pest of cultivated blueberries throughout the world. Larvae feed and develop in developing leaf buds, and also in flower buds of rabbiteye blueberries, which causes buds to fall off the plant. These injuries can cause up to 80% yield loss in heavy infestations. As the larvae are protected from insecticides, adults must be targeted with foliar applications. Consequently, the detection of adults through an effective monitoring program is critical to time insecticide sprays against the blueberry gall midge. Understanding the distribution of the midge and its parasitoids is also important information for developing a more effective pest management program. A comparison of three monitoring trap types demonstrated that bucket emergence traps and clear panel traps captured similar numbers of midges, although the bucket trap is more sensitive at low population levels. Using bucket emergence traps, we found that nearly 80% of the midges collected pupated within 48 cm of the blueberry bush, suggesting that a targeted soil treatment may be a viable integrated pest management tactic that could be included in a midge management program. Traps and bud samples demonstrated that adult and larval midges and parasitoids were randomly distributed throughout the field in both years, with the exception of larval aggregation in early 2012. As parasitoid distribution is parallel to host occurrence within blueberry plantings, this increases the potential for biological control activities against the blueberry gall midge in fields that do not receive broad-spectrum insecticide applications.

Weed Management in Dry-Seeded Fine Rice under Varying Row Spacing in the Rice-Wheat System of Punjab, Pakistan

Direct seeding can curtail water and labor inputs involved in rice production; nevertheless, its large-scale adoption is impeded by heavy weed infestation. A field study was undertaken in 2011 and 2012 to evaluate the effects of crop row spacing (20 and 10 cm) and postemergence herbicides on weed growth and yield of dry-seeded rice. As a herbicide, pyrazosulfuron ethyl at 30 g ha–1, bispyribac-sodium at 30 g ha–1, or penoxsulam at 15 g ha–1 was applied (15 days after sowing, DAS) alone or in combination with fenoxaprop-p-ethyl at 86.25 g ha–1 subsequently (30 DAS). In addition, a partial weedy plot (manually weeded once at 28 DAS), and a weed-free plot were established for each row spacing In the partial weedy plot with 10-cm row spacing, the weed density was only 21 and 25% lower than that in the plot with 20-cm row spacing in 2011 and 2012, respectively. The sole application of an early postemergence herbicide restricted weed growth, while subsequent application of fenoxaprop as late postemergence application suppressed weed growth further, the magnitude of suppression being more pronounced in the plot with narrow row spacing. The density and biomass of weeds were lowest in the plot with 10-cm row spacing applied bispyribac-sodium followed by fenoxaprop . Under weed-free conditions, yields were 29% higher in the plot with 10-cm row spacing (4.18 t ha–1) than in that with 20-cm row spacing (3.23 t ha–1). Grain yield in the herbicide-treated plots was 87 – 188% higher than that in the partial weedy plots. These results suggested that narrow row spacing and sequential herbicide application can help tackle recalcitrant weed flora in dry-seeded rice fields.

