Aquatic macrophytes are often monitored to detect change in ecosystem function and state, as well as assessing the effectiveness of invasive aquatic plant management. This study compares seven methodologies to monitor the distribution and abundances of aquatic macrophytes. Four line transect methodologies and three spatial mapping techniques were employed in parallel over a broad turbidity gradient in two lentic habitats of south-eastern Australia. The methodologies examined included hydroacoustic surveys, on-site digitising, and digitisation of airborne remote sensing imagery. Variation in estimates of macrophyte coverage were observed between methodologies. Consistency in the collection and interpretation of data was greatest for the line transect methodologies and the digitisation of satellite imagery. Duel-frequency identification sonar proved to be an effective novel hydroacoustic technique to monitor macrophyte abundances over broad spatial scales. Single beam sonar transects was also an objective, repeatable and scalable methodology. Videography and on-site handheld PDA mapping were of limited utility due to restrictions imposed by turbidity. The utility of sidescan sonar could be improved when used in conjunction with on-site handheld PDA mapping. This study outlines important considerations when selecting a methodology to monitor macrophyte distribution and abundance. Results indicate that no one specific method can be employed across all macrophyte monitoring studies. The method or combination of methods employed during macrophyte monitoring studies is dependent upon the study objectives, budget and environmental conditions of the study site.