Harmful Chemicals Research Articles

Green Electrochemical Sensors, Their Applications and Greenness Metrics Used: A Review

AbstractGreen analytical methods are important techniques that aim to reduce or eliminate the use of harmful chemicals and waste in various analyses. These methods, based on green chemistry principles, are environmentally friendly and reliable. Electrochemical techniques have an essential role in diverse analyses, and are presented as a superior alternative to existing analytical devices in terms of different parameters. In the present review, we have comprehensively covered electrochemistry‐based sensors that are developed using green chemistry principles and evaluated their performance characteristics and specific working conditions. We here included sensors developed based on potentiometry and voltammetry methodologies, and detailed their greenness potentials based on certain metrics. Considering the advantages provided by these environmentally friendly new generation sensors, it will be inevitable for researchers working in this field to direct their attention to sensors that can be produced with green approaches.

Tetranychus urticae Koch Biology on Apple Trees

The two-spotted spider mite (Tetranychus urticae Koch) is a polyphagous arthropod herbivore that consumes over 1,100 plant species from 140 distinct plant families, including those producing harmful chemicals in temperate and tropical climates. It is an important pest in greenhouse and field cultivation, causing damage to various crops such as citrus, apples, grapes, tomatoes, peppers, cucumbers, strawberries, maize, and soy. T. urticae ability to spread over agricultural fields and adapt to environmental stressors is linked to its high propensity for mutation, leading to the highest frequency of pesticide resistance among arthropods. The genetic diversity of T. urticae gathered from apple orchards throughout Poland has been studied to investigate potential correlations between molecular variations and the occurrence of resistance to METI-acaricides. Research has shown that an abundance of leaf pubescence on the abaxial leaf surface adversely affects the development of European red mite, two-spotted spider mite, and carmine spider mite. However, due to variations in leaf sizes, tree canopy, tree ages, and the existence of natural enemies, it is impossible to compare data gathered due to variations in leaf sizes, tree canopy, tree ages, and the presence of natural enemies. Pesticide treatments negatively impact fruit plantations' ecosystems, devastating biodiversity, altering the makeup and structure of arthropod populations, and promoting mass reproduction of phytophage mites by displacing certain species with others. The most extensive research has been done on the common spider mite's development of resistance to avermectin group preparations, with some studies showing resistance to multiple acaricides.

Advancements in green-synthesized transition metal/metal-oxide nanoparticles for sustainable wastewater treatment: techniques, applications, and future prospects

Abstract In order to meet the demand for portable water and replenish depleting water resources caused by industrialization, urbanization, and population growth, wastewater purification has become crucial. Emerging contaminants (ECs), which include organic dyes, pesticides, pharmaceutical drugs, polyaromatic compounds, heavy metal ions, and fertilizers, among others, have caused significant disruptions to environmental balance and severe health complications. As a result, considerable effort has been devoted to the development of technologies that eliminate wastewater from effluents via adsorption, photocatalysis, and other means. However, considering the economic and environmental implications of the adopted technologies, green technology has gained significant attention owing to their eco-friendly approaches, cost-effectiveness, avoiding use of toxic and harmful chemicals and production of less-toxic by-products. Currently green-synthesized nanomaterials have seen tremendous growth in emerging as sustainable nanoadsorbents, nanocatalysts for the removal of the emerging contaminants from wastewater in highly efficient and eco-friendly manner. Thus, this review presents an overview of the various techniques utilized in wastewater treatment with a particular emphasis on the production and application of environmentally friendly transition metal/metal oxide nanoparticles as sustainable tools in wastewater treatment technology. This article also discusses the limitations and future potential of using green-synthesized transition metal/metal oxide based nanoparticles in advancing the technology on a broad scale.

Recycling of collagen from solid tannery waste and prospective utilization as adhesives.