Early grain storage research

Storage problems Survey respondents were asked to choose and rank the three most important rough rice storage problems they experi- enced during the past 5 years from a list of six potential problems (fig. 1). For each respondent, the problem ranked as most important, second most important, and third most important received a score of 3, 2 and 1, respectively, and problems that were not ranked received a score of 0. Then, for each problem, the average and relative scores were calculated. A prob- lem’s relative score was determined by di- viding the problem’s average score by the highest possible average score (3). For on- farm operations, maintaining appropriate grain moisture is one of the most impor- tant problems respondents faced, fol- lowed by insects and grain temperature. For off-farm operations, one of the most Early grain storage research “Many insects that infest grain in farm storage are small. Some are smaller than a grain of wheat. In fact, with some species, a single kernel of grain furnishes sufficient food for the development of from one to several individuals. “Among the more important pests are the granary weevil, rice weevil, lesser grain borer, Angoumois grain moth, confused flour beetle and the saw- toothed grain beetle. The first four mentioned are capable of attacking and destroying sound grain. The others generally feed upon broken grains, particu- larly the finer particles. “Where the environment is favor- able, these insects cause serious dam- age and under extreme conditions the grain may be completely destroyed. Most of the important grain pests are widespread throughout California and if grain is not properly protected it is subject to heavy infestation. “The development of stored grain pests is largely regulated by tempera- ture and the moisture content of the food on which they feed. The most favorable temperature range is from 80 to 85 Deg. F; while the most ideal moisture content of the food ranges from 13 to 17 per cent.” At the time of this writing, author Abraham E. Michelbacher was assistant professor of entomology and assistant entomologist at the Agricultural Experiment Station at UC Berkeley. He went on to become a full professor and leader in UC Berkeley’s Department of Entomology, as well as a pioneer in the fields of biological pest control and the specifically targeted use of pesticides. After retirement in 1960, he was named professor emeritus and continued his research and Extension work for nearly 30 years more. Michelbacher died in 1991, aged 92. http://californiaagriculture.ucanr.edu • JANUARY–JUNE 2014 41 UC Cooperative Extension Centennial fans manually when conditions are ap- propriate to dry and maintain rice at the desired temperature and moisture con- tent. Alternatively, an aeration controller system can be used to automatically start and stop fans. Our survey shows that aeration controller systems are uncom- mon. Only one on-farm and eight off-farm operations reported having an aeration controller system. Grain temperature can be assessed using hand-held thermometers, tem- perature probes or temperature cables. Hand-held thermometers can measure the temperature of the grain mass surface or of a sample extracted from the grain mass using a grain probe. Temperature probes can be inserted into the grain mass to determine the temperature of the grain at a certain depth. Temperature cables are sensors suspended from the roof of a storage structure that run nearly to the floor of the structure; the number of cables in a structure varies with the structure’s size. Temperature probes and cables allow managers to detect grain temperature changes at different grain depths without having to extract samples from these depths. Most of the operations replying to our survey report monitor- ing grain temperature during storage (see below, “Aeration”). The majority of on-farm operations (n = 23) report using a hand-held thermometer (52.2%), while most off-farm operations (n = 24) report relying on temperature cables within stor- age structures (79.2%).

Perceived Constraints in the Accessibility of Production, Marketing and Processing of Paddy in Mahasamund District of Chhattisgarh

Chhattisgarh is one of the leading Paddy producing state in India contributing 3.6 m ha area and 6.16 mt (2010–11) production. Chhattisgarh state has achieved “Krishi Karman” award from Government of India for the abundant paddy production in year 2010–11. Despite such an achievement, Paddy Growers, Traders and Processors in various districts of the state are not free from the constraints in production, marketing and processing of it. Present study was undertaken in Mahasamund district of Chhattisgarh state during the year 2010. The objective of the study was to rank the identified constraints in production, marketing and processing of the paddy. Data were collected from the selected farmers, trader and processors through personnel interview by using pre structured survey schedule. Results of the study revealed that the heavy infestation of insect pests, problem of high weed occurrence and high labour cost were major constraints in paddy production perceived by the farmers. In marketing of paddy lack of transportation and road facility, lack of regulated market and unremunerative price were observed as severe problems while processing problems perceived by processors of study area as a severe constraints are related to electricity problem and efficiency problem of processing unit.

Cryptotermes brevis (Isoptera: Kalotermitidae) in the Azores: lessons after 2 yr of monitoring in the Archipelago.

The dispersal flights of West Indian drywood termite, Cryptotermes brevis (Walker) (Isoptera: Kalotermitidae) were surveyed in the major cities of Azores. The sampling device used to estimate termite density consisted of a yellow adhesive trap (size 45 by 24 cm), placed with an artificial or natural light source in a dark attic environment. In addition, data from two other projects were used to improve the knowledge about the geographical distribution of the species. The level of infestation in the two main Azorean towns differed, with high levels in the houses of Angra do Heroísmo, whereas in Ponta Delgada, there are fewer houses with high levels of infestation. The infestation in Ponta Delgada shows a pattern of spreading from the center outward to the city’s periphery, whereas in Angra do Heroísmo, there was a pattern of spreading outward from several foci. The heavy infestation observed in Angra do Heroísmo and the clear increase of infestation levels observed from 2010 to 2011 is a reason for concern and calls for an urgent application of an Integrated Pest Management (IPM) control strategy.

