Point Of Harvest Research Articles

Aplicação de melatonina e sacarose no prolongamento da vida de vaso de flores de corte de amarilis (Hippeastrum hybridum Herb)

Abstract The effects of melatonin application by itself and mixed with sucrose solution on vase life and physicochemical changes in amaryllis cut flowers were investigated. Amaryllis stems with flower buds at harvest points were placed into vases containing different maintenance solutions: Melatonin (Mt; 0.01 mM), Sucrose (Suc; 4%), Melatonin + Sucrose (Mt + Suc; 0.01 mM + 4%) and distilled water (Control). Each treatment consisted of five repetitions, with one inflorescence stem per vase. The vases remained at 22 ± 3 °C and constant lighting of 13 µmol m-2 s-1 for 14 days. Immersion of stems in Suc or Mt + Suc delayed the initial senescence processes of the amaryllis cut flower. Vase life was prolonged for additional 2 days compared to stems immersed in water (control), increasing from 10 to 12 days of conservation. Floral stem mass was kept more stable; the degradation of anthocyanin and flavonoid pigments was delayed; and the stability of phenolic compounds and total carbohydrates was maintained for 14 days. These findings confirm the role of melatonin as a preservative in cut flowers. However, its preservative effect was potentialized by the addition of sucrose to the water of the amaryllis cut flowers. More work needs to be carried out to investigate the physiological mechanisms promoted by the interaction of melatonin and sucrose in the regulation of senescence in amaryllis cut flowers, including the analysis of gene expression and activity of the antioxidant system.

HARVEST POINT AND SENSORY QUALITY OF BRS SOL BAHIA: NEW PINEAPPLE HYBRID RESISTANT TO FUSARIOSIS

Knowing the appropriate harvest point is essential for the producer to guarantee the quality of fruits to be offered to consumers. This study aimed at evaluating the influence of the harvest point on the physical-chemical and sensory qualities of fruits of a new pineapple cultivar, BRS Sol Bahia, obtained by the breeding program of Embrapa Cassava & Fruits. This cultivar has been registered at the National Cultivar Registration of the Brazilian Ministry of Agriculture (RNC-MAPA) and is in the final validation phase for technical recommendation for cultivation. The fruits were harvested at the following maturation stages: “green ripe” (completely green skin), “painted” (up to 25% of yellow-orange skin), “colored” (with up to 50% of completely yellow skin) and “yellow” (more than 50% of yellow skin) and evaluated for mass and dimensions, physical-chemical characteristics, sensory acceptance of color, aroma, flavor, texture/firmness, overall acceptance, intensity of sweetness, acidity and firmness attributes and purchase intention. Fruit mass and soluble solids (SS) contents and the SS/titratable pulp acidity ratio increased with advancing maturation. Most consumers preferred the acidity (53%) and sweetness (63%) of the fruits in the “colored” stage and their texture in the “yellow” stage (75%). The physical-chemical characteristics and the sensory quality of the fruits indicate the “colored” stage as the ideal harvesting point for fruits of this pineapple cultivar. However, this result needs to be confirmed by studies on the resistance to transport and the conservation of the fruits in the post-harvest phase until reaching the consumer's table.

Prototype Network for Predicting Occluded Picking Position Based on Lychee Phenotypic Features

The automated harvesting of clustered fruits relies on fast and accurate visual perception. However, the obscured stem diameters via leaf occlusion lack any discernible texture patterns. Nevertheless, our human visual system can often judge the position of harvesting points. Inspired by this, the aim of this paper is to address this issue by leveraging the morphology and the distribution of fruit contour gradient directions. Firstly, this paper proposes the calculation of fruit normal vectors using edge computation and gradient direction distribution. The research results demonstrate a significant mathematical relationship between the contour edge gradient and its inclination angle, but the experiments show that the standard error projected onto the Y-axis is smaller, which is evidently more conducive to distinguishing the gradient distribution. Secondly, for the front view of occluded lychee clusters, a fully convolutional, feature prototype-based one-stage instance segmentation network is proposed, named the lychee picking point prediction network (LP3Net). This network can achieve high accuracy and real-time instance segmentation, as well as for occluded and overlapping fruits. Finally, the experimental results show that the LP3Net based on this study, along with lychee phenotypic features, achieves an average location accuracy reaching 82%, significantly improving the precision of harvesting point localization for lychee clusters.