Abstract This study explores the innovative potential of recycled collagen derived from tannery waste for use in high-performance adhesive formulations. The leather industry generates significant amounts of solid waste, primarily from chromium-tanned leather, which poses substantial environmental challenges. Recent advancements in recycling techniques have opened new avenues for repurposing this waste, particularly through collagen extraction, which comprises about 30-35% of tannery residues. This research systematically reviews the methods and applications of collagen extraction, highlighting the material’s versatility and environmental benefits when used as a bio-adhesive. The review identifies key challenges such as low water resistance, shear strength, and adhesiveness in collagen-based adhesives compared to synthetic counterparts. However, innovative solutions are emerging, including the incorporation of silane coupling agents and cross-linking technologies that significantly improve the water resistance and mechanical properties of these adhesives. Economic analyses further support the viability of using tannery waste-derived collagen in adhesive production, aligning with global sustainability goals and reducing reliance on petrochemical-based adhesives. Despite these advancements, the transition from laboratory research to commercial applications remains a significant challenge. Current studies primarily focus on small-scale experiments, with limited pilot-scale studies available. Nonetheless, the potential for collagen-based adhesives to replace harmful chemicals in industrial applications is promising, especially in sectors requiring biodegradable and non-toxic materials. This review concludes that while significant progress has been made, further research is necessary to overcome existing limitations and fully realize the commercial potential of collagen-based adhesives derived from tannery waste.

The Utilisation of Fucus vesiculosus Algae Extracts in the Production of Microgreens Hordeum vulgare L. with an Increased Content of Selected Bioactive Compounds.

Algae extracts may be a promising alternative to harmful chemicals and pesticides used commercially in the cultivation of plants with higher nutritional and health-promoting values. The cultivation of barley microgreens (Hordeum vulgare L.) was facilitated by the use of aqueous extracts from Fucus vesiculosus algae, which served as a biostimulant. Seeds for experiments were produced in accordance with EU standards, certified as organic and used to grow plants in a controlled pot experiment. A qualitative analysis of the extract, which was used to irrigate the plants, was also performed in this study, as well as stimulating properties by activating the system protecting against oxidative stress. Total phenolic content (TPC), total flavonoid content (TFV) and enzymes involved in their formation such as phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO), as well as enzymes involved in the removal of reactive oxygen species such as catalase (CAT) and superoxide dismutase (SOD), were determined in the obtained microgreen samples. Antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) was also evaluated. A noticeable increase in SOD content and antioxidant activity against DPPH was observed in barley microgreen samples after extract treatment. These results suggest that the use of extracts of this beneficial alga can enhance the antioxidant activity of the barley microgreens.

EthoCRED: a framework to guide reporting and evaluation of the relevance and reliability of behavioural ecotoxicity studies.

Behavioural analysis has been attracting significant attention as a broad indicator of sub-lethal toxicity and has secured a place as an important subdiscipline in ecotoxicology. Among the most notable characteristics of behavioural research, compared to other established approaches in sub-lethal ecotoxicology (e.g. reproductive and developmental bioassays), are the wide range of study designs being used and the diversity of endpoints considered. At the same time, environmental hazard and risk assessment, which underpins regulatory decisions to protect the environment from potentially harmful chemicals, often recommends that ecotoxicological data be produced following accepted and validated test guidelines. These guidelines typically do not address behavioural changes, meaning that these, often sensitive, effects are not represented in hazard and risk assessments. Here, we propose a new tool, the EthoCRED evaluation method, for assessing the relevance and reliability of behavioural ecotoxicity data, which considers the unique requirements and challenges encountered in this field. This method and accompanying reporting recommendations are designed to serve as an extension of the "Criteria for Reporting and Evaluating Ecotoxicity Data (CRED)" project. As such, EthoCRED can both accommodate the wide array of experimental design approaches seen in behavioural ecotoxicology, and could be readily implemented into regulatory frameworks as deemed appropriate by policy makers of different jurisdictions to allow better integration of knowledge gained from behavioural testing into environmental protection. Furthermore, through our reporting recommendations, we aim to improve the reporting of behavioural studies in the peer-reviewed literature, and thereby increase their usefulness to inform chemical regulation.

