Lonicera Caerulea Research Articles

Совершенствование технологии размножения жимолости синей (Lonicera caer ulea L.) одревесневшими черенками

Sweet-berry honeysuckle (Lonicera caerulea L) is a promising berry crop that can be marketed throughout the summer due to the diversity of cultivars with different ripening periods. The area occupied by honeysuckle in Russia is 700 hectares. To increase industrial plantations, it is necessary to provide farms with high-quality planting material. One of the ways of obtaining seedlings is through wood cuttings. The aim of our work is to select a root formation stimulator for woody honeysuckle cuttings. The experiment was carried out in the Moscow region. The honeysuckle cultivars “Volkhova”, “Nimfa” and “Pavlovskaya” and the preparations “Kornevin” and “EcoFus” were used as research objects. The experience is presented in four variants, with 100 cuttings in each variant. Two-factor analysis of variance showed no influence of cultivar characteristics on the rooting of cuttings, root volume, the number and length of shoots in seedlings. The rooting percentage of the cultivars varied from 73 to 81%, root volume (4.12–4.64 ml), number (2.63–2.81 pcs) and shoot length (4.32–6.16 cm) also differed insignificantly. A significant effect of the growth regulators on the traits studied was observed. The best development of the above-ground and underground parts of seedlings was observed with the combined application of the preparations “Kornevin” and “EcoFus”, and the rooting ability was also at a high level (81%), which indicates the feasibility of using the studied preparations. According to the results of other studies on such crops as balsam, gloxinia and cyclamen, the effectiveness of using the “EcoFus’ preparation has been proven. The number of leaves increased by 35–40% depending on the plant species. Studies on the combined use of the preparations “Zircon”, “Siliplant” and “EcoFus” showed a positive effect on the vegetative reproduction and productivity of wild garlic. The preparations “Siliplant”, “EcoFus”, and “Cytovit” showed high efficiency on the yield of the grape cultivars “Ranniy Magaracha” and “Karaburnu”. The rooting of forsythia cuttings with “EcoFus” showed negative results. In this experiment, the multiplication factor could be affected by the concentration of the preparation and the duration of exposure, as the green cuttings have unripened tissues, that could have a negative effect. It is also necessary to take into accou nt the characteristics of the culture itself. Further research is needed in this area.

Market potential and value-added opportunities of cold-hardy berries and small fruits in the Intermountain West, USA.

Novel cold-hardy berries and small fruits represent an opportunity for growers in the Intermountain West, USA, as the harsh environment is not suited for other common berries and small fruits. This study analyzed the fresh market and value-added potential of haskap berries (Lonicera caerulea), saskatoon berries (Amelenchier alnifolia), and dwarf sour cherry (DSC) fruit (Prunus x kerrasis) by instrumental and consumer studies. Fresh and 2-week stored haskap (cv. Aurora), saskatoon (Lee 3), and DSC (Romeo) were measured for fruit weight, flesh firmness, bulk titratable acidity, pH, and soluble solid content. Participants (n=115) in at-home sensory tests scored these fruits for overall liking (OL, 9-point hedonic scale), purchase intent (PI, 5-point scale), and willingness-to-pay (WTP, 5-point scale). Ten participants further shared insight on these fruits in focus groups. Instrumental testing found a significant decrease in flesh firmness for 2-week stored haskap, but the consumers' OL was still comparable to fresh haskap. The fresh and 2-week stored haskap received significantly higher OL, PI, and WTP scores (7.7±1.0, 3.8±1.0, and $3.7±1.0; 7.7±1.2, 3.8±1.1, and $3.7±1.0, respectively) compared to saskatoon (6.1±1.8, 2.8±1.1, and $3.0±0.9, respectively) and DSC (5.6±2.2, 2.5±1.2, and $3.1±1.0, respectively) (α=0.05). The focus groups indicated that participants want to support local produce. The participants expressed interest in fresh unprocessed haskap berries, but preferred saskatoon and DSC in different value-added formats. Hence, this study concluded that there is an untapped fresh-market potential for haskap berries and there are value-added opportunities to extend the sale season and improve the palatability of saskatoon and DSC. PRACTICAL APPLICATION: This research has examined consumer perception of three species of novel small fruits by determining fresh-market potential and linking this to the opportunity for value-added product development. For haskap berries, the study not only indicated fresh market potential, but for the cultivar Aurora, consumer liking was not meaningfully altered by 2 weeks of cold storage. These results are meaningful because they will assist growers in the Intermountain West with market planning, including the possibility of formulating products that utilize these novel crops. This study provides growers the opportunity to diversify their income stream by utilizing local produce.