Prevalence of Menopon gallinae Linn. (Insecta, Phthiraptera, Menoponidae, Amblycera) upon poultry birds (Gallus gallus domesticus) of selected locality of district Chamoli Garhwal (Uttarakhand), India

Prevalence and intensity of infestation of Menopon gallinae (Linn.) was recorded from 688 poultry birds belonging to 30 selected locality of district Chamoli (Uttarakhand) during March, 2012 to February, 2013, out of which 310 were found infested (45.06%). Prevalence rate were higher in female (46.8%) than male (42%) birds in present investigation. Higher infestation were noted in certain parameters like captivity (larger group), poor health, poor hygienic condition, poor feather condition, black feather colour, desi breed and older birds. Singly reared, good health, good hygienic condition, good feather condition, mix colour, broiler breed and younger poultry birds were found to have least infestation. Intensity of infestation was noted by coding system. Maximum poultry bird bore very light (VL) infestation (29.68%) while very few were found with very heavy (VH) infestation (4.52%). Relative monthly intensity of infestation remained more or less similar in all the categories. Lice were peaked during summer in comparison to the winter. Strong positive correlation existed between monthly lice index and temperature (+0.79); lice index and photoperiod (+0.84), while the same were found insignificant with humidity (-0.23) and rainfall (-0.17).

PM 7/116 (1)Tetranychus evansi

PM 7/116 (1)<i>Tetranychus evansi</i>

Tungiasis – A Janus-faced parasitic skin disease

Tungiasis is a parasitic skin disease caused by the penetration of female sand fleas (Tunga penetrans). It is acquired when people walk barefoot or rest on soil, where sand fleas have completed the off-host cycle. Tungiasis is a classic poverty-associated disease which belongs to the family of neglected tropical diseases (NTD). It has a Janus-face: while in travellers tungiasis usually is a benign self-limiting skin disease, inhabitants of endemic areas suffer from heavy infestations and severe, frequently debilitating and incapacitating morbidity. We describe the epidemiological and clinical characteristics of travel-associated tungiasis and compare these features to the situation in resource-poor communities in South America and sub-Saharan Africa.

A model of the relationship between weedy rice seed‐bank dynamics and rice‐crop infestation and damage in Jiangsu Province, China

A heavy infestation of weedy rice leading to no harvested rice has never been predicted in China due to a lack of knowledge about the weedy rice seed bank. We studied the seed-bank dynamics of weedy rice for three consecutive years and analyzed the relationship between seed-bank density and population density in order to predict future weedy rice infestations of direct-seeded rice at six sites along the Yangtze River in Jiangsu Province, China. The seed-bank density of weedy rice in all six sites displayed an increasing trend with seasonal fluctuations. Weedy rice seeds found in the 0-10 cm soil layer contributed most to seedling emergence. An exponential curve expressed the relationship between cultivated rice yield loss and adult weedy rice density. Based on data collected during the weedy rice life-cycle, a semi-empirical mathematic model was developed that fits well with the experimental data in a way that could be used to predict seed-bank dynamics. By integrating the semi-empirical model and the exponential curve, weedy rice infestation levels and crop losses can be predicted based on the seed-bank dynamics so that a practical control can be adopted before rice planting.

Regression of Severe Tungiasis-Associated Morbidity after Prevention of Re-Infestation: A Case Series from Rural Madagascar

Tungiasis (sand flea disease) is a neglected tropical disease. Heavy infestation results in mutilation of the feet and difficulty in walking. We identified eight individuals with extremely severe tungiasis in rural Madagascar. To prevent reinfestation, four individuals received solid shoes and four received a daily application of an herbal repellent effective against Tunga penetrans. Over a period of 10 weeks the feet were examined and the severity of tungiasis-associated morbidity was measured. Within this period, the severity score for acute tungiasis decreased 41% in the shoe group and 89% in the repellent group. The four major inflammation-related symptoms disappeared in the four patients of the repellent group, but only in two patients of the shoe group. Those observations indicate that cases with extremely severe tungiasis, associated morbidity almost totally disappears within 10 weeks if the feet are protected by a repellent. Wearing shoes reduced acute morbidity only marginally.

First Report of "Candidatus Liberibacter solanacearum" on Tomato in El Salvador.

