PurposeStressors due to the workload in the ambulance service are numerous and can be positively counteracted by work-related behaviors and experiences. We analyzed the subjective perceptions of workload and stress as a function of work-related behavior and experience patterns among emergency service personnel (EMP).MethodsA total of 276 EMP (94.6% men) participated (average age: 39.3 ± 8.04 years). Data on the stress situations of ambulance service staff according to the Slesina questionnaire, the Recovery–Stress Questionnaire (EBF), and the Questionnaire for Physical, Psychological and Social Symptoms (KOEPS) were obtained. Participants were classified into four patterns (A, B, G, and S) based on the Work-Related Behavior and Experience Patterns (AVEM) questionnaire.ResultsOverall, 32% of EMP were classified into AVEM risk patterns A and B. For half of the stress factors examined (23/46), the data were significantly different among the AVEM groups. Individuals with AVEM risk patterns have higher stress and lower recovery scores on the EBF and more physical, psychological, and social-communicative impairments shown using the KOEPS (all variables p < 0.001). Analyses showed moderate correlations among the AVEM dimensions (exceptions included striving for perfection, subjective importance of work, and work-related ambition), and the main scales of the EBF and KOEPS.ConclusionWork-related patterns of behavior and experience are used to determine how stress is handled, and it is possible to distinguish between patterns that promote health and those that hazardous to it. Individuals with AVEM patterns that are a risk to their health experience high stress, low recovery, and increased physical, psychological, and social-communicative impairments. Health-promoting work-related behaviors can be used to counteract stress. Companies developing preventive health promotion measures should focus on individuals with AVEM patterns that are a risk to their health.Supplementary InformationThe online version of this article (10.1007/s10049-022-01076-y) contains further tables and figures. The article and additional material are available at www.springermedizin.de. Please enter the title of the article in the search field. You will find the additional material under “Supplementary Information” at the article.