Expression of DNA repair genes in workers producing lead from recycled materials

Working conditions at lead production plants, where a large number of harmful chemicals are present in the air of the work area, among which lead is the leading risk factor, have a negative impact on the health of workers. This xenobiotic disrupts numerous biochemical processes and affects many body systems. Of particular interest is the understanding of the action of lead at the molecular level, since in addition to the general mechanisms of damage to molecules and ultrastructures as a result of oxidative stress, lead is able to bind to a large DNA groove. A change in the level of gene expression in response to toxic exposure is considered as one of the biomarkers of the effect and can serve to develop measures for the early diagnosis of health disorders in exposed populations, including those working in harmful conditions. The study aims to consider the expression of DNA repair genes in workers of lead processing from secondary raw materials. The researchers examined the interned men working at the enterprise for the production of lead from secondary raw materials in the profession of a smelter (n=11) of the rough lead department, a dryer (n=10) of the rough lead department. The comparison group included men (n=20) who work at the same enterprise and have no contact with harmful factors of production. We have performed amplification of the studied genes (ATM, CDKN1A and MDM) in real time using the QuantStudio 3 amplifier (Thermo Fisher Scientific, USA). It was found that the expression level of the ATM gene was statistically significantly different in all three study groups from the comparison group. The expression of the CDKN1A gene increased in comparison with the comparison group only in the smelters of the refining department. Also, the expression of this gene was increased in the smelters of the refining department compared with the smelters of the rough lead department. In this study, an increase in the expression level of CDKN1A in smelters of the refining department was demonstrated, which may be an adaptive mechanism of the repair system. Also, in all exposed groups, the researchers found a decrease in the expression level of the ATM gene, which is important for maintaining cellular homeostasis. Further studies of the mechanisms of the influence of lead on the expression of these two genes in experimental models will allow the use of expression levels as early biomarkers of the effect of both total Pb exposure (ATM gene) and dose-dependent (CDKN1A gene). Limitations. Ethnicity was not determined in this study. Ethics. The studies were conducted in accordance with the ethical principles set out in the Helsinki Declaration of the World Medical Association and approved by the local Ethics committee of the Federal State Budgetary Institution of the Russian National Research Center of Rospotrebnadzor (Protocol No. 1 of 02/26/2021).

The Relationship Between the Physical Condition of Water (Taste, Smell, Color) and Complaints of Skin Diseases in Simalingkar B Village

The physical condition of unclean water can cause skin disorders this is because unclean water is polluted by physical particles, pathogenic microorganisms, and harmful chemicals can cause various skin diseases. the study aims to determine the relationship between the physical condition of water (color, smell, taste) with complaints of skin disease disorders in Simalingkar B Village. In this study using quantitative methods with a cross sectional design. The population in this study was people who used well water, obtained 2,531 families using well water with sampling using the purposive sample formula so that 93 families were sampled for this study. The results of the study showed a relationship between the physical condition of water (taste, odor, color) with complaints of skin disorders in Simalingkar B Village with the results of statistical tests obtained p value 0.029 or <0.05, meaning that at 5% alpha indicates a relationship between the quality of clean water with the incidence of scabies in Simalingkar B Village. Obtained OR value = 2.815 which indicates that respondents who have a water source with physical quality of clean water that does not meet the requirements have a risk of 2.815 times to suffer from skin disorders compared to respondents who have a water source with physical quality of clean water that meets the requirements. The government and community can work together to establish clean water reservoirs to prevent skin diseases in Simalingkar B Kelurahan.