In April of 2012, tomato plants (Solanum lycopersicum) grown near the town of Yuroconte in the municipality of La Palma, Chalatenango, El Salvador, were observed with symptoms resembling those of "Candidatus Liberibacter solanacearum" infection. The symptoms included overall chlorosis, severe stunting, leaf cupping, excessive branching of axillary shoots, and leaf purpling and scorching (1,2,3). Disease incidence in several fields in the area ranged from 40 to 60%. Heavy infestations of the potato/tomato psyllid, Bactericera cockerelli, were observed in the affected fields and this insect has been shown to transmit "Ca. L. solanacearum" to tomato and other solanaceous species (1,2,3). Leaf samples and psyllids were collected from one of the fields and total DNA was purified from the leaves of 8 and 10 symptomatic and asymptomatic plants, respectively (2,3). DNA was also extracted from the psyllids and the samples were tested by PCR for species confirmation. PCR oligonucleotide primers specific for both 16S rDNA (OA2 and OI2c) and a gene for a surface antigen for the outer membrane protein (OMB) (OMB 1482f and 2086r) of "Ca. L. solanacearum" were used to confirm the presence of the bacterium in infected tomatoes (1). Four of the eight symptomatic tomatoes (50%) tested positive for "Ca. L. solanacearum" using both primer pairs and all asymptomatic plants were negative for the bacterium. The collected psyllids were first identified through a morphological key, then verified using species-specific PCR primers (CO1 F3 and CO1 meltR) that generated a 94-bp fragment that was consistent with DNA from B. cockerelli (4). Amplicons generated with DNA from two plant samples with each primer pair were cloned and four clones of each of the four amplicons were sequenced. BLASTn analysis of the 16S rDNA consensus sequences from the clones (1,168 bp; deposited in GenBank as Accession Nos. KC768318 and KC768319) showed 100% identity to "Ca. L. solanacearum" sequences in GenBank (HM246509 and HM245242, respectively). Two OMB consensus sequences were 98% identical (deposited in GenBank as KC768326 and KC768327) and both sequences were 97 to 100% identical to a number of "Ca. L. solanacearum" sequences in GenBank (e.g., CP002371, FJ914617, JN848754, and JN848752). To our knowledge, this is the first report of "Ca. L. solanacearum" associated with tomato in El Salvador and the first formal report of the bacterium in the country. This bacterium has caused millions of dollars in losses to the tomato industry in New Zealand, Mexico and the United States (2,3). Tomatoes are an economically important commodity in Central America and are severely damaged by "Ca. L. solanacearum" infection. The confirmation of "Ca. L. solanacearum" infections in El Salvador alerts the agricultural sector to the presence of this serious pathogen.

First Report of "Candidatus Liberibacter solanacearum" Associated with Psyllid-Infested Tobacco in Nicaragua.

In April of 2012, tobacco (Nicotiana tabacum) plants with symptoms resembling those caused by viral infection were observed in commercial fields in several departments in Nicaragua, including Esteli and Nueva Segovia. Heavy infestations of the psyllid Bactericera cockerelli, a major insect pest of potato and other solanaceous crops and vector of the bacterium "Candidatus Liberibacter solanacearum" (Lso) (2,3), were observed in the affected fields. All cultivars grown were affected and 5 to 100% of plants in each field were symptomatic. Symptoms on affected plants included apical leaf curling and stunting, overall chlorosis and plant stunting, young plant deformation with cabbage-like leaves, wilting, internal vascular necrosis of stems and leaf petioles, and overall poor leaf quality. Plant samples were collected from a total of three psyllid-infested fields in the municipalities of Esteli, Condega, and Jalapa (one field/municipality). The plant samples were first tested for viruses, including Potato virus Y, Tobacco mosaic virus, Cucumber mosaic virus, and Impatiens necrotic spot virus, using Immunostrips (Agdia, Elkhart, IN) and no virus was detected. Total DNA was extracted from leaf tissues of a total of 22 plants, including 17 symptomatic plants and five asymptomatic plants from two cultivars (Corojo and Habano) with the cetyltrimethylammonium bromide (CTAB) buffer extraction method (2,4). The DNA samples were tested by PCR using specific primer pairs OA2/OI2c and OMB 1482f/2086r, to amplify a portion of 16S rDNA and the outer membrane protein (OMB) genes, respectively, of Lso (2). 16 rDNA and OMB gene-derived fragments of 1,168 and 605 bp, respectively, were amplified from the DNA of 13 of 17 (76.5%) symptomatic plants, indicating the presence of Lso. No Lso was detected in the five asymptomatic plants. DNA amplicons of three plant samples (one plant/field) with each primer pair were cloned and two to four clones of each of the six amplicons were sequenced. BLASTn analysis of the 16S rDNA consensus sequences was the same for all three locations (GenBank Accession Nos. KC768323, KC768324, and KC768325) and showed 100% identity to numerous 16 rDNA sequences of Lso in GenBank, including accessions HM245242, JF811596, and JX559779. Similarly, identical OMB consensus sequences were observed in all three locations (KC768331 and KC768332 for Jalapa and Condega, respectively) that are 97 to 100% identical to a number of Lso sequences in GenBank (e.g., CP002371, FJ914617, JN848754, and JN848752). A second OMB sequence was isolated from the Esteli sample (KC768333) that was 98% identical with the consensus sequences from this and other sites and 100% identical to an OMB sequence from Lso in GenBank (JN848754). To our knowledge, this is the first report of Lso associated with tobacco. Tobacco is an important crop in many parts of the world, including Central and South America. This bacterium has also caused millions of dollars in losses to potato and several other solanaceous crops in the Americas and New Zealand (3). In addition, this plant pathogen has been reported as serious pest of carrot in Europe, where it is associated with the psyllids Trioza apicalis and B. trigonica (1,4).

