Changing climate conditions are influencing quality and yield of the United States rice (Oryza sativa L.) export industry. The occurrence of high heat episodes, along with rising atmospheric carbon dioxide (CO2), is of particular concern for as-yet uncharacterized hybrid varieties. A popular rice hybrid was grown in soil-plant-atmosphere-research (SPAR) chambers under two CO2 levels (450 or 740 ppm) and subjected to one of three day/night temperature regimes during anthesis and grainfill: a control temperature (T) (0C) of 28/22°C, and a +4° (4C) or +8°C (8C) increase. There was little difference in biomass between 0 and 4C levels; however, yields at the 8C level declined by over 50%, by about 28 or 34 g plant−1°C−1 for ambient or elevated CO2 grown rice, respectively. There was little difference in fraction of biomass allocated among leaf, stem or panicle at 0 or 4C, but the proportion declined by about 31% to the panicles at 8C. Total productive tiller numbers and grainfill percentages were similar across CO2 levels at each T treatment such that yield declines were associated primarily with a decrease in grain number and mass. Slightly higher values for yield and harvest index were observed under elevated CO2 at the 3 T levels which was attributed to an average 10 mg increase in single grain weight. An interaction of CO2 and T on chalk content was observed, with expression at the +4°C treatment 11% higher for elevated versus ambient CO2; however, chalk expression was similar for CO2 levels at the +8°C treatment. Breeding efforts associated with climate resiliency should consider traits associated with hybrid vigor as well as high temperature resistance for grain sterility and quality factor responses.
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