Biosynthesis of Iron Oxide Nanoparticles by Marine Streptomyces sp. SMGL39 with Antibiofilm Activity: In Vitro and In Silico Study

One of the major global health threats in the present era is antibiotic resistance. Biosynthesized iron oxide nanoparticles (FeNPs) can combat microbial infections and can be synthesized without harmful chemicals. In the present investigation, 16S rRNA gene sequencing was used to discover Streptomyces sp. SMGL39, an actinomycete isolate utilized to reduce ferrous sulfate heptahydrate (FeSO4.7H2O) to biosynthesize FeNPs, which were then characterized using UV–Vis, XRD, FTIR, and TEM analyses. Furthermore, in our current study, the biosynthesized FeNPs were tested for antimicrobial and antibiofilm characteristics against different Gram-negative, Gram-positive, and fungal strains. Additionally, our work examines the biosynthesized FeNPs’ molecular docking and binding affinity to key enzymes, which contributed to bacterial infection cooperation via quorum sensing (QS) processes. A bright yellow to dark brown color shift indicated the production of FeNPs, which have polydispersed forms with particle sizes ranging from 80 to 180 nm and UV absorbance ranging from 220 to 280 nm. Biosynthesized FeNPs from actinobacteria significantly reduced the microbial growth of Fusarium oxysporum and L. monocytogenes, while they showed weak antimicrobial activity against P. aeruginosa and no activity against E. coli, MRSA, or Aspergillus niger. On the other hand, biosynthesized FeNPs showed strong antibiofilm activity against P. aeruginosa while showing mild and weak activity against B. subtilis and E. coli, respectively. The collaboration of biosynthesized FeNPs and key enzymes for bacterial infection exhibits hydrophobic and/or hydrogen bonding, according to this research. These results show that actinobacteria-biosynthesized FeNPs prevent biofilm development in bacteria.

Measurement and health risks assessment of BTEX compounds exposure in beauty Lahijan City salons

The presence of BTEX (Benzene, Toluene, Ethylbenzene, and Xylene) compounds in beauty salons has raised concerns about potential health risks. This study aimed to measure the levels of BTEX compounds in the air of beauty salons in Lahijan, Iran and assess the associated health risks. Air samples were collected from 15 beauty salons, and the concentrations of BTEX compounds were measured according to 1501 NIOSH standard method. The results showed that the mean concentrations of benzene (20.62 µg/m3), toluene (18.3 µg/m3), ethylbenzene (38.36 µg/m3), and O and P-xylene (27.35, 23.6 µg/m3) were above the recommended levels. The indoor to outdoor ratios for benzene, toluene, ethylbenzene, O and P-xylene were 3.04, 2.36, 3.75, 4.89, and 6.54, respectively. Also, the toluene/benzene (T/B) ratio in indoor and outdoor was 20.9 and 2.68 respectively. Almost half of the technicians (49.12%) reported adverse health effects, including joint pain, itchy eyes and nose, and respiratory allergies. The IARC guideline suggests that there is a potential risk of cancer development for individuals in all salons with LCR values exceeding 10−6, but the HQ index values indicate no non-carcinogenic risk. The findings suggest that beauty salon workers and customers are at risk of developing health problems from exposure to BTEX compounds. Effective risk management strategies, such as proper ventilation, use of personal protective equipment, and substitution of harmful chemicals with safer alternatives, to minimize exposure and protect the health of salon workers and customers recommended.

Exploration of catalytic activities of Caesalpinia bonduc (L.) Roxb. ash on green-oxidation of small organic substrates: an effective way to reduce the use of harsh chemicals

In this modern era, large-scale industrial use of harsh chemicals is an alarming problem as it can cause serious environmental damage. Among industrial pollutants, harsh chemical oxidizing agents are one of the leading chemicals that can cause severe environmental threats. These harmful harsh chemicals are practically omnipresent in industries and are used to oxidize numerous small organic substrates. Replacement of these harsh chemicals with some plant-based products might be helpful to safeguard our environmental damage. In this study, Caesalpinia bonduc (L.) Roxb. ash is used as a catalyst to activate aerial oxygen for promoting the oxidation of 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAPH) like small organic substrates. Detailed kinetic studies for these catalytic oxidation reactions have been performed spectrophotometrically, which confirms that catalytic reactions follow Michaelis–Menten kinetics. Several enzyme-kinetics plots such as the Michaelis–Menten plot, Lineweaver–Burk plot, Hanes–Woolf plot, and Eadie–Hofstee plot have been used to determine the maximum rate achieved by the reaction (Vmax) and Michaelis constant (KM) like kinetic parameters. The average values of Vmax and KM for catalytic oxidation of 3,5-DTBC are (8.6669 ± 0.6519) × 10–5 M S−1 and (21.4289 ± 0.4741) × 10–4 M, respectively. Similarly, the average values of Vmax and KM for catalytic oxidation of OAPH are (2.1781 ± 0.2071) × 10–5 M S−1 and (20.1062 ± 1.2690) × 10–3 M, respectively. The mineral composition of Caesalpinia bonduc (L.) Roxb. ash has also been evaluated by using inductively coupled plasma optical emission spectroscopy (ICP-OES).Graphical