Reducing food-related economic loss to improve food security and cattle trade in the Sahel: the case of agropastoral systems in Senegal

Food loss is a critical issue in Africa, but investigation has mainly been limited to quantity loss. Economic losses are likely to be more significant but are widely ignored. Regarding ruminant-related losses, it remains challenging to identify the optimal harvest point. Focusing on Sahelian agropastoral systems, where stakeholders operate in a shock-prone environment, our paper explains how critical actor behaviour is, and it addresses economic losses on live-animal transactions while integrating market behaviours into the analysis. Loss elimination being illusory in such a context, our findings pioneer a loss reduction approach that is supported by an appropriate optimisation programme tested on primary data collected from 202 agropastoral households in Senegal.

Vacuolar localisation of anthocyanin pigmentation in microgreen cotyledons of basil, cabbage and mustard greens does not impact on colonisation by Shiga-toxigenic Escherichia coli O157:H7

Microgreens, the immature plants harvested after a few weeks of growth, are perceived as a heathy, nutritious food ingredient but may be susceptible to colonisation by human pathogens including Shiga-toxigenic Escherichia coli (STEC). Some microgreen cultivars accumulate anthocyanins or secrete essential oils which, when extracted or purified, have been reported to inhibit bacterial growth. Therefore, the impact of anthocyanins on bacterial colonisation by STEC (Sakai) was compared for three species that have pigmented cultivars: basil (Ocimum basilicum L.), cabbage (Brassica oleracea L.) and mustard greens (Brassica juncea L.). Inoculation with low concentrations of STEC (Sakai) (3 log10 colony forming units/ml (CFU/ml)) during seed germination resulted in extensive colonisation at the point of harvest, accumulating to ∼ 8 log10 CFU/g FW in all cultivars. Bacterial colonies frequently aligned with anticlinal walls on the surface of epidermal cells of the cotyledons and, in basil, associated with peltate and capitate gland cells. Crude lysates of pigmented and non-pigmented basil cultivars had no impact on STEC (Sakai) growth rates, viability status or biofilm formation. Anthocyanins are located within plant vacuoles of these microgreen cultivars and did not affect colonisation by STEC (Sakai) and pigmentation therefore cannot be considered as a controlling factor in bacterial interactions.

First report of Lasiodiplodia brasiliensis causing crown rot on banana in Brazil.