High Mortality Rate due to False Gid in a Sheep Herd

The sheep nasal bot, Oestrus ovis (Diptera: Oestridae), is a cosmopolitan parasite commonly found in sheep and occasionally goats. Rarely a bot will migrate into the sheep brain (false gid). Following the complaint of an animal husbandman about high mortality rate in a sheep herd, the herd was clinically, hematologically, and pathologically examined exactly. Clinical, hematological, and pathological findings were described in the text. Necropsy findings showed heavy infestation with Oestrus ovis larvae. The herd was treated with Ivermectin. After treatment all patients without nervous sings were recovered. Patients with nervous signs did not respond to treatment, but new cases of disease did not occur and the mortality stopped. In the present report, a high mortality rate due to false gid in a sheep herd was described. The prevention and control of the disease are important because of economic losses and the possibility of transmission to the human.

Detection of Cysticercus bovis in abattoirs in Northern Ireland between 2001 and 2011

Cysticercus bovis is the larval stage of the human beef tapeworm, Taenia saginata . Cattle act as the intermediate host for the parasite. Cattle become infected through access to environments...

Occurrence of phthirapteran ectoparasite parasitizing on domestic dogs, Canis familiaris (Linne) in Jaunpur district (U.P.)

Only two species of biting louse, Heterodoxus spiniger (Enderlein) (43.27%) and Trichodectes canis (De Geer) (13.47%) have been recorded from 245 dogs examined in twelve different localities in Jaunpur district during 2009 to 2011. Female dogs were found most prevalent in comparison to the male in both the cases. Different parameters like host sex, hair colour, hygienic condition, health and age group have been taken into the consideration during the survey. Older age groups, unhealthy and unhygienic condition of dogs were found more prone for the lice. In case of relative intensity of lice 38% were found moderately infested with H. spiniger while 23% and 16% were very light and light infested. Only 12% could be recorded heavy infested dog while 8% remained very heavy infested. Similar trends were recorded for T. canis where moderate infestation followed by very light, light, heavy and very heavy infestation respectively. Besides these two lice, some other ectoparasites (ticks, mites and fleas) (5.31%) were also recorded casually but not used the data for prevalence.

Arsenic toxicosis in sheep: The first report from Iran

Arsenic contamination of groundwater has been previously reported in Ghopuz, a village located in the Northwest of Iran. Samples were taken from consuming and irrigation water and plants of the region for chemical analysis. A seven-year old ewe, which had lived in and fed a lifelong at the same place, with clinical signs such as weakness, wasting and inappropriate integument was necropsied. Grossly, buccal erosion, stomatitis, cutaneous ulcers and serous atrophy of fat deposits were observed. Rumen contents, wool and several tissue samples were obtained for toxicological and histopathological examinations. Mean arsenic concentration in the spring water, irrigation water and grass/algae were 70.11, 48.74 and 141.85 ppb (µg/kg), respectively. Arsenic levels were 486.73, 247.94, 127.92, 125.97 and 231.24 ppb in wool, skin, rumen contents, liver and kidney, respectively. Microscopic study revealed hyperemia and heavy parasitic infestation of the abomasal wall. Hyperemia and regeneration of renal tubule epithelia were observed in kidneys and hyperkeratosis, suppurative deep dermatitis and paniculitis were found in skin. Periacinar fibrosis and a poorly differentiated cholangiocarcinoma were seen in liver. In pancreas, reduced cell density of islands of Langerhans was noticeable. In the central nervous system, perineuronal and perivascular edema, ischemic changes in gray matter neurons, and microcavitation of white matter were present. Our findings confirmed chronic arsenic toxicosis in small ruminants in this region. It can be concluded that long-term consumption of arsenic contamined water and forage may be associated with chronic arsenic poisoning in domestic animals and human beings, with consequent neoplastic disease and induction of diabetes in this region.