Statistical Analyses Plan for “MAGnItude of cigarette substitutioN after Initiation oF e-cigarettes and its ImpaCt on biomArkers of exposure and potenTial harm in dual users”: MAGNIFICAT trial

BackgroundThe substitution of combustible cigarettes (CC) with non-combustible nicotine alternatives, such as e-cigarettes (EC), significantly lowers exposure to harmful chemicals. However, many individuals who use ECs continue smoking CCs, becoming dual users and remaining at increased risk of toxin exposure. This study will examine how the reduction in cigarettes smoked per day (CPD) after switching to ECs correlates with changes in biomarkers of exposure (BoE) and potential harm (BoPH) to evaluate the extent of harm in dual users. MethodsThe study is a prospective, non-randomised, observational, longitudinal cohort study in which 250 CC smokers will be invited to use EC as part of a smoking reduction/cessation strategy. Participants will be grouped in five dual use categories based on the change from baseline in CPD at 1, 3 and 6 months, based on self-reported use in the preceding 30 days. The primary outcome of interest is the mean absolute urinary 2-cyanoethyl mercapturic acid (2CyEMA), a well-validated BoE, also assessed at 1, 3 and 6 months. Other BoE will be assessed, together with exploration of the association between varying intensities of dual use and BoPH. The association between the dual use category and levels of BoE will be evaluated using analysis of variance (ANOVA), in order to understand which BoE are best suited to identifying different levels of dual use. Exploratory assessment of the value of BoPH will similarly be evaluated, to assess whether their predictive power extends from the binary smoker/non-smoker categorization to a more nuanced dual use categorization. The specific details of the own-brand CC will be available once smokers' enrollment in the study is complete. The EC used in the study will be the KIWI 2 Pen refillable pod system, with participants given the option to choose from three different flavored e-liquids: LEAF, MIDWAY, and GLACIAL, all containing 0.9% nicotine. For specialized equipment used for biomarker analysis please refer our previous publication: https://pubmed.ncbi.nlm.nih.gov/38584934/ ConclusionEC are increasingly adopted as a harm reduction strategy in CC smokers who wish to quit smoking, the outlined Statistical Analysis Plan (SAP) provides a robust approach to understand the impact of EC on CC use and to quantify the impact of dual use on clinical outcomes using BoPH. For statistical calculations we will use R software.The subject of this SAP manuscript – trial research – due to planned medical procedures, is a subject of verification by proper ERB (Ethics Review Board) in Poland and gained required approval prior to the start of the actions. The positive opinion was granted on 22nd February 2024, with the signature number 04/24.

Environmentally Friendly Synthesis of Gelatin Hydrogel Nanoparticles for Gastric Cancer Treatment, Bisphenol A Sensing and Nursing Applications: Fabrication, Characterization and ANN Modeling