ANTIOXIDANT ACTIVITY AND PHENOLIC PROFILE OF FRUITS FROM SOME ROMANIAN SHRUB FRUIT VARIETIES

The berries are rich in polyphenols, vitamins, and other bioactive compounds, and exhibit antioxidant, antimicrobial, anti-inflammatory and anticancer activities. In this work, antioxidant activity (AA) and a phenolic profile of berries fruits belonging to the Aronia melanocarpa (Michx.) Elliott, ʹMelromʹ cv., Lonicera caerulea var. kamtschatica, ʹKamiʹ cv., and Sambucus nigra L., ʹElromʹ cv. were studied. All three cvs. were previously patented by the Research Institute for Fruit Growing Pitesti, Romania. Alcoholic extracts from berries were obtained by four methods: continuous, maceration, ultrasounds and microwaves-assisted. The antioxidant content of extracts was evaluated by an HPLC method. AA was determined by DPPH free radical scavenging method. Total phenol, flavonoid, and anthocyanin contents were determined by spectrophotometric methods. The AA values were reported as equivalents (µg mL-1 extract) caffeic and gallic acids, morin, quercetin, rutin, and are in concordance with the results of the chromatographic method. In addition, the chromatographic method allowed identifying the p-coumaric acid, caffeic acid, chlorogenic acid, rutin, and epicatechin. The experimental results have shown that analyzed fruit extracts have high antioxidant activity, due mainly to flavonoids and anthocyanins in high concentration. Therefore, extracts from mentioned berries can be used in different nutraceutical products with high antioxidant potential.

MACRO- AND TRACE ELEMENTS COMPOSITION OF THE BLUE HONEYSUCKLE AND ELM-LEAF SPIREA CE-NOPOPULATIONS IN THE GEOCHEMICALLY ABNORMAL ENVIRONMENT IN THE MOUNTAIN ALTAI

The specificity of macro- and trace elements accumulation in the leaves and stems of Lonicera caerulea and Spiraea chamaedryfolia cenopopulations growing at the sites with serpentinite inclusions in the Mountain Altai (Russia) was studied. The total content of macro- and trace elements in plants and soil was determined by atomic emission spectrometry, and the mobile forms, extracted by the ammonium acetate solution with pH 4.8, were measured by atomic absorption. The study revealed species-specific accumulation of some chemical elements in plant organs. The elm-leaf spirea plants were found to accumulate Mn and Ni more intensively in comparison with the blue honeysuckle plants, and to concentrate such trace elements as Zn, Cu and Mn, whereas the blue honeysuckle plants concentrated Zn and Cu. Species-specific statistically significant correlations between mobile Ni, Cd, Cr, Pb and Mg soil and phytomass content were also revealed. Negative correlation was found between the total P and Cr content in soil and plants, whereas total Fe, Cd and Ni in soil and plants correlated positively. The leaves of Lonicera caerulea and Spiraea chamaedryfolia, growing at the site with ultra-basic rock outcroppings, had Ni concentrations exceeding the maximum permissible level and decreased Ca concentration.

Extraction of anthocyanins from haskap using cold plasma-assisted enzyme.