A Mealybug Phenacoccus multicerarii Granara de Willink (Hemiptera: Pseudococcidae)

Florida Department of Agriculture and Consumer Services, Division of Plant Industry inspector Lisa Hassell collected the mealybug Phenacoccus multicerarii, a new continental record, from a retail nursery in Fernandina Beach (Nassau County, on 15 June 2011). On a return visit to the store, the mealybug was observed in high numbers on numerous host plants. Three days later, an additional find was made at a nursery in Apopka that had exchanged plant material with the nursery in Jacksonville. No further infestations were reported until October 2012, when a heavy infestation was discovered on a residential planting of coleus in Cooper City (Broward County). This mealybug was described in 2007 from a sample collected in Caracas, Venezuela, in 1949 from an unknown host (Granara de Willink and Szumik 2007). This 2-page fact sheet was written by Ian Stocks, and published by the UF Department of Entomology and Nematology, April 2013.&#x0D; http://edis.ifas.ufl.edu/in993

Paratrichodina africana (Ciliophora): A pathogenic gill parasite in farmed Nile tilapia

Trichodinids are ciliated protozoa that are widely known as one of the main groups of fish parasites. The genus Trichodina presents the greatest species diversity. However, records of Paratrichodina species are scarce, and little is known about their pathogenicity in hosts. The present study provides new records of Paratrichodina africanaKazubski and El-Tantawy (1986) in Nile tilapia from South America and descriptions of pathological changes and seasonality. A total of 304 farmed fish were examined. From gill scraping, parasites were identified using Klein's nitrate impregnation method. Gill samples were fixed for histopathological analysis. Small trichodinid found in this study have a prominent blade apophysis and narrow central part and blade shape that corresponds to the characteristics of P. africanaKazubski and El-Tantawy (1986). Gill lesions were proportional to parasite intensity, in which the gill tissue was compromised in heavy infestation. Proliferative disturbances were found, including epithelial hyperplasia, desquamation, and mononuclear and eosinophilic infiltrate that culminated in necrosis. We did not observe a seasonality effect on the occurrence of P. africana. This ciliated protozoan causes compromised respiratory capacity that leads to severe gill lesions and currently is an important pathogen that afflicts intensive tilapia cultures in Brazil.

Elimination of Iron Deficiency Anemia and Soil Transmitted Helminth Infection: Evidence from a Fifty-four Month Iron-Folic Acid and De-worming Program

BackgroundIntermittent iron-folic acid supplementation and regular de-worming are effective initiatives to reduce anemia, iron deficiency, iron deficiency anemia, and soil transmitted helminth infections in women of reproductive age. However, few studies have assessed the long-term effectiveness of population-based interventions delivered in resource-constrained settings.Methodology/Principal FindingsThe objectives were to evaluate the impact of weekly iron-folic acid supplementation and de-worming on mean hemoglobin and the prevalence of anaemia, iron deficiency, and soil transmitted helminth infection in a rural population of women in northern Vietnam and to identify predictive factors for hematological outcomes. A prospective cohort design was used to evaluate a population-based supplementation and deworming program over 54 months. The 389 participants were enrolled just prior to commencement of the intervention. After 54 months 76% (95% CI [68%, 84%]) were taking the iron-folic acid supplement and 95% (95% CI [93%, 98%]) had taken the most recently distributed deworming treatment. Mean hemoglobin rose from 122 g/L (95% CI [120, 124]) to 131 g/L (95% CI [128, 134]) and anemia prevalence fell from 38% (95% CI [31%, 45%]) to 18% (95% CI [12%, 23%]); however, results differed significantly between ethnic groups. Iron deficiency fell from 23% (95% CI [17%, 29%]) to 8% (95% CI [4%, 12%]), while the prevalence of iron deficiency anemia was reduced to 4% (95% CI [1%, 7%]). The prevalence of hookworm infection was reduced from 76% (95% CI [68%, 83%]) to 11% (95% CI [5%, 18%]). The level of moderate or heavy infestation of any soil-transmitted helminth was reduced to less than 1%.Conclusions/SignificancePopulation-based interventions can efficiently and effectively reduce anemia and practically eliminate iron deficiency anemia and moderate to heavy soil transmitted helminth infections, maintaining them below the level of public health concern.