Postharvest diseases compromise banana quality and cause high economic losses in Brazil. Among them, the crown rot prevails and its causal agents belong to distinct fungal species such as Colletotrichum musae (Berk. & Curt.) von Arx, Fusarium spp., and Lasiodiplodia theobromae (Pat.) Griff. & Maubl. (Griffee and Burden 1976; Ploetz et al. 2003). Symptoms of crown rot were observed on banana fruits of cv. Williams in a commercial area in Assu, Rio Grande do Norte, Brazil (04°54'0.06"S, 37°22'6.02"W) in 2017. The samples were collected, superficially disinfected with NaClO (2%), and incubated in a wet chamber at 25 °C, with a 12 h photoperiod, for approximately 3 days. After the appearance of disease symptoms and pathogen signs, mycelia were transferred from the lesions to obtain pure cultures on a potato dextrose agar (PDA) medium. Thus, a monosporic culture was obtained (isolate BAN82). The fungus produced pycnidia with conidia on potato carrot agar (PCA) culture medium containing pine needles, after four weeks of incubation at 28 °C. The conidia were hyaline when immature and brown with central transverse septum when mature. The presence of conidiogenous cells, paraphyses, and conidiophores also were observed. The conidia present ovoid format measuring 20-28 x 11-14 µm (n=50). The fungal colony produced abundant aerial mycelia of mouse grey coloration, progressing to dark mouse grey (Rayner 1970), on PDA for 15 days to 28 °C. The growth rate was 29.3 mm/day on PDA. The genomic DNA was extracted and amplified PCR with primers TEF1-688F/TEF1-1251R, ITS1/ITS4, and Bt2a/Bt2b and sequenced in both directions. The TEF1 and TUB2 sequences showed 100%, and the ITS showed 93.06% identity with the sequences of Lasiodiplodia brasiliensis (GenBank accession numbers: ON623895, TEF1, ON623896, TUB2, and ON599012, ITS. Multiple alignments of the combined dataset of the isolate and representative sequences obtained from GenBank were submitted phylogenetic analyses to bayesian inference (IB) with posterior probabilities of 10,000,000 generations. The morphological characteristics together with multigenic analysis of the three genomic regions made it possible to identify the BAN82 isolate as Lasiodiplodia brasiliensis, showing bootstrap support of posterior probabilities of 0,98 in the IB analysis. The pathogenicity was evaluated on 16 banana fruits from cv. Prata Catarina, at the point of harvest. For inoculation, the bananas were disinfected with water, soap, and, NaClO (2%). Posteriorly, the fruits were wounded on both ends, followed by the deposition of 5mm diameter mycelial plugs from the fungal culture, within 7 days of the growth. After the inoculation, the fruits were incubated in plastic boxes in a wet chamber at 25 °C, with 12 h photoperiod, for 3 days. To complete Koch's postulates, the isolate was inoculated again into 16 other banana fruits from cv. Prata Catarina. The negative control fruits were not inoculated with the pathogen, only with PDA discs. The BAN82 isolate was pathogenic to the banana cv. Prata Catarina. In the Brazilian Northeast, L. brasiliensis was described in 2014 as being associated with papaya stem rot. Up to the moment, there are no reports of L. brasiliensis as the causal agent of crown rot on bananas from Brazil (Netto et al. 2014; Farr and Rossman 2022). Thus, our work is the first to report L. brasiliensis causing crown rot on banana fruits cv. Prata Catarina in Brazil.

First report of Lasiodiplodia iraniensis causing crown rot on banana fruits in Brazil.

Banana is a fruit of great importance in Brazil and crown rot cause considerable damage and losses (Ploetz et al. 2003). The disease is associated with fungal complexes, especially the Lasiodiplodia theobromae sensu lato (Kamel et al. 2016; Renganathan et al. 2020; Waliullah et al. 2022). Three asymptomatic bunches of banana cv. 'Prata Catarina' were collected in Russas, Brazil (04°58'11.6"S, 38°01'44.5"W), in 2017. The samples were disinfected (NaClO, 200 ppm), and incubated in a moist chamber at 28 °C, with 12 h light/12 h dark for 3 days. With the appearance of the symptoms (32% of severity), the isolation was conducted in potato dextrose agar (PDA). A monosporic culture (BAN14) was obtained from a typical crown rot lesion, which was subjected to morphological characterization, showing abundant aerial mycelium of olivaceous grey color on the surface and greenish grey on the back (Rayner 1970) in PDA after 15 days at 28 °C. The growth rate was 28.2 mm. day-1. The fungus produced pycnidia and conidia on water agar medium containing pine needles, with 3-4 weeks at 28 °C, presenting conidia initially aseptate, subglobose to subcylindrical, becoming pigmented with 1-central transverse septum and longitudinal striations 23.5 (18.7) 26.0 x 12.7 (9.7) 14.8 µm (n=50). Paraphyses, hyaline, cylindrical, thin-walled, apparently coenocytic with rounded apex, with length and width dimensions of 34 (43.8) 53.2 x 2.1 (2.5) 3.2 µm (n=30). Conidiophore absent, conidiogenous cells hyaline, smooth and with thin walls. The genomic DNA was extracted and amplified by PCR with primers TEF1-688F/TEF1-1251R, ITS1/ITS4, and Bt2a/Bt2b, and sequenced in both directions (O'Donnell et al. 1998; O'Donnell et al. 2010) (GenBank accession ON975017 [TEF1], ON986403 [TUB2], and ON921398 [ITS]). BLASTn analysis of TEF1, TUB2 and ITS sequences in NCBI database showed 99 to 100% nucleotide identity to a representative isolate of Lasiodiplodia iraniensis (IRAN921). Phylogenetic analysis using maximum parsimony based on the combined TEF1, TUB2 and ITS sequences indicated that the BAN14 formed a supported clade (82% bootstrap value) to L. iraniensis. The pathogenicity was evaluated in 20 banana fruit cv. 'Prata Catarina', at the point of harvest. For inoculation, the bananas were washed with water and soap, and disinfected with NaClO (200 ppm). Posteriorly, two wounds were made on the extremities of the fruits, in which were deposited mycelial discs of 5 mm in diameter, with 7 days of the growth on PDA. After inoculation, the fruits were incubated in plastic boxes in a wet chamber at 25 °C, with 12 h light/12 h dark for 5 days. The control fruits were not inoculated with the pathogen, only with PDA discs. The experiments were repeat twice. The BAN14 isolate was pathogenic to the banana cv. 'Prata Catarina'. The BAN14 was grouped with the species L. iraniensis described by Abdollahzadeh et al. (2010) in Iran. This species is distributed in Asia, South and North America, Australia, and Africa. In Brazil it was reported in association to Anacardium occidentale, Annona muricata, A. squamosa, Annona ×cherimola-squamosa, Citrus sp., Eucalyptus sp., Jatropha curcas, Mangifera indica, Manihot esculenta, Nopalea cochenillifera, Vitis sp. and V. vinifera. Until the moment, there is not description of the relation between banana crown rot and L. iraniensis (Farr and Rossman 2022). Our work is the first report on the pathogenicity of this species on banana fruit cv. 'Prata Catarina' worldwide.