Haskap berries (Lonicera caerulea L.) are rich in anthocyanins. Cold plasma-assisted enzyme method (CPEM) is an innovative method for green extraction of anthocyanins, which was optimized by an artificial neural network-genetic algorithm (ANN-GA) to maximize the yield. In this study, seven factors were screened using by Plackett-Burman design based on single-factor experiments and optimized by ANN-GA. The results showed that the maximum total anthocyanin content (TAC, 42.45 ± 0.25 g cyanidin-3-glucoside equivalent (C3G)kg-1 dry weight, DW) was obtained under optimal pretreatment power of 192 W, pretreatment time of 29 s and liquid-to-solid ratio of 39 mL g-1 . Cleavage and porosity appeared on the surface of the treated sample. The active ingredients and antioxidant capacity of the CPEM extracts were identified by ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Compared with other extraction technologies, CPEM presents the advantages of shortening the extraction time, reducing the solvent volume, and significantly increasing active ingredients and antioxidant activity. The ANN-GA has better predictive and higher accuracy than the response surface methodology (RSM) model and is more suitable for optimizing the CPEM by greatly improving the process yield and the utilization of biomass, thus contributing to the sustainability of the agri-food chain. © 2022 Society of Chemical Industry.

Variation of Fruits Morphometric Parameters and Iridoid Content of Lonicera caerulea L. Germplasm Collection

The dark-blue, fleshy berries of Lonicera caerulea L. are used as fresh or processed in many products such as juice, smoothie, wine, compote, jam, marmalade, candies, chocolate, cake, or extracts. L. caerulea fruits are appreciated in the food industry and medicine mainly due to a high content of biologically active phenolic compounds, especially anthocyanins and vitamin C – in the amount up to 186 mg.100 g-1, and health-promoting properties. Thirty-four genotypes of Lonicera caerulea species originated from germplasm collection of the Forest-Steppe of Ukraine in M.M. Gryshko National Botanical Garden of NAS of Ukraine (NBG) (Kyiv, Ukraine) and were characterized by using the morphometric traits and iridoids content. Differences between the genotypes were significant in all observed parameters. The fruits of L. caerulea collection varied in evaluated morphometric parameters as follows: fruit weight 0.70–1.54 g, length 15.33–26.52 mm, diameter 7.12–13.10 mm. The shape indexes of fruits varied from 1.42 to 3.65. Our results showed that the content of iridoids ranged from 97 to 314 mg.100 g-1 of fresh L. caerulea fruits. Interestingly, L. caerulea berries taste differs between the varieties, those with bitter or sour-bitter taste are distinguished by the highest content of iridoids (225–314 mg.100 g-1), while berries without bitter taste were characterized by significantly lower contents. Thus, honeysuckle berries should be selected preferentially regarding their bitterness, due to the anti-inflammatory and antioxidative properties of iridoids.

Metabolomic prof iles of Ribes nigrum L. and Lonicera caerulea L. from the collection of the N.I. Vavilov Institute in the setting of Northwest Russia.

Recently, the trend of using fruit and berry crops as ingredients for functional and dietary nutrition, the development and implementation of f lavors, pigments, new medicines and dietary supplements has been actualized. Because the direction of use depends on the biochemical properties of fruits, which are determined not only by species and varietal characteristics, but also by reproduction conditions, the study of the biochemical composition of fruits grown in various regions of the world continues to be relevant. In this regard, the collection of N.I. Vavilov Institute (VIR), which has a wide diversity of fruit and berry crops, is of great interest for study. Ribes nigrum fruits have a balanced set of sugars, organic acids, essential oils, microelements, a high content of vitamins, anthocyanins, pectins. Lonicera caerulea fruits are characterized by high values of phenolic substances: biof lavonoids, hydroxycinnamic acids, f lavonols, polyphenols, anthocyanins, as well as vitamins, carotenoids, iridoid glycosides and other natural antioxidants. The investigation of L. caerulea and R. nigrum fruit's accessions from the VIR collection using gas-liquid chromatography with mass spectrometry allows us to obtain new information about the biochemical characteristics of fruits, to identify L. caerulea and R. nigrum varieties with optimal economically valuable characteristics, to determine the specif icity of L. caerulea and R. nigrum metabolomic spectra in the setting of Northwest Russia. As a result of the analysis, typical compounds of the metabolomic prof ile of each culture were identif ied. Organic acids, phenol-containing compounds and polyols prevailed in L. caerulea, while mono- and oligosaccharides, in R. nigrum. The qualitative composition of the black currant varieties 'Malen'kii Printz', 'Dobriyi Dzhinn', 'Tisel', 'Orlovskii Val's', and blue honeysuckle 'S 322-4', 'Malvina', 'Leningradsky Velikan' was optimal for food consumption; the varieties of blue honeysuckle 'Bazhovskaya' and black currant 'Aleander' had a good representation of biologically active compounds, which makes samples attractive as raw materials for the production of biologically active additives, including with the use of microorganisms' cultures.

Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota

ScopeHigh-fat diet (HFD) induces imbalance in the small intestine environment, where fat digestion and absorption mainly take place. This study aimed to elucidate the mechanisms by which Lonicera caerulea polyphenols (LCP) might inhibit fat absorption, from the perspective of small intestine microbiota and epithelial barrier integrity. Methods and resultsMale Sprague-Dawley rats were given HFD with or without co-administration of LCP for 8 weeks. The results showed that LCP supplementation significantly decreased the levels of serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C), and increased the contents of fecal sterols, in HFD rats. LCP also inhibited the dysfunction of the small intestine epithelial barrier, via alleviating the oxidative stress activated by Nrf2-ARE pathway, and by modulating the expressions of pro-inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), nuclear factor kappa-B p65 (NF-κB p65) and inducible nitric oxide synthase (iNOS) in the small intestine. Additionally, LCP administration restored the balance in small intestine microbiota and increased the abundance of the specific bacteria, such as Lactobacillus, involved in fat absorption. ConclusionOur results demonstrated that LCP may be beneficial to inhibit fat absorption. The mechanism seems to be associated with the protection of the epithelial barrier integrity and the modulation of specific bacteria in the small intestine.

Preparation, characterization and bioactivities of selenized polysaccharides from Lonicera caerulea L. fruits

Native polysaccharide was obtained from Lonicera caerulea L. fruits (PLP). Two selenized polysaccharides (PSLP-1 and PSLP-2) were synthesized by the microwave-assisted HNO3-Na2SeO3 method, where the selenium (Se) contents were 228 ± 24 and 353 ± 36 μg/g, respectively. The molecular weights of PLP, PSLP-1, and PSLP-2 were 5.9 × 104, 5.6 × 104, and 5.1 × 104 kDa, respectively. PSLP-1 and PSLP-2 contained the same type of monosaccharides as PLP but with different molar ratios. The main chain structure of the native polysaccharide was not changed after selenization. PLP, PSLP-1, and PSLP-2 contained the same six types of glycosidic bonds. Bioactivity assays revealed that the two selenized polysaccharides possessed better antioxidant activities than PLP, but their bile acid-binding abilities and inhibitory activities on acetylcholinesterase (AChE) had weakened. In summary, PLP, PSLP-1, and PSLP-2 may be promising Se supplements in functional foods and inhibitors for the treatment of AChE.

First report of leaf spot disease caused by Alternaria alternata on Lonicera caerulea L. in China.