This study presents a dual application approach for the environmentally friendly synthesis of gelatin hydrogel nanoparticles with potential applications in gastric cancer treatment, bisphenol A (BPA) sensing, and nursing. Gelatin hydrogel nanoparticles were synthesized using a green and freeze-drying method, avoiding the use of toxic chemicals and solvents. The nanoparticles showed excellent biocompatibility and promising potential for drug delivery system (DDS) in gastric cancer treatment. The controlled release of anticancer drugs from the gelatin nanoparticles was showed, highlighting their potential in targeted therapy. Additionally, the gelatin hydrogel nanoparticles were explored for BPA sensing. BPA is a widely used chemical known for its adverse effects on human health. The gelatin nanoparticles showed high selectivity and sensitivity towards BPA detection, making them suitable for environmental monitoring and health applications using scanning electron microscope (SEM). Also, in this study, an artificial neural network (ANN) was used to estimate the release of docetaxel (%) at 72 hours, the release of paclitaxel (%) at 72 hours, tensile strength with sample (wt%), and porosity (%) in broader ranges than the experimental samples. The environmentally friendly synthesis of gelatin hydrogel nanoparticles presented in this study offers a versatile platform with dual applications in gastric cancer treatment and sensing of harmful chemicals. The obtained results show the potential of these nanoparticles for innovative therapeutic and diagnostic strategies in healthcare and environmental monitoring. The study showed the development of sustainable and multifunctional nanomaterials for various biomedical applications. The modeling of the neural network predictions shows that increasing the sample (wt%) and porosity (%) leads to an increase in the release of docetaxel (%) at 72 hours, the release of paclitaxel (%) at 72 hours, and tensile strength. As porosity decreases, the release of docetaxel increases, and the release of paclitaxel and tensile strength also increase. Additionally, the prediction errors of the ANN in this study were evaluated using linear regression, showing acceptable error rates compared to the target results obtained from the experimental tests.

Electronic cigarette use and consumption patterns in medical university students.

A major public health hazard is youth e-cigarette use. Although new, e-cigarette health hazards are becoming well-known in the literature. E-cigarette sale restrictions and laws differ globally. In this cross-sectional study, we studied medical university students' tobacco and e-cigarette use and characteristics in a country where sales and import of e-cigarettes are banned. The primary objective is to determine the prevalence of electronic cigarette use and understand consumption patterns among medical faculty students in this setting. The questionnaire was sent using a web-based student information system. Sociodemographic features, tobacco and e-cigarette use, consumption patterns, and e-cigarette risk perceptions were covered in 54 questions. The study comprised 1,054 students (48.7% male) aged 21.5 ± 2.6 years who completed the questionnaire. 37.7%, 20.9% and 23.6% have smoked cigarettes, e-cigarettes, or water pipes. Current cigarette smokers were 17.0%, e-cigarette users 4.0%, and water pipe smokers 4.5%. E-cigarette users were 52.3% dual smokers. The most common symptoms reported by e-cigarette users were cough (58.4%) and dyspnea (54.2%). Multivariable models showed that the male sex, greater monthly income, and a current smoker friend were independent risk factors for e-cigarette ever use, while the male sex, paternal current smoking, and close friends' current smoking status were risk factors for dual use among medical trainees. Many medical students who used electronic cigarettes underestimated nicotine's health hazards and harmful chemicals in e-cigarettes. Despite e-cigarette sales being prohibited in our country, 56.4% and 25.4% of e-cigarette users provided e-cigarettes from tobacco shops and through online sales, respectively. Medical university students use tobacco most often by smoking cigarettes. Despite medical university students being aware of the health hazards of e-cigarettes, the current use of electronic cigarettes is 4.0%. Male sex, greater monthly income, and having current smoker friends are independent risk factors for e-cigarette use, while paternal smoking is a risk factor for dual use among medical trainees. Although in the country, sales of e-cigarettes are banned, ever-use rates for e-cigarettes were remarkably high at 20.9%, and the ease of accessing e-cigarettes was striking.

The Impact of Nicotine Patches on Gingival and Oral Health: A Narrative Review.