Mobile Robot System for Selective Asparagus Harvesting

Asparagus harvesting presents unique challenges, due to the variability in spear growth, which makes large-scale automated harvesting difficult. This paper describes the development of an asparagus harvesting robot system. The system consists of a delta robot mounted on a mobile track-based platform. It employs a real-time asparagus detection algorithm and a sensory system to determine optimal harvesting points. Low-level control and high-level control are separated in the robot control. The performance of the system was evaluated in a laboratory field mock-up and in the open field, using asparagus spears of various shapes. The results demonstrate that the system detected and harvested 88% of the ready-to-harvest spears, with an average harvesting cycle cost of 3.44s±0.14s. In addition, outdoor testing in an open field demonstrated a 77% success rate in identifying and harvesting asparagus spears.

Hydroalcoholic extracts of Psidium cattleianum Afzel. ex Sabine for liqueur production: influence of different fruit harvesting points on phytochemical, volatile, and sensory profiles, antioxidant activity and physical characteristics

Hydroalcoholic extracts of Psidium cattleianum Afzel. ex Sabine for liqueur production: influence of different fruit harvesting points on phytochemical, volatile, and sensory profiles, antioxidant activity and physical characteristics

Quantification and Distribution of Primary and Secondary Metabolites in the Inner and Outer Parts of Strawberry Fruit

The distribution of primary and secondary metabolites within a fruit can affect its nutritional and organoleptic quality, as fruit can vary in size and shape. This study investigated the differences in the distribution of the primary and secondary metabolites in the fruit of four strawberry cultivars (‘Asia’, ‘Clery’, ‘Frederica’, and ‘Sandra’) that were collected at one harvest point. The study included an analysis of the individual sugars, organic acids, phenolic compounds, and enzymes responsible for the degradation of phenolics. All of the studied cultivars showed a lower pH, higher total organic acid content, and lower glucose and fructose content in the outer part of the fruit. Differences were also observed in the total phenolic and anthocyanin contents, which were always higher in the outer part. The absolute differences in the total phenolic content ranged from 3723 to 6154 mg kg−1 dry weight. Our results provide a basis for understanding the differences in the biosynthesis of these metabolites within this fruit and prove that it is essential to mix samples well before extractions to obtain results that are representative of the whole fruit.