Relatively few studies investigating plant diseases in blue honeysuckle (Lonicera caerulea L.) have been conducted in China. In September 2021, a leaf spot disease was observed on approximately 10% of blue honeysuckle 'Lanjingling' leaves in a 0.02 ha field plantation in Tiekuang Street (124.36°E, 40.12°N) in Dandong City, Liaoning Province, China. The main symptom consisted of leaf spots with black mildew centers typically surrounded by yellow halos. Small pieces (3-4 mm) of the infected leaves were plated onto potato dextrose agar (PDA) medium as described by Wang et al. (2020) and six purified cultures were obtained through single spore culture on PDA. The observed conidia, consistent with the morphology of Alternaria alternata, were obpyriform and dark brown, measuring 5.8 to 15.3 μm × 7.9 to 42.5 μm, with 1-6 transverse septa and 0-3 longitudinal septa (n = 50) (Simmons 2007). For molecular verification, genomic DNA was extracted from a representative isolate LD-8. The ITS (GenBank OL454815), GPD (GenBank OL601993), TEF (GenBank OL538256), RPB2 (GenBank OL601966), and Alt (GenBank OL538257) genes were partially amplified with the respective primers ITS1/ITS4 (White et al. 1990), GPD1/GPD2 (Woudenberg et al. 2015), EFl-728F/EFI-986R (Carbone and Kohn 1999), RPB2-5F2/RPB2-7CR (Liu et al. 1999), and Alt-for/Alt-rev (Hong et al. 2005). BLAST analysis revealed that these genes shared 99%-100% identity with OK345332, MK451977, MN756011, KU933459, and MN655781, respectively. A greenhouse experiment was conducted using six, healthy two-year-old blue honeysuckle 'Lanjingling' plants to observe disease development (Mirzwa-Mróz et al. 2018). After 10 d, we noted typical leaf spot symptoms on inoculated leaves sprayed with a conidial suspension (106 spores/mL) while no symptoms were detected on uninoculated leaves. The same isolate, reisolated from infected leaves with the same morphological and molecular traits, was also identified as A. alternata, confirming Koch's postulates. The fungus was previously reported in cockscomb plants in Heilongjiang Province, China (Wang et al. 2020). To our knowledge, this is the first report of leaf spot disease caused by A. alternata in blue honeysuckle grown in China. This study will provide a basis for future development of effective protection strategies against blue honeysuckle leaf spot in China.

Blue honeysuckle rich in cyanidin-3-O-glucoside inhibited adipogenic differentiation by modulation of the adipogenesis pathway in 3T3-L1 adipocytes

Background: Blue honeysuckle (BH; Lonicera caerulea L.), which is a traditional medicinal plant, is known to be a rich source of anthocyanins and phenolic acids due to its strong antioxidant and anti-inflammatory activities. Its anti-obesity effects, which are a result of attenuating abnormal lipid and glucose metabolisms, have also been reported.
 Aim: The purpose of this study is to investigate the effect of BH on genes and proteins that are involved in the adipocyte differentiation using 3T3-L1 cells.
 Methods: The effects of the water extracts of the BH were examined on adipogenesis and lipolysis using a biochemical and molecular analysis of the 3T3-L1 cells.
 Results: Cyanidin-3-O-glucoside (C3G) from the BH extract was determined in order to contain 1.67 mg/g by the high-performance liquid chromatography analysis. The lipid accumulation in the adipocytes was reduced, which ranged from 58 to 26% in the BH (500 and 1,000 µg/mL) compared to the control group. The lipolysis that was measured by the glycerol content was not affected by the BH at 1,000 μg/mL. The BH downregulated the expression of the main transcription factors of the adipogenesis pathway, such as peroxisome proliferator-activated receptor γ, 1, 2, adipose differentiation-related protein, CCAAT/enhancer binding protein α, and acetyl CoA carboxylase, while increasing the expression of the Adenosine monophosphate (AMP)-activated protein kinase α.
 Conclusion: These findings suggest that the BH is a good source of C3G, and it could be effective in regard to inhibiting the adipogenesis as opposed to the lipolysis, which indicates the potential for natural anti-obesity ingredients.

Results of the study of blue honeysuckle (<i>Lonicera Caerulea</i> L.) in Federal Horticultural Center for Breeding, Agrotechnology and Nursery

The results of a long-term study of varieties and samples of blue honeysuckle Lonicera caerulea L. are presented in the conditions of the Moscow region. The purpose of the work is to identify sources of economically valuable traits. 56 domestic samples of various origins were studied. The most valuable forms for cultivation and further breeding work have been identified, which have large fruit, good fruit taste, lack of tendency to untimely awakening of the kidneys and shedding of fruits during ripening. These characteristics distinguish the varieties of medium-early and medium maturation - Lebedushka, Marina, Kuminovka, Lazurnaya, Moskovskaya 23, Gzhelskaya Early, medium-late and late maturation - Leningrad Giant, Julia, Violet. The Nizhny Novgorod Early variety and the Tatiana sample are the earliest, they are also sources of good fruit taste and are not prone to bud awakening in autumn, but their fruits crumble heavily when ripe.