Smoking significantly impacts oral health, causing periodontal disease, oral cancer, tooth discoloration, halitosis, and impaired wound healing. Nicotine replacement therapy (NRT), particularly nicotine patches, is widely used for smoking cessation. This review evaluates the literature regarding nicotine patches and their implications for oral and gum health. Nicotine patches deliver controlled nicotine doses transdermally, alleviating cravings and withdrawal symptoms. While nicotine can negatively affect oral health through vasoconstriction and reduced salivary flow, the overall impact of patches is generally positive compared to continued smoking. The primary benefit is the elimination of exposure to harmful chemicals and heat from cigarette smoke, significantly decreasing oral cancer risk and periodontal disease progression. Nicotine patches are associated with improved periodontal treatment outcomes, better wound healing, and potential anti-inflammatory effects. They may also promote improved oral hygiene practices. However, limitations include uncertain long-term effects and potential side effects like xerostomia. In conclusion, while nicotine patches may have some oral health effects, their use generally leads to significant improvements compared to continued smoking, making them valuable for promoting better oral health in individuals quitting smoking.

Voluntary self-poisoning in children – a silent call for help

Introduction. Voluntary self-poisoning represents nowadays a public health issue, being one of the main causes of morbidity and mortality in pediatric patients. The psychological burden some children have to carry becomes heavier than their strength, fact that determine them to make their voices heard through self-hurting. Aim. The current study proposed to raise awareness upon the multiple cases of voluntary ingestions between children, to assess the reasons and, consequently, to find solutions to support our future generations. Materials and methods. This is an observational retrospective study performed by analyzing the medical data of the patients admitted to the Department of Pediatrics I, from the Clinical County Emergency Hospital Targu Mures, with the diagnostic of toxic substance ingestion, between 1st of January 2019 and 31st December 2022. The study group comprised of 204 children, who were exposed to harmful chemicals, 68 of them being voluntarily involved. Results. A slight increase in the number of cases have been observed during SARS-CoV-2 pandemic. Additionally, we identified that teenage girls were more prone to self-poison than pre-school boys, who were more susceptible to unintentionally ingest toxics. Low family income, abandon, scholar difficulties, use of alcohol and pregnancy were only some of the causes of producing emotional instability. Furthermore, the most preferable substances to consume were pharmaceuticals, especially psychotropics. Conclusion. The study is emphasizing the necessity to establish mental support systems for the children who struggle with past traumas. Founding trustful connections between the child and its family, school and health care professionals can help reduce the self-harm cases and even develop a much more emotionally stronger society.

Experimental Investigation and Machine Learning Prediction of Mechanical Properties of Rubberized Concrete for Sustainable Construction

Concrete is widely used in civil engineering applications and the natural aggregates which used in concrete are scarce, but its demand is increasing. The disposal of rubber tyres poses a significant environmental challenge, as their decomposition releases harmful chemicals into the soil and water bodies over many years. Decomposition of tyres should be done in a smart way and hence came the emergence of mixing recycled rubber crumbs into concrete as Rubberised Concrete (RC). This paper provides an in-depth analysis of the mechanical properties of concrete such as Compressive Strength (fck), Tensile Strength (ft), Flexural Strength (fcr) of 7, 14, 28 days in replacement of fine aggregate with fine rubber (FR), and Coarse Aggregate with Coarse Rubber (CR). The results indicate that RC is more suitable for structural applications, including Reinforced Concrete columns, beams, slabs, than conventional concrete. The primary objective of the article is to explore the potential use of recycled rubber crumbs in concrete, referred to as Rubberised Concrete (RC), and to analyze its mechanical properties such as compressive strength, tensile strength, and flexural strength over different curing periods. Additionally, machine learning (ML) based prediction model has been developed for various strength characteristics of concrete mixtures at 28 days. The hyperparameter optimization using Grid Search CV with fivefold cross-validation have been performed to obtain the best hyperparameters. The model’s performance is evaluated using metrics like MAE, MSE, RMSE, and R-squared values. Results reveal varying performances among different ML algorithms for predicting flexural, tensile, and compressive strengths.