Vegetation Indices for Predicting the Growth and Harvest Rate of Lettuce

Urbanization has provided greater demand for food, and the search for strategies capable of reducing waste is essential to ensure food security. Lettuce (Lactuca sativa L.) culture has a short life cycle and its harvest point is determined visually, causing waste and important losses. Using vegetation indices could be an important alternative to reduce errors during harvest definition. The objective of this study was to evaluate different vegetation indices to predict the growth rate and harvest point of lettuce. Twenty-five genotypes of biofortified green lettuce were evaluated. The Green Leaf Index (GLI), Normalized Green Red Difference Index (NGRDI), Spectral Slope Saturation Index (SI), and Overall Hue Index (HUE) were calculated from images captured at 1, 8, 18, 24, and 36 days after transplanting (vegetative state). The diameter and average leaf area of plants were measured using QGIS software. Green mass, number of leaves, and plant and stem diameter were measured in the field. The means were compared using the Scott–Knott test (p ≤ 0.05) and simple linear regression models were generated to monitor the growth rate, obtaining R2 values ranging from 62% to 99%. Genetic dissimilarity was confirmed by the multivariate analysis presenting a cophenetic correlation coefficient of 88.49%. Furthermore, validation between data collected in the field versus data obtained by imaging was performed using Pearson’s correlations and showed moderate to high values. Overall, the vegetation indices SI, GLI, and NGRDI were efficient for monitoring the growth rate and determining the harvest point of different green lettuce genotypes, in attempts to reduce waste and losses. It is suggested that the definition of the harvest point based on vegetation indices are specific for each genotype.

Influence of maturity and harvest region on the chemical composition and physicochemical properties of oils derived from macauba fruits

Macauba (Acrocomia aculeata) is a good source of vegetable oil in tropical America. Its fruits are highly suitable for biodiesel, cosmetics and food production owing to the high quality of its oil. However, the influence of maturity and harvest region on the quality of oils is not known. Thus, the chemical composition and physicochemical properties oils extracted from the macauba palm fruit at two regions of Minas Gerais and different maturity stage were investigated. C16:0 and C18:1 were the most abundant fatty acids in the mesocarp oil. C12:0, C14:0, C16:0, and C18:1 prevailed in the kernel oil. The High-Resolution Gas Chromatography analysis revealed triacylglycerols (TGs) with equivalent carbon numbers (CN) ranging between 28 and 54. TGs composed of long-chain fatty acids prevailed in the mesocarp oil (CN52 and CN54). On the other hand, the kernel showed a more complex lipid profile, containing TGs with CN between 30 and 54. The lipid content in the oils increased significantly with the ripening of the fruit and the harvest regions. Furthermore, changes in physicochemical properties were observed for both oils depending on the stage of maturity and harvest point. Macauba oils can be used in several industries, such as food and cosmetics. Thus, for its best use, the physicochemical properties of greatest interest should be evaluated in order to identify the ideal cultivation region and maturity stage.

Erythroprotective Potential of Phycobiliproteins Extracted from Porphyridium cruentum.

There are multiple associations between the different blood groups (ABO and RhD) and the incidence of oxidative stress-related diseases, such as certain carcinomas and COVID-19. Bioactive compounds represent an alternative to its prevention and treatment. Phycobiliproteins (PBP) are bioactive compounds present in the microalga Porphyridium cruentum and, despite its antioxidant activity, their inhibitory effect on hemolysis has not been reported. The aim of this work was to evaluate the erythroprotective potential of phycobiliproteins from P. cruentum in different blood groups. The microalga was cultured in F/2 medium under controlled laboratory conditions. Day 10 of culture was determined as the harvest point. The microalgal biomass was lyophilized and a methanolic (MetOH), Tris HCl (T-HCl), and a physiological solution (PS) ultrasound-assisted extraction were performed. Extract pigments were quantified by spectrophotometry. The antioxidant activity of the extracts was evaluated with the ABTS+•, DPPH•, and FRAP methods, finding that the main antioxidant mechanism on the aqueous extracts was HAT (hydrogen atom transfer), while for MetOH it was SET (single electron transfer). The results of the AAPH, hypotonicity, and heat-induced hemolysis revealed a probable relationship between the different antigens (ABO and RhD) with the antihemolytic effect, highlighting the importance of bio-directed drugs.