Assessment of the size and shape of berries using the ImageJ program on the example of honeysuckle

Background. Digital technologies are increasingly used in agriculture to solve a variety of problems. However, in horticulture and industrial production of fruit and berry crops, qualitative evaluation and scoring of the most important morphological indicators of fruits are still common, and measurements are carried out manually. The aim of this study was to develop an algorithm for using the ImageJ package for quick and accurate measurements of the size and shape of berries.Materials and methods. The material included 190 berries of 3 blue honeysuckle (Lonicera caerulea L.) cultivars: ‘Amazonka’, ‘Lazurit’, and ‘Lenita’. The berries were laid out on a white sheet of paper with a ruler on top of the glass and photographed with additional lighting from below. The analysis of the obtained images was carried out using the public domain package ImageJ (v. 1.51k) and included automatic search for objects and their measurement by 7 indicators: area, perimeter, maximum and minimum Feret diameters, aspect ratio, circularity, and surface roundness (1-Solidity). Statistical analysis included the calculation of the minimum, maximum and mean values with a nonparametric 95% CI (bootstrap, percentile method), comparison of cultivars using the Kruskal–Wallis test, and search for the most typical objects based on the results of a between-group PCA.Results. It was shown how the size and shape indicators from the ImageJ package related to classical measurements in pomology, including length, diameter, and berry shape index. For all indicators, the differences between cultivars were highly statistically significant (p < 0.001). The prospects of using the surface roughness index for quantitative characterization of the degree of deviation of fruits from their natural shape due to mechanical and other deformations are discussed.Conclusion. The results of the automatic image analysis in the ImageJ package can be used in horticulture, breeding, and production of fruits and berries.

Novel production of β-cryptoxanthin in haskap (Lonicera caerulea subsp. edulis) hybrids: Improvement of carotenoid biosynthesis by interspecific hybridization

Carotenoids are important color pigments that contribute to human health. Haskap (Lonicera caerulea subsp. edulis), a shrub mainly distributed in Hokkaido, produces violet-blue berries low in carotenoid amount. We have conducted interspecific hybridization between Miyama-uguisukagura (Lonicera gracilipes var. glandulosa) and haskap to obtain interspecific hybrids for improving fruit quality. In this study, we compared four carotenoid concentrations in fruits using liquid chromatography/tandem mass spectrometry (LC/MS/MS) to reveal carotenoid accumulation changes by interspecific hybridization. β-Carotene was the main carotenoid, and the concentration in interspecific hybrids (0.49–0.77 mg/100 g FW) was higher than Miyama-uguisukagura (0.37 mg/100 g FW) and haskap (0.25–0.35 mg/100 g FW). β-Cryptoxanthin concentration was below the quantification limit in haskap strains; however, we could quantify β-cryptoxanthin in interspecific hybrids. β-Cryptoxanthin concentration in Miyama-uguisukagura fruits and the interspecific hybrid strain that contained higher β-cryptoxanthin tended to increase during maturation, although others did not increase. Our study revealed that interspecific hybridization changed carotenoid biosynthesis, increased β-carotene, and induced the conversion of β-cryptoxanthin to haskap. This study proposes a strategy to expand color variation by interspecific hybridization in fruit breeding programs and provides novel materials to analyze the “vigor” phenomenon in hybrid plants.

The Effective Analysis for Blue Honeysuckle Extract in the Treatment of Hepatocellular Carcinoma.