The nephroprotective potential of selected synthetic compound against gentamicin induced nephrotoxicity

BackgroundNephrotoxicity, the rapid impairment of kidney function caused by harmful drugs and chemicals, affects about 20% of cases and is projected to become a leading cause of death by reactive oxygen species (ROS). Gentamicin (GM), an aminoglycoside antibiotic is one of the well know drugs/chemicals to cause nephrotoxicity both in humans and animals.MethodsA study on the effects of a synthetic phenolic compound, called 5-a, on GM-induced nephrotoxicity in male Wistar albino rats was conducted. The rats were grouped into five groups: normal control (NC), GM control (GM), positive control (GM + Dexa), treatment I (GM + 5-a 5 mg/kg) and treatment II (GM + 5-a 10 mg/kg). Throughout the experiment, the rats’ weights were monitored, and at its conclusion, their serum and kidney tissues were analyzed for renal function indicators and inflammatory markers. The study also included histopathological evaluations, molecular docking studies, blood and urine analyses for electrolyte changes, and behavioural assessments for central nervous system impact.Results2-{5-[(2-hydroxyethyl)-sulfanyl]-1,3,4-oxadiazol-2-yl} phenol (5-a) significantly protected against renal damage by reducing inflammatory markers, improving antioxidant defences, and decreasing kidney injury, particularly at higher doses. The findings suggest that compound 5-a, due to its anti-inflammatory and antioxidant properties, could be a promising therapeutic option for reducing gentamicin-induced nephrotoxicity and potentially for other kidney disorders in the future.ConclusionThese findings highlight the therapeutic effects of compound 5-a in alleviating gentamicin-induced nephrotoxicity.

Soil texture analysis using controlled image processing

Soil texture analysis is crucial for crop selection, fertilizer recommendation, and production. Traditional soil testing in the lab using chemicals is highly time-consuming, expensive, and risky harmful chemicals; proper equipment and trained professionals are required to get the readings and to conduct the analysis. These issues can be resolved using image processing. In this study, we proposed a Blackbox prototype machine to take images in a controlled environment under the fixed intensity of light, distance, and standard dry conditions to analyze soil texture. This innovative machine, with its efficient and precise image processing capabilities, has the potential to revolutionize soil texture analysis. Also, we marked the center points of each type of soil texture as defined in the USDA texture triangle. Hundreds of soil samples were prepared for each type according to the center point's sand, silt, and clay ratio. The image processing-based model is trained for texture analysis. This research aims to reduce the soil texture analysis time and provide a system that can do extensive analyses automatically and with accuracy. The proposed Blackbox prototype machine has proven effective in providing a controlled environment for taking images. Also, the proposed model detects soil texture with a maximum accuracy of 99.5 %. A proposed model trained on the soil samples of different texture classes available in the USDA texture triangle accurately performed texture analysis. The results benefit the recommendation of appropriate crops and fertilizers based on a given soil sample in a very short time and cost-effectively.

An Eco-Friendly Alternative to Orange Peel, Sustainable Fashion Hair Accessories

The fashion industry contributes around 10% of global greenhouse gas emissions and 35% of plastic micro-fibers released in oceans [4][5]. This impact is amplified by the increasing demand for hair accessories, which are often made from plastic or metal and contain harmful chemicals. The global market for hair accessories is estimated to be worth Rs. 1,96,566 Crs. in 2023 and is forecasted to reach Rs. 3,50,201 Crs. by 2030 with a growth rate of 8.60%. To address the significant environmental impacts of hair fashion industry, there is a need to emphasize sustainable and eco-friendly fashion practices, including the use of organic waste. This action research project explores the use of bio-plastic made from Orange Peels, for creating sustainable and eco-friendly hair accessories. Due to high cellulose content in orange peel, bio-plastic produced using simple techniques have shown promising results, with excellent strength, flexibility, and biodegradability [1]. This research not only showcases the ‘best from the waste’ concept but also opens the door of additional income source to the orange farmers & consumers. A comprehensive online survey was conducted to get feedback from key stake holders toward promotion of eco-friendly fashion. The ‘Sakhi Paryavarnachi’ campaign has been initiated to promote the bio- plastic hair accessories amongst the end users. Overall the combined efforts of experimentation, observation, evaluation and survey based campaign have been explored herein to promote ‘Orange peel eco-friendly alternative for sustainable fashion in hair accessories.’