Grapevine red blotch virus alters grape skin cell-wall composition impacting phenolic extractability during winemaking.

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening. However, grape cell-wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large role in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking. Understanding the impact of GRBV infection on cell-wall metabolism is important for the development of potential mitigations strategies. In this study, high-throughput transcriptome sequencing was conducted on Vitis vinifera L. 'Merlot' grapes during ripening. The cell-wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined. Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell-wall solubilization and degradation. However, these observations did not translate to changes in cell-wall composition at either harvest point in diseased grapes, potentially suggesting post-transcriptional regulation. Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis-related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest. Both pectin and pathogenesis-related proteins are known to retain phenolic compounds during winemaking. Our study corroborates this finding when the percentage extractability of flavonols in wines was significantly lower when made from GRBV-infected fruit. These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking. © 2023 Society of Chemical Industry.

Precision Agriculture Using Soil Sensor Driven Machine Learning for Smart Strawberry Production.

Ubiquitous sensor networks collecting real-time data have been adopted in many industrial settings. This paper describes the second stage of an end-to-end system integrating modern hardware and software tools for precise monitoring and control of soil conditions. In the proposed framework, the data are collected by the sensor network distributed in the soil of a commercial strawberry farm to infer the ultimate physicochemical characteristics of the fruit at the point of harvest around the sensor locations. Empirical and statistical models are jointly investigated in the form of neural networks and Gaussian process regression models to predict the most significant physicochemical qualities of strawberry. Color, for instance, either by itself or when combined with the soluble solids content (sweetness), can be predicted within as little as 9% and 14% of their expected range of values, respectively. This level of accuracy will ultimately enable the implementation of the next phase in controlling the soil conditions where data-driven quality and resource-use trade-offs can be realized for sustainable and high-quality strawberry production.

VARIATION OF GROWTH AND YIELD OF CHILLI WITH DIFFERENT DOSES OF NITROGEN IN LAMJUNG DISTRICT, NEPAL

The study was undertaken to assess how the growth and yield of the NS-1701 chili variety were impacted by different nitrogen doses in the Lamjung district’s Rainas area. The study involved arranging five different treatment combinations within a Randomized Complete Block Design (RCBD), with each combination being replicated four times. The application consisted of doses (0 kilograms per hectare, 30 kilograms per hectare, 60 kilograms per hectare, 90 kilograms per hectare, 120 kilograms per hectare). Various parameters related to plant growth and yield were observed throughout the study timeframe. The data underwent statistical analysis utilizing Microsoft Excel and R-studio software. Duncan’s Multiple Range Test (DMRT) was applied to distinguish means at a significance level of 5% for the purpose of separation. Growth characteristics such as plant height and the quantity of branches, along with yield-related attributes like fruit length, number of fruits per plant, individual plant weight, and yield (tons per hectare), displayed noteworthy variations until the point of harvest. Among varying nitrogen quantities, the most pronounced plant height (82.40 cm), branch count (8.38), fruit length (11.41 cm), fruits per plant (116.55), per-plant yield (475.5 gm), and overall yield (31.70 t/ha) were achieved with the application of 120 kilograms of nitrogen per hectare. Across all characteristics studied, the analysis of variance (ANOVA) indicated a noteworthy disparity between the various nitrogen doses concerning both yield and growth parameters. Among the range of nitrogen doses tested, the utilization of 120 kilograms of nitrogen per hectare resulted in enhanced growth and yield characteristics. This outcome could be proposed as a beneficial approach for cultivating chili in both the Lamjung district and comparable agro-ecological circumstances.