To further determine how BHE affected the growth of HCC cells, the proportion of each cell cycle phase was explored in HCC cells by flow cytometry. Blue honeysuckle (Lonicera caerulea L.) is a species of bush that grows in eastern Russia. Blue honeysuckle extract (BHE) is rich in bioactive phytochemicals which can inhibit the proliferation of tumor cells. The mechanism underlying the anticancer activity of BHE in primary liver cancer is poorly understood. The purpose of this study was to evaluate the growth inhibition mechanism of bioactive substances from blue honeysuckle on hepatocellular carcinoma (HCC) cells and to explore its protein and gene targets. The compounds in BHE were determined by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Cell counting kit-8 (CCK8) assay was used to evaluate the effects of BHE on HCC cell proliferation, and flow cytometry assay (FCA) was used to determine how BHE arrested the proportion of each cell cycle phase in HCC cells. Western blot (WB) was performed to determine the expression of cell cycle-related proteins in HCC cells treated with different concentrations of BHE. The xenograft tumor animal models were established by HCC cell implantation. The results showed that cyanidin-3-o-glucoside and cyanidin-3-o-sophoroside which are the main biologically active components were detected in BHE. BHE is highly effective in inhibiting the proliferation of HCC cells by arresting the HCC cell cycle in the G2/M phase. BHE also downregulated the expression of conventional or classical dendritic cells-2 (cDC2) and cyclin B1 by promoting the expression of myelin transcription factor 1 (MyT1) in HCC cells. The weight and volume of xenografts were significantly decreased in the BHE treated groups when compared to the control group. BHE increased the expression of MyT1 in xenograft tissues. These findings showed that blue honeysuckle extract inhibits proliferation in vivo and in vitro by downregulating the expression of cDC2 and cyclin B1 and upregulating the expression of MyT1 in HCC cells.

BIOCHEMICAL COMPOSITION OF FRUITS OF LONICERA CAERULEA L. AND ITS SUBSPECIES DURING IN-TRODUCTION IN THE CONDITIONS OF THE BASHKIR URALS

Lonicera caerulea L. and its subspecies Lonicera caerulea subsp. edulis (Turcz. ex Freyn) Hulten and Lonicera caerulea subsp. altaica Pall. belong to the genus Lonicera L. family Caprifoliaceae Juss., they are valuable fruit plants that are used in medicine, food industry, agriculture. The value of these species lies in the early ripening of fruits and a high content of vitamin C and other biologically active compounds.
 The aim of the study was the qualitative and quantitative determination of the main groups of biologically active substances – organic acids and tannins, in the fruits of three species of the genus Lonicera from the collection of the South Ural Botanical Garden-Institute and the selection of the most promising species. For analysis, honeysuckle fruits were collected in the full ripening phase and dried to an air-dry state. Phytochemical studies were carried out according to generally accepted methods.
 As a result of the study, it was found that all the studied honeysuckle samples contain citric, malic, succinic, oxalic, ascorbic acids, and tartaric acid in Lonicera caerulea subsp. edulis. According to the quantitative content of ascorbic acid and the sum of organic acids, a species is distinguished in Lonicera caerulea subsp. edulis, whose fruits contain 174 and 1723 mg/100 g respectively, and in Lonicera caerulea subsp. altaica and Lonicera caerulea their content is below. The analysis of fruits for the content of tannins showed, that they are dominated by substances of condensed nature, which are based on catechism, and in a larger amount they accumulate in Lonicera caerulea – 426 mg/100 g in a smaller amount – in Lonicera caerulea subsp. edulis – 171 mg/100 g.
 The obtained data allow us to recommend the studied species of the genus Lonicera as a promising source of raw materials for the creation of medicinal plant products based on them, enriched with vitamins and other valuable biologically active substances on their basis.

Component characteristics and reactive oxygen species scavenging activity of anthocyanins from fruits of Lonicera caerulea L.

In this study, we qualitatively and quantitatively characterized anthocyanins from 61 different Lonicera caerulea L. genotypes harvested from northeast China and measured their reactive oxygen species (ROS) scavenging activity. Among the anthocyanin extracts obtained, nine anthocyanins were identified, and the anthocyanin compositions could be divided into three classes and seven subtypes according to their numbers and distribution. Furthermore, their total content by fresh weight was ranged from 158.44 mg/100 g to 1751.44 mg/100 g across all genotypes; their hydroxyl radical scavenging activity and superoxide anion scavenging activity ranged from 13.3 %–99.9 % and 21.5 %–82.8 %, respectively. We also deployed Grey Relational Analysis to determine which anthocyanin was the key antioxidant component in the nine anthocyanins. The relation between the ROS scavenging activity and the 9 anthocyanin content was established. Our results suggest cyanidin-3,5-diglucoside has the greatest correlation to the ROS scavenging activity of the anthocyanin extractions.

EFFECT OF BLACKCURRANT AND KAMCHATKA HONEYSUCKLE EXTRACTS ON QUALITY PROPERTIES OF RAW-COOKED MEAT PRODUCT

In recent years we notice customers demand for substitution of synthetic food additives, such as antioxidants, for natural and possibly healthier options. Lipid oxidation is the most important meat deterioration process. To retard the oxidation meat industry use variety of synthetic antioxidants. However, due to recent customer demands, industry is trying to replace those synthetic antioxidants for their natural counterparts. This is a challenging process because not only new additives must adequate antioxidant ability, but it also cannot alter sensory quality of products. Fruit extract seems to provide such an option. In our study, blackcurrant (Ribes nigrum L.) and Kamchatka honeysuckle (Lonicera caerulea var. kamtschatica) was incorporated into raw-cooked meat products (Frankfurters). Frankfurters were vacuum packed and stored at 4 °C for 21 days. During the storage period no significant differences in pH was recorded, with average value of 5.54. Also texture analysis did not reveal differences between the control and experimental groups. Both extracts decrease the amount of malondialdehyde (MDA) created in samples. Control groups increase of MDA levels at the end of storage was 108%, blackcurrant and honeysuckle extract groups were just 44 and 39% respectively. Sensory evaluation however revealed that honeysuckle extract altered taste of final product in significant way. This could pose a problem with customers’ acceptance of such a product.

Haskap Berry Leaves (Lonicera caerulea L.)-The Favorable Potential of Medical Use.

The presented research evaluates the medical use potential of Lonicera caerulea leaves, which are waste plants in cultivating berries. The study’s screening activity included the leaves of five varieties of Lonicera caerulea: Atut, Duet, Wojtek, Zojka, and Jugana. The microbiological analysis confirmed the safety of using Lonicera caerulea leaves without significant stabilization. Lonicera caerulea leaves standardization was carried out based on the results of the chromatographic analysis, and it showed differences in the contents of active compounds (loganic, chlorogenic and caffeic acids, and rutin), which are attributed to biological activity. For the Lonicera caerulea leaves varieties tested, the differences in the content of total polyphenol content, chlorophylls, and carotenoids were also confirmed. The screening of biological activity of five Lonicera caerulea leaf varieties was carried out concerning the possibility of inhibiting the activity of α-glucosidase, lipase, and hyaluronidase as well, and the antioxidant potential was determined. The defined profile of the biological activity of Lonicera caerulea leaves makes it possible to indicate this raw material as an essential material supporting the prevention and treatment of type II diabetes. However, this research showed that tested enzymes were strongly inhibited by the variety Jugana. The health-promoting potential of Lonicera caerulea leaves was correlated with the highest chlorogenic acid and rutin content in the variety Jugana.

Polyphenols Content, Antioxidant Properties and Allergenic Potency of Organic and Conventional Blue Honeysuckle Berries

Blue honeysuckle berries are a rich source of polyphenols with strong antioxidant properties. The purpose of this research was to determine the effect of organic and conventional cultivation on the polyphenols, antioxidant and allergenic potency of blue honeysuckle berry cultivars: ‘No 30’, ‘Jolanta’ and ‘Indygo’ in two growing seasons. Identification of individual polyphenols was performed using the HPLC method; the total polyphenols content and antioxidant activity were determined by spectrophotometric methods. The determination of allergic potency was tested by ELISA. In the second year of the study the total polyphenols were significantly higher in organic blue honeysuckle than in the conventional blue honeysuckle. In both growing seasons, the ‘Indygo’ cv. was characterized by the highest concentration of all bioactive compounds 3241.9 mg and 3787.2 mg per 100 g−1 D.W. A strong correlation was found between the polyphenol content and the antioxidant activity for organic fruit in both years, as well as for allergenic potency. Contrary to the best bioactive properties was ‘Indigo’ cv., with the highest allergenic potency (108.9 and 139.2 ng g−1 D.W.). The lowest content of specific allergens was found in the ‘No 30’ cv. Since honeysuckle is still a new cultivated plant, information about its allergenic potency is insufficient.