Amadurecimento de cambucis:qualidade pós-colheita e implicações na produção de compostos voláteis

Abstract Cambuci (Campomanesia phaea) is a native fruit from the Atlantic Forest, one of the richest biomes in the world. The cambuci has soft and astringent pulp, green coloration and is characterized as an aromatic fruit. This is the first study that describes the quality evolution of cambucis during their ripening. Quality parameters were evaluated at three harvesting points: weight loss, pulp firmness, skin color, total soluble solids, titratable acidity, ascorbic acid, total soluble sugars, total phenolic compounds, soluble and total tannins, respiratory activity, ethylene production, and volatile compound profiling. Fruit harvested in stages 1 somewhat rounded equatorial region) and 2 (increasingly rounded equatorial region) of ripening had two additional days of postharvest life. A decrease in firmness was observed over the days, demonstrating desirable pulp softening. No increase in ethylene production associated to respiratory peaks were observed. Volatile profile changed according to ripeness, and early harvested fruit was able to produce partially volatile compounds found in ripe fruit.

Pectoralis major flap for pharyngocutaneous fistula after total laryngectomy- two different approaches

Introduction. The reconstruction of large postoperative defects after oncologic surgery of the head and neck remains challenging. Regional flaps are considered a less expensive reconstructive option compared to free flaps. The pectoralis major flap is one of the most versatile choices for the reconstruction of large head and neck defects. Case report. We present technical key points for safe harvesting of pectoralis major flap for two cases in a university-affiliated tertiary care medical center. Both patients were male, with an average age of 64 years. The defects that required reconstruction in Case 1 were on the lateral neck region and, in Case 2, on the anterior side of the neck. Flaps were used for covering the pharyngocutaneous fistula after total laryngectomy and irradiation. The donor site was closed primarily. Flaps in both patients healed primarily without complications. Conclusion. The pectoralis major flap has a constant vascular pedicle and can successfully be used for the reconstruction of large head and neck defects. In order to obtain absolute flap survival, the operative technique must be impeccable.

The Drivers of Food Loss at the Farm Level: A Study of Farmers in Salatiga, Central Java, Indonesia

Food loss and waste (FLW) contribute to adverse economic and environmental impacts. In Indonesia, the agriculture sector is the primary source of income for 38,78 million people, and food loss is a significant issue. At the farm level, food loss falls into two categories: food that is never harvested and food that is lost between the point of harvest and sale. The term “food loss” is frequently used to refer to agricultural production that is lost unintentionally. This research aims to identify the drivers of food loss at the farm level in Indonesia using a case study of farmers in Salatiga City, Central Java Province of Indonesia. The farmers’ survey was distributed in Salatiga’s most significant urban village (Sidorejo Lor), with n=22 farmers. Using descriptive quantitative analysis, the study found that farmers are not well informed about the issue of food loss. The food loss drivers at the farm level include the presence of pests and disease, human error during manual harvesting, and gaps in appropriate harvesting technology, which results in substantial crop loss.

Temporal trends in CO2 emissions from Picea rubens stumps: A chronosequence approach

Understanding the forest carbon cycle has become increasingly important as carbon dioxide (CO2) emissions contribute to the changing climate. Decomposition is a major component of the forest carbon cycle; however, aspects of wood decomposition remain poorly understood, especially for stumps. To fill this knowledge gap, we examined the change in CO2 emissions over time from Picea rubens Sarg. (red spruce) stumps using a 32-year chronosequence (i.e., 0, 2, 4, 8, 23, and 32 years since harvest) derived from detailed harvesting records in a northern conifer forest in central Maine, USA, that has experienced repeated partial harvests. We found low initial CO2 flux (3.2 μmol CO2 m−2 s−1 at year 0) followed by a rapid increase, peaking 8 years post-harvest (24.3 μmol CO2 m−2 s−1) followed in turn by a decrease to very low rates by years 23 and 32 (1.4 and 1.7 μmol CO2 m−2 s−1, respectively). We found no clear relationship between CO2 emissions and any of the environmental or stump variables tested (wood temperature, wood moisture, soil moisture, and/or stump volume), suggesting that time since harvest was the overriding influence on CO2 flux rates. The large variability in CO2 flux rates among stumps of the same time since harvest points to the need for future research that includes larger sample sizes and covers a wider range of environmental and stump variables to better capture potential sources of variation. Our results add to the growing body of research on carbon emissions from deadwood that can inform forest carbon-cycle models. In addition, forest managers, who are increasingly interested in carbon management, can use these results to assess harvesting impacts on forest carbon emissions.